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Identifier: 15835157

GI number: 15835157

Start: 649474

End: 650280

Strand: Reverse

Name: Not Available

Synonym: TC0539

Alternate gene names: 15835157

Gene position: 650280-649474 (Counterclockwise)

Preceding gene: 15835158

Following gene: 15835156

Centisome position: 60.61

GC content: 40.02

Gene sequence:

>807_bases
TTGCAGGGAAGGGACATGAGGTATATCAGATCTTCAAACATCAGACGATTGTCTTTGATGATCGAGAGGTTGTGTGTGAA
GCTTTGGCCTCGATTTATTAATTCAAAATTCTTGTTTTTACTGCTTGCTTTGGGTGTCGGTCCGGATAAGTGTTTTTCGG
ATGCTGTAAATACTCCTGTTGTTCCCAAGGTAAGTCGAAATGAGCTTATTGTGATAGATCCTGGCCATGGTGGTAAGGAT
GAAGGAACTGCGGATAAGGAGCTGCGATATAAAGAGAAGTCTTTAGCTTTATCAATAGCTTTAAGTGTGCAGGGGTATCT
ACGCCGAATGGGATATAAGACGATCATGACAAGATCCACAGATGTTTATGTGGATTTGAATAAGCGAGCGGCGATAGCAA
ACCAAAACAAAGCAGATGTTTTTGTTAGTATCCATTGTAATTACTCCTCTAATACCTCTGCTTTAGGTACAGAGGTCTAT
TTTTATAATGATAAAAATGTATTGAGAACTAAAAAATCAGAGAACCTTGGGAAATCGATTCTAGCTTTCATGCAAAAGAA
TGGGGCTTTGCGGGAGCGTAAGGTTAAAGAGGGTAATTTTGCAGTTATCCGAGAAACCTCCATGCCTGCGGTCCTGGTAG
AAACAGGGTTCCTCTCTAATCCAAAAGAGCGAGCAGCTCTTTTAGACTCCCGTTATCGTTCGCATTTAGCTAAAGGTATT
TCTGAAGGAATACATGCCTTTATTCTTAATCGACAGATTAAAAAGACTACAGCGGGGAATTCGGGAGTAAAAAAAGTGTA
TAAATAA

Upstream 100 bases:

>100_bases
TGTTCAGGATTTTCAACAGATCGCTATGTTATTGAAAGTGATAGAAGATTAGCCATCGTTAAAGCAATATCAATGGCTTT
GGATAAGGATATTGTGTTAG

Downstream 100 bases:

>100_bases
TCTGGGGGAGAGAGGGCTTTAACAAAACCTTTAAGTATTCGAAAAGATATTCTTAGTAGCTGAGGTTTCAGTTGAGTGCA
ACTCACGGGTAGATTTTGTG

Product: N-acetylmuramoyl-L-alanine amidase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQGRDMRYIRSSNIRRLSLMIERLCVKLWPRFINSKFLFLLLALGVGPDKCFSDAVNTPVVPKVSRNELIVIDPGHGGKD
EGTADKELRYKEKSLALSIALSVQGYLRRMGYKTIMTRSTDVYVDLNKRAAIANQNKADVFVSIHCNYSSNTSALGTEVY
FYNDKNVLRTKKSENLGKSILAFMQKNGALRERKVKEGNFAVIRETSMPAVLVETGFLSNPKERAALLDSRYRSHLAKGI
SEGIHAFILNRQIKKTTAGNSGVKKVYK

Sequences:

>Translated_268_residues
MQGRDMRYIRSSNIRRLSLMIERLCVKLWPRFINSKFLFLLLALGVGPDKCFSDAVNTPVVPKVSRNELIVIDPGHGGKD
EGTADKELRYKEKSLALSIALSVQGYLRRMGYKTIMTRSTDVYVDLNKRAAIANQNKADVFVSIHCNYSSNTSALGTEVY
FYNDKNVLRTKKSENLGKSILAFMQKNGALRERKVKEGNFAVIRETSMPAVLVETGFLSNPKERAALLDSRYRSHLAKGI
SEGIHAFILNRQIKKTTAGNSGVKKVYK
>Mature_268_residues
MQGRDMRYIRSSNIRRLSLMIERLCVKLWPRFINSKFLFLLLALGVGPDKCFSDAVNTPVVPKVSRNELIVIDPGHGGKD
EGTADKELRYKEKSLALSIALSVQGYLRRMGYKTIMTRSTDVYVDLNKRAAIANQNKADVFVSIHCNYSSNTSALGTEVY
FYNDKNVLRTKKSENLGKSILAFMQKNGALRERKVKEGNFAVIRETSMPAVLVETGFLSNPKERAALLDSRYRSHLAKGI
SEGIHAFILNRQIKKTTAGNSGVKKVYK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=29.3103448275862, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082163, Length=275, Percent_Identity=28, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30128; Mature: 30128

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGRDMRYIRSSNIRRLSLMIERLCVKLWPRFINSKFLFLLLALGVGPDKCFSDAVNTPV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHCCCCC
VPKVSRNELIVIDPGHGGKDEGTADKELRYKEKSLALSIALSVQGYLRRMGYKTIMTRST
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEHHHHHHHHHCCCHHEEECCC
DVYVDLNKRAAIANQNKADVFVSIHCNYSSNTSALGTEVYFYNDKNVLRTKKSENLGKSI
EEEEEECCCEEECCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCHHHHHHHH
LAFMQKNGALRERKVKEGNFAVIRETSMPAVLVETGFLSNPKERAALLDSRYRSHLAKGI
HHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
SEGIHAFILNRQIKKTTAGNSGVKKVYK
HHHHHHHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MQGRDMRYIRSSNIRRLSLMIERLCVKLWPRFINSKFLFLLLALGVGPDKCFSDAVNTPV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHCCCCC
VPKVSRNELIVIDPGHGGKDEGTADKELRYKEKSLALSIALSVQGYLRRMGYKTIMTRST
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEHHHHHHHHHCCCHHEEECCC
DVYVDLNKRAAIANQNKADVFVSIHCNYSSNTSALGTEVYFYNDKNVLRTKKSENLGKSI
EEEEEECCCEEECCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCHHHHHHHH
LAFMQKNGALRERKVKEGNFAVIRETSMPAVLVETGFLSNPKERAALLDSRYRSHLAKGI
HHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
SEGIHAFILNRQIKKTTAGNSGVKKVYK
HHHHHHHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]