Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 15835771

GI number: 15835771

Start: 271778

End: 273391

Strand: Direct

Name: pyrG

Synonym: CPj0236

Alternate gene names: 15835771

Gene position: 271778-273391 (Clockwise)

Preceding gene: 15835770

Following gene: 15835772

Centisome position: 22.16

GC content: 41.7

Gene sequence:

>1614_bases
ATGCCTTTCAAATGCATATTTTTAACAGGAGGAGTTGTCTCCTCTTTAGGAAAAGGGTTAACAGCAGCATCCCTAGCCCT
AATTTTAGAACGTCAACGGCTTAACGTTGCTATGTTAAAATTGGATCCATATCTAAATGTAGATCCAGGAACTATGAATC
CCTTTGAGCATGGAGAAATCTATGTTACAGATGATGGGGTTGAGACAGATCTTGATCTCGGTCACTATCATAGATTCTCT
TCTGCTGCACTTTCTAGACATTCAAGTGCCACTTCAGGTCAAATTTATGCTCGTGTCATTAAAAGAGAGCGTGAGGGTGA
TTATCTAGGAAGCACGGTACAAGTCATCCCACACATTACCAATGAAATCATTCAAGTCATTTTAGACGCAGCTAAAGAGC
ACTCTCCAGATGTTCTTATTGTCGAGATTGGAGGGACCATAGGAGATATTGAATCTCTTCCCTTCCTAGAAGCAATTCGA
CAATTTCGGTATGACCATTCCGAAGATTGTCTAAATATTCATATGACTTATGTCCCCTATTTACAGGCTGCTGACGAAGT
TAAAAGTAAGCCAACGCAACACTCCGTACAAACTCTACGTGGTATTGGCATCATTCCCGACGCGATTCTATGTCGTTCTG
AAAAACCTTTAACTCAAGAAGTTAAATCTAAAATCAGTCTCTTTTGCAATGTTCCCAACCGGGCAGTGTTTAACGTTATA
GATGTAAAACATACCATTTATGAAATGCCTTTGATGCTTGCTCAAGAGAAAATTGCCAATTTCATAGGGGAAAAGTTAAA
GTTAGCTACGGTTCCAGAAAATCTTGATGACTGGAGGGTACTGGTAAATCAGCTATCTCAAGATCTTCCGAAGGTAAAAA
TTGGAGTCGTTGGGAAGTATGTTCAACACCGAGATGCCTATAAGTCCATATTCGAAGCACTCACTCATGCAGCTTTAAGA
TTAGGTCATGCTGCTGAAATTATCCCTATTGATGCTGAAGATGAAAATCTTACTATGGAACTCTCTCAATGCGACGCATG
TTTAGTTCCTGGAGGCTTCGGCGTTCGTGGTTGGGAAGGAAAAATCGCTGCAGCTAAATTCTGTCGAGAACAAGGCATTC
CTTATTTTGGTATTTGCCTAGGAATGCAAGTGCTTGTTGTAGAGTATGCTCGCAATGTCTTAAATCTGGATCAGGCAAAT
TCCCTAGAAATGGACCCCAACACCCCTCATCCTATTGTATATGTCATGGAGGGGCAAGATCCCTTAGTAGCTACGGGAGG
CACCATGCGCTTAGGAGCGTATCCTTGTCTATTAAAGCCAGGGAGCAAAGCCCATAAAGCATATAACGAATCTTCTCTGA
TTCAGGAGCGCCACCGCCATCGCTATGAAGTAAATCCGGATTACATACAGAGTTTAGAAGACCACGGCTTACGGATCGTT
GGGACTTGTCCTCCACAAGGGCTTTGTGAAATTATTGAAGTTTCGGATCATCCTTGGATGATTGGTGTGCAATTCCATCC
AGAATTTGTATCTAAACTCATCTCTCCCCATCCTCTATTTATCGCATTTATAGAAGCAGCTCTAGTCTATTCTAAGGATG
CAAGCCATGTCTAA

Upstream 100 bases:

>100_bases
TCCTAGAACATGGAGGCAAGATCCATGTGTGTATCGTAGATGCAAAAAGTCCCTCTGTTGATTATCCAGAAGACATAGCT
AAAGTAGAACAATATATCAC

Downstream 100 bases:

>100_bases
GCCATCTAGTTGCAAAGCATACCTTGGCATAGACTACGGGAAAAAACGGATCGGCCTTGCCTATGCAGCCGAACCCCTCC
TATTGACACTACCGATTGGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MPFKCIFLTGGVVSSLGKGLTAASLALILERQRLNVAMLKLDPYLNVDPGTMNPFEHGEIYVTDDGVETDLDLGHYHRFS
SAALSRHSSATSGQIYARVIKREREGDYLGSTVQVIPHITNEIIQVILDAAKEHSPDVLIVEIGGTIGDIESLPFLEAIR
QFRYDHSEDCLNIHMTYVPYLQAADEVKSKPTQHSVQTLRGIGIIPDAILCRSEKPLTQEVKSKISLFCNVPNRAVFNVI
DVKHTIYEMPLMLAQEKIANFIGEKLKLATVPENLDDWRVLVNQLSQDLPKVKIGVVGKYVQHRDAYKSIFEALTHAALR
LGHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEGKIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQAN
SLEMDPNTPHPIVYVMEGQDPLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIV
GTCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIEAALVYSKDASHV

Sequences:

>Translated_537_residues
MPFKCIFLTGGVVSSLGKGLTAASLALILERQRLNVAMLKLDPYLNVDPGTMNPFEHGEIYVTDDGVETDLDLGHYHRFS
SAALSRHSSATSGQIYARVIKREREGDYLGSTVQVIPHITNEIIQVILDAAKEHSPDVLIVEIGGTIGDIESLPFLEAIR
QFRYDHSEDCLNIHMTYVPYLQAADEVKSKPTQHSVQTLRGIGIIPDAILCRSEKPLTQEVKSKISLFCNVPNRAVFNVI
DVKHTIYEMPLMLAQEKIANFIGEKLKLATVPENLDDWRVLVNQLSQDLPKVKIGVVGKYVQHRDAYKSIFEALTHAALR
LGHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEGKIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQAN
SLEMDPNTPHPIVYVMEGQDPLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIV
GTCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIEAALVYSKDASHV
>Mature_536_residues
PFKCIFLTGGVVSSLGKGLTAASLALILERQRLNVAMLKLDPYLNVDPGTMNPFEHGEIYVTDDGVETDLDLGHYHRFSS
AALSRHSSATSGQIYARVIKREREGDYLGSTVQVIPHITNEIIQVILDAAKEHSPDVLIVEIGGTIGDIESLPFLEAIRQ
FRYDHSEDCLNIHMTYVPYLQAADEVKSKPTQHSVQTLRGIGIIPDAILCRSEKPLTQEVKSKISLFCNVPNRAVFNVID
VKHTIYEMPLMLAQEKIANFIGEKLKLATVPENLDDWRVLVNQLSQDLPKVKIGVVGKYVQHRDAYKSIFEALTHAALRL
GHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEGKIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQANS
LEMDPNTPHPIVYVMEGQDPLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIVG
TCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIEAALVYSKDASHV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=43.5336976320583, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=43.5336976320583, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=43.5336976320583, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI148491070, Length=550, Percent_Identity=43.6363636363636, Blast_Score=460, Evalue=1e-129,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=49.9082568807339, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=36.3787375415282, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322563, Length=561, Percent_Identity=41.5329768270945, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=39.3238434163701, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24664469, Length=554, Percent_Identity=44.7653429602888, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21357815, Length=498, Percent_Identity=44.3775100401606, Blast_Score=394, Evalue=1e-109,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_CHLPN (Q9Z8U8)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72103
- PIR:   D86520
- PIR:   E81568
- RefSeq:   NP_224445.1
- RefSeq:   NP_300295.1
- RefSeq:   NP_445070.1
- RefSeq:   NP_876518.1
- ProteinModelPortal:   Q9Z8U8
- SMR:   Q9Z8U8
- GeneID:   1466925
- GeneID:   895146
- GeneID:   919011
- GeneID:   962734
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0526
- KEGG:   cpn:CPn0236
- KEGG:   cpt:CpB0242
- TIGR:   CP_0526
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q9Z8U8
- ProtClustDB:   PRK05380
- BioCyc:   CPNE115711:CP_0526-MONOMER
- BioCyc:   CPNE115713:CPN0236-MONOMER
- BioCyc:   CPNE138677:CPJ0236-MONOMER
- BioCyc:   CPNE182082:CPB0242-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59645; Mature: 59514

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 379-379 ACT_SITE 506-506 ACT_SITE 508-508

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKCIFLTGGVVSSLGKGLTAASLALILERQRLNVAMLKLDPYLNVDPGTMNPFEHGEI
CCEEEEEECCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEE
YVTDDGVETDLDLGHYHRFSSAALSRHSSATSGQIYARVIKREREGDYLGSTVQVIPHIT
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NEIIQVILDAAKEHSPDVLIVEIGGTIGDIESLPFLEAIRQFRYDHSEDCLNIHMTYVPY
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHHH
LQAADEVKSKPTQHSVQTLRGIGIIPDAILCRSEKPLTQEVKSKISLFCNVPNRAVFNVI
HHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHEEEECCCCCHHEEHH
DVKHTIYEMPLMLAQEKIANFIGEKLKLATVPENLDDWRVLVNQLSQDLPKVKIGVVGKY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHH
VQHRDAYKSIFEALTHAALRLGHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCEEECCCCCCCCCCCC
KIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQANSLEMDPNTPHPIVYVMEGQD
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
PLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIV
CEEECCCEEEECCCCEEECCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEE
GTCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIEAALVYSKDASHV
ECCCCHHHHHHHEECCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PFKCIFLTGGVVSSLGKGLTAASLALILERQRLNVAMLKLDPYLNVDPGTMNPFEHGEI
CEEEEEECCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEE
YVTDDGVETDLDLGHYHRFSSAALSRHSSATSGQIYARVIKREREGDYLGSTVQVIPHIT
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NEIIQVILDAAKEHSPDVLIVEIGGTIGDIESLPFLEAIRQFRYDHSEDCLNIHMTYVPY
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHHH
LQAADEVKSKPTQHSVQTLRGIGIIPDAILCRSEKPLTQEVKSKISLFCNVPNRAVFNVI
HHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHEEEECCCCCHHEEHH
DVKHTIYEMPLMLAQEKIANFIGEKLKLATVPENLDDWRVLVNQLSQDLPKVKIGVVGKY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHH
VQHRDAYKSIFEALTHAALRLGHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCEEECCCCCCCCCCCC
KIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQANSLEMDPNTPHPIVYVMEGQD
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
PLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIV
CEEECCCEEEECCCCEEECCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEE
GTCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIEAALVYSKDASHV
ECCCCHHHHHHHEECCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362