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The map label for this gene is dnaA

Identifier: 158336233

GI number: 158336233

Start: 3124142

End: 3125509

Strand: Direct

Name: dnaA

Synonym: AM1_3095

Alternate gene names: 158336233

Gene position: 3124142-3125509 (Clockwise)

Preceding gene: 158336227

Following gene: 158336234

Centisome position: 48.04

GC content: 47.51

Gene sequence:

>1368_bases
ATGGAAACTTCTCTTGAAACTCTTTGGAGCCAAGTTTTAGAACGCTTGCAGCTGCAGCTCAGCCGCCCCACCTTCGAAAC
TTGGATTAAAACCGCCAATGCTGAACAGCTAGATGAGAATCGCCTAGTGATTCGCACGCCTAATCCGTTTGCCCGGAATT
GGTTGCAGAAATACTATGTCAAAACCATTCGAGATGTCGTCCATGAGATTTTGGGGCATCCTGTCGAAATTCAAATTGAA
ATTGCCCAAGGGGATTCCAACGCTACGATTTCAGCCCCGGAGGTTGCCTCACCACCGCCCACCGCTAGCCCAGTTGAAAA
TACCAACACTAGCCAACGCCAACAAGCCAGCCTCAATCCTAAATATGTGTTTTCTCGCTATGTGGTCGGCCCCAATAATC
GCATGGCCCATGCCGCTTGTTTAGCGGTTGCTGAGTCTCCAGGGCGCGAATTTAATCCATTATTTCTCTGTGGCGGTGTT
GGATTGGGAAAAACCCACCTAATGCAGGCCATCGGCCATTACCGACTAGAGATTTCACCGAACTCACGTATTTTCTACAT
CTCCACTGAACAATTCACCAATGATCTGATTGCCGCCATTCGCAAGGATGGAATGCAAAAGTTTCGAGAACATTACCGAG
CAGTAGATGTGATGTTGGTCGATGACATCCAATTTATTGAAGGCAAAGAATATACCCAAGAAGAGTTTTTTCATACCTTC
AATACATTGCATGAGGCAGGCAAGCAGGTTGTACTCGCGTCTGACCGTCCACCCAGCCAAATTCCACGGCTACAGGAGCG
TCTCTGTTCCCGCTTTTCCATGGGATTGATTGCAGATATTCAGCCCCCTGATTTAGAAACACGGATGGCCATTCTGCAAA
AGAAAGCAGAATACGAAAATATTCGCCTTCCTAGAGAAGTCATTGAATATATTGCGTCTAGCTACACCTCTAACATTCGA
GAACTCGAAGGCGCTCTCATTCGGGCAGTGGCCTATATTTCCATTTCTGGACTGCCCATGAATGTGGAAAATATCGCTCC
AGTACTCAATCCACCGACTGCCAAAATATCGGCTTCCCCTGAAAGCATTATTAACGCCGTCGCTGATACCTATGGCATCT
CGATTGATGATCTCAAAGGCAATTCCCGACGCCGGGAAATTAGCATGGCCCGCCAAATTGGCATGTACCTTATGCGCCAG
CATACCGACCTCAGCCTGCCAAAAATTGGCGAGGAATTCGGTGGCAAAGACCACACCACGGTTATGTATAGCTGTGACAA
AGTATCAGACTTACAAAAGAAAAATCCAGAACTCGCCCAATCTCTACGACAGTTAGGTGATCGGATCAAGCTGGCCAACC
AACCCTAA

Upstream 100 bases:

>100_bases
TGACCCAGAATCCATAGTTTACAATCTTATCACCCACTCTTGCCAGACCGAATGAAACCCCTTACGCTCAATGTTGCTCA
GCTTGCTGTCGAGGTAATCC

Downstream 100 bases:

>100_bases
GGGGAAGCCAGCTTGTTATTCATCTCTCCCGAATGGGAAGAGGACGAAGTGCTGCCATCCAGTGATATTGAACTCTGGTA
GTCATTGCTATACCAGAGGA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
METSLETLWSQVLERLQLQLSRPTFETWIKTANAEQLDENRLVIRTPNPFARNWLQKYYVKTIRDVVHEILGHPVEIQIE
IAQGDSNATISAPEVASPPPTASPVENTNTSQRQQASLNPKYVFSRYVVGPNNRMAHAACLAVAESPGREFNPLFLCGGV
GLGKTHLMQAIGHYRLEISPNSRIFYISTEQFTNDLIAAIRKDGMQKFREHYRAVDVMLVDDIQFIEGKEYTQEEFFHTF
NTLHEAGKQVVLASDRPPSQIPRLQERLCSRFSMGLIADIQPPDLETRMAILQKKAEYENIRLPREVIEYIASSYTSNIR
ELEGALIRAVAYISISGLPMNVENIAPVLNPPTAKISASPESIINAVADTYGISIDDLKGNSRRREISMARQIGMYLMRQ
HTDLSLPKIGEEFGGKDHTTVMYSCDKVSDLQKKNPELAQSLRQLGDRIKLANQP

Sequences:

>Translated_455_residues
METSLETLWSQVLERLQLQLSRPTFETWIKTANAEQLDENRLVIRTPNPFARNWLQKYYVKTIRDVVHEILGHPVEIQIE
IAQGDSNATISAPEVASPPPTASPVENTNTSQRQQASLNPKYVFSRYVVGPNNRMAHAACLAVAESPGREFNPLFLCGGV
GLGKTHLMQAIGHYRLEISPNSRIFYISTEQFTNDLIAAIRKDGMQKFREHYRAVDVMLVDDIQFIEGKEYTQEEFFHTF
NTLHEAGKQVVLASDRPPSQIPRLQERLCSRFSMGLIADIQPPDLETRMAILQKKAEYENIRLPREVIEYIASSYTSNIR
ELEGALIRAVAYISISGLPMNVENIAPVLNPPTAKISASPESIINAVADTYGISIDDLKGNSRRREISMARQIGMYLMRQ
HTDLSLPKIGEEFGGKDHTTVMYSCDKVSDLQKKNPELAQSLRQLGDRIKLANQP
>Mature_455_residues
METSLETLWSQVLERLQLQLSRPTFETWIKTANAEQLDENRLVIRTPNPFARNWLQKYYVKTIRDVVHEILGHPVEIQIE
IAQGDSNATISAPEVASPPPTASPVENTNTSQRQQASLNPKYVFSRYVVGPNNRMAHAACLAVAESPGREFNPLFLCGGV
GLGKTHLMQAIGHYRLEISPNSRIFYISTEQFTNDLIAAIRKDGMQKFREHYRAVDVMLVDDIQFIEGKEYTQEEFFHTF
NTLHEAGKQVVLASDRPPSQIPRLQERLCSRFSMGLIADIQPPDLETRMAILQKKAEYENIRLPREVIEYIASSYTSNIR
ELEGALIRAVAYISISGLPMNVENIAPVLNPPTAKISASPESIINAVADTYGISIDDLKGNSRRREISMARQIGMYLMRQ
HTDLSLPKIGEEFGGKDHTTVMYSCDKVSDLQKKNPELAQSLRQLGDRIKLANQP

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:
GO:0000166: Chromosomal replication initiator protein DnaA
GO:0003677: Chromosomal replication initiator protein DnaA
GO:0003688: Chromosomal replication initiator protein DnaA
GO:0005524: Chromosomal replication initiator protein DnaA
GO:0005737: Chromosomal replication initiator protein DnaA
GO:0006260: Chromosomal replication initiator protein DnaA
GO:0006270: Chromosomal replication initiator protein DnaA
GO:0006275: Chromosomal replication initiator protein DnaA
GO:0017111: Chromosomal replication initiator protein DnaA
GO:0043565: Chromosomal replication initiator protein DnaA

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=459, Percent_Identity=37.6906318082789, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_ACAM1 (B0CDM2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517407.1
- ProteinModelPortal:   B0CDM2
- SMR:   B0CDM2
- GeneID:   5681903
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3095
- HOGENOM:   HBG695666
- OMA:   WPLCLRR
- ProtClustDB:   PRK00149
- BioCyc:   AMAR329726:AM1_3095-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51336; Mature: 51336

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSLETLWSQVLERLQLQLSRPTFETWIKTANAEQLDENRLVIRTPNPFARNWLQKYYV
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCEEEEECCCHHHHHHHHHHHH
KTIRDVVHEILGHPVEIQIEIAQGDSNATISAPEVASPPPTASPVENTNTSQRQQASLNP
HHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
KYVFSRYVVGPNNRMAHAACLAVAESPGREFNPLFLCGGVGLGKTHLMQAIGHYRLEISP
HEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEEEECC
NSRIFYISTEQFTNDLIAAIRKDGMQKFREHYRAVDVMLVDDIQFIEGKEYTQEEFFHTF
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHHH
NTLHEAGKQVVLASDRPPSQIPRLQERLCSRFSMGLIADIQPPDLETRMAILQKKAEYEN
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHH
IRLPREVIEYIASSYTSNIRELEGALIRAVAYISISGLPMNVENIAPVLNPPTAKISASP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCEECCCH
ESIINAVADTYGISIDDLKGNSRRREISMARQIGMYLMRQHTDLSLPKIGEEFGGKDHTT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCE
VMYSCDKVSDLQKKNPELAQSLRQLGDRIKLANQP
EEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METSLETLWSQVLERLQLQLSRPTFETWIKTANAEQLDENRLVIRTPNPFARNWLQKYYV
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCEEEEECCCHHHHHHHHHHHH
KTIRDVVHEILGHPVEIQIEIAQGDSNATISAPEVASPPPTASPVENTNTSQRQQASLNP
HHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
KYVFSRYVVGPNNRMAHAACLAVAESPGREFNPLFLCGGVGLGKTHLMQAIGHYRLEISP
HEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEEEECC
NSRIFYISTEQFTNDLIAAIRKDGMQKFREHYRAVDVMLVDDIQFIEGKEYTQEEFFHTF
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHHH
NTLHEAGKQVVLASDRPPSQIPRLQERLCSRFSMGLIADIQPPDLETRMAILQKKAEYEN
HHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHH
IRLPREVIEYIASSYTSNIRELEGALIRAVAYISISGLPMNVENIAPVLNPPTAKISASP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCEECCCH
ESIINAVADTYGISIDDLKGNSRRREISMARQIGMYLMRQHTDLSLPKIGEEFGGKDHTT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCE
VMYSCDKVSDLQKKNPELAQSLRQLGDRIKLANQP
EEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA