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The map label for this gene is ribA_ribB

Identifier: 15836405

GI number: 15836405

Start: 989284

End: 990540

Strand: Direct

Name: ribA_ribB

Synonym: CPj0872

Alternate gene names: 15836405

Gene position: 989284-990540 (Clockwise)

Preceding gene: 15836404

Following gene: 15836406

Centisome position: 80.65

GC content: 42.8

Gene sequence:

>1257_bases
ATGATAGAAACTCGAGAAGAGGTAGGCTCTGCAAATTTTGTTTCTTTAGAACGAGCTATTGAGGATTTAAGAGCAGGAAA
ATTTGTTATTGTTGTTGATGAAGCTTCGAGAGAAGATGAAGGCGACCTGATTATCGCGGGAGAAAAAATTACAGTTGAAA
AGATGACGTTTCTTCTCCAGCACACCACAGGAGTGGTTTGCGCTGCTTTAAGCCAAGAACGTCTCTTAAGCTTGGATCTT
CCTCCCATGGTTAAGGATAACCGTTGCCGTTTTAAAACTCCCTTCACTGTATCCGTAGATGCTGCTCACGGGGTGACTAC
AGGAGTTTCTGCCGCAGATAGAACCAAAGTCGTTCAGTTATTAGCAGATCCTAAGAGCAAACCCGAAGATTTTATTAGCC
CAGGACACTTTTTCCCCCTAGCAAGTTCTCCAGGAGGAGTGTTAAAACGAGCAGGTCATACAGAATCTACTGTTGACCTA
ATGGAGTTGGCAGGACTGCAGCCTTGTGGTGTACTCGCAGAATTAGTGAACGAAGACTACTCTATGATGCGATTGCCTCA
AATTTTAGAGTTCGCAAGGAAACATAATATTGCAGTGATTCCCGTGACATCGATCATTGCTCATCGCATGCTCTCCGATC
GTTTGGTTTCTAAAATCTCTTCAGCACGCCTCCCTACAATTTACGGAGACTTTACGATTCATGTCTATGAATCCTTATTG
GAAGGAATGCAACATCTTGCTTTGGTAAAAGGCAATGTTGCTGGAAAAAGTAATGTCCTCGTACGTGTCCACTCAGAGTG
TGTCACAGGAGATATTTTAGGATCTAAGCGCTGTGATTGTGGAGAACAATTGAGTTCAGCAATGTCCTACATTGCTGAGA
AGGGAACTGGTGTTCTTGTTTACTTGCGAGGGCAGGAAGGCCGAGGGATCGGTTTGGGCCATAAAGTACGTGCTTATGCT
TTGCAAGATAACGGTTATGATACTGTAGATGCAAACTTAGCTATGGGATTTCCCGTAGACTCAAGGGAATATGGCATAGG
AGCACAAATTCTTGTTGATCTCAAGTTGACAACCATAAAATTAATCACTCATAATCCTCAAAAATATTTTGGGCTTCAAG
GTTTCGGACTTAGCATCACAGAAAGAGTTCCTCTTCCTGTTCGCATTTCTGAAGACAATGAGCAGTATTTAAGAACAAAA
CAGGAACGTATGGGACATTGGCTAGATCTCCCATGCTGTAACAATCGGGTACAATAA

Upstream 100 bases:

>100_bases
GGAGATTTCCCCTCAGGTTTTCGAGCCCATAAGGAATTGAAAGGAAAGGATTTTTAGAGTAGCATGCTTAGCATCGGAAT
CCGTTAATGCGAGGGAGTCA

Downstream 100 bases:

>100_bases
TTTTGAGGAGTATATGAAAACATTGAAAGGACATTTGTCTGCAAAGAATCTACGTATTGCTATTGTCGGCTCCTGCTTTA
ATCAAGCTATGGCTGATGCC

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIETREEVGSANFVSLERAIEDLRAGKFVIVVDEASREDEGDLIIAGEKITVEKMTFLLQHTTGVVCAALSQERLLSLDL
PPMVKDNRCRFKTPFTVSVDAAHGVTTGVSAADRTKVVQLLADPKSKPEDFISPGHFFPLASSPGGVLKRAGHTESTVDL
MELAGLQPCGVLAELVNEDYSMMRLPQILEFARKHNIAVIPVTSIIAHRMLSDRLVSKISSARLPTIYGDFTIHVYESLL
EGMQHLALVKGNVAGKSNVLVRVHSECVTGDILGSKRCDCGEQLSSAMSYIAEKGTGVLVYLRGQEGRGIGLGHKVRAYA
LQDNGYDTVDANLAMGFPVDSREYGIGAQILVDLKLTTIKLITHNPQKYFGLQGFGLSITERVPLPVRISEDNEQYLRTK
QERMGHWLDLPCCNNRVQ

Sequences:

>Translated_418_residues
MIETREEVGSANFVSLERAIEDLRAGKFVIVVDEASREDEGDLIIAGEKITVEKMTFLLQHTTGVVCAALSQERLLSLDL
PPMVKDNRCRFKTPFTVSVDAAHGVTTGVSAADRTKVVQLLADPKSKPEDFISPGHFFPLASSPGGVLKRAGHTESTVDL
MELAGLQPCGVLAELVNEDYSMMRLPQILEFARKHNIAVIPVTSIIAHRMLSDRLVSKISSARLPTIYGDFTIHVYESLL
EGMQHLALVKGNVAGKSNVLVRVHSECVTGDILGSKRCDCGEQLSSAMSYIAEKGTGVLVYLRGQEGRGIGLGHKVRAYA
LQDNGYDTVDANLAMGFPVDSREYGIGAQILVDLKLTTIKLITHNPQKYFGLQGFGLSITERVPLPVRISEDNEQYLRTK
QERMGHWLDLPCCNNRVQ
>Mature_418_residues
MIETREEVGSANFVSLERAIEDLRAGKFVIVVDEASREDEGDLIIAGEKITVEKMTFLLQHTTGVVCAALSQERLLSLDL
PPMVKDNRCRFKTPFTVSVDAAHGVTTGVSAADRTKVVQLLADPKSKPEDFISPGHFFPLASSPGGVLKRAGHTESTVDL
MELAGLQPCGVLAELVNEDYSMMRLPQILEFARKHNIAVIPVTSIIAHRMLSDRLVSKISSARLPTIYGDFTIHVYESLL
EGMQHLALVKGNVAGKSNVLVRVHSECVTGDILGSKRCDCGEQLSSAMSYIAEKGTGVLVYLRGQEGRGIGLGHKVRAYA
LQDNGYDTVDANLAMGFPVDSREYGIGAQILVDLKLTTIKLITHNPQKYFGLQGFGLSITERVPLPVRISEDNEQYLRTK
QERMGHWLDLPCCNNRVQ

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=205, Percent_Identity=47.3170731707317, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1787533, Length=194, Percent_Identity=50.5154639175258, Blast_Score=197, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=44.2786069651741, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319438, Length=176, Percent_Identity=47.1590909090909, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_CHLPN (Q9Z734)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   F86599
- PIR:   H72026
- RefSeq:   NP_225067.1
- RefSeq:   NP_300929.1
- RefSeq:   NP_445534.1
- RefSeq:   NP_877173.1
- ProteinModelPortal:   Q9Z734
- GeneID:   1467580
- GeneID:   895694
- GeneID:   919635
- GeneID:   963470
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0997
- KEGG:   cpn:CPn0872
- KEGG:   cpt:CpB0901
- TIGR:   CP_0997
- HOGENOM:   HBG735778
- OMA:   RCDCRMQ
- PhylomeDB:   Q9Z734
- ProtClustDB:   PRK09311
- BioCyc:   CPNE115711:CP_0997-MONOMER
- BioCyc:   CPNE115713:CPN0872-MONOMER
- BioCyc:   CPNE138677:CPJ0872-MONOMER
- BioCyc:   CPNE182082:CPB0901-MONOMER
- BRENDA:   3.5.4.25
- BRENDA:   4.1.99.12
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 45846; Mature: 45846

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 340-340 ACT_SITE 342-342 BINDING 42-42 BINDING 174-174 BINDING 283-283 BINDING 328-328 BINDING 363-363 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETREEVGSANFVSLERAIEDLRAGKFVIVVDEASREDEGDLIIAGEKITVEKMTFLLQ
CCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCEEEHHHHHHHHH
HTTGVVCAALSQERLLSLDLPPMVKDNRCRFKTPFTVSVDAAHGVTTGVSAADRTKVVQL
HHCCHHEEHHCHHHEEECCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHH
LADPKSKPEDFISPGHFFPLASSPGGVLKRAGHTESTVDLMELAGLQPCGVLAELVNEDY
HHCCCCCCHHHCCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCH
SMMRLPQILEFARKHNIAVIPVTSIIAHRMLSDRLVSKISSARLPTIYGDFTIHVYESLL
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
EGMQHLALVKGNVAGKSNVLVRVHSECVTGDILGSKRCDCGEQLSSAMSYIAEKGTGVLV
HHHHHHHHHCCCCCCCCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
YLRGQEGRGIGLGHKVRAYALQDNGYDTVDANLAMGFPVDSREYGIGAQILVDLKLTTIK
EEECCCCCCCCCCCEEEEEEEECCCCCEECCCEEECCCCCCCCCCCCEEEEEEEEEEEEE
LITHNPQKYFGLQGFGLSITERVPLPVRISEDNEQYLRTKQERMGHWLDLPCCNNRVQ
EEECCCHHHCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure
MIETREEVGSANFVSLERAIEDLRAGKFVIVVDEASREDEGDLIIAGEKITVEKMTFLLQ
CCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCEEEHHHHHHHHH
HTTGVVCAALSQERLLSLDLPPMVKDNRCRFKTPFTVSVDAAHGVTTGVSAADRTKVVQL
HHCCHHEEHHCHHHEEECCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHH
LADPKSKPEDFISPGHFFPLASSPGGVLKRAGHTESTVDLMELAGLQPCGVLAELVNEDY
HHCCCCCCHHHCCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCH
SMMRLPQILEFARKHNIAVIPVTSIIAHRMLSDRLVSKISSARLPTIYGDFTIHVYESLL
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
EGMQHLALVKGNVAGKSNVLVRVHSECVTGDILGSKRCDCGEQLSSAMSYIAEKGTGVLV
HHHHHHHHHCCCCCCCCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
YLRGQEGRGIGLGHKVRAYALQDNGYDTVDANLAMGFPVDSREYGIGAQILVDLKLTTIK
EEECCCCCCCCCCCEEEEEEEECCCCCEECCCEEECCCCCCCCCCCCEEEEEEEEEEEEE
LITHNPQKYFGLQGFGLSITERVPLPVRISEDNEQYLRTKQERMGHWLDLPCCNNRVQ
EEECCCHHHCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362