Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 158335615

GI number: 158335615

Start: 2486148

End: 2487488

Strand: Reverse

Name: pmbA [H]

Synonym: AM1_2465

Alternate gene names: 158335615

Gene position: 2487488-2486148 (Counterclockwise)

Preceding gene: 158335616

Following gene: 158335611

Centisome position: 38.25

GC content: 51.3

Gene sequence:

>1341_bases
GTGTCTGATGTTGCTACCATCGCTGCGATCGCAAAAGATAAAGCCCAAGCCTTAGGCATCCAAAAGTTTGATGTCTGTGG
GTCTACCGTTGATGAAGCCAGCGTCCAAGTCGATCAAGGTGAACCCAAACAGGTGAAAGCCTCCAAGCGCTCCAGTGTTA
TGGTCCGCGTCTGGAATGAAGCCAATACCGTCGGCGTCACCACCACCAGCGAAGTGGATGAACAGGGACTCGAACTCGCC
CTCAAAACGGCCCAAGAAGCCAGTGCCTTTGGGGCCAAAGAGTATGTGCCGGACTTCAGTCCAGAAGCCACTGCTGCTCT
AGACACCACTCAATCTGAACTGGTGCCCCAGGCGGAGATTGCCACCCTGATCGATAACTTGGTGCAAACCGAAGCGAAGT
TAATGGACGCCCATGATGCCATTTCAGGTGTTCCTTATAATGGCCTTGCCCAACGAGATGCCCAGCGGTTCTACCTCAAC
AGTGAAGGGGCCATGCGCCAAGAAGCTCGGTCATCGAGTTCGGTGTATCTCTACAGCGTGACGGAGCAGGAAGGTAAACG
TCCCCGTAGTGCTGGCGCATATCGAGTCAGCCAAGGTCTAGATCAGCTCGACATTGAGGGGTGTTTGCAAGAAACGGCAG
AGAAAACTATCAGCCACCTCAACTACCAGCCGATTACGTCTGGCAAGTATCGTGTTGTCCTGTCTGCCGAAGCGTTCCTG
AGTTTGTTAGGTGCCTTCTCTAACCTATTCAATGCCCAAAGCATACTCGACAACCGCAGCCTAGCGACGGTTGAGTCCCT
AGGAACGGAAATTGCATCCCCCCTACTTTCCGTTGCCGATGATGCCCTCCATGCTGACAATGTGGGCAAAACCACTTTCG
ACGATGAAGGAACCCCTACACGACGCATTCCAATTATTGATAAAGGAGTCCTCACTCATTTCCTTCATAGTTCAGGAACG
GCTAAGCGTCTTGATGCTCAACCCACGGGTCATGCCAATCTAGGAGCTAAGGTCACCGTGGGGCCACATTTCTACCATGT
GTTTGCTGCCAGTACCCCTGAGCAAACTTACAGCTTAGAGACTGCCGAAAATGTTGTCTTTATTGATGATGTTCAAGCCC
TGCATGCTGGCGTCAAAGCTCTGCAAGGCTCTTTCTCTCTACCTTTCGATGGTTGGCTTGTTAATAAAGGGGAGAAAATC
AGTATCGAATCTGCCACCGTTGCCGGTGATTTTCGAGATCTGCTCACATCAATTATCTATGTTGAATCTGAGGCAGAACT
CACGCCTGGTGGCATCTGCCCCAGAATCTGGGTGGATGAGTTATCCATTACGGGTGAATAG

Upstream 100 bases:

>100_bases
ATGAGATTACCGTGGGTGGTCGTTAAATCGTCACTGCCTACAAGCTATTACACCTATAAATTCCAGAGAATACACCTAAA
AAATAGGAGAACCCGTTAGC

Downstream 100 bases:

>100_bases
TGACTCTCGCCTGATCACCACCGTCCAAATGGCAGCCAGGATGACTGTTCTTTCAGCGATTAGATGACATCTTGCTGTTT
CAAAATCACGAGGATAAAGC

Product: modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSDVATIAAIAKDKAQALGIQKFDVCGSTVDEASVQVDQGEPKQVKASKRSSVMVRVWNEANTVGVTTTSEVDEQGLELA
LKTAQEASAFGAKEYVPDFSPEATAALDTTQSELVPQAEIATLIDNLVQTEAKLMDAHDAISGVPYNGLAQRDAQRFYLN
SEGAMRQEARSSSSVYLYSVTEQEGKRPRSAGAYRVSQGLDQLDIEGCLQETAEKTISHLNYQPITSGKYRVVLSAEAFL
SLLGAFSNLFNAQSILDNRSLATVESLGTEIASPLLSVADDALHADNVGKTTFDDEGTPTRRIPIIDKGVLTHFLHSSGT
AKRLDAQPTGHANLGAKVTVGPHFYHVFAASTPEQTYSLETAENVVFIDDVQALHAGVKALQGSFSLPFDGWLVNKGEKI
SIESATVAGDFRDLLTSIIYVESEAELTPGGICPRIWVDELSITGE

Sequences:

>Translated_446_residues
MSDVATIAAIAKDKAQALGIQKFDVCGSTVDEASVQVDQGEPKQVKASKRSSVMVRVWNEANTVGVTTTSEVDEQGLELA
LKTAQEASAFGAKEYVPDFSPEATAALDTTQSELVPQAEIATLIDNLVQTEAKLMDAHDAISGVPYNGLAQRDAQRFYLN
SEGAMRQEARSSSSVYLYSVTEQEGKRPRSAGAYRVSQGLDQLDIEGCLQETAEKTISHLNYQPITSGKYRVVLSAEAFL
SLLGAFSNLFNAQSILDNRSLATVESLGTEIASPLLSVADDALHADNVGKTTFDDEGTPTRRIPIIDKGVLTHFLHSSGT
AKRLDAQPTGHANLGAKVTVGPHFYHVFAASTPEQTYSLETAENVVFIDDVQALHAGVKALQGSFSLPFDGWLVNKGEKI
SIESATVAGDFRDLLTSIIYVESEAELTPGGICPRIWVDELSITGE
>Mature_445_residues
SDVATIAAIAKDKAQALGIQKFDVCGSTVDEASVQVDQGEPKQVKASKRSSVMVRVWNEANTVGVTTTSEVDEQGLELAL
KTAQEASAFGAKEYVPDFSPEATAALDTTQSELVPQAEIATLIDNLVQTEAKLMDAHDAISGVPYNGLAQRDAQRFYLNS
EGAMRQEARSSSSVYLYSVTEQEGKRPRSAGAYRVSQGLDQLDIEGCLQETAEKTISHLNYQPITSGKYRVVLSAEAFLS
LLGAFSNLFNAQSILDNRSLATVESLGTEIASPLLSVADDALHADNVGKTTFDDEGTPTRRIPIIDKGVLTHFLHSSGTA
KRLDAQPTGHANLGAKVTVGPHFYHVFAASTPEQTYSLETAENVVFIDDVQALHAGVKALQGSFSLPFDGWLVNKGEKIS
IESATVAGDFRDLLTSIIYVESEAELTPGGICPRIWVDELSITGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=417, Percent_Identity=21.5827338129496, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47848; Mature: 47717

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVATIAAIAKDKAQALGIQKFDVCGSTVDEASVQVDQGEPKQVKASKRSSVMVRVWNE
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCEEEEEECC
ANTVGVTTTSEVDEQGLELALKTAQEASAFGAKEYVPDFSPEATAALDTTQSELVPQAEI
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCCHHHHCCHHHH
ATLIDNLVQTEAKLMDAHDAISGVPYNGLAQRDAQRFYLNSEGAMRQEARSSSSVYLYSV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHEEECCCCHHHHHHCCCCCEEEEEE
TEQEGKRPRSAGAYRVSQGLDQLDIEGCLQETAEKTISHLNYQPITSGKYRVVLSAEAFL
CHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
SLLGAFSNLFNAQSILDNRSLATVESLGTEIASPLLSVADDALHADNVGKTTFDDEGTPT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRIPIIDKGVLTHFLHSSGTAKRLDAQPTGHANLGAKVTVGPHFYHVFAASTPEQTYSLE
CCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHEEEE
TAENVVFIDDVQALHAGVKALQGSFSLPFDGWLVNKGEKISIESATVAGDFRDLLTSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECEEEHHHHHHHHHHHEE
VESEAELTPGGICPRIWVDELSITGE
ECCCCCCCCCCCCCHHEEECEEECCC
>Mature Secondary Structure 
SDVATIAAIAKDKAQALGIQKFDVCGSTVDEASVQVDQGEPKQVKASKRSSVMVRVWNE
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCEEEEEECC
ANTVGVTTTSEVDEQGLELALKTAQEASAFGAKEYVPDFSPEATAALDTTQSELVPQAEI
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCCHHHHCCHHHH
ATLIDNLVQTEAKLMDAHDAISGVPYNGLAQRDAQRFYLNSEGAMRQEARSSSSVYLYSV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHEEECCCCHHHHHHCCCCCEEEEEE
TEQEGKRPRSAGAYRVSQGLDQLDIEGCLQETAEKTISHLNYQPITSGKYRVVLSAEAFL
CHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
SLLGAFSNLFNAQSILDNRSLATVESLGTEIASPLLSVADDALHADNVGKTTFDDEGTPT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRIPIIDKGVLTHFLHSSGTAKRLDAQPTGHANLGAKVTVGPHFYHVFAASTPEQTYSLE
CCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHEEEE
TAENVVFIDDVQALHAGVKALQGSFSLPFDGWLVNKGEKISIESATVAGDFRDLLTSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECEEEHHHHHHHHHHHEE
VESEAELTPGGICPRIWVDELSITGE
ECCCCCCCCCCCCCHHEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]