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The map label for this gene is sphS [H]

Identifier: 158338365

GI number: 158338365

Start: 5330595

End: 5331986

Strand: Reverse

Name: sphS [H]

Synonym: AM1_5261

Alternate gene names: 158338365

Gene position: 5331986-5330595 (Counterclockwise)

Preceding gene: 158338366

Following gene: 158338363

Centisome position: 81.98

GC content: 49.93

Gene sequence:

>1392_bases
GTGCAATTAGTATGGTTCCTTGCAGGATTAGGTCTTGGTCTGGGACTGCTTGCTTGGTCTGCTCGTCATTTCAATCACCA
ACTGGAACGAATCATTCGTACCCTGCCCAGTGATCCCTATCGGCCGACCCTGTCTCCCTTGAGTCGATTATCTCGGGTTA
CCCTGCAACTTCAAGATCAACTCCAGCTCAATGAACAAGAGCTGTTGGGATGGCAAGATATTCTGCGCCGAGCCCCGGTT
GGATATCTGCAGGTCAATGGTGCTGACAATTTGTATTGGATTAACGAACGAGCTTGCGATCTGTTGAAACTGCAAAGTCG
TTCTTGGGACGCTTGGCCCAACCGCCTCCTCCTAGAAGTTGTGCGTTCCATTGAGCTGGATCAATTAATTAGTCAGGTTC
GCTCAGGTCAGCAAGAGCATCAAATTGAGTGGATTATGCATCTGTCTGAGCGGGCTCAAGAGTGGCCCCTGAGAGCTTAT
GGTATTCCTTTGCCAGAAGGTCATGTAGGTGTGTTTATTGAGGATCGAACTGAAACCAAAAATTTGGCTGAGGATCGAGA
TCGCTGGACTTCAGATGTAGCCCATGAACTGAAGACCCCCCTTACGTCCATTCGCCTCATGGTGGAAACCCTTGAGCCCC
GCATTAGTTGGCAACATCGCTCCCATATTGATCGCTTATTGCAAGAGGTCACCCGCCTCAGCGACTTGGTGAAAGATTTA
CTTGAACTGAGTCGAATTACGTTCAATCAAGCCCAAACTTTGAAACTGCAACCCGTTAATGTCCCCAGTCTGATTCATAA
GGCTTGGCTGAATTTGGAACCTTTAGCTCAGCAGCGGAATCTATCTTTGTTTTACCAGGGGGTGGAAGACTGCACTTTAA
ACGTCGACAAGAATCGACTGTATCGGGTGTTCCTGAATCTGATTGACAATGCGATTAAGTACAGTCCGGTGGATCAGCCC
ATTATTGTCCAGGTAGAAGTCGTTCAGTCCGCGGAAGAGGGTGAACGGGGGCAACGCCTATGTATCGATATTATTGATAC
GGGGTCAGGTTTTTCGGCAGAGTCCCTGCCCCATATTTTTAAGCGTTTCTATCGTTCTGATCCCTCCAGAGTGAGAGGGA
CTGTGCCGATGGGAAATCCTGAGGCAGTGAGTTTGCAACCTCTAGGCACGTCAGATGTTATTCAAGCGACTTCTGTCGCC
CTATCAGAACAGGAGCAACCTTCCGCTGTGGCTCCTTTGGGAGGGAGTGGTTTAGGACTTGCGATCGCACAGCAAATCAT
CCTGGCCCATGGTGGCACCATTCGGGCCCGCAACCATCCCCAAACTGGCGGAGCCTGGCTACAAATTATGTTGCCTTGCC
CCCGTTGTTCCATGGCGCCAGAGGTTTCTTAG

Upstream 100 bases:

>100_bases
CCCAGTAATCCTGATTACTTAGTCACCATTAGAGGATTTGGCTATCGGTTTGGATAAGAGCGGTTGGAGGGCATCTCAAG
CTTAAATAACGGAGAGTCTT

Downstream 100 bases:

>100_bases
TTTTCTTACCTATAAGTTTTGAGTATCGATCTCAACCACAACGGGAGCATGATCGCTCGGTTTGGGTAACCGACGAGGGG
CTGCGTCAATTGTGCAAGCC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MQLVWFLAGLGLGLGLLAWSARHFNHQLERIIRTLPSDPYRPTLSPLSRLSRVTLQLQDQLQLNEQELLGWQDILRRAPV
GYLQVNGADNLYWINERACDLLKLQSRSWDAWPNRLLLEVVRSIELDQLISQVRSGQQEHQIEWIMHLSERAQEWPLRAY
GIPLPEGHVGVFIEDRTETKNLAEDRDRWTSDVAHELKTPLTSIRLMVETLEPRISWQHRSHIDRLLQEVTRLSDLVKDL
LELSRITFNQAQTLKLQPVNVPSLIHKAWLNLEPLAQQRNLSLFYQGVEDCTLNVDKNRLYRVFLNLIDNAIKYSPVDQP
IIVQVEVVQSAEEGERGQRLCIDIIDTGSGFSAESLPHIFKRFYRSDPSRVRGTVPMGNPEAVSLQPLGTSDVIQATSVA
LSEQEQPSAVAPLGGSGLGLAIAQQIILAHGGTIRARNHPQTGGAWLQIMLPCPRCSMAPEVS

Sequences:

>Translated_463_residues
MQLVWFLAGLGLGLGLLAWSARHFNHQLERIIRTLPSDPYRPTLSPLSRLSRVTLQLQDQLQLNEQELLGWQDILRRAPV
GYLQVNGADNLYWINERACDLLKLQSRSWDAWPNRLLLEVVRSIELDQLISQVRSGQQEHQIEWIMHLSERAQEWPLRAY
GIPLPEGHVGVFIEDRTETKNLAEDRDRWTSDVAHELKTPLTSIRLMVETLEPRISWQHRSHIDRLLQEVTRLSDLVKDL
LELSRITFNQAQTLKLQPVNVPSLIHKAWLNLEPLAQQRNLSLFYQGVEDCTLNVDKNRLYRVFLNLIDNAIKYSPVDQP
IIVQVEVVQSAEEGERGQRLCIDIIDTGSGFSAESLPHIFKRFYRSDPSRVRGTVPMGNPEAVSLQPLGTSDVIQATSVA
LSEQEQPSAVAPLGGSGLGLAIAQQIILAHGGTIRARNHPQTGGAWLQIMLPCPRCSMAPEVS
>Mature_463_residues
MQLVWFLAGLGLGLGLLAWSARHFNHQLERIIRTLPSDPYRPTLSPLSRLSRVTLQLQDQLQLNEQELLGWQDILRRAPV
GYLQVNGADNLYWINERACDLLKLQSRSWDAWPNRLLLEVVRSIELDQLISQVRSGQQEHQIEWIMHLSERAQEWPLRAY
GIPLPEGHVGVFIEDRTETKNLAEDRDRWTSDVAHELKTPLTSIRLMVETLEPRISWQHRSHIDRLLQEVTRLSDLVKDL
LELSRITFNQAQTLKLQPVNVPSLIHKAWLNLEPLAQQRNLSLFYQGVEDCTLNVDKNRLYRVFLNLIDNAIKYSPVDQP
IIVQVEVVQSAEEGERGQRLCIDIIDTGSGFSAESLPHIFKRFYRSDPSRVRGTVPMGNPEAVSLQPLGTSDVIQATSVA
LSEQEQPSAVAPLGGSGLGLAIAQQIILAHGGTIRARNHPQTGGAWLQIMLPCPRCSMAPEVS

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=298, Percent_Identity=27.5167785234899, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786783, Length=267, Percent_Identity=26.5917602996255, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=284, Percent_Identity=27.112676056338, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786600, Length=271, Percent_Identity=23.2472324723247, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52438; Mature: 52438

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVWFLAGLGLGLGLLAWSARHFNHQLERIIRTLPSDPYRPTLSPLSRLSRVTLQLQDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LQLNEQELLGWQDILRRAPVGYLQVNGADNLYWINERACDLLKLQSRSWDAWPNRLLLEV
HCCCHHHHCCHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHH
VRSIELDQLISQVRSGQQEHQIEWIMHLSERAQEWPLRAYGIPLPEGHVGVFIEDRTETK
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCHH
NLAEDRDRWTSDVAHELKTPLTSIRLMVETLEPRISWQHRSHIDRLLQEVTRLSDLVKDL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
LELSRITFNQAQTLKLQPVNVPSLIHKAWLNLEPLAQQRNLSLFYQGVEDCTLNVDKNRL
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHCCCHHEECCCCCCEEECCHHHH
YRVFLNLIDNAIKYSPVDQPIIVQVEVVQSAEEGERGQRLCIDIIDTGSGFSAESLPHIF
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHCHHCCCCCCEEEEEEEECCCCCCHHHHHHHH
KRFYRSDPSRVRGTVPMGNPEAVSLQPLGTSDVIQATSVALSEQEQPSAVAPLGGSGLGL
HHHHCCCHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AIAQQIILAHGGTIRARNHPQTGGAWLQIMLPCPRCSMAPEVS
HHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQLVWFLAGLGLGLGLLAWSARHFNHQLERIIRTLPSDPYRPTLSPLSRLSRVTLQLQDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LQLNEQELLGWQDILRRAPVGYLQVNGADNLYWINERACDLLKLQSRSWDAWPNRLLLEV
HCCCHHHHCCHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHH
VRSIELDQLISQVRSGQQEHQIEWIMHLSERAQEWPLRAYGIPLPEGHVGVFIEDRTETK
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCHH
NLAEDRDRWTSDVAHELKTPLTSIRLMVETLEPRISWQHRSHIDRLLQEVTRLSDLVKDL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHH
LELSRITFNQAQTLKLQPVNVPSLIHKAWLNLEPLAQQRNLSLFYQGVEDCTLNVDKNRL
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHCCCHHEECCCCCCEEECCHHHH
YRVFLNLIDNAIKYSPVDQPIIVQVEVVQSAEEGERGQRLCIDIIDTGSGFSAESLPHIF
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHCHHCCCCCCEEEEEEEECCCCCCHHHHHHHH
KRFYRSDPSRVRGTVPMGNPEAVSLQPLGTSDVIQATSVALSEQEQPSAVAPLGGSGLGL
HHHHCCCHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AIAQQIILAHGGTIRARNHPQTGGAWLQIMLPCPRCSMAPEVS
HHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]