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The map label for this gene is pfkA [H]

Identifier: 158337074

GI number: 158337074

Start: 3997173

End: 3998255

Strand: Reverse

Name: pfkA [H]

Synonym: AM1_3947

Alternate gene names: 158337074

Gene position: 3998255-3997173 (Counterclockwise)

Preceding gene: 158337076

Following gene: 158337072

Centisome position: 61.48

GC content: 52.26

Gene sequence:

>1083_bases
ATGGGTGATACAAAACGGATCGGTATTCTGACGAGCGGCGGTGATTGTGCAGGGCTTAACCCCACGATTCGCGCAGTCGT
GCTTCGAGCAGTTGAACATTATGGTTGGGAAGTGATCGGGATTCAACAAGCCACCCATGGTTTATTGAAAGATCCGCCTG
CAGCCATGGCGCTGCAACCTCAACAGATGAGTCCTTTACTCACCGCAGGAGGGACGATGTTAGGAACAACCAACAAGGGG
AATCCCTTTGCCTTTCCGATGCCAGATGGCACGCTGATCGATCGCTCAGAAGATATGATTGACGGCTATCGCAAGCTGGG
CTTAGACGCTCTGATCGGGATTGGCGGCGATGGTAGCATCGCCATTCTCCGTAAGCTAGCCCAACAGGGTAATTGGAACT
TGGTGGGCATTCCCAAAACCATCGACAATGATGTGGGAGTAACGGAACGATCGGTCGGATTTGATACGGCGGTCAATATT
GCCACAGAAGCCCTAGACCGCTTGCAATTTACTGCAGCCAGCCATAGCCGTGTCATTATCCTGGAGGTGATGGGTCGGGA
TGCGGGTCATATTGCGATCAGTGCAGGTATTGCCGGAGGGGCAGATATCATCTTGATTCCTGAAATTCCCTACACCATTG
ATAATGTCTGCGCCATAATCAAAGAACGCCAGAGTAAGGGCAAAAACTATTCTCTGATAATCGTGGCTGAGGCGGTGCGG
ACTGAGGGCGGTGATCCCTTAACCCATACAGATGGATTAGGCGAATGCCGTCTGGGGGGAATCGGCCAATATTTATCTGA
GCAAATTTGCAGTCGTCATGTGGCCGAAACCCGGGTGACAGTTTTAGGGCATGTTCAGCGCGGCGGCATACCGTCCCCTT
CCGATCGACTAATTGGGGCAGCGTTTGGCGTTGCGGCCGTCGATTTGATTGCTGAACGTCAGTTTGATCAAATGGTGACT
TGGCAAAATCGCCAGGTGATGAGTGTTCCGATTGCTGATGCGATCGCACATTACAGAGCCGTCGACCCCACCGGAACGCT
GGTCAAAACCGCCAGAGGTTTAGGGATTTACCTAGGAGACTAG

Upstream 100 bases:

>100_bases
CAAAGATAAATTCAACCTGGATTTCCCTATGATCCTGCCATCTATGGTTTAGGTTACATCTATCTTTTAATGCCAATCCT
CTGGGTCATAGTGAGTAAAT

Downstream 100 bases:

>100_bases
AGGGCTACAACCCTTTCACACCTGATTTCACACCTAAAATTTGGCCACCCCCATTTTTAGGTGTGAAATCTCTGGGTGGC
CCTAGGCCGCTGCTGCCAAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MGDTKRIGILTSGGDCAGLNPTIRAVVLRAVEHYGWEVIGIQQATHGLLKDPPAAMALQPQQMSPLLTAGGTMLGTTNKG
NPFAFPMPDGTLIDRSEDMIDGYRKLGLDALIGIGGDGSIAILRKLAQQGNWNLVGIPKTIDNDVGVTERSVGFDTAVNI
ATEALDRLQFTAASHSRVIILEVMGRDAGHIAISAGIAGGADIILIPEIPYTIDNVCAIIKERQSKGKNYSLIIVAEAVR
TEGGDPLTHTDGLGECRLGGIGQYLSEQICSRHVAETRVTVLGHVQRGGIPSPSDRLIGAAFGVAAVDLIAERQFDQMVT
WQNRQVMSVPIADAIAHYRAVDPTGTLVKTARGLGIYLGD

Sequences:

>Translated_360_residues
MGDTKRIGILTSGGDCAGLNPTIRAVVLRAVEHYGWEVIGIQQATHGLLKDPPAAMALQPQQMSPLLTAGGTMLGTTNKG
NPFAFPMPDGTLIDRSEDMIDGYRKLGLDALIGIGGDGSIAILRKLAQQGNWNLVGIPKTIDNDVGVTERSVGFDTAVNI
ATEALDRLQFTAASHSRVIILEVMGRDAGHIAISAGIAGGADIILIPEIPYTIDNVCAIIKERQSKGKNYSLIIVAEAVR
TEGGDPLTHTDGLGECRLGGIGQYLSEQICSRHVAETRVTVLGHVQRGGIPSPSDRLIGAAFGVAAVDLIAERQFDQMVT
WQNRQVMSVPIADAIAHYRAVDPTGTLVKTARGLGIYLGD
>Mature_359_residues
GDTKRIGILTSGGDCAGLNPTIRAVVLRAVEHYGWEVIGIQQATHGLLKDPPAAMALQPQQMSPLLTAGGTMLGTTNKGN
PFAFPMPDGTLIDRSEDMIDGYRKLGLDALIGIGGDGSIAILRKLAQQGNWNLVGIPKTIDNDVGVTERSVGFDTAVNIA
TEALDRLQFTAASHSRVIILEVMGRDAGHIAISAGIAGGADIILIPEIPYTIDNVCAIIKERQSKGKNYSLIIVAEAVRT
EGGDPLTHTDGLGECRLGGIGQYLSEQICSRHVAETRVTVLGHVQRGGIPSPSDRLIGAAFGVAAVDLIAERQFDQMVTW
QNRQVMSVPIADAIAHYRAVDPTGTLVKTARGLGIYLGD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=31.6804407713499, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=31.6804407713499, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=31.6804407713499, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=31.6804407713499, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI48762920, Length=368, Percent_Identity=30.1630434782609, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI11321601, Length=362, Percent_Identity=30.3867403314917, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI1790350, Length=339, Percent_Identity=34.8082595870207, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17558788, Length=328, Percent_Identity=32.0121951219512, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25147584, Length=333, Percent_Identity=29.1291291291291, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI72003601, Length=165, Percent_Identity=35.1515151515151, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323861, Length=367, Percent_Identity=28.8828337874659, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=32.0699708454811, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI28573326, Length=365, Percent_Identity=31.2328767123288, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24652337, Length=365, Percent_Identity=30.6849315068493, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17647809, Length=365, Percent_Identity=30.6849315068493, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38088; Mature: 37957

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTKRIGILTSGGDCAGLNPTIRAVVLRAVEHYGWEVIGIQQATHGLLKDPPAAMALQP
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCHHCCCCHHEEECH
QQMSPLLTAGGTMLGTTNKGNPFAFPMPDGTLIDRSEDMIDGYRKLGLDALIGIGGDGSI
HHCCCHHHCCCEEEECCCCCCEEEEECCCCCEECCCHHHHHHHHHCCCCEEEEECCCCHH
AILRKLAQQGNWNLVGIPKTIDNDVGVTERSVGFDTAVNIATEALDRLQFTAASHSRVII
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEE
LEVMGRDAGHIAISAGIAGGADIILIPEIPYTIDNVCAIIKERQSKGKNYSLIIVAEAVR
EEEECCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEHH
TEGGDPLTHTDGLGECRLGGIGQYLSEQICSRHVAETRVTVLGHVQRGGIPSPSDRLIGA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHHH
AFGVAAVDLIAERQFDQMVTWQNRQVMSVPIADAIAHYRAVDPTGTLVKTARGLGIYLGD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHCCCCEEEECC
>Mature Secondary Structure 
GDTKRIGILTSGGDCAGLNPTIRAVVLRAVEHYGWEVIGIQQATHGLLKDPPAAMALQP
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCHHCCCCHHEEECH
QQMSPLLTAGGTMLGTTNKGNPFAFPMPDGTLIDRSEDMIDGYRKLGLDALIGIGGDGSI
HHCCCHHHCCCEEEECCCCCCEEEEECCCCCEECCCHHHHHHHHHCCCCEEEEECCCCHH
AILRKLAQQGNWNLVGIPKTIDNDVGVTERSVGFDTAVNIATEALDRLQFTAASHSRVII
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEE
LEVMGRDAGHIAISAGIAGGADIILIPEIPYTIDNVCAIIKERQSKGKNYSLIIVAEAVR
EEEECCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEHH
TEGGDPLTHTDGLGECRLGGIGQYLSEQICSRHVAETRVTVLGHVQRGGIPSPSDRLIGA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHHH
AFGVAAVDLIAERQFDQMVTWQNRQVMSVPIADAIAHYRAVDPTGTLVKTARGLGIYLGD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]