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The map label for this gene is trmB

Identifier: 15836517

GI number: 15836517

Start: 1133129

End: 1133803

Strand: Direct

Name: trmB

Synonym: CPj0986

Alternate gene names: 15836517

Gene position: 1133129-1133803 (Clockwise)

Preceding gene: 15836516

Following gene: 15836518

Centisome position: 92.38

GC content: 39.56

Gene sequence:

>675_bases
ATGAAACCCCAAGATCTATCCCCGCCATTTTTATGGAAAGAACGCCGTCCTTGTATTCAAGATGGAGTTCTCTATGTTCC
TAGACATTACTTTGAACACCAGAATTTTTCAACAAGCTACCATCAAGAATTTTTTCAAAATCATACTTCTATAGCTTGTG
AACTATGCTCTGGTAATGGGGATTGGGTAGTTGCTCAAGCGCAAAAAGATCCTCAAGTACTTTGGATCGCTGTGGAACAG
CGTTTTGATAGGGTAAGGAAAATTTGGTCTAAAATGATCAACCACCAGATCCAAAATTTAAGGATTGTCTGTGGTACTGC
CGAAACCTTTTTCCAGTACTATGTTCCTGATCAGTTTTTGCAACGCCTTGTTGTGAATTTTCCAGATCCCTGGCCAAAGA
TGCGGCATCGTAAACACCGTCTTCTCCAACCCTCATTCGTTCAAGAAATCTCCCGCTCTCTCCAAGATTCCGCAGTTTTT
GCTTTGGCTACCGATGATAAAACATACCTATTGGAATCCATAGAAGCGCTGCAAACACATTTAGCTCCAAGAATGGAAAC
ACCATATTATATAAAAATGACAGATACTTATGGAAATTCTTGGTTTGAAAACCTTTGGCGGACAAAGGGACAGGAAATTT
TTTATACAGAATTTATAAAAAAGGCTGGGATATAG

Upstream 100 bases:

>100_bases
TCAGGATTTTTCAGCTTTCCCTTCCGAAGAAAGTCCTTAGAAAAAACTTCAAGAAGCTTTATACTTTCCTAAAGCAAACA
GTCTCTGATTTCTGCTTTTT

Downstream 100 bases:

>100_bases
CTGACTTGAACAGCTGACCTTCACGATGTCAACGTGACGCTCTAACCAACTGAGCTAATACCCCGAGTTCAGCTACTATC
CTAACTAGAAAATGGTATCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKPQDLSPPFLWKERRPCIQDGVLYVPRHYFEHQNFSTSYHQEFFQNHTSIACELCSGNGDWVVAQAQKDPQVLWIAVEQ
RFDRVRKIWSKMINHQIQNLRIVCGTAETFFQYYVPDQFLQRLVVNFPDPWPKMRHRKHRLLQPSFVQEISRSLQDSAVF
ALATDDKTYLLESIEALQTHLAPRMETPYYIKMTDTYGNSWFENLWRTKGQEIFYTEFIKKAGI

Sequences:

>Translated_224_residues
MKPQDLSPPFLWKERRPCIQDGVLYVPRHYFEHQNFSTSYHQEFFQNHTSIACELCSGNGDWVVAQAQKDPQVLWIAVEQ
RFDRVRKIWSKMINHQIQNLRIVCGTAETFFQYYVPDQFLQRLVVNFPDPWPKMRHRKHRLLQPSFVQEISRSLQDSAVF
ALATDDKTYLLESIEALQTHLAPRMETPYYIKMTDTYGNSWFENLWRTKGQEIFYTEFIKKAGI
>Mature_224_residues
MKPQDLSPPFLWKERRPCIQDGVLYVPRHYFEHQNFSTSYHQEFFQNHTSIACELCSGNGDWVVAQAQKDPQVLWIAVEQ
RFDRVRKIWSKMINHQIQNLRIVCGTAETFFQYYVPDQFLQRLVVNFPDPWPKMRHRKHRLLQPSFVQEISRSLQDSAVF
ALATDDKTYLLESIEALQTHLAPRMETPYYIKMTDTYGNSWFENLWRTKGQEIFYTEFIKKAGI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=136, Percent_Identity=28.6764705882353, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CHLPN (Q9Z6S3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C72010
- PIR:   G86613
- RefSeq:   NP_225180.1
- RefSeq:   NP_301041.1
- RefSeq:   NP_445407.1
- RefSeq:   NP_877295.1
- ProteinModelPortal:   Q9Z6S3
- SMR:   Q9Z6S3
- GeneID:   1467702
- GeneID:   895603
- GeneID:   919748
- GeneID:   963320
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0869
- KEGG:   cpj:CPj0986
- KEGG:   cpn:CPn0986
- KEGG:   cpt:CpB1023
- TIGR:   CP_0869
- HOGENOM:   HBG413880
- OMA:   NLRIVCG
- PhylomeDB:   Q9Z6S3
- ProtClustDB:   PRK00121
- BioCyc:   CPNE115711:CP_0869-MONOMER
- BioCyc:   CPNE115713:CPN0986-MONOMER
- BioCyc:   CPNE138677:CPJ0986-MONOMER
- BioCyc:   CPNE182082:CPB1023-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26729; Mature: 26729

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 79-79 BINDING 129-129 BINDING 133-133 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQDLSPPFLWKERRPCIQDGVLYVPRHYFEHQNFSTSYHQEFFQNHTSIACELCSGNG
CCCCCCCCCCCCCCCCCHHHCCEECCCHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCC
DWVVAQAQKDPQVLWIAVEQRFDRVRKIWSKMINHQIQNLRIVCGTAETFFQYYVPDQFL
CEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHCCCHHHH
QRLVVNFPDPWPKMRHRKHRLLQPSFVQEISRSLQDSAVFALATDDKTYLLESIEALQTH
HHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHH
LAPRMETPYYIKMTDTYGNSWFENLWRTKGQEIFYTEFIKKAGI
HCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKPQDLSPPFLWKERRPCIQDGVLYVPRHYFEHQNFSTSYHQEFFQNHTSIACELCSGNG
CCCCCCCCCCCCCCCCCHHHCCEECCCHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCC
DWVVAQAQKDPQVLWIAVEQRFDRVRKIWSKMINHQIQNLRIVCGTAETFFQYYVPDQFL
CEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHCCCHHHH
QRLVVNFPDPWPKMRHRKHRLLQPSFVQEISRSLQDSAVFALATDDKTYLLESIEALQTH
HHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHH
LAPRMETPYYIKMTDTYGNSWFENLWRTKGQEIFYTEFIKKAGI
HCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362