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The map label for this gene is eno

Identifier: 158337105

GI number: 158337105

Start: 4028222

End: 4029502

Strand: Reverse

Name: eno

Synonym: AM1_3978

Alternate gene names: 158337105

Gene position: 4029502-4028222 (Counterclockwise)

Preceding gene: 158337109

Following gene: 158337102

Centisome position: 61.96

GC content: 49.88

Gene sequence:

>1281_bases
ATGCACGAAACCGAAATCGCTGCCATTGAGGCGCGCGAAATTCTAGACTCACGCGGTCGCCCTACTGTGGAAGCAGAGGT
CATATTAAGTTGTGGGGCTTTTGGATTGGCCCAAGTTCCCAGTGGTGCTTCTACAGGTAGTTTTGAAGCCCATGAGCTGA
GAGATGGGGATGCTCAACGCTATGGTGGCAAAGGGGTGCTTACTGCCGTTACTAATATTAAGGATCACATTCAGCCTGAG
CTGGTCGGGTTAAATGCCCTCGATCAGGCTGATATCGATCAGACCATGATTGATCTGGATGGATCGACGAACAAATCTAA
ACTGGGTGCAAATGCCATCTTGGCTGTCTCCCTTGCCACGGCTAAAGCCGCTTCTGAAGTACTGAATTTAGAGCTATATC
GTTATCTAGGAGGTCCTTTAGCAAACCTCTTGCCGGTGCCCTTAATGAACGTCATCAACGGTGGAGCCCATGCCAACAAT
AACGTTGATATTCAAGAGTTTATGATTGTCCCCCACGGTGCAGATTCCTTTAAGGAAGCCCTGCGCTGGGGAGCAGAAGT
CTTTGCATCGTTGAGTCAAGTATTGGCAGCCCAAAATCTGTTAACAGGCGTGGGGGATGAGGGGGGCTATGCCCCCAATC
TGGCGTCTAACCAAGCCGCGTTGGAATTGTTAGTGGAAGCGATTGAGAAGGCTGGCTATCAACCTGGCGCTCAAATTAGC
TTGGCCTTAGATGTCGCAGCCAGCGAATTCTATAAAGACGGGCAGTATACCTACGACGGTGCCGCCCATTCCCCCAAGGA
GCTAATCGACTATTTAGATGGCTTAGCCAGTAAATATCCGATTGTCTCCATTGAAGATCCTCTACATGAAGAAGATTGGG
AGAGTTGGGGGAATTTGACAGCCCAGTTAGGGGATCGAGTACAGCTCGTCGGGGATGACCTGTTCGTCACGAACCCTATC
CGGTTACAAAAAGGAATTGAGCAGCAATCAGCCAACTCTATTTTGATTAAGCTAAATCAAATCGGTACCTTGACCGAAAC
CCTAGAAACAATTGATCTAGCCAAACGCAATTCCTTTACGTCCATTATTAGTCATCGATCCGGTGAGACGGAAGATACCA
CGATTGCGGATCTCGCGGTTGCGACCCGAGCCGGTCAGATCAAAACGGGATCTCTTTGCCGTAGCGAACGCGTGGCAAAA
TACAATCGCCTGCTACGCATCGAGGATGAACTCGGCAGTCAAGCGTCCTATGCTGGCGCAGTAGGCTTAGGGCCAGGGTA
A

Upstream 100 bases:

>100_bases
CGTTGCATCAACGCCGCTGATACAGTCAGATGCATTTTATAGCCTTGACGAGCAGGTCAAGCACACCAGATTATTTTGTA
GGTTGTTGGAAACATAACGC

Downstream 100 bases:

>100_bases
GACTCTTCTTCAGAGTGGCGATTTTAACCACTCCGAAGAAGAGTCTTCTGTTAAAGCGATGTGAACGCAATAGATGGGCG
TCTTTGCAGTTGCTGCTCTA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MHETEIAAIEAREILDSRGRPTVEAEVILSCGAFGLAQVPSGASTGSFEAHELRDGDAQRYGGKGVLTAVTNIKDHIQPE
LVGLNALDQADIDQTMIDLDGSTNKSKLGANAILAVSLATAKAASEVLNLELYRYLGGPLANLLPVPLMNVINGGAHANN
NVDIQEFMIVPHGADSFKEALRWGAEVFASLSQVLAAQNLLTGVGDEGGYAPNLASNQAALELLVEAIEKAGYQPGAQIS
LALDVAASEFYKDGQYTYDGAAHSPKELIDYLDGLASKYPIVSIEDPLHEEDWESWGNLTAQLGDRVQLVGDDLFVTNPI
RLQKGIEQQSANSILIKLNQIGTLTETLETIDLAKRNSFTSIISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAK
YNRLLRIEDELGSQASYAGAVGLGPG

Sequences:

>Translated_426_residues
MHETEIAAIEAREILDSRGRPTVEAEVILSCGAFGLAQVPSGASTGSFEAHELRDGDAQRYGGKGVLTAVTNIKDHIQPE
LVGLNALDQADIDQTMIDLDGSTNKSKLGANAILAVSLATAKAASEVLNLELYRYLGGPLANLLPVPLMNVINGGAHANN
NVDIQEFMIVPHGADSFKEALRWGAEVFASLSQVLAAQNLLTGVGDEGGYAPNLASNQAALELLVEAIEKAGYQPGAQIS
LALDVAASEFYKDGQYTYDGAAHSPKELIDYLDGLASKYPIVSIEDPLHEEDWESWGNLTAQLGDRVQLVGDDLFVTNPI
RLQKGIEQQSANSILIKLNQIGTLTETLETIDLAKRNSFTSIISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAK
YNRLLRIEDELGSQASYAGAVGLGPG
>Mature_426_residues
MHETEIAAIEAREILDSRGRPTVEAEVILSCGAFGLAQVPSGASTGSFEAHELRDGDAQRYGGKGVLTAVTNIKDHIQPE
LVGLNALDQADIDQTMIDLDGSTNKSKLGANAILAVSLATAKAASEVLNLELYRYLGGPLANLLPVPLMNVINGGAHANN
NVDIQEFMIVPHGADSFKEALRWGAEVFASLSQVLAAQNLLTGVGDEGGYAPNLASNQAALELLVEAIEKAGYQPGAQIS
LALDVAASEFYKDGQYTYDGAAHSPKELIDYLDGLASKYPIVSIEDPLHEEDWESWGNLTAQLGDRVQLVGDDLFVTNPI
RLQKGIEQQSANSILIKLNQIGTLTETLETIDLAKRNSFTSIISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAK
YNRLLRIEDELGSQASYAGAVGLGPG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:
GO:0000015: Enolase
GO:0000287: Enolase
GO:0004634: Enolase
GO:0005576: Enolase
GO:0005737: Enolase
GO:0006096: Enolase
GO:0009986: Enolase
GO:0016829: Enolase
GO:0046872: Enolase

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=52.1028037383178, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=51.288056206089, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=51.288056206089, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=46.5882352941176, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=28.023598820059, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=28.023598820059, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=28.023598820059, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=32.8, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=32.8, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=32.8, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=59.5744680851064, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=52.4475524475524, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=52.4475524475524, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=51.5789473684211, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.2678983833718, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.2678983833718, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.0369515011547, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321693, Length=435, Percent_Identity=48.5057471264368, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=46.9107551487414, Blast_Score=349, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=51.9630484988453, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=51.9630484988453, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=51.9630484988453, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=51.9630484988453, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=52.2144522144522, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=52.2144522144522, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ACAM1 (B0C9F0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518280.1
- ProteinModelPortal:   B0C9F0
- SMR:   B0C9F0
- GeneID:   5682782
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3978
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   AMAR329726:AM1_3978-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45337; Mature: 45337

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 337-337 BINDING 157-157 BINDING 166-166 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHETEIAAIEAREILDSRGRPTVEAEVILSCGAFGLAQVPSGASTGSFEAHELRDGDAQR
CCCCHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCHHHCCCCCCCCCCCHHHCCCCCHHH
YGGKGVLTAVTNIKDHIQPELVGLNALDQADIDQTMIDLDGSTNKSKLGANAILAVSLAT
CCCCEEEHHHHHHHHHCCCCEECCCCCCCCCCCHHEEECCCCCCCHHCCCCEEEEEEHHH
AKAASEVLNLELYRYLGGPLANLLPVPLMNVINGGAHANNNVDIQEFMIVPHGADSFKEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHH
LRWGAEVFASLSQVLAAQNLLTGVGDEGGYAPNLASNQAALELLVEAIEKAGYQPGAQIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
LALDVAASEFYKDGQYTYDGAAHSPKELIDYLDGLASKYPIVSIEDPLHEEDWESWGNLT
EEEEHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCHH
AQLGDRVQLVGDDLFVTNPIRLQKGIEQQSANSILIKLNQIGTLTETLETIDLAKRNSFT
HHHCCEEEEECCCEEECCCHHHHHCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCHHH
SIISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAKYNRLLRIEDELGSQASYAGA
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHCCCCCCCCCC
VGLGPG
CCCCCC
>Mature Secondary Structure
MHETEIAAIEAREILDSRGRPTVEAEVILSCGAFGLAQVPSGASTGSFEAHELRDGDAQR
CCCCHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCHHHCCCCCCCCCCCHHHCCCCCHHH
YGGKGVLTAVTNIKDHIQPELVGLNALDQADIDQTMIDLDGSTNKSKLGANAILAVSLAT
CCCCEEEHHHHHHHHHCCCCEECCCCCCCCCCCHHEEECCCCCCCHHCCCCEEEEEEHHH
AKAASEVLNLELYRYLGGPLANLLPVPLMNVINGGAHANNNVDIQEFMIVPHGADSFKEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHH
LRWGAEVFASLSQVLAAQNLLTGVGDEGGYAPNLASNQAALELLVEAIEKAGYQPGAQIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
LALDVAASEFYKDGQYTYDGAAHSPKELIDYLDGLASKYPIVSIEDPLHEEDWESWGNLT
EEEEHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCHH
AQLGDRVQLVGDDLFVTNPIRLQKGIEQQSANSILIKLNQIGTLTETLETIDLAKRNSFT
HHHCCEEEEECCCEEECCCHHHHHCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCHHH
SIISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAKYNRLLRIEDELGSQASYAGA
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHCCCCCCCCCC
VGLGPG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA