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Identifier: 15835136

GI number: 15835136

Start: 625738

End: 627024

Strand: Direct

Name: Not Available

Synonym: TC0518

Alternate gene names: 15835136

Gene position: 625738-627024 (Clockwise)

Preceding gene: 15835135

Following gene: 15835138

Centisome position: 58.32

GC content: 42.35

Gene sequence:

>1287_bases
GTGGTTTCTTTGTTAAAAATGCCTAAGCTATCCCCTACAATGGAAACAGGGACCCTTGTTAAATGGCATAAACAAGCTGG
TGATGAAGTCCATTTTGGAGATGTGTTATTAGAAATTTCTACTGATAAAGCCGTTTTAGAACATACGGCCTCTGAAGACG
GATGGTTGTTGCAAATTCTTGTGAAAGAAGGAACTAAAATTCCAATTGGCACACCTATCGCAGTGTTTTCAACAGAGCAA
AATGCCGAATATGATTTGAAACAGCTCCTGCCTTTAGAAGAGGCTTCAGGAGCAAATGAGCCTACAGAAATTTTACCTCA
AACTTCTCCTCAAAATGATTCACATTACTCGGGGCCTTCCATGGCTATTGTGGGATTCCGCCCCGAACCGCCTTTAACAA
CTCCTTTATCAGTTAAATACTCGGGAGATAAAGTAGCCGCCTCTCCCTTAGCTAAAAAATTAGCTAAGGAGCAGAACTTA
GATCTTTCTGGCGTAGCTGGTAGCGGACCAGGTGGACGGATTGTGAAAAAGGATTTGGAGAAAGCACCTCCTCTAAGAAT
AGCAGGTTTTGGATATCCTGAAGCCCCAGATGTTAATCCGGGCTCCTACGTAGAAGAGTCTCTATCCCCCATTAGAGAAT
CTATATCTAAACGATTACAAGCAGCCAAAACCTTTATTCCTCATTTTTATGTGCGGCAACGCATTTATGCTTCTCCTCTG
CTTGCGCTACTAAAAGAACTTCAAGTACAAAATATAAAACTTTCTATAAACGACTGCATCGTGCGAGCGTGCGCTTTAGC
CTTAAAAGAATTTCCAGAAATTAACTCTGGATTCAATAGCGTAGACAATACAATTATCCGATTTTCTACCATTGATATTT
CTATTGCTGTAGCAATTCCTGATGGAGTTATTACCCCTATTATCCGTTGTGCAGATAGAAAAAATGTTGGCACGATCTCA
GCCGAGATCAAAGGGTTAGCTGCAAGAGCAAGACAATTCTCTCTTAAAGAAGAGGAATACAAAGGCGGGTCTTTCTGTAT
CTCGAATCTTGGAATGACGGGGATTTCTGATTTTACGGCTATCCTAAACCCTCCTCAGGCAGCCATCCTAGCTGTAGGTA
GTGTAGAAGAGCAGCCTGTGGTCTTGAATGGAGAACTAGCTGTAGGATCAACTTGTATGCTAACTTTGTCAGTAGATCAC
CGAGTGATTGACGGGTACCCTGCAGCCATGTTCATGAAGAGACTGCAGAAACTTCTTGAGGCACCCTCCGTTTTGCTCCT
TAATTAG

Upstream 100 bases:

>100_bases
AAAGAAACTCCTATGCCATACAGTAAAACACTGGAGACGGCGACTCTTCCTAATGTTAACCGCATCCTGGATGCCATTGA
AAAAATTATGAGGTAACGTT

Downstream 100 bases:

>100_bases
AGATATGTCTCCCCCCTCTTCTTTTAAGAGGATTGGGAGACATTCCATATATTAGAAGCATAGTCTGTAATAGATCTATC
GCTTGAGAAGAAGCCTATTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVSLLKMPKLSPTMETGTLVKWHKQAGDEVHFGDVLLEISTDKAVLEHTASEDGWLLQILVKEGTKIPIGTPIAVFSTEQ
NAEYDLKQLLPLEEASGANEPTEILPQTSPQNDSHYSGPSMAIVGFRPEPPLTTPLSVKYSGDKVAASPLAKKLAKEQNL
DLSGVAGSGPGGRIVKKDLEKAPPLRIAGFGYPEAPDVNPGSYVEESLSPIRESISKRLQAAKTFIPHFYVRQRIYASPL
LALLKELQVQNIKLSINDCIVRACALALKEFPEINSGFNSVDNTIIRFSTIDISIAVAIPDGVITPIIRCADRKNVGTIS
AEIKGLAARARQFSLKEEEYKGGSFCISNLGMTGISDFTAILNPPQAAILAVGSVEEQPVVLNGELAVGSTCMLTLSVDH
RVIDGYPAAMFMKRLQKLLEAPSVLLLN

Sequences:

>Translated_428_residues
MVSLLKMPKLSPTMETGTLVKWHKQAGDEVHFGDVLLEISTDKAVLEHTASEDGWLLQILVKEGTKIPIGTPIAVFSTEQ
NAEYDLKQLLPLEEASGANEPTEILPQTSPQNDSHYSGPSMAIVGFRPEPPLTTPLSVKYSGDKVAASPLAKKLAKEQNL
DLSGVAGSGPGGRIVKKDLEKAPPLRIAGFGYPEAPDVNPGSYVEESLSPIRESISKRLQAAKTFIPHFYVRQRIYASPL
LALLKELQVQNIKLSINDCIVRACALALKEFPEINSGFNSVDNTIIRFSTIDISIAVAIPDGVITPIIRCADRKNVGTIS
AEIKGLAARARQFSLKEEEYKGGSFCISNLGMTGISDFTAILNPPQAAILAVGSVEEQPVVLNGELAVGSTCMLTLSVDH
RVIDGYPAAMFMKRLQKLLEAPSVLLLN
>Mature_428_residues
MVSLLKMPKLSPTMETGTLVKWHKQAGDEVHFGDVLLEISTDKAVLEHTASEDGWLLQILVKEGTKIPIGTPIAVFSTEQ
NAEYDLKQLLPLEEASGANEPTEILPQTSPQNDSHYSGPSMAIVGFRPEPPLTTPLSVKYSGDKVAASPLAKKLAKEQNL
DLSGVAGSGPGGRIVKKDLEKAPPLRIAGFGYPEAPDVNPGSYVEESLSPIRESISKRLQAAKTFIPHFYVRQRIYASPL
LALLKELQVQNIKLSINDCIVRACALALKEFPEINSGFNSVDNTIIRFSTIDISIAVAIPDGVITPIIRCADRKNVGTIS
AEIKGLAARARQFSLKEEEYKGGSFCISNLGMTGISDFTAILNPPQAAILAVGSVEEQPVVLNGELAVGSTCMLTLSVDH
RVIDGYPAAMFMKRLQKLLEAPSVLLLN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=37.7880184331797, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=33.9130434782609, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=33.695652173913, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=34.4978165938865, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI110671329, Length=453, Percent_Identity=25.3863134657837, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=41.875, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=30.8755760368664, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1786305, Length=297, Percent_Identity=28.6195286195286, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=40.5963302752294, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=28.978622327791, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=39.5454545454545, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=26.8235294117647, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=36.1233480176211, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=27.816091954023, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321632, Length=99, Percent_Identity=39.3939393939394, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129315, Length=293, Percent_Identity=38.2252559726962, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24582497, Length=293, Percent_Identity=38.2252559726962, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=25.9770114942529, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=30.3571428571429, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46178; Mature: 46178

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLLKMPKLSPTMETGTLVKWHKQAGDEVHFGDVLLEISTDKAVLEHTASEDGWLLQIL
CCCCCCCCCCCCCCCCCCEEEEHHHCCCCEEECEEEEEECCCHHHHHHCCCCCCEEEEEE
VKEGTKIPIGTPIAVFSTEQNAEYDLKQLLPLEEASGANEPTEILPQTSPQNDSHYSGPS
ECCCCCCCCCCCEEEEECCCCCCCCHHHHCCCHHCCCCCCCHHHCCCCCCCCCCCCCCCC
MAIVGFRPEPPLTTPLSVKYSGDKVAASPLAKKLAKEQNLDLSGVAGSGPGGRIVKKDLE
EEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHH
KAPPLRIAGFGYPEAPDVNPGSYVEESLSPIRESISKRLQAAKTFIPHFYVRQRIYASPL
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLKELQVQNIKLSINDCIVRACALALKEFPEINSGFNSVDNTIIRFSTIDISIAVAIP
HHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEEEEEEEEEEECC
DGVITPIIRCADRKNVGTISAEIKGLAARARQFSLKEEEYKGGSFCISNLGMTGISDFTA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEHHHCCCCCHHHHHH
ILNPPQAAILAVGSVEEQPVVLNGELAVGSTCMLTLSVDHRVIDGYPAAMFMKRLQKLLE
HCCCCHHEEEEECCCCCCCEEEECCEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHHC
APSVLLLN
CCCEEEEC
>Mature Secondary Structure
MVSLLKMPKLSPTMETGTLVKWHKQAGDEVHFGDVLLEISTDKAVLEHTASEDGWLLQIL
CCCCCCCCCCCCCCCCCCEEEEHHHCCCCEEECEEEEEECCCHHHHHHCCCCCCEEEEEE
VKEGTKIPIGTPIAVFSTEQNAEYDLKQLLPLEEASGANEPTEILPQTSPQNDSHYSGPS
ECCCCCCCCCCCEEEEECCCCCCCCHHHHCCCHHCCCCCCCHHHCCCCCCCCCCCCCCCC
MAIVGFRPEPPLTTPLSVKYSGDKVAASPLAKKLAKEQNLDLSGVAGSGPGGRIVKKDLE
EEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHH
KAPPLRIAGFGYPEAPDVNPGSYVEESLSPIRESISKRLQAAKTFIPHFYVRQRIYASPL
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLKELQVQNIKLSINDCIVRACALALKEFPEINSGFNSVDNTIIRFSTIDISIAVAIP
HHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEEEEEEEEEEECC
DGVITPIIRCADRKNVGTISAEIKGLAARARQFSLKEEEYKGGSFCISNLGMTGISDFTA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEHHHCCCCCHHHHHH
ILNPPQAAILAVGSVEEQPVVLNGELAVGSTCMLTLSVDHRVIDGYPAAMFMKRLQKLLE
HCCCCHHEEEEECCCCCCCEEEECCEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHHC
APSVLLLN
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]