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The map label for this gene is amyB [H]

Identifier: 158338201

GI number: 158338201

Start: 5148528

End: 5150342

Strand: Direct

Name: amyB [H]

Synonym: AM1_5095

Alternate gene names: 158338201

Gene position: 5148528-5150342 (Clockwise)

Preceding gene: 158338200

Following gene: 158338202

Centisome position: 79.16

GC content: 48.54

Gene sequence:

>1815_bases
ATGTTTAGTGCTCCTCAGCATGCAGTGGCTGTCGCTTGTCTAACTTCAGTGATCGCCGTTGCCCCTGTTCCTATGCGCTC
TGCCTTTGCTCAGTCCCAATTTACGGACGTCAGTAGCAATTGGGCTCAACCTTTCATTGATACCCTTGCTCAAAAAAATA
TAATTAGTGGTTTTCCCGATGGCTCCTTCCGACCTGACCAACCCGTGACTCGGGCTCAGTTTGCTGCCATTGTCCGCCAA
GCTTTTAAGTCTCCAGCTACTCGCCTTTCACGCCCTTTTAAGGATGTAAGCTATGCTTACTGGGCTTCTCCAGCCATTGA
CTATGCTTACACCACAGGGTTCTTGTCGGGCTATACCAACGGATCCTTCCAGCCAGATCAAGAGATCCCTAAGGTACAAG
CGTTGGTGGCCATTGCCAGTGGATTAAAGATTCAACCTGTGGGGTCTGTGGATAGCACCTTGCTCCGCTTCTACGATGCA
GCTCAAATTCCCAACTATGCTAAACCGGGGGTTGCTGCGGCAACGCAACGTAATATTCCTGTGAATTATCCAAATGCGGC
TACCTTAAATCCGAATCAACAAGCCACTCGGGCCGATATTGCTGCCTATATTTATCAAGCATTAGTTAGTCAGGGACGCT
TGGCTCCCATTGGCAGTAATTTCCCTGCCTCACAATATATTGTTCAGGGCGGCTCTCTCCCGGCGACACCCTCAGAAGCT
GGTACTGGAACTTTAGCGGTGGGGACTGAGCTTCCCGTTCAGTTAGAAGGAGCAGAACCCGGTGCCAATCTCGTGATGAC
CGTGGGCGAAAATGTTGAAACCACGTTTGTCGTTGCGGAAGATGTGGTGAATACCCGTCAACAGGTTGTTATCCCTGAAG
GCAGTAAGATTGTCGGTCGTTTCCAGCCCTTTGAAGTAAACAAGACCTTAGGGACTCAGTTCACGGCCAGTAAATTAATG
ATTGGCAATACGCCCTATACCGTTAATGCGTCTTCTGTTCCCATTGCTGCTCGCAAAAAGAGTTCTCTAAGTATTGCTGA
TTTAACCGGAAGTATCACCACTCTGGCCGCTTCAGCCGCTTTGGATTCTGCTATTAGTGGGAATGTGCGATTTGGTTCCT
TAGTGCCTGGGATTATCCAGACTGGGCAAACCATAGGCAAACAGGTGATCGGCAAATCCGATACCAGTGATGAGGTCATT
CTTGTTGAACCCGATCTGATGGGATTAAAGCTCAATGCTGATTTTGAGTTTTTACCGGGTTCAACCACAGCCTTTGTGGA
TCCTAATTCTGCCAATCTATATCAGGTGGACTCTGGGACAAAGGTCGATCTGGCAGCCCAAACAGACAATGCCAGATACG
TCATGGTTCCCGGTGAAACGGTTCCCGTGGAGCTAAAAACGGGACGTTCTATCTATAACCGTCAAAATGAAGTTCTTGTG
CCAGAGGGGAGTCTGATTCGTGGCCAGATCGTACCGATGACGGTGAATGGCAAAGAAGGAGCCCAATTTGTGGCTAATGA
AATTGTGATTGGCACCCAAACATATCCCATTACTGCTGCTTCAGCAGGGATTTCTCCCTCTTCCTTGCAGTCTTTAAGCC
CAGCTGATTTTCAAGGGAATGTGATTGCTTCTCCTGAAGCCAGTCAATCTCTTCGCGGGGTTAGCTCAACTGGCGAGCAA
CAGTCAGGTGGCTTATTAGGCCTAGGAAGTCTGATTGGTGGAAGCGGTTCCGGTAAAAAGGTGATCTTATTTAACCCCAC
CTCTGTACAGATGGAGTTTCGTGCTCCCTTGAGCTTAAATAACTTCAATAACTAG

Upstream 100 bases:

>100_bases
CCCTGCATCACCTTTCGTCAAAACGGGAATGGGATTTTATTGACATGGGGAATACACAGTTCAACCTGTTCAATCCAATC
CTAAAAAAGGTGTTAGAAAC

Downstream 100 bases:

>100_bases
AGATAATGGGCTGCCCAAGTTGCCTCGATCAGAGGCAATCCCGGTAGGGGCTTGGGCGGTTACCTTGTCAAGATTTTCAT
AATTGAAGATTGGCTTTGTC

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Pullulanase type II; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MFSAPQHAVAVACLTSVIAVAPVPMRSAFAQSQFTDVSSNWAQPFIDTLAQKNIISGFPDGSFRPDQPVTRAQFAAIVRQ
AFKSPATRLSRPFKDVSYAYWASPAIDYAYTTGFLSGYTNGSFQPDQEIPKVQALVAIASGLKIQPVGSVDSTLLRFYDA
AQIPNYAKPGVAAATQRNIPVNYPNAATLNPNQQATRADIAAYIYQALVSQGRLAPIGSNFPASQYIVQGGSLPATPSEA
GTGTLAVGTELPVQLEGAEPGANLVMTVGENVETTFVVAEDVVNTRQQVVIPEGSKIVGRFQPFEVNKTLGTQFTASKLM
IGNTPYTVNASSVPIAARKKSSLSIADLTGSITTLAASAALDSAISGNVRFGSLVPGIIQTGQTIGKQVIGKSDTSDEVI
LVEPDLMGLKLNADFEFLPGSTTAFVDPNSANLYQVDSGTKVDLAAQTDNARYVMVPGETVPVELKTGRSIYNRQNEVLV
PEGSLIRGQIVPMTVNGKEGAQFVANEIVIGTQTYPITAASAGISPSSLQSLSPADFQGNVIASPEASQSLRGVSSTGEQ
QSGGLLGLGSLIGGSGSGKKVILFNPTSVQMEFRAPLSLNNFNN

Sequences:

>Translated_604_residues
MFSAPQHAVAVACLTSVIAVAPVPMRSAFAQSQFTDVSSNWAQPFIDTLAQKNIISGFPDGSFRPDQPVTRAQFAAIVRQ
AFKSPATRLSRPFKDVSYAYWASPAIDYAYTTGFLSGYTNGSFQPDQEIPKVQALVAIASGLKIQPVGSVDSTLLRFYDA
AQIPNYAKPGVAAATQRNIPVNYPNAATLNPNQQATRADIAAYIYQALVSQGRLAPIGSNFPASQYIVQGGSLPATPSEA
GTGTLAVGTELPVQLEGAEPGANLVMTVGENVETTFVVAEDVVNTRQQVVIPEGSKIVGRFQPFEVNKTLGTQFTASKLM
IGNTPYTVNASSVPIAARKKSSLSIADLTGSITTLAASAALDSAISGNVRFGSLVPGIIQTGQTIGKQVIGKSDTSDEVI
LVEPDLMGLKLNADFEFLPGSTTAFVDPNSANLYQVDSGTKVDLAAQTDNARYVMVPGETVPVELKTGRSIYNRQNEVLV
PEGSLIRGQIVPMTVNGKEGAQFVANEIVIGTQTYPITAASAGISPSSLQSLSPADFQGNVIASPEASQSLRGVSSTGEQ
QSGGLLGLGSLIGGSGSGKKVILFNPTSVQMEFRAPLSLNNFNN
>Mature_604_residues
MFSAPQHAVAVACLTSVIAVAPVPMRSAFAQSQFTDVSSNWAQPFIDTLAQKNIISGFPDGSFRPDQPVTRAQFAAIVRQ
AFKSPATRLSRPFKDVSYAYWASPAIDYAYTTGFLSGYTNGSFQPDQEIPKVQALVAIASGLKIQPVGSVDSTLLRFYDA
AQIPNYAKPGVAAATQRNIPVNYPNAATLNPNQQATRADIAAYIYQALVSQGRLAPIGSNFPASQYIVQGGSLPATPSEA
GTGTLAVGTELPVQLEGAEPGANLVMTVGENVETTFVVAEDVVNTRQQVVIPEGSKIVGRFQPFEVNKTLGTQFTASKLM
IGNTPYTVNASSVPIAARKKSSLSIADLTGSITTLAASAALDSAISGNVRFGSLVPGIIQTGQTIGKQVIGKSDTSDEVI
LVEPDLMGLKLNADFEFLPGSTTAFVDPNSANLYQVDSGTKVDLAAQTDNARYVMVPGETVPVELKTGRSIYNRQNEVLV
PEGSLIRGQIVPMTVNGKEGAQFVANEIVIGTQTYPITAASAGISPSSLQSLSPADFQGNVIASPEASQSLRGVSSTGEQ
QSGGLLGLGSLIGGSGSGKKVILFNPTSVQMEFRAPLSLNNFNN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted, cell wall. Note=It C-terminus may serve as an S-layer anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR001119 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00395 SLH [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 63338; Mature: 63338

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSAPQHAVAVACLTSVIAVAPVPMRSAFAQSQFTDVSSNWAQPFIDTLAQKNIISGFPD
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
GSFRPDQPVTRAQFAAIVRQAFKSPATRLSRPFKDVSYAYWASPAIDYAYTTGFLSGYTN
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHCCCC
GSFQPDQEIPKVQALVAIASGLKIQPVGSVDSTLLRFYDAAQIPNYAKPGVAAATQRNIP
CCCCCCHHCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCC
VNYPNAATLNPNQQATRADIAAYIYQALVSQGRLAPIGSNFPASQYIVQGGSLPATPSEA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
GTGTLAVGTELPVQLEGAEPGANLVMTVGENVETTFVVAEDVVNTRQQVVIPEGSKIVGR
CCCEEEECCCCCEEEECCCCCCCEEEEECCCCEEEEEEEHHHHCCCCEEEECCCCEEEEE
FQPFEVNKTLGTQFTASKLMIGNTPYTVNASSVPIAARKKSSLSIADLTGSITTLAASAA
CCCEEECHHHCCEEEECEEEECCCCEEECCCCCCEEECCCCCCEEECCCCCHHHHHHHHH
LDSAISGNVRFGSLVPGIIQTGQTIGKQVIGKSDTSDEVILVEPDLMGLKLNADFEFLPG
HHHHHCCCEEECCCCHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEECCC
STTAFVDPNSANLYQVDSGTKVDLAAQTDNARYVMVPGETVPVELKTGRSIYNRQNEVLV
CEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEECCCEEEEEEECCCHHHCCCCCEEC
PEGSLIRGQIVPMTVNGKEGAQFVANEIVIGTQTYPITAASAGISPSSLQSLSPADFQGN
CCCCEEECEEEEEEECCCCCHHEEEEEEEECCCCCCEEECCCCCCHHHHHCCCCCCCCCC
VIASPEASQSLRGVSSTGEQQSGGLLGLGSLIGGSGSGKKVILFNPTSVQMEFRAPLSLN
EEECCCHHHHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCEEEEECCCEEEEEEECCCCCC
NFNN
CCCC
>Mature Secondary Structure
MFSAPQHAVAVACLTSVIAVAPVPMRSAFAQSQFTDVSSNWAQPFIDTLAQKNIISGFPD
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
GSFRPDQPVTRAQFAAIVRQAFKSPATRLSRPFKDVSYAYWASPAIDYAYTTGFLSGYTN
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHCCCC
GSFQPDQEIPKVQALVAIASGLKIQPVGSVDSTLLRFYDAAQIPNYAKPGVAAATQRNIP
CCCCCCHHCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCC
VNYPNAATLNPNQQATRADIAAYIYQALVSQGRLAPIGSNFPASQYIVQGGSLPATPSEA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
GTGTLAVGTELPVQLEGAEPGANLVMTVGENVETTFVVAEDVVNTRQQVVIPEGSKIVGR
CCCEEEECCCCCEEEECCCCCCCEEEEECCCCEEEEEEEHHHHCCCCEEEECCCCEEEEE
FQPFEVNKTLGTQFTASKLMIGNTPYTVNASSVPIAARKKSSLSIADLTGSITTLAASAA
CCCEEECHHHCCEEEECEEEECCCCEEECCCCCCEEECCCCCCEEECCCCCHHHHHHHHH
LDSAISGNVRFGSLVPGIIQTGQTIGKQVIGKSDTSDEVILVEPDLMGLKLNADFEFLPG
HHHHHCCCEEECCCCHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEECCC
STTAFVDPNSANLYQVDSGTKVDLAAQTDNARYVMVPGETVPVELKTGRSIYNRQNEVLV
CEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEECCCEEEEEEECCCHHHCCCCCEEC
PEGSLIRGQIVPMTVNGKEGAQFVANEIVIGTQTYPITAASAGISPSSLQSLSPADFQGN
CCCCEEECEEEEEEECCCCCHHEEEEEEEECCCCCCEEECCCCCCHHHHHCCCCCCCCCC
VIASPEASQSLRGVSSTGEQQSGGLLGLGSLIGGSGSGKKVILFNPTSVQMEFRAPLSLN
EEECCCHHHHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCEEEEECCCEEEEEEECCCCCC
NFNN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8195085 [H]