Click here to switch to the map view.

The map label for this gene is pepA

Identifier: 15835918

GI number: 15835918

Start: 432228

End: 433727

Strand: Reverse

Name: pepA

Synonym: CPj0385

Alternate gene names: 15835918

Gene position: 433727-432228 (Counterclockwise)

Preceding gene: 15835919

Following gene: 15835917

Centisome position: 35.36

GC content: 41.93

Gene sequence:

>1500_bases
GTGGTTTTATTTCATGCTCAAGCCTCTGGGCGTAATCGTGTTAAGGCAGATGCTATAGTCCTGCCCTTTTGGCATTTTAA
GGATGCAAAAAATGCAGCTTCTTTTGAAGCCGAGTTTGAACCCTCGTATCTCCCCGCTTTAGAAAACTTTCAAGGAAAAA
CCGGGGAGATTGAACTCCTTTATAGTAGTCCTAAAGCTAAGGAAAAACGCATTGTCCTCTTAGGCTTAGGGAAAAATGAA
GAGCTCACCTCTGATGTTGTTTTCCAAACCTATGCGACACTAACTCGTGTCTTACGTAAAGCAAAGTGTTCCACAGTCAA
TATCATCTTACCTACAATTTCTGAATTGCGGCTTTCTGCCGAAGAATTCTTAGTGGGGTTGTCCTCAGGAATTTTGTCAT
TAAACTATGACTACCCACGTTATAATAAGGTAGATCGTAATCTTGAAACTCCTCTTTCTAAAGTCACGGTTATCGGTATC
GTTCCCAAAATGGCGGATGCTATCTTTAGGAAAGAAGCAGCCATTTTCGAAGGCGTATATCTCACTCGAGATCTTGTGAA
CAGGAATGCTGATGAAATTACCCCTAAGAAATTGGCAGAGGTTGCTCTGAATCTGGGAAAAGAGTTCCCTAGTATTGATA
CTAAGGTCTTGGGAAAAGATGCCATCGCCAAAGAGAAAATGGGACTCCTATTGGCTGTTTCCAAGGGTTCTTGTGTGGAT
CCACACTTTATCGTTGTCCGTTATCAAGGACGTCCTAAGTCTAAAGATCACACCGTCTTGATAGGGAAAGGGGTCACTTT
TGACTCTGGAGGTTTAGACCTCAAGCCTGGAAAATCCATGCTTACTATGAAAGAAGACATGGCAGGTGGGGCTACAGTCC
TCGGGATTCTCTCGGCGTTAGCAGTTTTAGAGCTTCCTATAAATGTCACGGGGATCATTCCTGCTACAGAGAATGCTATC
GATGGCGCCTCCTATAAAATGGGAGATGTCTATGTAGGAATGTCGGGGCTTTCTGTTGAGATTTGTAGTACCGATGCTGA
GGGACGTCTTATCCTCGCTGATGCGATTACATATGCTTTAAAATATTGTAAACCGACACGTATTATAGATTTTGCAACTC
TAACAGGAGCTATGGTAGTCTCTCTAGGAGAAGAGGTTGCAGGTTTCTTTTCCAATAACGATGTTTTAGCTGAAGATCTT
TTAGAGGCGTCAGCCGAAACCTCCGAGCCGTTATGGAGACTTCCTCTAGTTAAGAAGTATGATAAAACATTGCATTCTGA
TATTGCTGATATGAAAAATCTAGGCAGTAACCGTGCAGGGGCTATTACAGCAGCATTATTCTTGCAGAGATTTTTGGAAG
AATCTTCGGTAGCTTGGGCACATCTTGATATTGCAGGTACTGCATATCATGAAAAAGAAGAAGACCGTTATCCAAAATAT
GCTTCAGGTTTTGGTGTTCGTTCTATTCTTTATTACTTAGAAAATAGTCTTTCTAAGTAG

Upstream 100 bases:

>100_bases
AGCTATTAAAGATAAAGATATGTATGCTGGTTATGGTCAAGAACAGCAGTATGTCTGTGAAGATGTTCCTTTTTAATTCC
TAGTCATTAAAGGAGAGTTT

Downstream 100 bases:

>100_bases
TTGCTTTCTATTTATTTATGTTTTAGTAATGACTTTTATTTTAGTTTTTTTAAAATAAAAGTCATTTTTTTTATTAAAGT
TTTCAATCGTCCCTGCCGAT

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MVLFHAQASGRNRVKADAIVLPFWHFKDAKNAASFEAEFEPSYLPALENFQGKTGEIELLYSSPKAKEKRIVLLGLGKNE
ELTSDVVFQTYATLTRVLRKAKCSTVNIILPTISELRLSAEEFLVGLSSGILSLNYDYPRYNKVDRNLETPLSKVTVIGI
VPKMADAIFRKEAAIFEGVYLTRDLVNRNADEITPKKLAEVALNLGKEFPSIDTKVLGKDAIAKEKMGLLLAVSKGSCVD
PHFIVVRYQGRPKSKDHTVLIGKGVTFDSGGLDLKPGKSMLTMKEDMAGGATVLGILSALAVLELPINVTGIIPATENAI
DGASYKMGDVYVGMSGLSVEICSTDAEGRLILADAITYALKYCKPTRIIDFATLTGAMVVSLGEEVAGFFSNNDVLAEDL
LEASAETSEPLWRLPLVKKYDKTLHSDIADMKNLGSNRAGAITAALFLQRFLEESSVAWAHLDIAGTAYHEKEEDRYPKY
ASGFGVRSILYYLENSLSK

Sequences:

>Translated_499_residues
MVLFHAQASGRNRVKADAIVLPFWHFKDAKNAASFEAEFEPSYLPALENFQGKTGEIELLYSSPKAKEKRIVLLGLGKNE
ELTSDVVFQTYATLTRVLRKAKCSTVNIILPTISELRLSAEEFLVGLSSGILSLNYDYPRYNKVDRNLETPLSKVTVIGI
VPKMADAIFRKEAAIFEGVYLTRDLVNRNADEITPKKLAEVALNLGKEFPSIDTKVLGKDAIAKEKMGLLLAVSKGSCVD
PHFIVVRYQGRPKSKDHTVLIGKGVTFDSGGLDLKPGKSMLTMKEDMAGGATVLGILSALAVLELPINVTGIIPATENAI
DGASYKMGDVYVGMSGLSVEICSTDAEGRLILADAITYALKYCKPTRIIDFATLTGAMVVSLGEEVAGFFSNNDVLAEDL
LEASAETSEPLWRLPLVKKYDKTLHSDIADMKNLGSNRAGAITAALFLQRFLEESSVAWAHLDIAGTAYHEKEEDRYPKY
ASGFGVRSILYYLENSLSK
>Mature_499_residues
MVLFHAQASGRNRVKADAIVLPFWHFKDAKNAASFEAEFEPSYLPALENFQGKTGEIELLYSSPKAKEKRIVLLGLGKNE
ELTSDVVFQTYATLTRVLRKAKCSTVNIILPTISELRLSAEEFLVGLSSGILSLNYDYPRYNKVDRNLETPLSKVTVIGI
VPKMADAIFRKEAAIFEGVYLTRDLVNRNADEITPKKLAEVALNLGKEFPSIDTKVLGKDAIAKEKMGLLLAVSKGSCVD
PHFIVVRYQGRPKSKDHTVLIGKGVTFDSGGLDLKPGKSMLTMKEDMAGGATVLGILSALAVLELPINVTGIIPATENAI
DGASYKMGDVYVGMSGLSVEICSTDAEGRLILADAITYALKYCKPTRIIDFATLTGAMVVSLGEEVAGFFSNNDVLAEDL
LEASAETSEPLWRLPLVKKYDKTLHSDIADMKNLGSNRAGAITAALFLQRFLEESSVAWAHLDIAGTAYHEKEEDRYPKY
ASGFGVRSILYYLENSLSK

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family

Homologues:

Organism=Homo sapiens, GI41393561, Length=329, Percent_Identity=39.209726443769, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI47155554, Length=323, Percent_Identity=30.9597523219814, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1790710, Length=428, Percent_Identity=34.5794392523364, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI87082123, Length=316, Percent_Identity=38.9240506329114, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17556903, Length=310, Percent_Identity=33.8709677419355, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565172, Length=211, Percent_Identity=33.6492890995261, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24661038, Length=323, Percent_Identity=34.3653250773994, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21355725, Length=323, Percent_Identity=34.0557275541796, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI20129969, Length=308, Percent_Identity=32.1428571428571, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24662227, Length=332, Percent_Identity=29.5180722891566, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI161077148, Length=334, Percent_Identity=29.940119760479, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI20130057, Length=334, Percent_Identity=29.940119760479, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355645, Length=304, Percent_Identity=30.2631578947368, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24662223, Length=304, Percent_Identity=30.2631578947368, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI20129963, Length=289, Percent_Identity=31.4878892733564, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922386, Length=324, Percent_Identity=30.2469135802469, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI21357381, Length=321, Percent_Identity=31.4641744548287, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221379063, Length=321, Percent_Identity=31.4641744548287, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI221379062, Length=321, Percent_Identity=31.4641744548287, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646701, Length=352, Percent_Identity=26.4204545454545, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24646703, Length=352, Percent_Identity=26.4204545454545, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21358201, Length=352, Percent_Identity=26.4204545454545, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPA_CHLPN (Q9Z8F8)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   G72083
- PIR:   G86538
- RefSeq:   NP_224585.1
- RefSeq:   NP_300442.1
- RefSeq:   NP_444918.1
- RefSeq:   NP_876671.1
- ProteinModelPortal:   Q9Z8F8
- SMR:   Q9Z8F8
- PHCI-2DPAGE:   Q9Z8F8
- GeneID:   1467078
- GeneID:   894916
- GeneID:   919228
- GeneID:   963846
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0370
- KEGG:   cpn:CPn0385
- KEGG:   cpt:CpB0397
- TIGR:   CP_0370
- HOGENOM:   HBG742580
- OMA:   CKPTRII
- PhylomeDB:   Q9Z8F8
- ProtClustDB:   PRK00913
- BioCyc:   CPNE115711:CP_0370-MONOMER
- BioCyc:   CPNE115713:CPN0385-MONOMER
- BioCyc:   CPNE138677:CPJ0385-MONOMER
- BioCyc:   CPNE182082:CPB0397-MONOMER
- BRENDA:   3.4.11.1
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00181
- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283
- PANTHER:   PTHR11963:SF3
- PRINTS:   PR00481

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N

EC number: =3.4.11.1; =3.4.11.10

Molecular weight: Translated: 54510; Mature: 54510

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00631 CYTOSOL_AP

Important sites: ACT_SITE 275-275 ACT_SITE 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLFHAQASGRNRVKADAIVLPFWHFKDAKNAASFEAEFEPSYLPALENFQGKTGEIELL
CEEEEECCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEE
YSSPKAKEKRIVLLGLGKNEELTSDVVFQTYATLTRVLRKAKCSTVNIILPTISELRLSA
ECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCH
EEFLVGLSSGILSLNYDYPRYNKVDRNLETPLSKVTVIGIVPKMADAIFRKEAAIFEGVY
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHH
LTRDLVNRNADEITPKKLAEVALNLGKEFPSIDTKVLGKDAIAKEKMGLLLAVSKGSCVD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCEEEEECCCCCCC
PHFIVVRYQGRPKSKDHTVLIGKGVTFDSGGLDLKPGKSMLTMKEDMAGGATVLGILSAL
CEEEEEEECCCCCCCCCEEEEECCEEECCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHH
AVLELPINVTGIIPATENAIDGASYKMGDVYVGMSGLSVEICSTDAEGRLILADAITYAL
HHHHCCCCEEEEECCCCCCCCCCCEEECEEEEECCCCEEEEECCCCCCCEEEHHHHHHHH
KYCKPTRIIDFATLTGAMVVSLGEEVAGFFSNNDVLAEDLLEASAETSEPLWRLPLVKKY
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCHHHHH
DKTLHSDIADMKNLGSNRAGAITAALFLQRFLEESSVAWAHLDIAGTAYHEKEEDRYPKY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCH
ASGFGVRSILYYLENSLSK
HCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLFHAQASGRNRVKADAIVLPFWHFKDAKNAASFEAEFEPSYLPALENFQGKTGEIELL
CEEEEECCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEE
YSSPKAKEKRIVLLGLGKNEELTSDVVFQTYATLTRVLRKAKCSTVNIILPTISELRLSA
ECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCH
EEFLVGLSSGILSLNYDYPRYNKVDRNLETPLSKVTVIGIVPKMADAIFRKEAAIFEGVY
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHH
LTRDLVNRNADEITPKKLAEVALNLGKEFPSIDTKVLGKDAIAKEKMGLLLAVSKGSCVD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCEEEEECCCCCCC
PHFIVVRYQGRPKSKDHTVLIGKGVTFDSGGLDLKPGKSMLTMKEDMAGGATVLGILSAL
CEEEEEEECCCCCCCCCEEEEECCEEECCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHH
AVLELPINVTGIIPATENAIDGASYKMGDVYVGMSGLSVEICSTDAEGRLILADAITYAL
HHHHCCCCEEEEECCCCCCCCCCCEEECEEEEECCCCEEEEECCCCCCCEEEHHHHHHHH
KYCKPTRIIDFATLTGAMVVSLGEEVAGFFSNNDVLAEDLLEASAETSEPLWRLPLVKKY
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCHHHHH
DKTLHSDIADMKNLGSNRAGAITAALFLQRFLEESSVAWAHLDIAGTAYHEKEEDRYPKY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCH
ASGFGVRSILYYLENSLSK
HCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362