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The map label for this gene is rlmN

Identifier: 158337109

GI number: 158337109

Start: 4032496

End: 4033551

Strand: Reverse

Name: rlmN

Synonym: AM1_3982

Alternate gene names: 158337109

Gene position: 4033551-4032496 (Counterclockwise)

Preceding gene: 158337111

Following gene: 158337105

Centisome position: 62.02

GC content: 47.92

Gene sequence:

>1056_bases
ATGCTTGATCAACTTTCTGTAGAGTCTATTCCACCCCTTCTGGGGGCGTCTAAGGCTCAGTTGACCCATTGGGCTCAGAC
TTATCAGCAGCCAGCCTATCGGGGACAACAAGTACACCAATGGATTTACCAGAAAGGTGTGCATTCCCTCAGCGACATCA
CGGTTCTCCCAAAACAATGGCGGACTGAAATTGCAGATATTCCCGTGGGCCGTTCCCAGATTCACCATCGTTCTGCCGCT
CAAGATGGGACCGTCAAGTATTTACTCAAACTTGCAGATGGGCAAATCATTGAAACTGTTGGCATCCCAACCCAAAAACG
TCTAACCGTTTGCGTTTCTTCCCAGGTTGGCTGTCCTATGGGCTGCGACTTTTGTGCCACTGGCAAAGGCGAATATCAAC
GCAATCTTGCCTGTCATGAAATCGTTGACCAAGTCTTAACGGTCCAAGAAGATTTTCAACAGCGAGTGGGCAATGTTGTT
TTTATGGGCATGGGGGAACCCCTGTTGAATCTCGAACAAGTGCTGGCAGCCGTACGATCCCTCAACCAAGATGTGGGGAT
TGGTCAACGATCCCTGACGGTTTCCACCGTCGGCATTCCCAAGCAAATCCTCAAACTTGCCCAGCATCAACTTCAGATTA
CTTTAGCAGTCAGCTTGCATGCGTCTAATCAGAGAATTCGCACACAGCTCGTTCCAAGCGCCAAACATTATCCCTTGGAG
AAACTTCTAAAAGATTGTCGGGCTTACGTTACCCAAACTGGACGCCGTGTCACCTTTGAGTACATTGTGTTGTCCGGTGT
CAACGATCAAACAGAACATGCTCTAGAGTTAGCCCATCATTTGCGAGGCTTCCAAAGTCATGTGAACTTAATTCCTTACA
ACCCGATCTCTGAAGTTGACTATCAACGCCCAACCCAAAAGCAACTCCAACAGTTTCTGAACAGCTTACAAAGCCAACAT
ATTACGGCTAGTATTCGGCGATCTCGTGGTTTAGACAAAGATGCTGCCTGTGGTCAATTAAGGGCAACGCAAATGATAGA
TCCAGCCTCAAGCTAA

Upstream 100 bases:

>100_bases
CACATCAAGCTTTATAGTATATTTCCATTCTTCCAACTCCTGCTAACGCTACCTCAACTGGGCGTTTCTCACTGTTTCTC
TCAGGCCATCCATAACCATC

Downstream 100 bases:

>100_bases
CAGGCCAAACAATTCACTTTGCTGAAGTTCTGGGTATCCATAGCAATTTAAAGACAATAAATAAGGCAGTGTGTTCTCAC
ACTGCCTTATTTCAGCCTAA

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLDQLSVESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQWRTEIADIPVGRSQIHHRSAA
QDGTVKYLLKLADGQIIETVGIPTQKRLTVCVSSQVGCPMGCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVV
FMGMGEPLLNLEQVLAAVRSLNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLE
KLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAHHLRGFQSHVNLIPYNPISEVDYQRPTQKQLQQFLNSLQSQH
ITASIRRSRGLDKDAACGQLRATQMIDPASS

Sequences:

>Translated_351_residues
MLDQLSVESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQWRTEIADIPVGRSQIHHRSAA
QDGTVKYLLKLADGQIIETVGIPTQKRLTVCVSSQVGCPMGCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVV
FMGMGEPLLNLEQVLAAVRSLNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLE
KLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAHHLRGFQSHVNLIPYNPISEVDYQRPTQKQLQQFLNSLQSQH
ITASIRRSRGLDKDAACGQLRATQMIDPASS
>Mature_351_residues
MLDQLSVESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQWRTEIADIPVGRSQIHHRSAA
QDGTVKYLLKLADGQIIETVGIPTQKRLTVCVSSQVGCPMGCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVV
FMGMGEPLLNLEQVLAAVRSLNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLE
KLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAHHLRGFQSHVNLIPYNPISEVDYQRPTQKQLQQFLNSLQSQH
ITASIRRSRGLDKDAACGQLRATQMIDPASS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=348, Percent_Identity=37.0689655172414, Blast_Score=227, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ACAM1 (B0C9F4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518284.1
- ProteinModelPortal:   B0C9F4
- SMR:   B0C9F4
- GeneID:   5682786
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3982
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK2475842
- BioCyc:   AMAR329726:AM1_3982-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39175; Mature: 39175

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQLSVESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQW
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHHEECCHHH
RTEIADIPVGRSQIHHRSAAQDGTVKYLLKLADGQIIETVGIPTQKRLTVCVSSQVGCPM
HHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCHHEEEEECCCCCCCC
GCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVVFMGMGEPLLNLEQVLAAVRS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
LNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLE
CCHHCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHCCCCCCCCHH
KLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAHHLRGFQSHVNLIPYNPISEVD
HHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YQRPTQKQLQQFLNSLQSQHITASIRRSRGLDKDAACGQLRATQMIDPASS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCCC
>Mature Secondary Structure
MLDQLSVESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQW
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHHEECCHHH
RTEIADIPVGRSQIHHRSAAQDGTVKYLLKLADGQIIETVGIPTQKRLTVCVSSQVGCPM
HHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCHHEEEEECCCCCCCC
GCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVVFMGMGEPLLNLEQVLAAVRS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
LNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLE
CCHHCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHCCCCCCCCHH
KLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAHHLRGFQSHVNLIPYNPISEVD
HHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YQRPTQKQLQQFLNSLQSQHITASIRRSRGLDKDAACGQLRATQMIDPASS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA