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The map label for this gene is ispD

Identifier: 158337111

GI number: 158337111

Start: 4034376

End: 4035089

Strand: Reverse

Name: ispD

Synonym: AM1_3984

Alternate gene names: 158337111

Gene position: 4035089-4034376 (Counterclockwise)

Preceding gene: 158337112

Following gene: 158337109

Centisome position: 62.04

GC content: 50.0

Gene sequence:

>714_bases
ATGCACTTATTAATTCCGGCAGCAGGGATGGGAAGACGCATGGGCAGCGATCGCAATAAGTTACGATTAATGTTGCACGG
CAAGCCCCTGTTAGCCTGGACGCTGCTGGCAGCCGAATCTTCCATCTCCATTTCCTGGATTGGAATTATCGGTCAATTAG
AGGATGAACCAACCTGGGAAACACTTTTAAACACCCTCAGCCTCAATAAACCTGTCGTCTTTATCGAAGGGGGACAAACT
CGCCAAGAATCCGTTTACAACGGTCTCCAAGCACTCCCCCCTGAAGCAGAACAAGTACTCATCCACGATGGTGCCCGTTG
TCTGGCCACACCAGAGCTTCTAGACCGTTGTGCTCACGCCCTCCATAAATATCAAGGTTTTGTCGCCGCTGTGCCGGTCA
AGGACACCATTAAGGTGGTCGATACCACCCATCAAATTCAAGGCACGCCTGACCGATCCCAGTTATGGGCAGCCCAGACT
CCTCAAGGGTTCCAAGTAGTACCTCTTAAGAAGTGCCACGAGCAAGGTCGACAGCAAGGCTGGGAAGTCACGGACGATGC
AGCTTTGTTTGAGAAATTAGACCTTCCAGTTCATATCGTTGTAGGGGAAGAAACGAACTTAAAAGTGACCACGCCGGCTG
ATCTTGCGATCGCAGAACTGATTCTTGAACAACGTCTACAAAAAAACTCCCAAACTGACGGATTTGGGAGTTGA

Upstream 100 bases:

>100_bases
TCAACGCTTGTAATCAATCCGCTCTGGAACTCAATAAGTCTTCTAACTTGCCGTCTCAGGTCAGTATAGGATAAGGAATG
GCAGCAGTTTATGGTTATCA

Downstream 100 bases:

>100_bases
TTCGTAGATTATTACCACAGTAGAAAAACTGGGAAGGTTGCCGCGATTTATCCTTTCCGTGAGTAGTATTCCACCACAAG
TAGTTCGTTAATTTCTAGCG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MHLLIPAAGMGRRMGSDRNKLRLMLHGKPLLAWTLLAAESSISISWIGIIGQLEDEPTWETLLNTLSLNKPVVFIEGGQT
RQESVYNGLQALPPEAEQVLIHDGARCLATPELLDRCAHALHKYQGFVAAVPVKDTIKVVDTTHQIQGTPDRSQLWAAQT
PQGFQVVPLKKCHEQGRQQGWEVTDDAALFEKLDLPVHIVVGEETNLKVTTPADLAIAELILEQRLQKNSQTDGFGS

Sequences:

>Translated_237_residues
MHLLIPAAGMGRRMGSDRNKLRLMLHGKPLLAWTLLAAESSISISWIGIIGQLEDEPTWETLLNTLSLNKPVVFIEGGQT
RQESVYNGLQALPPEAEQVLIHDGARCLATPELLDRCAHALHKYQGFVAAVPVKDTIKVVDTTHQIQGTPDRSQLWAAQT
PQGFQVVPLKKCHEQGRQQGWEVTDDAALFEKLDLPVHIVVGEETNLKVTTPADLAIAELILEQRLQKNSQTDGFGS
>Mature_237_residues
MHLLIPAAGMGRRMGSDRNKLRLMLHGKPLLAWTLLAAESSISISWIGIIGQLEDEPTWETLLNTLSLNKPVVFIEGGQT
RQESVYNGLQALPPEAEQVLIHDGARCLATPELLDRCAHALHKYQGFVAAVPVKDTIKVVDTTHQIQGTPDRSQLWAAQT
PQGFQVVPLKKCHEQGRQQGWEVTDDAALFEKLDLPVHIVVGEETNLKVTTPADLAIAELILEQRLQKNSQTDGFGS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:
GO:0003824: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
GO:0008299: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
GO:0016740: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
GO:0016779: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
GO:0050518: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=32.1739130434783, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_ACAM1 (B0C9F6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518286.1
- ProteinModelPortal:   B0C9F6
- SMR:   B0C9F6
- GeneID:   5682788
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3984
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   AMAR329726:AM1_3984-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26117; Mature: 26117

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLIPAAGMGRRMGSDRNKLRLMLHGKPLLAWTLLAAESSISISWIGIIGQLEDEPTWE
CEEEECCCCCCHHCCCCCCEEEEEECCCCHHHHHEECCCCCCEEEEEEEEECCCCCCHHH
TLLNTLSLNKPVVFIEGGQTRQESVYNGLQALPPEAEQVLIHDGARCLATPELLDRCAHA
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHEEEEECCCCEEECHHHHHHHHHH
LHKYQGFVAAVPVKDTIKVVDTTHQIQGTPDRSQLWAAQTPQGFQVVPLKKCHEQGRQQG
HHHHCCEEEEECCCHHEEEEECHHCCCCCCCHHHEEECCCCCCCEEECHHHHHHHHHHCC
WEVTDDAALFEKLDLPVHIVVGEETNLKVTTPADLAIAELILEQRLQKNSQTDGFGS
CCCCCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHLLIPAAGMGRRMGSDRNKLRLMLHGKPLLAWTLLAAESSISISWIGIIGQLEDEPTWE
CEEEECCCCCCHHCCCCCCEEEEEECCCCHHHHHEECCCCCCEEEEEEEEECCCCCCHHH
TLLNTLSLNKPVVFIEGGQTRQESVYNGLQALPPEAEQVLIHDGARCLATPELLDRCAHA
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHEEEEECCCCEEECHHHHHHHHHH
LHKYQGFVAAVPVKDTIKVVDTTHQIQGTPDRSQLWAAQTPQGFQVVPLKKCHEQGRQQG
HHHHCCEEEEECCCHHEEEEECHHCCCCCCCHHHEEECCCCCCCEEECHHHHHHHHHHCC
WEVTDDAALFEKLDLPVHIVVGEETNLKVTTPADLAIAELILEQRLQKNSQTDGFGS
CCCCCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA