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The map label for this gene is pdhB

Identifier: 15835135

GI number: 15835135

Start: 624747

End: 625733

Strand: Direct

Name: pdhB

Synonym: TC0517

Alternate gene names: 15835135

Gene position: 624747-625733 (Clockwise)

Preceding gene: 15835134

Following gene: 15835136

Centisome position: 58.23

GC content: 39.92

Gene sequence:

>987_bases
ATGCCTAATTTTGTTACTCTCGAAATTCGAGAAGCCATAAGACAAGCTATTGATGAGGAAATGACTAGGGATCCTAATGT
ATGCATTTTAGGAGAAGAAGTCGCTGAATATAATGGGGCTTACAAAGTCACTAAAAATCTTTTAGATAAATGGGGACCGA
CCCGAGTTATTGATACGCCCATTAGCGAAGCTGCATTTTCCGGGATTGGAATTGGCGCGGCACTAACCGGATTACGCCCA
ATTATTGAATTTATGAGCTGGAACTTCTCTTTAGTCGCTGCAGATCAAATCATTTCTCATGCGGCAAAAATGCATTACAT
GACCGGAGGGAAATTTTCTGTTCCCATTGTTTTTAGAGGAGCCAATGGAGCTGCTGCACAAGTCTCTTGTCAACATTCTC
ATTGTGTAGAAGCCCTTTATGCAAATATTCCAGGCTTAATTATCATTGCTCCATCAACCCCAGCAGATGCCAAAGGGCTT
CTTAAAGCTGCTATTCGTGATAATAATCCCGTCCTATTTTTAGAGAATGAATTGGACTATAACTTGAAGGGAGAAGTTCC
TACAGAGGAGTATCTTGTCCCTATTGGGAAAGCTCACATTGTTCAAGAAGGACTAGACTTAACCATCATTTCGCATAGCC
GTATGGTAACTATCGTTGAGCTAGCTGCTAAAATAGCAAAACAGAGATGGGGATTTTCTATTGAAATTTTAGATTTGCGA
ACAATTAAACCTTTGGATATTGCCGCAATTCTAACTTCCGTTAAAAAAACTGGAAATTGTCTTGTTGTGGAAGAGGGGCA
TTATTTTTGTGGAATATCCGCAGAAATCATTGCAACCATTACAGAGCATATTTTTGATCACTTGGACCATCCTCCTTTAC
GAGTTTGTCAAAAAGAAACTCCTATGCCATACAGTAAAACACTGGAGACGGCGACTCTTCCTAATGTTAACCGCATCCTG
GATGCCATTGAAAAAATTATGAGGTAA

Upstream 100 bases:

>100_bases
AAAGATTTACGGCAAACCAGCAAAGAGGCCGTTCTAGAAGCATTCTCCCAAGCTCGTCTTGATCCAGAACCTGCTGTTGC
CACTTTAGAAGAGGGGATCT

Downstream 100 bases:

>100_bases
CGTTGTGGTTTCTTTGTTAAAAATGCCTAAGCTATCCCCTACAATGGAAACAGGGACCCTTGTTAAATGGCATAAACAAG
CTGGTGATGAAGTCCATTTT

Product: pyruvate dehydrogenase, E1 component, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPNFVTLEIREAIRQAIDEEMTRDPNVCILGEEVAEYNGAYKVTKNLLDKWGPTRVIDTPISEAAFSGIGIGAALTGLRP
IIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGANGAAAQVSCQHSHCVEALYANIPGLIIIAPSTPADAKGL
LKAAIRDNNPVLFLENELDYNLKGEVPTEEYLVPIGKAHIVQEGLDLTIISHSRMVTIVELAAKIAKQRWGFSIEILDLR
TIKPLDIAAILTSVKKTGNCLVVEEGHYFCGISAEIIATITEHIFDHLDHPPLRVCQKETPMPYSKTLETATLPNVNRIL
DAIEKIMR

Sequences:

>Translated_328_residues
MPNFVTLEIREAIRQAIDEEMTRDPNVCILGEEVAEYNGAYKVTKNLLDKWGPTRVIDTPISEAAFSGIGIGAALTGLRP
IIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGANGAAAQVSCQHSHCVEALYANIPGLIIIAPSTPADAKGL
LKAAIRDNNPVLFLENELDYNLKGEVPTEEYLVPIGKAHIVQEGLDLTIISHSRMVTIVELAAKIAKQRWGFSIEILDLR
TIKPLDIAAILTSVKKTGNCLVVEEGHYFCGISAEIIATITEHIFDHLDHPPLRVCQKETPMPYSKTLETATLPNVNRIL
DAIEKIMR
>Mature_327_residues
PNFVTLEIREAIRQAIDEEMTRDPNVCILGEEVAEYNGAYKVTKNLLDKWGPTRVIDTPISEAAFSGIGIGAALTGLRPI
IEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGANGAAAQVSCQHSHCVEALYANIPGLIIIAPSTPADAKGLL
KAAIRDNNPVLFLENELDYNLKGEVPTEEYLVPIGKAHIVQEGLDLTIISHSRMVTIVELAAKIAKQRWGFSIEILDLRT
IKPLDIAAILTSVKKTGNCLVVEEGHYFCGISAEIIATITEHIFDHLDHPPLRVCQKETPMPYSKTLETATLPNVNRILD
AIEKIMR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=332, Percent_Identity=50, Blast_Score=324, Evalue=7e-89,
Organism=Homo sapiens, GI291084858, Length=332, Percent_Identity=46.3855421686747, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=35.6037151702786, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=35.6037151702786, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17538422, Length=327, Percent_Identity=50.7645259938838, Blast_Score=325, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17506935, Length=321, Percent_Identity=35.202492211838, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=47.4006116207951, Blast_Score=316, Evalue=3e-87,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=49.5412844036697, Blast_Score=321, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=49.5412844036697, Blast_Score=321, Evalue=3e-88,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=35.2941176470588, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=35.2941176470588, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=57.3033707865169, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=57.3033707865169, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36050; Mature: 35919

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFVTLEIREAIRQAIDEEMTRDPNVCILGEEVAEYNGAYKVTKNLLDKWGPTRVIDTP
CCCEEEEHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHHHHHHHHHCCCCEEEECC
ISEAAFSGIGIGAALTGLRPIIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCEEEEEEEEEC
ANGAAAQVSCQHSHCVEALYANIPGLIIIAPSTPADAKGLLKAAIRDNNPVLFLENELDY
CCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCC
NLKGEVPTEEYLVPIGKAHIVQEGLDLTIISHSRMVTIVELAAKIAKQRWGFSIEILDLR
CCCCCCCCHHHEEECCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDIAAILTSVKKTGNCLVVEEGHYFCGISAEIIATITEHIFDHLDHPPLRVCQKET
CCCCHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PMPYSKTLETATLPNVNRILDAIEKIMR
CCCHHHCCCHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
PNFVTLEIREAIRQAIDEEMTRDPNVCILGEEVAEYNGAYKVTKNLLDKWGPTRVIDTP
CCEEEEHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHHHHHHHHHCCCCEEEECC
ISEAAFSGIGIGAALTGLRPIIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCEEEEEEEEEC
ANGAAAQVSCQHSHCVEALYANIPGLIIIAPSTPADAKGLLKAAIRDNNPVLFLENELDY
CCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCC
NLKGEVPTEEYLVPIGKAHIVQEGLDLTIISHSRMVTIVELAAKIAKQRWGFSIEILDLR
CCCCCCCCHHHEEECCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDIAAILTSVKKTGNCLVVEEGHYFCGISAEIIATITEHIFDHLDHPPLRVCQKET
CCCCHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PMPYSKTLETATLPNVNRILDAIEKIMR
CCCHHHCCCHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]