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The map label for this gene is pdhC [H]

Identifier: 158336703

GI number: 158336703

Start: 3620224

End: 3621564

Strand: Direct

Name: pdhC [H]

Synonym: AM1_3571

Alternate gene names: 158336703

Gene position: 3620224-3621564 (Clockwise)

Preceding gene: 158336701

Following gene: 158336706

Centisome position: 55.66

GC content: 56.0

Gene sequence:

>1341_bases
ATGATTCATGAAGTGTTTATGCCTGCCCTCAGCTCCACGATGGAGGAGGGAAAAATTGTCTCTTGGTCTAAGGAGCCAGG
TGACAAGGTCGAAAAGGGTGAAACGGTTCTGGTGGTTGAATCCGACAAGGCAGATATGGATGTGGAGTCCTTCCATGAGG
GATATCTAGCAGCCATCGCGGTGCCTGCGGGTGGGGTGGCCAAGGTCGGTGCGGCCATTGGCTATGTTGCCGAAACGGAA
GCTGAAATTGCTGAAGCACAAAAGAAAGCATCTGCCGCTGAGTCTGCGGCCCCTGCACCTGCAGCTCCGGCCCCTGCACC
TGCAGCTCCAGCCCCTGCTGCAGTAGCTCCGGCTCCTCCGGCTGCGGCCCCGGCCCCTGTTGCTACTATTCCAGTAGCCC
CAGCAGCTACCTTAAATGGAGGCAGTGCACCGGCTGCTCCATCTAACGGTCGAGTGGTGGTTTCACCTCGGGCGCGGAAG
CTTGCTAAGCAATTCAAAGTCGATCTCAATACCCTAACGGGCAGTGGTCCCCATGGTCGAATCGTGGCTGCAGATATAGA
AGCAGCCTCTGGCCAAACGTCGACCACTGCTACGGCTCCAGCCGCCAGCAGTGCTGCGCCTCAGCCATCCCTACCTGCCT
CCGCTCCCTTACCCGCTGGAGCAGCAGCCGGTGAAGTAGTTCCCTTCAATACCTTGCAGCAGGCTGTGGTGAACAACATG
GTGGCTAGTCTAGCTGTGCCCACCTTCCATGTGGAATATTCCATTGTTACAGATGCCCTGGACCAGCTGTATAAGCAAGT
CAAGACCAAAGGTGTCACTATGACGGCCCTCTTGGCCAAAGCTGTGGCGGTTACCCTGCGCCAGCATCCCCTGGTCAATG
CCAGCTGTGCCCCACAAGGAATCCAGTACAGCAGCGCCATCAATATTGCTGTGGCGGTTGCCATGCCTGGGGGCGGTTTG
ATTACGCCAGTTCTGCAACAGGCGGATCAGATGGATTTATATTCCCTCTCTCGCACTTGGAGAGATTTAGTTGCTCGCGC
CCGTAGCAAACAGCTGCAGCCGGATGAATACAGTACCGGCACCTTTACCCTTTCCAATCTGGGAATGTTTGGGGTCAACA
GCTTTGATGCCATTCTGCCCCCAGGGCAAGGGTCCATTCTGGCGATTGGTGGGTCTAAGCCCCAAGTCGTGGCGGATGAT
CAGGGCATGATGGGGGTGAAGCGACTGATGAATGTCAACATTACCTGCGATCACCGGGTGATCTATGGTGCCGATGCCGC
TGCCTTTTTGAAGGATTTAGCGGAGTTGATTGAAACCAATCCCCAATCCTTGACCCTGTAA

Upstream 100 bases:

>100_bases
TTTCTCGGTAAAGGAGATAGTGTGGATAGCCCTGAACAGGATAAAATTCAAACTGGATAGGCTTTGGTTTATCTTGCTAT
TCGTTTCGGAAAACTAACCC

Downstream 100 bases:

>100_bases
TCCATAGACGGGAGCTTCATCATGAAGGCTTCTCCTTTTGTGAAGCTTCTGATTACAACGCATTTCCACATCACACCTAA
AAATTGATCCGCTTATTGTA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MIHEVFMPALSSTMEEGKIVSWSKEPGDKVEKGETVLVVESDKADMDVESFHEGYLAAIAVPAGGVAKVGAAIGYVAETE
AEIAEAQKKASAAESAAPAPAAPAPAPAAPAPAAVAPAPPAAAPAPVATIPVAPAATLNGGSAPAAPSNGRVVVSPRARK
LAKQFKVDLNTLTGSGPHGRIVAADIEAASGQTSTTATAPAASSAAPQPSLPASAPLPAGAAAGEVVPFNTLQQAVVNNM
VASLAVPTFHVEYSIVTDALDQLYKQVKTKGVTMTALLAKAVAVTLRQHPLVNASCAPQGIQYSSAINIAVAVAMPGGGL
ITPVLQQADQMDLYSLSRTWRDLVARARSKQLQPDEYSTGTFTLSNLGMFGVNSFDAILPPGQGSILAIGGSKPQVVADD
QGMMGVKRLMNVNITCDHRVIYGADAAAFLKDLAELIETNPQSLTL

Sequences:

>Translated_446_residues
MIHEVFMPALSSTMEEGKIVSWSKEPGDKVEKGETVLVVESDKADMDVESFHEGYLAAIAVPAGGVAKVGAAIGYVAETE
AEIAEAQKKASAAESAAPAPAAPAPAPAAPAPAAVAPAPPAAAPAPVATIPVAPAATLNGGSAPAAPSNGRVVVSPRARK
LAKQFKVDLNTLTGSGPHGRIVAADIEAASGQTSTTATAPAASSAAPQPSLPASAPLPAGAAAGEVVPFNTLQQAVVNNM
VASLAVPTFHVEYSIVTDALDQLYKQVKTKGVTMTALLAKAVAVTLRQHPLVNASCAPQGIQYSSAINIAVAVAMPGGGL
ITPVLQQADQMDLYSLSRTWRDLVARARSKQLQPDEYSTGTFTLSNLGMFGVNSFDAILPPGQGSILAIGGSKPQVVADD
QGMMGVKRLMNVNITCDHRVIYGADAAAFLKDLAELIETNPQSLTL
>Mature_446_residues
MIHEVFMPALSSTMEEGKIVSWSKEPGDKVEKGETVLVVESDKADMDVESFHEGYLAAIAVPAGGVAKVGAAIGYVAETE
AEIAEAQKKASAAESAAPAPAAPAPAPAAPAPAAVAPAPPAAAPAPVATIPVAPAATLNGGSAPAAPSNGRVVVSPRARK
LAKQFKVDLNTLTGSGPHGRIVAADIEAASGQTSTTATAPAASSAAPQPSLPASAPLPAGAAAGEVVPFNTLQQAVVNNM
VASLAVPTFHVEYSIVTDALDQLYKQVKTKGVTMTALLAKAVAVTLRQHPLVNASCAPQGIQYSSAINIAVAVAMPGGGL
ITPVLQQADQMDLYSLSRTWRDLVARARSKQLQPDEYSTGTFTLSNLGMFGVNSFDAILPPGQGSILAIGGSKPQVVADD
QGMMGVKRLMNVNITCDHRVIYGADAAAFLKDLAELIETNPQSLTL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=447, Percent_Identity=32.4384787472036, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI203098816, Length=480, Percent_Identity=27.5, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI203098753, Length=480, Percent_Identity=27.5, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=24.3362831858407, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI260898739, Length=193, Percent_Identity=34.7150259067358, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI19923748, Length=173, Percent_Identity=34.6820809248555, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=452, Percent_Identity=24.3362831858407, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786305, Length=309, Percent_Identity=30.42071197411, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560088, Length=463, Percent_Identity=34.341252699784, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=25.2821670428894, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=213, Percent_Identity=35.2112676056338, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25146366, Length=445, Percent_Identity=28.0898876404494, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=32.8227571115974, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320352, Length=186, Percent_Identity=32.7956989247312, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=187, Percent_Identity=29.9465240641711, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129315, Length=452, Percent_Identity=33.8495575221239, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24582497, Length=445, Percent_Identity=33.2584269662921, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18859875, Length=323, Percent_Identity=30.6501547987616, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24645909, Length=184, Percent_Identity=33.1521739130435, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45427; Mature: 45427

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHEVFMPALSSTMEEGKIVSWSKEPGDKVEKGETVLVVESDKADMDVESFHEGYLAAIA
CCHHHHHHHHHHHHHCCEEEEECCCCCCHHCCCCEEEEEECCCCCCCHHHHHCCEEEEEE
VPAGGVAKVGAAIGYVAETEAEIAEAQKKASAAESAAPAPAAPAPAPAAPAPAAVAPAPP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
AAAPAPVATIPVAPAATLNGGSAPAAPSNGRVVVSPRARKLAKQFKVDLNTLTGSGPHGR
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHEECCCCCCCC
IVAADIEAASGQTSTTATAPAASSAAPQPSLPASAPLPAGAAAGEVVPFNTLQQAVVNNM
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VASLAVPTFHVEYSIVTDALDQLYKQVKTKGVTMTALLAKAVAVTLRQHPLVNASCAPQG
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IQYSSAINIAVAVAMPGGGLITPVLQQADQMDLYSLSRTWRDLVARARSKQLQPDEYSTG
CEECCEEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TFTLSNLGMFGVNSFDAILPPGQGSILAIGGSKPQVVADDQGMMGVKRLMNVNITCDHRV
EEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHHHCCCEEECCEE
IYGADAAAFLKDLAELIETNPQSLTL
EECCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIHEVFMPALSSTMEEGKIVSWSKEPGDKVEKGETVLVVESDKADMDVESFHEGYLAAIA
CCHHHHHHHHHHHHHCCEEEEECCCCCCHHCCCCEEEEEECCCCCCCHHHHHCCEEEEEE
VPAGGVAKVGAAIGYVAETEAEIAEAQKKASAAESAAPAPAAPAPAPAAPAPAAVAPAPP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
AAAPAPVATIPVAPAATLNGGSAPAAPSNGRVVVSPRARKLAKQFKVDLNTLTGSGPHGR
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHEECCCCCCCC
IVAADIEAASGQTSTTATAPAASSAAPQPSLPASAPLPAGAAAGEVVPFNTLQQAVVNNM
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VASLAVPTFHVEYSIVTDALDQLYKQVKTKGVTMTALLAKAVAVTLRQHPLVNASCAPQG
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IQYSSAINIAVAVAMPGGGLITPVLQQADQMDLYSLSRTWRDLVARARSKQLQPDEYSTG
CEECCEEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TFTLSNLGMFGVNSFDAILPPGQGSILAIGGSKPQVVADDQGMMGVKRLMNVNITCDHRV
EEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHHHCCCEEECCEE
IYGADAAAFLKDLAELIETNPQSLTL
EECCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]