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The map label for this gene is ribH

Identifier: 15836406

GI number: 15836406

Start: 990554

End: 991018

Strand: Direct

Name: ribH

Synonym: CPj0873

Alternate gene names: 15836406

Gene position: 990554-991018 (Clockwise)

Preceding gene: 15836405

Following gene: 15836408

Centisome position: 80.76

GC content: 41.94

Gene sequence:

>465_bases
ATGAAAACATTGAAAGGACATTTGTCTGCAAAGAATCTACGTATTGCTATTGTCGGCTCCTGCTTTAATCAAGCTATGGC
TGATGCCCTAGTTTCTGGTACTCAGGAAACTTTTTTGAAGTTTGGAGGGAGCGAGGACGGCTTGATGACTATCCGTGTTC
CCGGAGCTTTTGAGATTCCCTGTACGATCAAAAAACTTTTATCTTCTGAAAGAAAGTTCGATGCTATTGTTGCATGCGGC
GTCCTAATTCAAGGAGAAACAGACCATTATAACCAAATTGTAAATCAAGTAGCGGCAGGTATTGGTGCTCTCTCTTTGGA
ATTTTGTCTTCCCATAACCTTGTCCATAGTTGCAGCTCCTTCTGCAGAAATCGCTTGGCAAAGATCAGGTATTAAAGGAC
GTCATTTGGGAGTTTCTGGGATGACGACAGCTATAGAAATGGCAACGTTATTCACTCAAATCTAG

Upstream 100 bases:

>100_bases
GAAGACAATGAGCAGTATTTAAGAACAAAACAGGAACGTATGGGACATTGGCTAGATCTCCCATGCTGTAACAATCGGGT
ACAATAATTTTGAGGAGTAT

Downstream 100 bases:

>100_bases
TTCTTGTAAGAACGTATACGTCCCACAAAATTTGTGGGACTCTTCCTTCTACAGATTGATGCTGACAGCTCCATTTAAAT
GGATGCGAACTGCGATACTC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKTLKGHLSAKNLRIAIVGSCFNQAMADALVSGTQETFLKFGGSEDGLMTIRVPGAFEIPCTIKKLLSSERKFDAIVACG
VLIQGETDHYNQIVNQVAAGIGALSLEFCLPITLSIVAAPSAEIAWQRSGIKGRHLGVSGMTTAIEMATLFTQI

Sequences:

>Translated_154_residues
MKTLKGHLSAKNLRIAIVGSCFNQAMADALVSGTQETFLKFGGSEDGLMTIRVPGAFEIPCTIKKLLSSERKFDAIVACG
VLIQGETDHYNQIVNQVAAGIGALSLEFCLPITLSIVAAPSAEIAWQRSGIKGRHLGVSGMTTAIEMATLFTQI
>Mature_154_residues
MKTLKGHLSAKNLRIAIVGSCFNQAMADALVSGTQETFLKFGGSEDGLMTIRVPGAFEIPCTIKKLLSSERKFDAIVACG
VLIQGETDHYNQIVNQVAAGIGALSLEFCLPITLSIVAAPSAEIAWQRSGIKGRHLGVSGMTTAIEMATLFTQI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=27.0967741935484, Blast_Score=75, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324429, Length=147, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CHLPN (Q9Z733)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72027
- PIR:   G86599
- RefSeq:   NP_225068.1
- RefSeq:   NP_300930.1
- RefSeq:   NP_445533.1
- RefSeq:   NP_877174.1
- ProteinModelPortal:   Q9Z733
- SMR:   Q9Z733
- PHCI-2DPAGE:   Q9Z733
- GeneID:   1467581
- GeneID:   895431
- GeneID:   919636
- GeneID:   963468
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0996
- KEGG:   cpn:CPn0873
- KEGG:   cpt:CpB0902
- TIGR:   CP_0996
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   CPNE115711:CP_0996-MONOMER
- BioCyc:   CPNE115713:CPN0873-MONOMER
- BioCyc:   CPNE138677:CPJ0873-MONOMER
- BioCyc:   CPNE182082:CPB0902-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16340; Mature: 16340

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLKGHLSAKNLRIAIVGSCFNQAMADALVSGTQETFLKFGGSEDGLMTIRVPGAFEIP
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCEECC
CTIKKLLSSERKFDAIVACGVLIQGETDHYNQIVNQVAAGIGALSLEFCLPITLSIVAAP
HHHHHHHCCCCCHHHHEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECC
SAEIAWQRSGIKGRHLGVSGMTTAIEMATLFTQI
CCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLKGHLSAKNLRIAIVGSCFNQAMADALVSGTQETFLKFGGSEDGLMTIRVPGAFEIP
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCEECC
CTIKKLLSSERKFDAIVACGVLIQGETDHYNQIVNQVAAGIGALSLEFCLPITLSIVAAP
HHHHHHHCCCCCHHHHEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECC
SAEIAWQRSGIKGRHLGVSGMTTAIEMATLFTQI
CCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362