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The map label for this gene is pntB [H]

Identifier: 158337469

GI number: 158337469

Start: 4377641

End: 4379065

Strand: Direct

Name: pntB [H]

Synonym: AM1_4348

Alternate gene names: 158337469

Gene position: 4377641-4379065 (Clockwise)

Preceding gene: 158337468

Following gene: 158337470

Centisome position: 67.31

GC content: 52.91

Gene sequence:

>1425_bases
ATGACCAATAGCCTTGTCACCGTGGCTTACATCGCGGCAAGTGCATTATTTATCTTGAGTTTGGCCGGTTTGTCGAATCA
GGAAACCGCTCGCAAGGGCAATGTTTACGGCATTCTCGGCATGGTGATTGCCTTAGGCGCAACGGCCCTCAGTGCCGAAG
TGACTAGCTATAGCACCCTGGCTGCCGTGGTCATTCCCGGTGGCATTATTGGCGCGATTGTGGCGTCGCGTGTAGCCATG
ACCTCCATGCCTGAATTGGTGGCTATCTTACATAGCTTTGTCGGCTTAGCTGCGGTTCTAGTCGGCATTGCCAACTACCT
GCAACCAGAATCAGGCTTATCTGGTGGTGAAGAAGCCATTCACCATATCGAAATTTACCTGGGCGTCTTTATTGGCGCGG
TTACCTTTACCGGGTCGATTATTGCCTTTGCCAAACTGCGAGCCATTATTGGCAGTAAGCCGTTGATGTTGCCCGCCCGC
CATTTCCTGAATATTGGCATGTTGGTGGGTTCGGTGATTCTGGGTGCTCAGTTTATGGGTGCGGCAGGTACCAGTGGTTT
GCAACCCTTACTGATCATGACTGCGATCGCAAGTCTGTTGGGTATCCATTTGGTGGCGGCAATCGGCGGTGCAGATATGC
CTGTGGTGATCTCCATGCTCAACAGCTATTCCGGCTGGGCGGCTGCTGCAGCTGGCTTTATGTTGGGGAATGACCTCTTG
ATTATCACCGGTGCCCTAGTGGGCAGTAGCGGCGCTATCCTCAGCTACATCATGTGTAAAGCCATGAACCGAGGGTTCTT
CAGCGTGATTCTGGGTGGATTTGGCGCAGCACCGAAAGGCGGCTCCAAGTCAGCGGATGGTCCAGTTGGTGAAGCCACCT
CCACCACAGTTGATGACACAGTGGAATTGCTCAAAAATGCCAAGAACGTGATTATTGCCCCAGGCTATGGCATGGCCGTG
GCTCAGGCTCAGCATCCCGTGGCTCAAATCACTAAGATCCTGCGGGATCAGGGCACCAATGTCCGCTTTGGTATTCACCC
GGTCGCAGGTCGGCTACCGGGTCATATGAACGTGCTGCTGGCAGAAGCCAATGTGCCTTACGATATCGTGCTGGAAATGG
ACGAAATTAACGATGATTTCGCCGAAACCGATCTCGTGCTGGTGATTGGGGCTAATGATACGGTTAATCCCAGCGCCCTC
AGTGATCCTGAAAGTCCCATAGCCGGTATGCCCGTGCTAGAAGTCTGGAATGCTGAGAATGTTGTGGTGATGAAGCGAGG
GATGTCCAGCGGGTATGCAGGGGTTGACAATCCCTTGTTCTACAATGACAACACCGAAATGCTGTTTGGAGATGCTCGTC
AAAACGTTGATGCTATCTTAGGGCAACTCGCTGCTACCAAGACCAAGACAGCCGTCGCCGCTTAA

Upstream 100 bases:

>100_bases
TGCAAATGTTCCGCCGTTAGTCTCTGTTCTATTTTCCACACCTCTTTCCGCTGTCCTCAACTGGCTAGGACAGCGACTAT
CCCTTTTTTAGGAGTCCACT

Downstream 100 bases:

>100_bases
CGGCTCTGACTCAATATTCTGCCTAAAGTAGAGAATGCCTGTTTCTATTAGGTGTTCTCTACTTTTTTTATGGATAAACC
TCTGCTTTTGACACAAAACC

Product: NAD(P) transhydrogenase beta subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTNSLVTVAYIAASALFILSLAGLSNQETARKGNVYGILGMVIALGATALSAEVTSYSTLAAVVIPGGIIGAIVASRVAM
TSMPELVAILHSFVGLAAVLVGIANYLQPESGLSGGEEAIHHIEIYLGVFIGAVTFTGSIIAFAKLRAIIGSKPLMLPAR
HFLNIGMLVGSVILGAQFMGAAGTSGLQPLLIMTAIASLLGIHLVAAIGGADMPVVISMLNSYSGWAAAAAGFMLGNDLL
IITGALVGSSGAILSYIMCKAMNRGFFSVILGGFGAAPKGGSKSADGPVGEATSTTVDDTVELLKNAKNVIIAPGYGMAV
AQAQHPVAQITKILRDQGTNVRFGIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINDDFAETDLVLVIGANDTVNPSAL
SDPESPIAGMPVLEVWNAENVVVMKRGMSSGYAGVDNPLFYNDNTEMLFGDARQNVDAILGQLAATKTKTAVAA

Sequences:

>Translated_474_residues
MTNSLVTVAYIAASALFILSLAGLSNQETARKGNVYGILGMVIALGATALSAEVTSYSTLAAVVIPGGIIGAIVASRVAM
TSMPELVAILHSFVGLAAVLVGIANYLQPESGLSGGEEAIHHIEIYLGVFIGAVTFTGSIIAFAKLRAIIGSKPLMLPAR
HFLNIGMLVGSVILGAQFMGAAGTSGLQPLLIMTAIASLLGIHLVAAIGGADMPVVISMLNSYSGWAAAAAGFMLGNDLL
IITGALVGSSGAILSYIMCKAMNRGFFSVILGGFGAAPKGGSKSADGPVGEATSTTVDDTVELLKNAKNVIIAPGYGMAV
AQAQHPVAQITKILRDQGTNVRFGIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINDDFAETDLVLVIGANDTVNPSAL
SDPESPIAGMPVLEVWNAENVVVMKRGMSSGYAGVDNPLFYNDNTEMLFGDARQNVDAILGQLAATKTKTAVAA
>Mature_473_residues
TNSLVTVAYIAASALFILSLAGLSNQETARKGNVYGILGMVIALGATALSAEVTSYSTLAAVVIPGGIIGAIVASRVAMT
SMPELVAILHSFVGLAAVLVGIANYLQPESGLSGGEEAIHHIEIYLGVFIGAVTFTGSIIAFAKLRAIIGSKPLMLPARH
FLNIGMLVGSVILGAQFMGAAGTSGLQPLLIMTAIASLLGIHLVAAIGGADMPVVISMLNSYSGWAAAAAGFMLGNDLLI
ITGALVGSSGAILSYIMCKAMNRGFFSVILGGFGAAPKGGSKSADGPVGEATSTTVDDTVELLKNAKNVIIAPGYGMAVA
QAQHPVAQITKILRDQGTNVRFGIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINDDFAETDLVLVIGANDTVNPSALS
DPESPIAGMPVLEVWNAENVVVMKRGMSSGYAGVDNPLFYNDNTEMLFGDARQNVDAILGQLAATKTKTAVAA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:
GO:0005886: NAD(P) transhydrogenase subunit beta
GO:0008750: NAD(P) transhydrogenase subunit beta
GO:0016021: NAD(P) transhydrogenase subunit beta
GO:0016491: NAD(P) transhydrogenase subunit beta
GO:0050661: NAD(P) transhydrogenase subunit beta
GO:0055114: NAD(P) transhydrogenase subunit beta

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=466, Percent_Identity=51.0729613733906, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI122939153, Length=466, Percent_Identity=51.0729613733906, Blast_Score=454, Evalue=1e-128,
Organism=Escherichia coli, GI1787886, Length=465, Percent_Identity=66.6666666666667, Blast_Score=593, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17550456, Length=473, Percent_Identity=49.6828752642706, Blast_Score=434, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48651; Mature: 48520

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNSLVTVAYIAASALFILSLAGLSNQETARKGNVYGILGMVIALGATALSAEVTSYSTL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVVIPGGIIGAIVASRVAMTSMPELVAILHSFVGLAAVLVGIANYLQPESGLSGGEEAI
HHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HHIEIYLGVFIGAVTFTGSIIAFAKLRAIIGSKPLMLPARHFLNIGMLVGSVILGAQFMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
AAGTSGLQPLLIMTAIASLLGIHLVAAIGGADMPVVISMLNSYSGWAAAAAGFMLGNDLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEE
IITGALVGSSGAILSYIMCKAMNRGFFSVILGGFGAAPKGGSKSADGPVGEATSTTVDDT
EEEEHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VELLKNAKNVIIAPGYGMAVAQAQHPVAQITKILRDQGTNVRFGIHPVAGRLPGHMNVLL
HHHHHCCCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCEEEECCHHCCCCCCCEEEEE
AEANVPYDIVLEMDEINDDFAETDLVLVIGANDTVNPSALSDPESPIAGMPVLEVWNAEN
EECCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
VVVMKRGMSSGYAGVDNPLFYNDNTEMLFGDARQNVDAILGQLAATKTKTAVAA
EEEEECCCCCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]