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The map label for this gene is tpiA

Identifier: 15835221

GI number: 15835221

Start: 723587

End: 724399

Strand: Direct

Name: tpiA

Synonym: TC0604

Alternate gene names: 15835221

Gene position: 723587-724399 (Clockwise)

Preceding gene: 15835216

Following gene: 15835222

Centisome position: 67.44

GC content: 41.82

Gene sequence:

>813_bases
ATGCTTACAGATAAAAAAAGTTCTCCTACTTGGTCTAGTCTTCTCCCCTCCGAAACATCACAATATTTTGTTTTCGGTAA
CTGGAAAATGAACAAAACTTTTAGCGAAGCTCAGACCTTCTTAAAAGATTTTGTTTCTTGTGAAATTCTCTCTAATCCTA
AAATCATTACAGGGATTATACCCCCGTTCACACTTCTGTCCTCCTGTCAGCAAATCATAAAGAATACCCCTATCCGTTTA
GGAGCCCAAACTTTACACGAGGTGGATTCAGGAGCATTTACTGGGGAAATTTCAGCTCCAATGCTCAAAGACATCGGAGT
CGATTTTGTCCTCATTGGGCATTCTGAAAGACGCCACATCTTCCATGAACAAAATCACTCTCTTGCAGAAAAACTACTCG
CAGCGATTCGTAACGGAATCGTTCCCGTTCTTTGTATCGGAGAAACCCTAGAAGAACAAGAAGCTGGAGCAACTCAAGAT
ATTCTTTTAGAACAACTAACCGTAGGGTTATCTCGACTCCCAGAACATGCCCCCTTCATTCTAGCTTATGAACCGGTCTG
GGCTATCGGCACAGGGAAAGTAGCTAATCCTGACTTAGTTCAAGAAATTCATGCTTTCTGTAGAAATGTCGTCAAAGATC
TTATTTCTAAGGATGCTGCCGAGCGCACTCCTATTCTTTATGGAGGGTCTGTGAAAGCTGATAATACTCGCGCACTTACT
CTCTGTCCGGACGTTAACGGACTTTTAGTTGGAGGAGCTTCTCTATCTGTAGAGAGTTTTCTTGCTATTATACAACAAAT
CGCTGTCTCATAA

Upstream 100 bases:

>100_bases
TAAAAAATAAATCGGATAAAATAAGCCCCTCTTGCAAGAAATCGAATATTACGCTTTGATCTATTCTACGAAATGAGTGC
ACAGGTTATTCGGAAATTCC

Downstream 100 bases:

>100_bases
ATCATAGTACCTATGTCGATAACATCTCCTCCTGTAGAAGTCTCTGTCCTTACAGATTCTATTAAGAATCTTTTAGAAAA
AAATTTTCTTCGGGTAGTGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLTDKKSSPTWSSLLPSETSQYFVFGNWKMNKTFSEAQTFLKDFVSCEILSNPKIITGIIPPFTLLSSCQQIIKNTPIRL
GAQTLHEVDSGAFTGEISAPMLKDIGVDFVLIGHSERRHIFHEQNHSLAEKLLAAIRNGIVPVLCIGETLEEQEAGATQD
ILLEQLTVGLSRLPEHAPFILAYEPVWAIGTGKVANPDLVQEIHAFCRNVVKDLISKDAAERTPILYGGSVKADNTRALT
LCPDVNGLLVGGASLSVESFLAIIQQIAVS

Sequences:

>Translated_270_residues
MLTDKKSSPTWSSLLPSETSQYFVFGNWKMNKTFSEAQTFLKDFVSCEILSNPKIITGIIPPFTLLSSCQQIIKNTPIRL
GAQTLHEVDSGAFTGEISAPMLKDIGVDFVLIGHSERRHIFHEQNHSLAEKLLAAIRNGIVPVLCIGETLEEQEAGATQD
ILLEQLTVGLSRLPEHAPFILAYEPVWAIGTGKVANPDLVQEIHAFCRNVVKDLISKDAAERTPILYGGSVKADNTRALT
LCPDVNGLLVGGASLSVESFLAIIQQIAVS
>Mature_270_residues
MLTDKKSSPTWSSLLPSETSQYFVFGNWKMNKTFSEAQTFLKDFVSCEILSNPKIITGIIPPFTLLSSCQQIIKNTPIRL
GAQTLHEVDSGAFTGEISAPMLKDIGVDFVLIGHSERRHIFHEQNHSLAEKLLAAIRNGIVPVLCIGETLEEQEAGATQD
ILLEQLTVGLSRLPEHAPFILAYEPVWAIGTGKVANPDLVQEIHAFCRNVVKDLISKDAAERTPILYGGSVKADNTRALT
LCPDVNGLLVGGASLSVESFLAIIQQIAVS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=39.9209486166008, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=39.8373983739837, Blast_Score=187, Evalue=6e-48,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=40.2439024390244, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=42.5101214574899, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=244, Percent_Identity=40.5737704918033, Blast_Score=182, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=43.5483870967742, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=43.5483870967742, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=43.3734939759036, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CHLMU (Q9PK66)

Other databases:

- EMBL:   AE002160
- PIR:   A81684
- RefSeq:   NP_296980.1
- ProteinModelPortal:   Q9PK66
- SMR:   Q9PK66
- GeneID:   1245966
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0604
- TIGR:   TC_0604
- HOGENOM:   HBG708281
- OMA:   CIGENLD
- PhylomeDB:   Q9PK66
- ProtClustDB:   PRK00042
- BioCyc:   CMUR243161:TC_0604-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 29408; Mature: 29408

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 114-114 ACT_SITE 184-184 BINDING 27-27 BINDING 29-29

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDKKSSPTWSSLLPSETSQYFVFGNWKMNKTFSEAQTFLKDFVSCEILSNPKIITGII
CCCCCCCCCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHHHEEECCCCCEEEECC
PPFTLLSSCQQIIKNTPIRLGAQTLHEVDSGAFTGEISAPMLKDIGVDFVLIGHSERRHI
CHHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCHHHH
FHEQNHSLAEKLLAAIRNGIVPVLCIGETLEEQEAGATQDILLEQLTVGLSRLPEHAPFI
HHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEE
LAYEPVWAIGTGKVANPDLVQEIHAFCRNVVKDLISKDAAERTPILYGGSVKADNTRALT
EEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCEECCCCCEEEE
LCPDVNGLLVGGASLSVESFLAIIQQIAVS
ECCCCCEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTDKKSSPTWSSLLPSETSQYFVFGNWKMNKTFSEAQTFLKDFVSCEILSNPKIITGII
CCCCCCCCCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHHHEEECCCCCEEEECC
PPFTLLSSCQQIIKNTPIRLGAQTLHEVDSGAFTGEISAPMLKDIGVDFVLIGHSERRHI
CHHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCHHHH
FHEQNHSLAEKLLAAIRNGIVPVLCIGETLEEQEAGATQDILLEQLTVGLSRLPEHAPFI
HHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEE
LAYEPVWAIGTGKVANPDLVQEIHAFCRNVVKDLISKDAAERTPILYGGSVKADNTRALT
EEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCEECCCCCEEEE
LCPDVNGLLVGGASLSVESFLAIIQQIAVS
ECCCCCEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935