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The map label for this gene is exsB

Identifier: 158335496

GI number: 158335496

Start: 2347587

End: 2348273

Strand: Reverse

Name: exsB

Synonym: AM1_2344

Alternate gene names: 158335496

Gene position: 2348273-2347587 (Counterclockwise)

Preceding gene: 158335498

Following gene: 158335495

Centisome position: 36.11

GC content: 52.26

Gene sequence:

>687_bases
GTGAAAGCAGTTGTTTTATTGTCAGGTGGGCTAGACTCGTCCACTGCTTTATACAAAGCAAAAGCCGATGGGGCTGACTG
TTATGCCATCTCGTTTGACTATCGGCAGCGTCATCGCCAGGAATTGGATTCGGCGGTTGCGATCGCACAATCAGCCCAAG
TCACTCAACATCAAATTGTGGCCTTCGACCTCACCCTCTGGGGTGGGTCTGCCTTAACCGATACCCAAATCGACTTGCCG
TCTCGTTCCATCGAAGAGATGGCGGATCATATTCCCGTCACTTACGTGCCCGCCCGCAACTCGATCTTCCTTAGCTTTGC
CCTCGCCTATGCAGAAACGATTTCAGCAGAGCAGGTCTATATCGGAGTTAACCAACTGGATTACTCGGGATATCCCGACT
GTCGCCCGGACTTTATCCAAGCCATGCAAGAGGTGTTTCGGTTAGGGACCAAGCTGGGTCGGGAGGGGCAGGCTATCGAA
ATTTGTACCCCTCTGATCAATTTGCATAAGTCTGCCATTATCGAATTGGGCAATGACCTGGGTGTACCGTGGGAGCAGAC
CTGGTCTTGCTACAGTGACGGTGGCGGCTCACCACCCCTAGCCTGTGGTCAGTGTGATTCCTGCCAATTACGATTAGCGG
CATTTGCTCAACTCGGTTTATCCGATCCCCTATCCTATGCCACCTAA

Upstream 100 bases:

>100_bases
TGTCCTGAGCAACCCATCGCTAATTCTCATGTGTCACTCATTTCTCTATGGCAAGTATCCAACTTAGGATTTATAACGAC
GATAGTCAGGGAGATGGGAT

Downstream 100 bases:

>100_bases
CCGCAACATCGACCTCTATTTTCCTCCGATGGGGTTGATCGCCACTCCCCTAAGCATTTGCCTTTATGAAAACTGCATCC
ACCGCCAACTTAGTTGAGAT

Product: ExsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKAVVLLSGGLDSSTALYKAKADGADCYAISFDYRQRHRQELDSAVAIAQSAQVTQHQIVAFDLTLWGGSALTDTQIDLP
SRSIEEMADHIPVTYVPARNSIFLSFALAYAETISAEQVYIGVNQLDYSGYPDCRPDFIQAMQEVFRLGTKLGREGQAIE
ICTPLINLHKSAIIELGNDLGVPWEQTWSCYSDGGGSPPLACGQCDSCQLRLAAFAQLGLSDPLSYAT

Sequences:

>Translated_228_residues
MKAVVLLSGGLDSSTALYKAKADGADCYAISFDYRQRHRQELDSAVAIAQSAQVTQHQIVAFDLTLWGGSALTDTQIDLP
SRSIEEMADHIPVTYVPARNSIFLSFALAYAETISAEQVYIGVNQLDYSGYPDCRPDFIQAMQEVFRLGTKLGREGQAIE
ICTPLINLHKSAIIELGNDLGVPWEQTWSCYSDGGGSPPLACGQCDSCQLRLAAFAQLGLSDPLSYAT
>Mature_228_residues
MKAVVLLSGGLDSSTALYKAKADGADCYAISFDYRQRHRQELDSAVAIAQSAQVTQHQIVAFDLTLWGGSALTDTQIDLP
SRSIEEMADHIPVTYVPARNSIFLSFALAYAETISAEQVYIGVNQLDYSGYPDCRPDFIQAMQEVFRLGTKLGREGQAIE
ICTPLINLHKSAIIELGNDLGVPWEQTWSCYSDGGGSPPLACGQCDSCQLRLAAFAQLGLSDPLSYAT

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=214, Percent_Identity=36.4485981308411, Blast_Score=130, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_ACAM1 (B0C253)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516668.1
- ProteinModelPortal:   B0C253
- SMR:   B0C253
- GeneID:   5681157
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2344
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- ProtClustDB:   CLSK896323
- BioCyc:   AMAR329726:AM1_2344-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24735; Mature: 24735

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVVLLSGGLDSSTALYKAKADGADCYAISFDYRQRHRQELDSAVAIAQSAQVTQHQIV
CCEEEEEECCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEE
AFDLTLWGGSALTDTQIDLPSRSIEEMADHIPVTYVPARNSIFLSFALAYAETISAEQVY
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEE
IGVNQLDYSGYPDCRPDFIQAMQEVFRLGTKLGREGQAIEICTPLINLHKSAIIELGNDL
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCC
GVPWEQTWSCYSDGGGSPPLACGQCDSCQLRLAAFAQLGLSDPLSYAT
CCCHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAVVLLSGGLDSSTALYKAKADGADCYAISFDYRQRHRQELDSAVAIAQSAQVTQHQIV
CCEEEEEECCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEE
AFDLTLWGGSALTDTQIDLPSRSIEEMADHIPVTYVPARNSIFLSFALAYAETISAEQVY
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEE
IGVNQLDYSGYPDCRPDFIQAMQEVFRLGTKLGREGQAIEICTPLINLHKSAIIELGNDL
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCC
GVPWEQTWSCYSDGGGSPPLACGQCDSCQLRLAAFAQLGLSDPLSYAT
CCCHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA