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The map label for this gene is kdsB

Identifier: 15835770

GI number: 15835770

Start: 271038

End: 271802

Strand: Direct

Name: kdsB

Synonym: CPj0235

Alternate gene names: 15835770

Gene position: 271038-271802 (Clockwise)

Preceding gene: 15835764

Following gene: 15835771

Centisome position: 22.1

GC content: 40.26

Gene sequence:

>765_bases
ATGAAGCCGGAAGAGTCTGAGTGTCTGTGTATTGGAGTTTTGCCCGCACGCTGGAATAGCAGTCGCTATCCAGGAAAGCC
TTTGGCTAAAATTCATGGAAAAAGCTTAATACAAAGAACTTATGAGAATGCTTCCCAAAGTTCTCTATTAGATAAAATTG
TTGTTGCTACTGACGATCAGCATATTATCGACCACGTGACTGATTTTGGTGGTTATGCAGTGATGACTTCTCCTACATGT
TCCAATGGTACAGAACGCACAGGTGAAGTAGCTAGAAAGTACTTCCCTAAAGCTGAGATTATTGTAAATATTCAAGGTGA
TGAGCCTTGTCTAAATTCTGAGGTTGTCGACGCTTTGGTTCAGAAGTTGAGAAGTTCTCCTGAAGCAGAACTGGTGACTC
CTGTGGCACTCACGACAGATCGTGAAGAGATCTTAACAGAAAAAAAAGTAAAATGTGTTTTTGACTCTGAGGGAAGGGCT
CTGTATTTTAGTCGCAGTCCTATTCCTTTTATTCTTAAAAAAGCAACCCCAGTATATCTCCATATTGGAGTATATGCTTT
TAAAAGAGAGGCTCTTTTCCGCTACCTACAGCATAGCTCAACTCCTCTAAGCGATGCCGAAGATCTTGAGCAATTACGTT
TCCTAGAACATGGAGGCAAGATCCATGTGTGTATCGTAGATGCAAAAAGTCCCTCTGTTGATTATCCAGAAGACATAGCT
AAAGTAGAACAATATATCACATGCCTTTCAAATGCATATTTTTAA

Upstream 100 bases:

>100_bases
CTTTTTTTTCAGAAAAATAAAAAAAATATTGCGTTTTATAAAATGCATCACAATAATCCTGGTAGTCTTAAACACATAAG
TTTTTGTTAGGTATCTCCTT

Downstream 100 bases:

>100_bases
CAGGAGGAGTTGTCTCCTCTTTAGGAAAAGGGTTAACAGCAGCATCCCTAGCCCTAATTTTAGAACGTCAACGGCTTAAC
GTTGCTATGTTAAAATTGGA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKPEESECLCIGVLPARWNSSRYPGKPLAKIHGKSLIQRTYENASQSSLLDKIVVATDDQHIIDHVTDFGGYAVMTSPTC
SNGTERTGEVARKYFPKAEIIVNIQGDEPCLNSEVVDALVQKLRSSPEAELVTPVALTTDREEILTEKKVKCVFDSEGRA
LYFSRSPIPFILKKATPVYLHIGVYAFKREALFRYLQHSSTPLSDAEDLEQLRFLEHGGKIHVCIVDAKSPSVDYPEDIA
KVEQYITCLSNAYF

Sequences:

>Translated_254_residues
MKPEESECLCIGVLPARWNSSRYPGKPLAKIHGKSLIQRTYENASQSSLLDKIVVATDDQHIIDHVTDFGGYAVMTSPTC
SNGTERTGEVARKYFPKAEIIVNIQGDEPCLNSEVVDALVQKLRSSPEAELVTPVALTTDREEILTEKKVKCVFDSEGRA
LYFSRSPIPFILKKATPVYLHIGVYAFKREALFRYLQHSSTPLSDAEDLEQLRFLEHGGKIHVCIVDAKSPSVDYPEDIA
KVEQYITCLSNAYF
>Mature_254_residues
MKPEESECLCIGVLPARWNSSRYPGKPLAKIHGKSLIQRTYENASQSSLLDKIVVATDDQHIIDHVTDFGGYAVMTSPTC
SNGTERTGEVARKYFPKAEIIVNIQGDEPCLNSEVVDALVQKLRSSPEAELVTPVALTTDREEILTEKKVKCVFDSEGRA
LYFSRSPIPFILKKATPVYLHIGVYAFKREALFRYLQHSSTPLSDAEDLEQLRFLEHGGKIHVCIVDAKSPSVDYPEDIA
KVEQYITCLSNAYF

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=242, Percent_Identity=37.603305785124, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_CHLPN (Q9Z8U9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86520
- PIR:   H72102
- RefSeq:   NP_224444.1
- RefSeq:   NP_300294.1
- RefSeq:   NP_445071.1
- RefSeq:   NP_876517.1
- ProteinModelPortal:   Q9Z8U9
- SMR:   Q9Z8U9
- PHCI-2DPAGE:   Q9Z8U9
- GeneID:   1466924
- GeneID:   895121
- GeneID:   919018
- GeneID:   963770
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0527
- KEGG:   cpn:CPn0235
- KEGG:   cpt:CpB0241
- TIGR:   CP_0527
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   Q9Z8U9
- ProtClustDB:   PRK05450
- BioCyc:   CPNE115711:CP_0527-MONOMER
- BioCyc:   CPNE115713:CPN0235-MONOMER
- BioCyc:   CPNE138677:CPJ0235-MONOMER
- BioCyc:   CPNE182082:CPB0241-MONOMER
- BRENDA:   2.7.7.38
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28415; Mature: 28415

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEESECLCIGVLPARWNSSRYPGKPLAKIHGKSLIQRTYENASQSSLLDKIVVATDDQ
CCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCH
HIIDHVTDFGGYAVMTSPTCSNGTERTGEVARKYFPKAEIIVNIQGDEPCLNSEVVDALV
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
QKLRSSPEAELVTPVALTTDREEILTEKKVKCVFDSEGRALYFSRSPIPFILKKATPVYL
HHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEEECCCCEEEEECCCCCEEEECCCEEEE
HIGVYAFKREALFRYLQHSSTPLSDAEDLEQLRFLEHGGKIHVCIVDAKSPSVDYPEDIA
EEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHH
KVEQYITCLSNAYF
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKPEESECLCIGVLPARWNSSRYPGKPLAKIHGKSLIQRTYENASQSSLLDKIVVATDDQ
CCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCH
HIIDHVTDFGGYAVMTSPTCSNGTERTGEVARKYFPKAEIIVNIQGDEPCLNSEVVDALV
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
QKLRSSPEAELVTPVALTTDREEILTEKKVKCVFDSEGRALYFSRSPIPFILKKATPVYL
HHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEEECCCCEEEEECCCCCEEEECCCEEEE
HIGVYAFKREALFRYLQHSSTPLSDAEDLEQLRFLEHGGKIHVCIVDAKSPSVDYPEDIA
EEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHH
KVEQYITCLSNAYF
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362