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The map label for this gene is nrdB

Identifier: 15836516

GI number: 15836516

Start: 1131939

End: 1132979

Strand: Direct

Name: nrdB

Synonym: CPj0985

Alternate gene names: 15836516

Gene position: 1131939-1132979 (Clockwise)

Preceding gene: 15836515

Following gene: 15836517

Centisome position: 92.29

GC content: 37.85

Gene sequence:

>1041_bases
ATGGAAGCAGATATTTTAGATGGAAAGCTCAAACGGGTTGAGGTAAGTAAAAAAGGATTGGTGAATTGTAATCAAGTAGA
TGTCAATCAGCTAGTCCCTATCAAGTATAAATGGGCTTGGGAACATTACCTCAATGGATGTGCAAACAACTGGCTTCCTA
CTGAAGTTCCTATGGCAAGAGATATCGAGTTGTGGAAATCAGATGAACTGTCTGAAGACGAACGCAGGGTCATTTTGTTA
AACCTAGGATTTTTCAGTACCGCGGAAAGCCTAGTCGGAAATAACATCGTTCTTGCTATCTTCAAACATATCACAAACCC
TGAAGCAAGACAGTATTTACTGCGTCAAGCTTTTGAGGAAGCCGTACATACACATACATTTCTCTATATTTGCGAATCTT
TAGGACTTGATGAAGGCGAAGTATTCAATGCCTATAATGAAAGAGCCTCAATTAGGGCTAAAGATGATTTTCAAATGACA
TTAACAGTCGATGTCCTTGATCCTAATTTTTCTGTACAGTCTTCAGAAGGCCTTGGGCAGTTCATTAAAAACTTAGTAGG
ATACTATATCATTATGGAAGGAATCTTCTTCTATAGTGGTTTTGTAATGATTCTCTCTTTCCATAGACAAAATAAAATGA
CAGGAATTGGAGAACAGTACCAATACATCCTCAGAGATGAAACCATACATTTAAATTTTGGAATCGATCTTATCAATGGA
ATTAAAGAAGAAAACCCCGAAGTTTGGACTACGGAACTACAAGAAGAAATCGTCGCTCTTATTGAAAAAGCTGTAGAGCT
TGAAATTGAGTACGCTAAAGATTGCTTACCTCGAGGAATCTTGGGATTAAGATCTTCGATGTTTATAGATTACGTTCGTC
ATATTGCAGATCGTCGTTTAGAGAGAATTGGGTTGAAGCCTATCTATCACTCCAGAAATCCTTTCCCTTGGATGAGCGAA
ACCATGGATCTGAATAAAGAAAAGAATTTCTTTGAAACCCGGGTTACCGAATACCAAACCGCTGGTAATTTAAGTTGGTA
A

Upstream 100 bases:

>100_bases
CAGCGTCCACAAGTATTGTGGTCGAAAGAAAAACAACCCCCGTTTGTTCAATGGAAGAAGGTTGCGAATCTTGTCAATAA
CGGAAAAAAGAGGAGCTAAA

Downstream 100 bases:

>100_bases
TTTCACCTCTTTTTCAAGGGAGTTTTGAGAGATGGTCTTAGATCGTCTCTCAGGATTTTTCAGCTTTCCCTTCCGAAGAA
AGTCCTTAGAAAAAACTTCA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MEADILDGKLKRVEVSKKGLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIELWKSDELSEDERRVILL
NLGFFSTAESLVGNNIVLAIFKHITNPEARQYLLRQAFEEAVHTHTFLYICESLGLDEGEVFNAYNERASIRAKDDFQMT
LTVDVLDPNFSVQSSEGLGQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDLING
IKEENPEVWTTELQEEIVALIEKAVELEIEYAKDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYHSRNPFPWMSE
TMDLNKEKNFFETRVTEYQTAGNLSW

Sequences:

>Translated_346_residues
MEADILDGKLKRVEVSKKGLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIELWKSDELSEDERRVILL
NLGFFSTAESLVGNNIVLAIFKHITNPEARQYLLRQAFEEAVHTHTFLYICESLGLDEGEVFNAYNERASIRAKDDFQMT
LTVDVLDPNFSVQSSEGLGQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDLING
IKEENPEVWTTELQEEIVALIEKAVELEIEYAKDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYHSRNPFPWMSE
TMDLNKEKNFFETRVTEYQTAGNLSW
>Mature_346_residues
MEADILDGKLKRVEVSKKGLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMARDIELWKSDELSEDERRVILL
NLGFFSTAESLVGNNIVLAIFKHITNPEARQYLLRQAFEEAVHTHTFLYICESLGLDEGEVFNAYNERASIRAKDDFQMT
LTVDVLDPNFSVQSSEGLGQFIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDLING
IKEENPEVWTTELQEEIVALIEKAVELEIEYAKDCLPRGILGLRSSMFIDYVRHIADRRLERIGLKPIYHSRNPFPWMSE
TMDLNKEKNFFETRVTEYQTAGNLSW

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Homo sapiens, GI42544136, Length=312, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI289177074, Length=312, Percent_Identity=26.9230769230769, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4557845, Length=318, Percent_Identity=27.0440251572327, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI260064013, Length=318, Percent_Identity=27.0440251572327, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI289177076, Length=288, Percent_Identity=26.7361111111111, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788567, Length=291, Percent_Identity=24.7422680412371, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551968, Length=315, Percent_Identity=27.3015873015873, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI193206379, Length=302, Percent_Identity=24.8344370860927, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17567153, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322434, Length=341, Percent_Identity=29.9120234604106, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321619, Length=318, Percent_Identity=22.9559748427673, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18079279, Length=318, Percent_Identity=27.6729559748428, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2_CHLPN (Q9Z6S4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72010
- PIR:   F86613
- RefSeq:   NP_225179.1
- RefSeq:   NP_301040.1
- RefSeq:   NP_445409.1
- RefSeq:   NP_877294.1
- ProteinModelPortal:   Q9Z6S4
- SMR:   Q9Z6S4
- GeneID:   1467701
- GeneID:   895607
- GeneID:   919749
- GeneID:   963323
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0871
- KEGG:   cpn:CPn0985
- KEGG:   cpt:CpB1022
- TIGR:   CP_0871
- HOGENOM:   HBG418082
- OMA:   FNMYREV
- PhylomeDB:   Q9Z6S4
- ProtClustDB:   PRK07209
- BioCyc:   CPNE115711:CP_0871-MONOMER
- BioCyc:   CPNE115713:CPN0985-MONOMER
- BioCyc:   CPNE138677:CPJ0985-MONOMER
- BioCyc:   CPNE182082:CPB1022-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 40200; Mature: 40200

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADILDGKLKRVEVSKKGLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMAR
CCCCCCCCCHHEEEECCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCC
DIELWKSDELSEDERRVILLNLGFFSTAESLVGNNIVLAIFKHITNPEARQYLLRQAFEE
CCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHH
AVHTHTFLYICESLGLDEGEVFNAYNERASIRAKDDFQMTLTVDVLDPNFSVQSSEGLGQ
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCCCCCCCHHH
FIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDLING
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECHHHHHC
IKEENPEVWTTELQEEIVALIEKAVELEIEYAKDCLPRGILGLRSSMFIDYVRHIADRRL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIGLKPIYHSRNPFPWMSETMDLNKEKNFFETRVTEYQTAGNLSW
HHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEADILDGKLKRVEVSKKGLVNCNQVDVNQLVPIKYKWAWEHYLNGCANNWLPTEVPMAR
CCCCCCCCCHHEEEECCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCC
DIELWKSDELSEDERRVILLNLGFFSTAESLVGNNIVLAIFKHITNPEARQYLLRQAFEE
CCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHH
AVHTHTFLYICESLGLDEGEVFNAYNERASIRAKDDFQMTLTVDVLDPNFSVQSSEGLGQ
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCCCCCCCHHH
FIKNLVGYYIIMEGIFFYSGFVMILSFHRQNKMTGIGEQYQYILRDETIHLNFGIDLING
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECHHHHHC
IKEENPEVWTTELQEEIVALIEKAVELEIEYAKDCLPRGILGLRSSMFIDYVRHIADRRL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIGLKPIYHSRNPFPWMSETMDLNKEKNFFETRVTEYQTAGNLSW
HHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362