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The map label for this gene is glgX

Identifier: 15835921

GI number: 15835921

Start: 435038

End: 437032

Strand: Direct

Name: glgX

Synonym: CPj0388

Alternate gene names: 15835921

Gene position: 435038-437032 (Clockwise)

Preceding gene: 15835913

Following gene: 15835925

Centisome position: 35.47

GC content: 43.16

Gene sequence:

>1995_bases
ATGGAAAAAGTTTCTTCTTATCCCTCAGTTCCTTTACCTCTTGGGGCTTCTAAAATTTCCCCAAACCGCTATCGATTTGC
TTTATATGCTTCACAAGCTACCGAAGTCATCCTTGCTTTAACAGACGAAAATTCAGAAGTCATAGAAGTCCCTCTTTACC
CCGATACACACCGCACGGGTGCGATTTGGCATATAGAGATCGAGGGTATTTCTGATCAATCGTCTTATGCATTTCGTGTT
CATGGGCCTAAAAAGCATGGAATGCAATACTCTTTTAAAGAATATCTTGCAGATCCCTATGCGAAGAATATTCATTCCCC
ACAGAGTTTTGGTTCGCGAAAGAAACAGGGGGATTATGCATTTTGTTATTTAAAGGAAGAACCATTTCCTTGGGATGGTG
ATCAGCCTCTGCATTTGCCGAAAGAAGAGATGATCATCTATGAGATGCATGTACGTTCCTTCACGCAATCTTCTTCATCT
AGGGTTCATGCTCCGGGAACCTTCCTAGGAATCATTGAAAAGATCGACCATCTGCATAAGCTGGGAATCAACGCTGTTGA
ACTCTTACCTATCTTTGAGTTCGATGAGACTGCGCATCCTTTTAGAAATTCGAAATTCCCTTATCTGTGCAATTATTGGG
GTTATGCTCCCCTAAATTTCTTTTCTCCTTGCCGACGTTATGCTTATGCCTCTGATCCTTGCGCTCCAAGTAGAGAGTTT
AAAACTTTAGTAAAGACCTTGCATCAAGAAGGTATTGAGGTCATTCTTGATGTTGTTTTTAATCATACGGGCTTGCAAGG
GACGACCTGCTCTTTGCCTTGGATAGACACTCCGAGCTATTATATTTTAGATGCACAAGGTCACTTTACAAATTATTCAG
GCTGTGGAAACACTCTCAATACAAACCGCGCCCCCACGACCCAATGGATTCTCGACATCTTACGTTATTGGGTAGAAGAA
ATGCATGTCGATGGGTTCCGATTTGATCTTGCTTCTGTCTTTTCTCGTGGTCCTTCGGGATCTCCCCTACAATTCGCTCC
TGTTTTAGAGGCGATTTCTTTTGATCCTTTACTTGCGAGCACAAAGATTATAGCTGAGCCTTGGGATGCTGGCGGTTTGT
ATCAGGTGGGCTATTTCCCCACACTGTCTCCAAGATGGAGTGAATGGAACGGTCCGTATCGTGATAACGTGAAAGCATTT
CTTAATGGGGATCAAAATCTCATAGGAACCTTTGCTTCTAGAATTTCAGGATCTCAAGACATCTATCCTCACGGCTCGCC
TACAAATTCGATTAACTATGTCAGTTGCCATGATGGTTTTACGTTATGTGACACTGTGACTTATAACCACAAACATAATG
AGGCTAACGGAGAGGATAATCGTGACGGCACAGATGCGAACTACAGCTACAATTTCGGAACGGAAGGGAAAACAGAAGAC
CCTGGCATTCTTGAAGTTCGTGAAAGACAGTTACGAAATTTTTTCCTTACTTTGATGGTCTCGCAAGGCATTCCGATGAT
TCAATCAGGAGATGAGTATGCCCATACCGCGGAAGGCAATAACAACCGTTGGGCTTTGGATTCGAATGCGAATTACTTCC
TTTGGGATCAGCTTACCGCAAAGCCTACACTGATGCACTTTCTCTGTGATCTCATTGCGTTTCGAAAAAAATATAAAACA
CTTTTTAATCGAGGCTTTCTTTCCAATAAGGAAATCAGTTGGGTAGATGCTATGGGAAATCCCATGACATGGCGCCCTGG
AAATTTCTTAGCATTTAAAATAAAATCGCCAAAAGCGCATGTATATGTTGCTTTTCACGTGGGAGCTCAAGACCAACTTG
CGACCTTACCTAAAGCCTCCAGCAACTTTCTTCCTTATCAAATAGTTGCCGAGAGTCAGCAAGGGTTTGTCCCTCAAAAT
GTAGCAACGCCGACAGTGTCGCTACAGCCCCATACCACGCTAATTGCGATCAGCCATGCGAAAGAGGTTACCTGA

Upstream 100 bases:

>100_bases
TTCAAGTTACCATAAGAGTGATTTGTTAGTAAACGATTTCAGAGGCTTAAAATCAAAAACTCCCTTGTATTTCTGGCGCC
GTTATGATATCTGATCGACG

Downstream 100 bases:

>100_bases
TCTCTCCGTCCAGTTCTTCATTCCAGGATTCTATAACTACAAAATCCACATCCTTGTAGAACTTCTCAAGAATCTGACGT
GCATTGAATCCTTCTATAGC

Product: glycogen hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MEKVSSYPSVPLPLGASKISPNRYRFALYASQATEVILALTDENSEVIEVPLYPDTHRTGAIWHIEIEGISDQSSYAFRV
HGPKKHGMQYSFKEYLADPYAKNIHSPQSFGSRKKQGDYAFCYLKEEPFPWDGDQPLHLPKEEMIIYEMHVRSFTQSSSS
RVHAPGTFLGIIEKIDHLHKLGINAVELLPIFEFDETAHPFRNSKFPYLCNYWGYAPLNFFSPCRRYAYASDPCAPSREF
KTLVKTLHQEGIEVILDVVFNHTGLQGTTCSLPWIDTPSYYILDAQGHFTNYSGCGNTLNTNRAPTTQWILDILRYWVEE
MHVDGFRFDLASVFSRGPSGSPLQFAPVLEAISFDPLLASTKIIAEPWDAGGLYQVGYFPTLSPRWSEWNGPYRDNVKAF
LNGDQNLIGTFASRISGSQDIYPHGSPTNSINYVSCHDGFTLCDTVTYNHKHNEANGEDNRDGTDANYSYNFGTEGKTED
PGILEVRERQLRNFFLTLMVSQGIPMIQSGDEYAHTAEGNNNRWALDSNANYFLWDQLTAKPTLMHFLCDLIAFRKKYKT
LFNRGFLSNKEISWVDAMGNPMTWRPGNFLAFKIKSPKAHVYVAFHVGAQDQLATLPKASSNFLPYQIVAESQQGFVPQN
VATPTVSLQPHTTLIAISHAKEVT

Sequences:

>Translated_664_residues
MEKVSSYPSVPLPLGASKISPNRYRFALYASQATEVILALTDENSEVIEVPLYPDTHRTGAIWHIEIEGISDQSSYAFRV
HGPKKHGMQYSFKEYLADPYAKNIHSPQSFGSRKKQGDYAFCYLKEEPFPWDGDQPLHLPKEEMIIYEMHVRSFTQSSSS
RVHAPGTFLGIIEKIDHLHKLGINAVELLPIFEFDETAHPFRNSKFPYLCNYWGYAPLNFFSPCRRYAYASDPCAPSREF
KTLVKTLHQEGIEVILDVVFNHTGLQGTTCSLPWIDTPSYYILDAQGHFTNYSGCGNTLNTNRAPTTQWILDILRYWVEE
MHVDGFRFDLASVFSRGPSGSPLQFAPVLEAISFDPLLASTKIIAEPWDAGGLYQVGYFPTLSPRWSEWNGPYRDNVKAF
LNGDQNLIGTFASRISGSQDIYPHGSPTNSINYVSCHDGFTLCDTVTYNHKHNEANGEDNRDGTDANYSYNFGTEGKTED
PGILEVRERQLRNFFLTLMVSQGIPMIQSGDEYAHTAEGNNNRWALDSNANYFLWDQLTAKPTLMHFLCDLIAFRKKYKT
LFNRGFLSNKEISWVDAMGNPMTWRPGNFLAFKIKSPKAHVYVAFHVGAQDQLATLPKASSNFLPYQIVAESQQGFVPQN
VATPTVSLQPHTTLIAISHAKEVT
>Mature_664_residues
MEKVSSYPSVPLPLGASKISPNRYRFALYASQATEVILALTDENSEVIEVPLYPDTHRTGAIWHIEIEGISDQSSYAFRV
HGPKKHGMQYSFKEYLADPYAKNIHSPQSFGSRKKQGDYAFCYLKEEPFPWDGDQPLHLPKEEMIIYEMHVRSFTQSSSS
RVHAPGTFLGIIEKIDHLHKLGINAVELLPIFEFDETAHPFRNSKFPYLCNYWGYAPLNFFSPCRRYAYASDPCAPSREF
KTLVKTLHQEGIEVILDVVFNHTGLQGTTCSLPWIDTPSYYILDAQGHFTNYSGCGNTLNTNRAPTTQWILDILRYWVEE
MHVDGFRFDLASVFSRGPSGSPLQFAPVLEAISFDPLLASTKIIAEPWDAGGLYQVGYFPTLSPRWSEWNGPYRDNVKAF
LNGDQNLIGTFASRISGSQDIYPHGSPTNSINYVSCHDGFTLCDTVTYNHKHNEANGEDNRDGTDANYSYNFGTEGKTED
PGILEVRERQLRNFFLTLMVSQGIPMIQSGDEYAHTAEGNNNRWALDSNANYFLWDQLTAKPTLMHFLCDLIAFRKKYKT
LFNRGFLSNKEISWVDAMGNPMTWRPGNFLAFKIKSPKAHVYVAFHVGAQDQLATLPKASSNFLPYQIVAESQQGFVPQN
VATPTVSLQPHTTLIAISHAKEVT

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=604, Percent_Identity=37.2516556291391, Blast_Score=343, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75031; Mature: 75031

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVSSYPSVPLPLGASKISPNRYRFALYASQATEVILALTDENSEVIEVPLYPDTHRTG
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCC
AIWHIEIEGISDQSSYAFRVHGPKKHGMQYSFKEYLADPYAKNIHSPQSFGSRKKQGDYA
CEEEEEEECCCCCCCEEEEEECCHHCCCCCHHHHHHCCHHHHCCCCCHHHCCCCCCCCEE
FCYLKEEPFPWDGDQPLHLPKEEMIIYEMHVRSFTQSSSSRVHAPGTFLGIIEKIDHLHK
EEEEECCCCCCCCCCCCCCCHHHEEEEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
LGINAVELLPIFEFDETAHPFRNSKFPYLCNYWGYAPLNFFSPCRRYAYASDPCAPSREF
CCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
KTLVKTLHQEGIEVILDVVFNHTGLQGTTCSLPWIDTPSYYILDAQGHFTNYSGCGNTLN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
TNRAPTTQWILDILRYWVEEMHVDGFRFDLASVFSRGPSGSPLQFAPVLEAISFDPLLAS
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCHHHC
TKIIAEPWDAGGLYQVGYFPTLSPRWSEWNGPYRDNVKAFLNGDQNLIGTFASRISGSQD
CEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCC
IYPHGSPTNSINYVSCHDGFTLCDTVTYNHKHNEANGEDNRDGTDANYSYNFGTEGKTED
CCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
PGILEVRERQLRNFFLTLMVSQGIPMIQSGDEYAHTAEGNNNRWALDSNANYFLWDQLTA
CCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCEEEECCCCCEEEEECCCC
KPTLMHFLCDLIAFRKKYKTLFNRGFLSNKEISWVDAMGNPMTWRPGNFLAFKIKSPKAH
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCEEEEEEECCCEE
VYVAFHVGAQDQLATLPKASSNFLPYQIVAESQQGFVPQNVATPTVSLQPHTTLIAISHA
EEEEEEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECC
KEVT
CCCC
>Mature Secondary Structure
MEKVSSYPSVPLPLGASKISPNRYRFALYASQATEVILALTDENSEVIEVPLYPDTHRTG
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCC
AIWHIEIEGISDQSSYAFRVHGPKKHGMQYSFKEYLADPYAKNIHSPQSFGSRKKQGDYA
CEEEEEEECCCCCCCEEEEEECCHHCCCCCHHHHHHCCHHHHCCCCCHHHCCCCCCCCEE
FCYLKEEPFPWDGDQPLHLPKEEMIIYEMHVRSFTQSSSSRVHAPGTFLGIIEKIDHLHK
EEEEECCCCCCCCCCCCCCCHHHEEEEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
LGINAVELLPIFEFDETAHPFRNSKFPYLCNYWGYAPLNFFSPCRRYAYASDPCAPSREF
CCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
KTLVKTLHQEGIEVILDVVFNHTGLQGTTCSLPWIDTPSYYILDAQGHFTNYSGCGNTLN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
TNRAPTTQWILDILRYWVEEMHVDGFRFDLASVFSRGPSGSPLQFAPVLEAISFDPLLAS
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCHHHC
TKIIAEPWDAGGLYQVGYFPTLSPRWSEWNGPYRDNVKAFLNGDQNLIGTFASRISGSQD
CEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCC
IYPHGSPTNSINYVSCHDGFTLCDTVTYNHKHNEANGEDNRDGTDANYSYNFGTEGKTED
CCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
PGILEVRERQLRNFFLTLMVSQGIPMIQSGDEYAHTAEGNNNRWALDSNANYFLWDQLTA
CCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCEEEECCCCCEEEEECCCC
KPTLMHFLCDLIAFRKKYKTLFNRGFLSNKEISWVDAMGNPMTWRPGNFLAFKIKSPKAH
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCEEEEEEECCCEE
VYVAFHVGAQDQLATLPKASSNFLPYQIVAESQQGFVPQNVATPTVSLQPHTTLIAISHA
EEEEEEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECC
KEVT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]