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The map label for this gene is dnaN [H]

Identifier: 158336234

GI number: 158336234

Start: 3126136

End: 3127254

Strand: Direct

Name: dnaN [H]

Synonym: AM1_3096

Alternate gene names: 158336234

Gene position: 3126136-3127254 (Clockwise)

Preceding gene: 158336233

Following gene: 158336235

Centisome position: 48.07

GC content: 47.81

Gene sequence:

>1119_bases
ATGAAGTTGGTCTGTCAGCAAAGTGATCTAAATTCCAAACTGTCACTCCTTAGTCGAGTAGTCCCTTCGAATCCTACCCA
CCCGGTTCTAGCGAATGTTTTATTGACGGCTCAAGCCGAACGCCTCGGTTTAACCGTGTTTGATTTGAGTTTAGGAATTC
AAGTTTGGATTGCTGCAGATGTCGAAGAAACCGGCGCTTTAACGTTGCCAGCTCGGTTTCTCAACGATATTGTCTCGCGT
TTACCCAACTGCGATATCGAAGTCGAAACCGATGACACAGCTGTAACATTGACCTGCGATTCAGGCCATTACCAAATGCA
AGGATTAGTGGCGGAAGAATTTCCAGGACTGCCGAGTTTGGATGAAATACCCGCCCTAGATCTGCCCACAGACGTGTTTC
TTGAGGGATTGCAGGCCACTTTGTTTGCAGCGAGTACTGACGAAAGCAAACAAATTCTGACGGGGCTGCATCTGAATACT
TCAGAGTCAGGGTTTGAATTTGCAACAACGGATGGGCATCGACTTGCGATCGCAAACTTTCCTGACCTCGTCACTCCAGA
GCCCATGACCATGACCATACCTGCGAAAGCCTTACGAGAGCTAGAGCGGATGCTGGGAAGAGTCAATGCCGATGTAGCCC
TACGATTTGATACCAATCAGGCCATTTTTGAACTAATCGGCGAACAAGGAACGGAGCGCCTGAGCTGTCGATTGTTGGAA
GGGCAATATCCAGCCTATCCTCAACTTGTGCCGAAGCAGTTTGAGCGGCAAGTGACAGTGGAGCGGCAGCTGTTGCTCAG
TAGTGTGGAACGGATTGCAGTATTAGCCGCCCGCAAAAATAATATTATTCGTTTAAAGATCGATAGTGCTGAACAGAATG
TGGCTCTGTCAGCAGAGGCGCCTGAGTTGGGTATGGGAGAGGAACGTTTGCCAGCTCAAATCTCAGGAGAAGATTTAGAC
ATCGCCTTTAATGTGAAATATTTGAGTGATGGCTTAAAGGCACTGGATAGCCAAGAAGTACAGCTGCAAATGAATTCGGC
GACTATGCCAGCGGTTTTATCTCCCTTGTCGGGTCGTCAAATTACCTATTTGATCATGCCAATTCAAATTCGCGGTTAG

Upstream 100 bases:

>100_bases
TGATTTTTGACCAGCATTTCTTGCAACTTTGAGGGACAGGGCAATGATGTTACGATGTTGCTCCCAGAGTTTAGAAAGTG
TTGAAGTCCCTAGAAGCCTT

Downstream 100 bases:

>100_bases
CCTAGCCCATCAATCATGGGTATCTTAAGTAATATGGGGAACTATTTAAATTGATCCATGATCTAAAGTATGAGTGAGTA
GACTATTTATATTGCTGACG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKLVCQQSDLNSKLSLLSRVVPSNPTHPVLANVLLTAQAERLGLTVFDLSLGIQVWIAADVEETGALTLPARFLNDIVSR
LPNCDIEVETDDTAVTLTCDSGHYQMQGLVAEEFPGLPSLDEIPALDLPTDVFLEGLQATLFAASTDESKQILTGLHLNT
SESGFEFATTDGHRLAIANFPDLVTPEPMTMTIPAKALRELERMLGRVNADVALRFDTNQAIFELIGEQGTERLSCRLLE
GQYPAYPQLVPKQFERQVTVERQLLLSSVERIAVLAARKNNIIRLKIDSAEQNVALSAEAPELGMGEERLPAQISGEDLD
IAFNVKYLSDGLKALDSQEVQLQMNSATMPAVLSPLSGRQITYLIMPIQIRG

Sequences:

>Translated_372_residues
MKLVCQQSDLNSKLSLLSRVVPSNPTHPVLANVLLTAQAERLGLTVFDLSLGIQVWIAADVEETGALTLPARFLNDIVSR
LPNCDIEVETDDTAVTLTCDSGHYQMQGLVAEEFPGLPSLDEIPALDLPTDVFLEGLQATLFAASTDESKQILTGLHLNT
SESGFEFATTDGHRLAIANFPDLVTPEPMTMTIPAKALRELERMLGRVNADVALRFDTNQAIFELIGEQGTERLSCRLLE
GQYPAYPQLVPKQFERQVTVERQLLLSSVERIAVLAARKNNIIRLKIDSAEQNVALSAEAPELGMGEERLPAQISGEDLD
IAFNVKYLSDGLKALDSQEVQLQMNSATMPAVLSPLSGRQITYLIMPIQIRG
>Mature_372_residues
MKLVCQQSDLNSKLSLLSRVVPSNPTHPVLANVLLTAQAERLGLTVFDLSLGIQVWIAADVEETGALTLPARFLNDIVSR
LPNCDIEVETDDTAVTLTCDSGHYQMQGLVAEEFPGLPSLDEIPALDLPTDVFLEGLQATLFAASTDESKQILTGLHLNT
SESGFEFATTDGHRLAIANFPDLVTPEPMTMTIPAKALRELERMLGRVNADVALRFDTNQAIFELIGEQGTERLSCRLLE
GQYPAYPQLVPKQFERQVTVERQLLLSSVERIAVLAARKNNIIRLKIDSAEQNVALSAEAPELGMGEERLPAQISGEDLD
IAFNVKYLSDGLKALDSQEVQLQMNSATMPAVLSPLSGRQITYLIMPIQIRG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:
GO:0003677: DNA polymerase III, beta subunit
GO:0003887: DNA polymerase III, beta subunit
GO:0005737: DNA polymerase III, beta subunit
GO:0006260: DNA polymerase III, beta subunit
GO:0008408: DNA polymerase III, beta subunit
GO:0009360: DNA polymerase III, beta subunit
GO:0016740: DNA polymerase III, beta subunit
GO:0016779: DNA polymerase III, beta subunit

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=372, Percent_Identity=26.0752688172043, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40706; Mature: 40706

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVCQQSDLNSKLSLLSRVVPSNPTHPVLANVLLTAQAERLGLTVFDLSLGIQVWIAAD
CCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCEEEEEEECCEEEEEEEC
VEETGALTLPARFLNDIVSRLPNCDIEVETDDTAVTLTCDSGHYQMQGLVAEEFPGLPSL
CCCCCCEEECHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEECEEHHHCCCCCCH
DEIPALDLPTDVFLEGLQATLFAASTDESKQILTGLHLNTSESGFEFATTDGHRLAIANF
HCCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHEEEEECCCCCCCEEEECCCCEEEEECC
PDLVTPEPMTMTIPAKALRELERMLGRVNADVALRFDTNQAIFELIGEQGTERLSCRLLE
CCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEC
GQYPAYPQLVPKQFERQVTVERQLLLSSVERIAVLAARKNNIIRLKIDSAEQNVALSAEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCEEEECCC
PELGMGEERLPAQISGEDLDIAFNVKYLSDGLKALDSQEVQLQMNSATMPAVLSPLSGRQ
CCCCCCCCCCCCEECCCCEEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCE
ITYLIMPIQIRG
EEEEEEEEEECC
>Mature Secondary Structure
MKLVCQQSDLNSKLSLLSRVVPSNPTHPVLANVLLTAQAERLGLTVFDLSLGIQVWIAAD
CCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCEEEEEEECCEEEEEEEC
VEETGALTLPARFLNDIVSRLPNCDIEVETDDTAVTLTCDSGHYQMQGLVAEEFPGLPSL
CCCCCCEEECHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEECEEHHHCCCCCCH
DEIPALDLPTDVFLEGLQATLFAASTDESKQILTGLHLNTSESGFEFATTDGHRLAIANF
HCCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHEEEEECCCCCCCEEEECCCCEEEEECC
PDLVTPEPMTMTIPAKALRELERMLGRVNADVALRFDTNQAIFELIGEQGTERLSCRLLE
CCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEC
GQYPAYPQLVPKQFERQVTVERQLLLSSVERIAVLAARKNNIIRLKIDSAEQNVALSAEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCEEEECCC
PELGMGEERLPAQISGEDLDIAFNVKYLSDGLKALDSQEVQLQMNSATMPAVLSPLSGRQ
CCCCCCCCCCCCEECCCCEEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCE
ITYLIMPIQIRG
EEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]