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The map label for this gene is pntA [H]

Identifier: 158337468

GI number: 158337468

Start: 4375944

End: 4377560

Strand: Direct

Name: pntA [H]

Synonym: AM1_4347

Alternate gene names: 158337468

Gene position: 4375944-4377560 (Clockwise)

Preceding gene: 158337467

Following gene: 158337469

Centisome position: 67.28

GC content: 51.95

Gene sequence:

>1617_bases
ATGACTGTTGCCGACAAGAAATTAGCGAACGCTGATAACCCTCAAGATCCAAAAACAGGCCTAAAAATCGGCATCCCGAA
AGAAGTCTTTCCCGGCGAGCGCCGAGTCGCAGCGACCCCCGATACGGCTAAAACCCTGCAAAAAATGGGATTTGAAGTCT
TAGTGGAATCTAAAGCAGGCGCTGGTGCCACGTTTTCCGATCAGGTCTATCAACAGGCTGGCTGCCAAGTCGTGGCAGAT
ACCACTCAACTCTGGGAAACCGCCGATATTATTCTCAAGGTGCGGGCACCCCAGATGCACCCTACCCTGAATCGCCATGA
AGCAGAACTTTTAAATGAAGGCAAAACCCTAATCAGCTTTATTTGGCCTGCTCAAAACGAAGACTTATTGAAGCAGTTAG
CTGAGCAAAAAGCCAATGTTCTGGCGATGGATGCGATCCCTCGAATTAGCCGCGCTCAGAAAATGGATGCCCTGAGCTCC
ATGGCCAATATTGCCGGCTATCGGGCTGTGGTGGAATCGGCGAATAACTTTGGTCGCTTTTTTACTGGACAAATCACCGC
TGCGGGGAAAGTTCCTCCCGCTAAAGTGCTCGTGATTGGAGCTGGTGTTGCCGGTCTTGCCGCAATTGGTGCAGCTCGAG
GTTTAGGCGCTGTGGTTCGCTCCTTCGATACTCGACCTGTGGTCAAAGAACAGGTGCAAAGTATGGGGGCTGAATTCCTA
GAGCTGGAATTTGAGGAAGATGGCACCGGAGCAGGGGGCTATGCCAAAACCATGAGCCCTGAGTTTATTGCTGCTGAAAT
GGAGCTGTTTGCTCAGCAAGCTGAAGAAGTAGATATCATCATTACCACTGCCTTGATCCCAGGTAAGAAAGCGCCGTTGC
TGATTACCAAAGATATGGTGGAGAGCATGAAGGAAGGCTCTGTCGTCGTGGATATGGCAGCAGAGCAGGGGGGCAACTGC
GAAGTCACCCGACCGAACGAAGTTTATCAACATCATGGTGTGACGATTGTTGGTCTGACGGATTTGCCCAGCCGCATGGC
CAACCAATCAAGTCAGCTCTATGGCAACAACCTGTGCCATTTGCTTAAGGATATGGGTGGCGCTGAAGACTACAAAATTG
ACTTTGAAGACGAAGTGGTGCGAGGCGCAACCGTTCTGCAAGAGGGCAAAATCACTTGGCCCCCACCTCAAGTCAGTCAG
CCTTCTCCCCCTAAGCCTGCTGCCGAGGCTAAGCAACCCGCAATGCCCGCTGAGCCGGAAGTAAAAGAAAAATCGGTGAT
TGGCCAATGGCTCCCCTTGATTGCAGTGGGTGCAGCCTTATTTGGCATTGGCTTAACCGCTCCTCCTTCTTTCCTGACTC
ACTTCACCGTGTTTGTGTTGGCCTGTTTTGTGGGTTGGCAGGTGATTTGGAATGTGGCCCCTGCGTTGCATACGCCTTTG
ATGAGTGTCACTAACGCTATTAGCGGCATCATTATTCTCGGGGGCATGCTGCAAATCTCGGGTCCCTTGGGTTCACCGAC
CACGATACTGGGTGCGATCGCAATCCTAGTCGGCACCATCAACATCGCCGGGGGATTCCTCGTTACCCAACGGATGCTGC
AAATGTTCCGCCGTTAG

Upstream 100 bases:

>100_bases
AGTTTTTTGAAAACTATATCCCGCCTCTATATTCCTAGGGAGGTCAGACGCACCGTAATTTTTAGTTTCCGTTGAAGACT
TACCACCGCAAGGAGCATTC

Downstream 100 bases:

>100_bases
TCTCTGTTCTATTTTCCACACCTCTTTCCGCTGTCCTCAACTGGCTAGGACAGCGACTATCCCTTTTTTAGGAGTCCACT
ATGACCAATAGCCTTGTCAC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTVADKKLANADNPQDPKTGLKIGIPKEVFPGERRVAATPDTAKTLQKMGFEVLVESKAGAGATFSDQVYQQAGCQVVAD
TTQLWETADIILKVRAPQMHPTLNRHEAELLNEGKTLISFIWPAQNEDLLKQLAEQKANVLAMDAIPRISRAQKMDALSS
MANIAGYRAVVESANNFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAVVRSFDTRPVVKEQVQSMGAEFL
ELEFEEDGTGAGGYAKTMSPEFIAAEMELFAQQAEEVDIIITTALIPGKKAPLLITKDMVESMKEGSVVVDMAAEQGGNC
EVTRPNEVYQHHGVTIVGLTDLPSRMANQSSQLYGNNLCHLLKDMGGAEDYKIDFEDEVVRGATVLQEGKITWPPPQVSQ
PSPPKPAAEAKQPAMPAEPEVKEKSVIGQWLPLIAVGAALFGIGLTAPPSFLTHFTVFVLACFVGWQVIWNVAPALHTPL
MSVTNAISGIIILGGMLQISGPLGSPTTILGAIAILVGTINIAGGFLVTQRMLQMFRR

Sequences:

>Translated_538_residues
MTVADKKLANADNPQDPKTGLKIGIPKEVFPGERRVAATPDTAKTLQKMGFEVLVESKAGAGATFSDQVYQQAGCQVVAD
TTQLWETADIILKVRAPQMHPTLNRHEAELLNEGKTLISFIWPAQNEDLLKQLAEQKANVLAMDAIPRISRAQKMDALSS
MANIAGYRAVVESANNFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAVVRSFDTRPVVKEQVQSMGAEFL
ELEFEEDGTGAGGYAKTMSPEFIAAEMELFAQQAEEVDIIITTALIPGKKAPLLITKDMVESMKEGSVVVDMAAEQGGNC
EVTRPNEVYQHHGVTIVGLTDLPSRMANQSSQLYGNNLCHLLKDMGGAEDYKIDFEDEVVRGATVLQEGKITWPPPQVSQ
PSPPKPAAEAKQPAMPAEPEVKEKSVIGQWLPLIAVGAALFGIGLTAPPSFLTHFTVFVLACFVGWQVIWNVAPALHTPL
MSVTNAISGIIILGGMLQISGPLGSPTTILGAIAILVGTINIAGGFLVTQRMLQMFRR
>Mature_537_residues
TVADKKLANADNPQDPKTGLKIGIPKEVFPGERRVAATPDTAKTLQKMGFEVLVESKAGAGATFSDQVYQQAGCQVVADT
TQLWETADIILKVRAPQMHPTLNRHEAELLNEGKTLISFIWPAQNEDLLKQLAEQKANVLAMDAIPRISRAQKMDALSSM
ANIAGYRAVVESANNFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAVVRSFDTRPVVKEQVQSMGAEFLE
LEFEEDGTGAGGYAKTMSPEFIAAEMELFAQQAEEVDIIITTALIPGKKAPLLITKDMVESMKEGSVVVDMAAEQGGNCE
VTRPNEVYQHHGVTIVGLTDLPSRMANQSSQLYGNNLCHLLKDMGGAEDYKIDFEDEVVRGATVLQEGKITWPPPQVSQP
SPPKPAAEAKQPAMPAEPEVKEKSVIGQWLPLIAVGAALFGIGLTAPPSFLTHFTVFVLACFVGWQVIWNVAPALHTPLM
SVTNAISGIIILGGMLQISGPLGSPTTILGAIAILVGTINIAGGFLVTQRMLQMFRR

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:
GO:0008750: NAD(P) transhydrogenase, alpha subunit
GO:0015992: NAD(P) transhydrogenase, alpha subunit
GO:0016491: NAD(P) transhydrogenase, alpha subunit
GO:0055114: NAD(P) transhydrogenase, alpha subunit

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=544, Percent_Identity=52.5735294117647, Blast_Score=540, Evalue=1e-153,
Organism=Homo sapiens, GI122939153, Length=544, Percent_Identity=52.5735294117647, Blast_Score=540, Evalue=1e-153,
Organism=Escherichia coli, GI1787887, Length=526, Percent_Identity=59.5057034220532, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17550456, Length=523, Percent_Identity=53.5372848948375, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 57399; Mature: 57268

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVADKKLANADNPQDPKTGLKIGIPKEVFPGERRVAATPDTAKTLQKMGFEVLVESKAG
CCCCCCHHCCCCCCCCCCCCCEECCCHHHCCCCCEEECCCHHHHHHHHCCCEEEEECCCC
AGATFSDQVYQQAGCQVVADTTQLWETADIILKVRAPQMHPTLNRHEAELLNEGKTLISF
CCCCHHHHHHHHHCCEEEECHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEE
IWPAQNEDLLKQLAEQKANVLAMDAIPRISRAQKMDALSSMANIAGYRAVVESANNFGRF
ECCCCCHHHHHHHHHHHCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCE
FTGQITAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAVVRSFDTRPVVKEQVQSMGAEFL
EEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHE
ELEFEEDGTGAGGYAKTMSPEFIAAEMELFAQQAEEVDIIITTALIPGKKAPLLITKDMV
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEHHHH
ESMKEGSVVVDMAAEQGGNCEVTRPNEVYQHHGVTIVGLTDLPSRMANQSSQLYGNNLCH
HHHHCCCEEEEEECCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHHH
LLKDMGGAEDYKIDFEDEVVRGATVLQEGKITWPPPQVSQPSPPKPAAEAKQPAMPAEPE
HHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCC
VKEKSVIGQWLPLIAVGAALFGIGLTAPPSFLTHFTVFVLACFVGWQVIWNVAPALHTPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSVTNAISGIIILGGMLQISGPLGSPTTILGAIAILVGTINIAGGFLVTQRMLQMFRR
HHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVADKKLANADNPQDPKTGLKIGIPKEVFPGERRVAATPDTAKTLQKMGFEVLVESKAG
CCCCCHHCCCCCCCCCCCCCEECCCHHHCCCCCEEECCCHHHHHHHHCCCEEEEECCCC
AGATFSDQVYQQAGCQVVADTTQLWETADIILKVRAPQMHPTLNRHEAELLNEGKTLISF
CCCCHHHHHHHHHCCEEEECHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEE
IWPAQNEDLLKQLAEQKANVLAMDAIPRISRAQKMDALSSMANIAGYRAVVESANNFGRF
ECCCCCHHHHHHHHHHHCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCE
FTGQITAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAVVRSFDTRPVVKEQVQSMGAEFL
EEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHE
ELEFEEDGTGAGGYAKTMSPEFIAAEMELFAQQAEEVDIIITTALIPGKKAPLLITKDMV
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEHHHH
ESMKEGSVVVDMAAEQGGNCEVTRPNEVYQHHGVTIVGLTDLPSRMANQSSQLYGNNLCH
HHHHCCCEEEEEECCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHHH
LLKDMGGAEDYKIDFEDEVVRGATVLQEGKITWPPPQVSQPSPPKPAAEAKQPAMPAEPE
HHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCC
VKEKSVIGQWLPLIAVGAALFGIGLTAPPSFLTHFTVFVLACFVGWQVIWNVAPALHTPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSVTNAISGIIILGGMLQISGPLGSPTTILGAIAILVGTINIAGGFLVTQRMLQMFRR
HHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]