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The map label for this gene is murE

Identifier: 15835158

GI number: 15835158

Start: 650180

End: 651640

Strand: Reverse

Name: murE

Synonym: TC0540

Alternate gene names: 15835158

Gene position: 651640-650180 (Counterclockwise)

Preceding gene: 15835160

Following gene: 15835157

Centisome position: 60.73

GC content: 40.45

Gene sequence:

>1461_bases
GTGTTCGGCATGCATTTAGACCAGCTTCTTCGGGATATTCCGGCTAAAATCTATGGGAAAGTTGAGTCTATTCCTGTTAG
AAATTTGACTCGAGACTCTCGTTGTACGGGGGTGGGAGATATTTTTATTGCTCGCCAAGGTCAAATGTGTAATGGAAACG
ACTATTCAGGCCAAGCTGTTGAGAATGGCGCAATTGCCATTCTTTCTTCTTTATATAATCCCTTTCTATCAGTTGTTCAG
ATTGTTACAGAAGATCTTACTGCTTTGGAGGCTTGTTTAGCAGCACGATTCTATAATGATCCTTCGAAACGCCTGGATGT
TATAGGGGTTACAGGAACTAATGGGAAAACAACGGTTTCTGCTTTAGCTAGAGAGCTCATGGAGTATAAAGGGCGATGTA
CAGGGCTTGTTGGGACTATAGAGCATATTTTAGGAGAACACCGAATTATAGATAGTTTTACGACTCCAGATGCTATCTTA
TTGCAAAAGTACTTTGCTGAAATGGTTAAACAGAACTTATCATCAGCTGTTGTGGAAGTATCCTCGATAGGACTTGCTCT
TGGTAGGGTTCGCGAAACGGAATTTTTAGCAGGGGTTCTGACTAATGTCTCGTTAGATCATTTAGATTTTCATGGTTCTT
TTGAGGAGTATGTTGTTGCTAAAAAGCAGTTATTTGTTTCTCTCCCAGAGCATGGGGTTGCGGTTGTTAATTCGGATTGT
GAGTATGCGCAAAGCTTTTTAGAAATTTCTCCTGCCAGAGGAGTTTCTTATGCCGTACATCAAGAGGCGGATTATCGCGC
TGTAAATCTGAAATTTTCTTCTTTGGGGTCCACTTTCGATATCTTATATCAGGGAAATGTTTTCTCTTGTGAGACCTCTT
TGGTAGGGGAACATAATGTGTACAATGTTTTAGCTGCTCTGTCAGTAGTCCATCAAATTCTGGGAGGAGATTTTGCAGAG
CTAGTACATTATGTTCGTTATTTATCTGCTCCAAAAGGGCGTTTGGAGCCAGTTCTATCTGGTCCTTGTCCTATTTATAT
CGATTATGCACATACTCCAGATGCATTGGATAATGTATGTAAAATCTTATCGCAACTGCTTCCTGCAGATGGACGGTTAA
TTATTGTATTCGGATGTGGTGGGGATCGGGATCATAGCAAGAGGCCTATTATGGCAAAGGTTGCTGAGACTTATGGTTTT
TCTGTTGTCACTTCAGATAATCCCAGAACTGAAGATCCAGATCAGATCATTGCTGATATATGTTCAGGATTTTCAACAGA
TCGCTATGTTATTGAAAGTGATAGAAGATTAGCCATCGTTAAAGCAATATCAATGGCTTTGGATAAGGATATTGTGTTAG
TTGCAGGGAAGGGACATGAGGTATATCAGATCTTCAAACATCAGACGATTGTCTTTGATGATCGAGAGGTTGTGTGTGAA
GCTTTGGCCTCGATTTATTAA

Upstream 100 bases:

>100_bases
CTAATGTGCGAGTGTTAGTTGTGGACAAAGCTTGACAACGAATGTTTGTATAGTAAATTATTCGAGAACGCTTTTTGAAG
CCTTTGCATACGTTTTTTAG

Downstream 100 bases:

>100_bases
TTCAAAATTCTTGTTTTTACTGCTTGCTTTGGGTGTCGGTCCGGATAAGTGTTTTTCGGATGCTGTAAATACTCCTGTTG
TTCCCAAGGTAAGTCGAAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MFGMHLDQLLRDIPAKIYGKVESIPVRNLTRDSRCTGVGDIFIARQGQMCNGNDYSGQAVENGAIAILSSLYNPFLSVVQ
IVTEDLTALEACLAARFYNDPSKRLDVIGVTGTNGKTTVSALARELMEYKGRCTGLVGTIEHILGEHRIIDSFTTPDAIL
LQKYFAEMVKQNLSSAVVEVSSIGLALGRVRETEFLAGVLTNVSLDHLDFHGSFEEYVVAKKQLFVSLPEHGVAVVNSDC
EYAQSFLEISPARGVSYAVHQEADYRAVNLKFSSLGSTFDILYQGNVFSCETSLVGEHNVYNVLAALSVVHQILGGDFAE
LVHYVRYLSAPKGRLEPVLSGPCPIYIDYAHTPDALDNVCKILSQLLPADGRLIIVFGCGGDRDHSKRPIMAKVAETYGF
SVVTSDNPRTEDPDQIIADICSGFSTDRYVIESDRRLAIVKAISMALDKDIVLVAGKGHEVYQIFKHQTIVFDDREVVCE
ALASIY

Sequences:

>Translated_486_residues
MFGMHLDQLLRDIPAKIYGKVESIPVRNLTRDSRCTGVGDIFIARQGQMCNGNDYSGQAVENGAIAILSSLYNPFLSVVQ
IVTEDLTALEACLAARFYNDPSKRLDVIGVTGTNGKTTVSALARELMEYKGRCTGLVGTIEHILGEHRIIDSFTTPDAIL
LQKYFAEMVKQNLSSAVVEVSSIGLALGRVRETEFLAGVLTNVSLDHLDFHGSFEEYVVAKKQLFVSLPEHGVAVVNSDC
EYAQSFLEISPARGVSYAVHQEADYRAVNLKFSSLGSTFDILYQGNVFSCETSLVGEHNVYNVLAALSVVHQILGGDFAE
LVHYVRYLSAPKGRLEPVLSGPCPIYIDYAHTPDALDNVCKILSQLLPADGRLIIVFGCGGDRDHSKRPIMAKVAETYGF
SVVTSDNPRTEDPDQIIADICSGFSTDRYVIESDRRLAIVKAISMALDKDIVLVAGKGHEVYQIFKHQTIVFDDREVVCE
ALASIY
>Mature_486_residues
MFGMHLDQLLRDIPAKIYGKVESIPVRNLTRDSRCTGVGDIFIARQGQMCNGNDYSGQAVENGAIAILSSLYNPFLSVVQ
IVTEDLTALEACLAARFYNDPSKRLDVIGVTGTNGKTTVSALARELMEYKGRCTGLVGTIEHILGEHRIIDSFTTPDAIL
LQKYFAEMVKQNLSSAVVEVSSIGLALGRVRETEFLAGVLTNVSLDHLDFHGSFEEYVVAKKQLFVSLPEHGVAVVNSDC
EYAQSFLEISPARGVSYAVHQEADYRAVNLKFSSLGSTFDILYQGNVFSCETSLVGEHNVYNVLAALSVVHQILGGDFAE
LVHYVRYLSAPKGRLEPVLSGPCPIYIDYAHTPDALDNVCKILSQLLPADGRLIIVFGCGGDRDHSKRPIMAKVAETYGF
SVVTSDNPRTEDPDQIIADICSGFSTDRYVIESDRRLAIVKAISMALDKDIVLVAGKGHEVYQIFKHQTIVFDDREVVCE
ALASIY

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=504, Percent_Identity=36.5079365079365, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1786274, Length=343, Percent_Identity=25.0728862973761, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53277; Mature: 53277

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGMHLDQLLRDIPAKIYGKVESIPVRNLTRDSRCTGVGDIFIARQGQMCNGNDYSGQAV
CCCCCHHHHHHHCCHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
ENGAIAILSSLYNPFLSVVQIVTEDLTALEACLAARFYNDPSKRLDVIGVTGTNGKTTVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
ALARELMEYKGRCTGLVGTIEHILGEHRIIDSFTTPDAILLQKYFAEMVKQNLSSAVVEV
HHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SSIGLALGRVRETEFLAGVLTNVSLDHLDFHGSFEEYVVAKKQLFVSLPEHGVAVVNSDC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCCEEEEECCH
EYAQSFLEISPARGVSYAVHQEADYRAVNLKFSSLGSTFDILYQGNVFSCETSLVGEHNV
HHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHHCCCEEEEEEECCEEEECCCCCCCCHH
YNVLAALSVVHQILGGDFAELVHYVRYLSAPKGRLEPVLSGPCPIYIDYAHTPDALDNVC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
KILSQLLPADGRLIIVFGCGGDRDHSKRPIMAKVAETYGFSVVTSDNPRTEDPDQIIADI
HHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHH
CSGFSTDRYVIESDRRLAIVKAISMALDKDIVLVAGKGHEVYQIFKHQTIVFDDREVVCE
HCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCEEEECCHHHHHH
ALASIY
HHHHCC
>Mature Secondary Structure
MFGMHLDQLLRDIPAKIYGKVESIPVRNLTRDSRCTGVGDIFIARQGQMCNGNDYSGQAV
CCCCCHHHHHHHCCHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
ENGAIAILSSLYNPFLSVVQIVTEDLTALEACLAARFYNDPSKRLDVIGVTGTNGKTTVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
ALARELMEYKGRCTGLVGTIEHILGEHRIIDSFTTPDAILLQKYFAEMVKQNLSSAVVEV
HHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SSIGLALGRVRETEFLAGVLTNVSLDHLDFHGSFEEYVVAKKQLFVSLPEHGVAVVNSDC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCCEEEEECCH
EYAQSFLEISPARGVSYAVHQEADYRAVNLKFSSLGSTFDILYQGNVFSCETSLVGEHNV
HHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHHCCCEEEEEEECCEEEECCCCCCCCHH
YNVLAALSVVHQILGGDFAELVHYVRYLSAPKGRLEPVLSGPCPIYIDYAHTPDALDNVC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
KILSQLLPADGRLIIVFGCGGDRDHSKRPIMAKVAETYGFSVVTSDNPRTEDPDQIIADI
HHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHH
CSGFSTDRYVIESDRRLAIVKAISMALDKDIVLVAGKGHEVYQIFKHQTIVFDDREVVCE
HCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCEEEECCHHHHHH
ALASIY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]