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The map label for this gene is phoB [C]

Identifier: 158338366

GI number: 158338366

Start: 5332030

End: 5332737

Strand: Reverse

Name: phoB [C]

Synonym: AM1_5262

Alternate gene names: 158338366

Gene position: 5332737-5332030 (Counterclockwise)

Preceding gene: 158338367

Following gene: 158338365

Centisome position: 82.0

GC content: 48.59

Gene sequence:

>708_bases
ATGGGTCAGATTCTGTTAGTGGAAGATGAAGAAAATATTCGGGAAACTATTGCTCCCGCACTACAAAGTGAGGGATATCG
GGTAATCACGGCTGCGGATGGCCAAACAGCCCTGGATGTCATTCAGCTTTTTAAGTCGCGATCGCAGCAGGCATCCACCC
TAGACCTGATTATTTTAGATATTATGCTGCCTGGCATTAATGGCATTGATCTGTGCCGGTTTATTCGTCGGGAAGGCTCG
ACGGTTCCGGTACTGATGTTGAGTGCCAAGGATACGGAAGCCGATCGGGTTGTCGGTCTGGAAGTGGGTGCTGACGACTA
TCTCACGAAGCCCTTTGGCATGCGGGAACTGATCGCTCGCTGTCGGGCTCTCTTAAGACGACAGCGGCTGAATAATACTG
ACCCTCAAGGCACCCTGCTCACCTGTCGTGAGATCAGTTTGCATCCTGATGAATGTCGGGTCATGGTGCGAGATGAAGAA
GTCAATCTATCGCCCAAAGAATTCAGGATTTTAGAATTATTTATGAGCTATCCCCGGCGAGTGTGGCCAAGGGAGCAGAT
TCTCGAAAAGATTTGGGGGCCAGACTATATCGGCGATAGCAAAACGGTCGATGTACATATTCGTTGGCTACGGGAAAAAA
TAGAACTTGACCCCAGTAATCCTGATTACTTAGTCACCATTAGAGGATTTGGCTATCGGTTTGGATAA

Upstream 100 bases:

>100_bases
GAAGATTCAATGTTAGAATCGGCCTATGGCCGTTTAAGTAAAATTCTGTATGCTATCTGCTGACCCGAATGCGCGTTCGT
TTGAGCAACTTCCGACCACA

Downstream 100 bases:

>100_bases
GAGCGGTTGGAGGGCATCTCAAGCTTAAATAACGGAGAGTCTTGTGCAATTAGTATGGTTCCTTGCAGGATTAGGTCTTG
GTCTGGGACTGCTTGCTTGG

Product: two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGQILLVEDEENIRETIAPALQSEGYRVITAADGQTALDVIQLFKSRSQQASTLDLIILDIMLPGINGIDLCRFIRREGS
TVPVLMLSAKDTEADRVVGLEVGADDYLTKPFGMRELIARCRALLRRQRLNNTDPQGTLLTCREISLHPDECRVMVRDEE
VNLSPKEFRILELFMSYPRRVWPREQILEKIWGPDYIGDSKTVDVHIRWLREKIELDPSNPDYLVTIRGFGYRFG

Sequences:

>Translated_235_residues
MGQILLVEDEENIRETIAPALQSEGYRVITAADGQTALDVIQLFKSRSQQASTLDLIILDIMLPGINGIDLCRFIRREGS
TVPVLMLSAKDTEADRVVGLEVGADDYLTKPFGMRELIARCRALLRRQRLNNTDPQGTLLTCREISLHPDECRVMVRDEE
VNLSPKEFRILELFMSYPRRVWPREQILEKIWGPDYIGDSKTVDVHIRWLREKIELDPSNPDYLVTIRGFGYRFG
>Mature_234_residues
GQILLVEDEENIRETIAPALQSEGYRVITAADGQTALDVIQLFKSRSQQASTLDLIILDIMLPGINGIDLCRFIRREGST
VPVLMLSAKDTEADRVVGLEVGADDYLTKPFGMRELIARCRALLRRQRLNNTDPQGTLLTCREISLHPDECRVMVRDEEV
NLSPKEFRILELFMSYPRRVWPREQILEKIWGPDYIGDSKTVDVHIRWLREKIELDPSNPDYLVTIRGFGYRFG

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=238, Percent_Identity=41.5966386554622, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786911, Length=236, Percent_Identity=38.5593220338983, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789809, Length=237, Percent_Identity=34.1772151898734, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=38.0952380952381, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1786784, Length=230, Percent_Identity=35.6521739130435, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI87082012, Length=233, Percent_Identity=38.1974248927039, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=35.2941176470588, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788394, Length=238, Percent_Identity=34.453781512605, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789402, Length=233, Percent_Identity=35.6223175965665, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790552, Length=231, Percent_Identity=36.3636363636364, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI2367329, Length=236, Percent_Identity=34.3220338983051, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787229, Length=243, Percent_Identity=34.9794238683128, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787375, Length=237, Percent_Identity=29.1139240506329, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693140, Length=243, Percent_Identity=27.9835390946502, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787487, Length=123, Percent_Identity=37.3983739837398, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26961; Mature: 26830

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQILLVEDEENIRETIAPALQSEGYRVITAADGQTALDVIQLFKSRSQQASTLDLIILD
CCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHEEE
IMLPGINGIDLCRFIRREGSTVPVLMLSAKDTEADRVVGLEVGADDYLTKPFGMRELIAR
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCHHHCCCCCHHHHHHH
CRALLRRQRLNNTDPQGTLLTCREISLHPDECRVMVRDEEVNLSPKEFRILELFMSYPRR
HHHHHHHHHCCCCCCCCEEEEEEECCCCHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCC
VWPREQILEKIWGPDYIGDSKTVDVHIRWLREKIELDPSNPDYLVTIRGFGYRFG
CCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
GQILLVEDEENIRETIAPALQSEGYRVITAADGQTALDVIQLFKSRSQQASTLDLIILD
CCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHEEE
IMLPGINGIDLCRFIRREGSTVPVLMLSAKDTEADRVVGLEVGADDYLTKPFGMRELIAR
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCHHHCCCCCHHHHHHH
CRALLRRQRLNNTDPQGTLLTCREISLHPDECRVMVRDEEVNLSPKEFRILELFMSYPRR
HHHHHHHHHCCCCCCCCEEEEEEECCCCHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCC
VWPREQILEKIWGPDYIGDSKTVDVHIRWLREKIELDPSNPDYLVTIRGFGYRFG
CCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]