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The map label for this gene is dnaN

Identifier: 15835873

GI number: 15835873

Start: 381900

End: 383000

Strand: Direct

Name: dnaN

Synonym: CPj0338

Alternate gene names: 15835873

Gene position: 381900-383000 (Clockwise)

Preceding gene: 15835871

Following gene: 15835874

Centisome position: 31.14

GC content: 40.33

Gene sequence:

>1101_bases
ATGAAATTCGTTGTATCCCGAAATGAGCTAGGAAACCTTATCAAAAAAATTCAAAGTGTCGTCCCTCAAAACACACCTAT
TCCAGTACTCACCCATGTTTTGATTGAAACTTATAATGATGAATTAGTTTTCACTGCTACGGATCTGACAGTGAGCACAC
GTTGCGTCACCAAAGCTAAAGTCTATGAGAAAGGCGCTATTTCCATTCCCTCCAAGAGATTTTTTCAATTAGTAAAAGAA
TTAACAGAGGCAAATTTAGAAATTTCCTCTTCAGCAGGGGAAATGGCACAAATCACCTCGGGATCTTCATGCTTTCGCCT
ACTCAGCATGGAAAAAGAAGACTTCCCCATGCTCCCTGATATACAAAATGCTTTGCGTTTTTCCTTGCCTGCAGAGCAGC
TAAAAACCATGCTACAGAGAACTTCATTCGCTGTATCTAGAGAAGAAAGCCGCTATGTTCTTACTGGAGTCCTGCTTGCT
ATCGCCAATGGCGTGGCTACCATCGTAGGGACTGACGGAAAGCGTTTAGCAAAAATAGATGCTGAAGTTACTTTAGATAA
AAGTTTTTCTGGGGAATATATTATTCCTATCAAAGCAGTAGAAGAAATTATAAAGATGTGCTCCGATGAAGGTGAAGCTA
CGATCTTCTTGGATCAAGATAAGATTGCGGTTGAATGTGACAATACTCTCCTGATCACAAAACTTCTTTCTGGAGAATTT
CCAGATTTCTCCCCCGTCATATCTACAGAAAGCAACGTAAAACTCGATCTGCATCGCGAAGAACTAATTACTCTGCTCAA
ACAAGTGGCTTTATTTACAAATGAGTCCTCTCACTCCGTGAAGTTTTCTTTCTTACCCGGAGAGCTCACTCTAACAGCCA
ACTGTACTAAGGTGGGTGAAGGAAAGGTAAGCATGGCTGTAAATTATTCTGGCGAACTCCTAGAAATTGCCTTTAATCCC
TTTTTCTTTTTAGATATCCTGAAGCATAGTAAGGATGAATTAGTCAGCTTAGGGATCTCGGATTCCTATAATCCTGGAAT
CATTACCGATTCTGCCTCAGGATTATTTGTCATCATGCCTATGAGGCTACATGATGATTAA

Upstream 100 bases:

>100_bases
CAGAAAATCACTTTGTAGTAATATCCCGATCGTACCTCTACCGTCTCTCCTAGAGAGGCGTAGCCCTTCATATAAAGTTA
GACTCAGGTTATAGGAAAAT

Downstream 100 bases:

>100_bases
TAAACTCCCTAAGGCGCACCCTTTAGGTCACTACCCTGCCGATGTTTATGAAAATCTGCTCTCTGAAGCTAAAAAATTTT
CGTAACCACAGTGATTTAGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETYNDELVFTATDLTVSTRCVTKAKVYEKGAISIPSKRFFQLVKE
LTEANLEISSSAGEMAQITSGSSCFRLLSMEKEDFPMLPDIQNALRFSLPAEQLKTMLQRTSFAVSREESRYVLTGVLLA
IANGVATIVGTDGKRLAKIDAEVTLDKSFSGEYIIPIKAVEEIIKMCSDEGEATIFLDQDKIAVECDNTLLITKLLSGEF
PDFSPVISTESNVKLDLHREELITLLKQVALFTNESSHSVKFSFLPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVSLGISDSYNPGIITDSASGLFVIMPMRLHDD

Sequences:

>Translated_366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETYNDELVFTATDLTVSTRCVTKAKVYEKGAISIPSKRFFQLVKE
LTEANLEISSSAGEMAQITSGSSCFRLLSMEKEDFPMLPDIQNALRFSLPAEQLKTMLQRTSFAVSREESRYVLTGVLLA
IANGVATIVGTDGKRLAKIDAEVTLDKSFSGEYIIPIKAVEEIIKMCSDEGEATIFLDQDKIAVECDNTLLITKLLSGEF
PDFSPVISTESNVKLDLHREELITLLKQVALFTNESSHSVKFSFLPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVSLGISDSYNPGIITDSASGLFVIMPMRLHDD
>Mature_366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETYNDELVFTATDLTVSTRCVTKAKVYEKGAISIPSKRFFQLVKE
LTEANLEISSSAGEMAQITSGSSCFRLLSMEKEDFPMLPDIQNALRFSLPAEQLKTMLQRTSFAVSREESRYVLTGVLLA
IANGVATIVGTDGKRLAKIDAEVTLDKSFSGEYIIPIKAVEEIIKMCSDEGEATIFLDQDKIAVECDNTLLITKLLSGEF
PDFSPVISTESNVKLDLHREELITLLKQVALFTNESSHSVKFSFLPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVSLGISDSYNPGIITDSASGLFVIMPMRLHDD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=30.5630026809651, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_CHLPN (Q9Z8K0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86533
- PIR:   F81578
- PIR:   H72090
- RefSeq:   NP_224543.1
- RefSeq:   NP_300397.1
- RefSeq:   NP_444967.1
- RefSeq:   NP_876623.1
- ProteinModelPortal:   Q9Z8K0
- SMR:   Q9Z8K0
- PHCI-2DPAGE:   Q9Z8K0
- GeneID:   1467030
- GeneID:   894672
- GeneID:   919112
- GeneID:   963384
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0419
- KEGG:   cpn:CPn0338
- KEGG:   cpt:CpB0347
- TIGR:   CP_0419
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- PhylomeDB:   Q9Z8K0
- ProtClustDB:   PRK05643
- BioCyc:   CPNE115711:CP_0419-MONOMER
- BioCyc:   CPNE115713:CPN0338-MONOMER
- BioCyc:   CPNE138677:CPJ0338-MONOMER
- BioCyc:   CPNE182082:CPB0347-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 40365; Mature: 40365

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETYNDELVFTATDLTVSTRCVTKAK
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEHHHHHHH
VYEKGAISIPSKRFFQLVKELTEANLEISSSAGEMAQITSGSSCFRLLSMEKEDFPMLPD
HHCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCC
IQNALRFSLPAEQLKTMLQRTSFAVSREESRYVLTGVLLAIANGVATIVGTDGKRLAKID
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEC
AEVTLDKSFSGEYIIPIKAVEEIIKMCSDEGEATIFLDQDKIAVECDNTLLITKLLSGEF
CEEEEECCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCEEEEEEHHCCCC
PDFSPVISTESNVKLDLHREELITLLKQVALFTNESSHSVKFSFLPGELTLTANCTKVGE
CCCCCEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCEECCC
GKVSMAVNYSGELLEIAFNPFFFLDILKHSKDELVSLGISDSYNPGIITDSASGLFVIMP
CEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCEEEEEE
MRLHDD
EEECCC
>Mature Secondary Structure
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETYNDELVFTATDLTVSTRCVTKAK
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEHHHHHHH
VYEKGAISIPSKRFFQLVKELTEANLEISSSAGEMAQITSGSSCFRLLSMEKEDFPMLPD
HHCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCC
IQNALRFSLPAEQLKTMLQRTSFAVSREESRYVLTGVLLAIANGVATIVGTDGKRLAKID
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEC
AEVTLDKSFSGEYIIPIKAVEEIIKMCSDEGEATIFLDQDKIAVECDNTLLITKLLSGEF
CEEEEECCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCEEEEEEHHCCCC
PDFSPVISTESNVKLDLHREELITLLKQVALFTNESSHSVKFSFLPGELTLTANCTKVGE
CCCCCEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCEECCC
GKVSMAVNYSGELLEIAFNPFFFLDILKHSKDELVSLGISDSYNPGIITDSASGLFVIMP
CEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCEEEEEE
MRLHDD
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362