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Identifier: 15835874

GI number: 15835874

Start: 383036

End: 383659

Strand: Direct

Name: Not Available

Synonym: CPj0339

Alternate gene names: 15835874

Gene position: 383036-383659 (Clockwise)

Preceding gene: 15835873

Following gene: 15835875

Centisome position: 31.23

GC content: 41.99

Gene sequence:

>624_bases
CTGCCGATGTTTATGAAAATCTGCTCTCTGAAGCTAAAAAATTTTCGTAACCACAGTGATTTAGAAATCTCACTGGCTCC
TAAACTCAATTATGCCCAAGGAAAAACAAACCTCCTAGAAGCGCTTTATGTTTTGTCCTTGGGAAGGTCTTTTCGCACGC
AACATCTCACAGATACCATCACCTTCGGATCTTCCCATTTCTTCTTAGAAACACAGTTTGAGAAAGACCACCTTCCCCAA
GCTCTCTCCATCTATACAGACAAGCAAGGAAAAAAAATCTGCTATAACCAACTTCCTATAAAAACCTTATCGCAGCTGAT
AGGGAAAGTACCTATTGTGCTTTTCTCTTCAAAAGACCGCCTTCTAATTTCAGGAGCTCCTGCGGATCGTCGCCTTTTCC
TAAATCTGCTTTTATCTCAATGCGATAACCACTATACCCTCTGCTTATCGTACTATCATCGCGCTCTTCAGCAGAGAAAT
GCTCTCTTAAAAAGCAAGCAAACCTCAACCGTGGCCTCTGGGATGAACAGTTGGTCAAACACGGCACCTACCTATCCATC
CAACGGTTTCTCTGTAGTCAGAAACTTTCAGATTTATCCAAAGAACTTTGGTCTAACAACCTAA

Upstream 100 bases:

>100_bases
GAATCATTACCGATTCTGCCTCAGGATTATTTGTCATCATGCCTATGAGGCTACATGATGATTAATAAACTCCCTAAGGC
GCACCCTTTAGGTCACTACC

Downstream 100 bases:

>100_bases
AAGAACAATTGGCCTTAAAATTTAAAAGTTCCTTAATTAAAAATTCTGATATTTCTGAAACTGCTGTTGCCGAAGAATTT
CATAAACAGCTCTCTATATC

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPMFMKICSLKLKNFRNHSDLEISLAPKLNYAQGKTNLLEALYVLSLGRSFRTQHLTDTITFGSSHFFLETQFEKDHLPQ
ALSIYTDKQGKKICYNQLPIKTLSQLIGKVPIVLFSSKDRLLISGAPADRRLFLNLLLSQCDNHYTLCLSYYHRALQQRN
ALLKSKQTSTVASGMNSWSNTAPTYPSNGFSVVRNFQIYPKNFGLTT

Sequences:

>Translated_207_residues
MPMFMKICSLKLKNFRNHSDLEISLAPKLNYAQGKTNLLEALYVLSLGRSFRTQHLTDTITFGSSHFFLETQFEKDHLPQ
ALSIYTDKQGKKICYNQLPIKTLSQLIGKVPIVLFSSKDRLLISGAPADRRLFLNLLLSQCDNHYTLCLSYYHRALQQRN
ALLKSKQTSTVASGMNSWSNTAPTYPSNGFSVVRNFQIYPKNFGLTT
>Mature_206_residues
PMFMKICSLKLKNFRNHSDLEISLAPKLNYAQGKTNLLEALYVLSLGRSFRTQHLTDTITFGSSHFFLETQFEKDHLPQA
LSIYTDKQGKKICYNQLPIKTLSQLIGKVPIVLFSSKDRLLISGAPADRRLFLNLLLSQCDNHYTLCLSYYHRALQQRNA
LLKSKQTSTVASGMNSWSNTAPTYPSNGFSVVRNFQIYPKNFGLTT

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=166, Percent_Identity=28.3132530120482, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 23549; Mature: 23417

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMFMKICSLKLKNFRNHSDLEISLAPKLNYAQGKTNLLEALYVLSLGRSFRTQHLTDTI
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHEE
TFGSSHFFLETQFEKDHLPQALSIYTDKQGKKICYNQLPIKTLSQLIGKVPIVLFSSKDR
EECCCEEEEEECCCHHCCCHHHHHEECCCCCEEHHCCCCHHHHHHHHCCCCEEEECCCCC
LLISGAPADRRLFLNLLLSQCDNHYTLCLSYYHRALQQRNALLKSKQTSTVASGMNSWSN
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAPTYPSNGFSVVRNFQIYPKNFGLTT
CCCCCCCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
PMFMKICSLKLKNFRNHSDLEISLAPKLNYAQGKTNLLEALYVLSLGRSFRTQHLTDTI
CHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHEE
TFGSSHFFLETQFEKDHLPQALSIYTDKQGKKICYNQLPIKTLSQLIGKVPIVLFSSKDR
EECCCEEEEEECCCHHCCCHHHHHEECCCCCEEHHCCCCHHHHHHHHCCCCEEEECCCCC
LLISGAPADRRLFLNLLLSQCDNHYTLCLSYYHRALQQRNALLKSKQTSTVASGMNSWSN
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAPTYPSNGFSVVRNFQIYPKNFGLTT
CCCCCCCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]