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The map label for this gene is nuoL [H]

Identifier: 158335284

GI number: 158335284

Start: 2118910

End: 2120925

Strand: Reverse

Name: nuoL [H]

Synonym: AM1_2127

Alternate gene names: 158335284

Gene position: 2120925-2118910 (Counterclockwise)

Preceding gene: 158335293

Following gene: 158335283

Centisome position: 32.61

GC content: 49.7

Gene sequence:

>2016_bases
ATGACTGATTTTGCATACCTAATACCCTTATTTCCCTTGATTGCGTTTGCAGTCAATCTTTTCTTTGGTCATCGTTTAGG
TAGTTTCTCTGGATGGATTTCAGTCCTGACAAGTGTTGCCTCTTCGGCCATTGCTTTACCGGCAGCTTGGGTAGTCGCCC
AGGGCGGTCAGTACTCCCATGAGTGGCTGTGGTTGACCTTAGGAAATTACGACCTCAATTTTGGTTATTTACTCGATCCC
CTCTCAGCCACCTTGCTGTTTGTGGTGACAGTGATTGGTACCCTAATTCAGGTCTACTCCATTGGCTATATGCACGGTGA
GAAATACTATCACCGGTTTTTTGCCTATGTGTCCCTGTTCATGGCGGCCATGCTCACCTTGGTCATTGCCAATAACTATG
TGCAGTACTTTATGGCTTGGGAAGTCATGGGTCTCTGCTCCTATTTACTGATTGGTTTTTACTTCCATAAGGATTCAGCA
GCGAATGCCTCTCGGAAAGCCTTCTTAACCACCCGTGTTGGGGATGTGGGCTTATTTCTCGGCATTATGACCCTGTTTGT
GACCGGCGGTACCCTCAGCTTTGTGGATTTGCCGAATGCAGTAGAAACGGGTGCTCCGTTGCTGTGGCTGGCAGCTCCCC
TGATTTTCTGTGGAACCGTGGGTAAGTCGGCTCAATTTCCCTTGCACGTCTGGTTGCCAGACGCCATGGAAGGTCCGACG
CCAGTGAGTGCCCTGATTCATGCGGCAACCATGGTGGCTGCAGGGGTGTATCTGGTGGCTCGTTCCTTCGTGTTATTTAC
CAGCTTCCCTATGGTGATGCCCTGGGTGATGGGGATTGGCACCTTGACCGCGTTTATGGCGGCCTACATTGCTTTAACTG
CGACAGATATCAAGAAAGTCCTGGCTTTTTCCACGATTTCCCAGTTGGGTTACATGGTGACGGCCATGGGGCTGGGCGGT
ATGACCGCAGGCACCTTTCACCTGATGACCCACGCCTTCTTTAAGGCATTGCTCTTCCTCGGTGCGGGCAGCGTTATCCA
CGGCGCCCATGAACAGGACATCCGTCAATTAGGCGGCCTGTTCAGCAAGATGAAGCATACAGGGGCAACTTTTGTAATTG
CGTCCTTAGCCATTGCGGGTGTACCGCCTCTGTCTGGTTTCTGGTCTAAGGATGAAATCCTGCTAGAAGCCTATCAAGAA
GGCCATTTTGTGGTCTATGGCGTACTGTTGTTGACGGCCTTTATGACCGCCTTCTATATGTTCCGCCTGGTCTTTATGAC
CTTCTTTGGCAAAGCCCGTAACCCTGAGATTCATGCCCATGAGTCTCCCGCTTTGATGACTGTTCCCCTTTGGTGTCTTG
CCATTGGTGCCATCTTTGTGGGCATTCCAGGCTCCCCCTTTATGGATAACTGGTTTCAAACCTTCTTGACCGGTGAGCAT
CATGCAGCTGCAATTGACTGGATGGTGATGGGATCTTCGATTGCTGCTGGTGTTTTGGGAATTGGTCTAGCCGCAGTGCT
TTACCTCGCCAAGCCCCAATGGCCCGTTGCGATCGCAAATACGTTCCGTCCCCTTTACCTGTTATCGGTTAACCAATTCT
GGATTGACGATATCTATTCCGTCTTAATCCTCAATCCCTTTCAAGCCTTTGGAGAATTCCTCTTTGGGTTTGATCGCCTT
GTGATTGATGGCTTAGTCAACAGTTCAGCAACGGCCATGCAGAAAATGAGCAAAATCCAGCTCTGGATTGATCGCAACAT
TGTCGATGGGCTCGTTAATCTCACTGGCCGAGGCACCGTCATTGGCGGCGATATCCTCAAGCTGTTCCAAACGGGTTCTA
CCCAATTTTATTTACTGATTACTTGCGTTAGCGTTCTGGCCTTCCTGTCGGTTGAATTAACCTCAGGTTTCCAAGAACTT
CCTTTGCTCAGAGAGGAGTTGTATGGCATCTCATCTTTTATCCTTGATAATATTTTCCCCGCTCGTTGGGGCGGTCATAA
TCCTTTTGCTCCCTAA

Upstream 100 bases:

>100_bases
GATTCCACGGAGCAGTTTAGCTTGATGTTTAACCGCTACCGCCAGAATTAGGAAGACAGTTTGTGGGGATAAATTAATCG
ATCTCTTGAAATGGGAAACT

Downstream 100 bases:

>100_bases
GGCTCAAGAAAGCCTTTACAAATGGATATCTTTGGCCAATATGGTCGTGGTGAGTGCGCTTACATTTGCAGCATTCCTCC
AATTTGACCCGAATCAGACT

Product: proton-translocating NAD(P)H-quinone oxidoreductase, chain L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MTDFAYLIPLFPLIAFAVNLFFGHRLGSFSGWISVLTSVASSAIALPAAWVVAQGGQYSHEWLWLTLGNYDLNFGYLLDP
LSATLLFVVTVIGTLIQVYSIGYMHGEKYYHRFFAYVSLFMAAMLTLVIANNYVQYFMAWEVMGLCSYLLIGFYFHKDSA
ANASRKAFLTTRVGDVGLFLGIMTLFVTGGTLSFVDLPNAVETGAPLLWLAAPLIFCGTVGKSAQFPLHVWLPDAMEGPT
PVSALIHAATMVAAGVYLVARSFVLFTSFPMVMPWVMGIGTLTAFMAAYIALTATDIKKVLAFSTISQLGYMVTAMGLGG
MTAGTFHLMTHAFFKALLFLGAGSVIHGAHEQDIRQLGGLFSKMKHTGATFVIASLAIAGVPPLSGFWSKDEILLEAYQE
GHFVVYGVLLLTAFMTAFYMFRLVFMTFFGKARNPEIHAHESPALMTVPLWCLAIGAIFVGIPGSPFMDNWFQTFLTGEH
HAAAIDWMVMGSSIAAGVLGIGLAAVLYLAKPQWPVAIANTFRPLYLLSVNQFWIDDIYSVLILNPFQAFGEFLFGFDRL
VIDGLVNSSATAMQKMSKIQLWIDRNIVDGLVNLTGRGTVIGGDILKLFQTGSTQFYLLITCVSVLAFLSVELTSGFQEL
PLLREELYGISSFILDNIFPARWGGHNPFAP

Sequences:

>Translated_671_residues
MTDFAYLIPLFPLIAFAVNLFFGHRLGSFSGWISVLTSVASSAIALPAAWVVAQGGQYSHEWLWLTLGNYDLNFGYLLDP
LSATLLFVVTVIGTLIQVYSIGYMHGEKYYHRFFAYVSLFMAAMLTLVIANNYVQYFMAWEVMGLCSYLLIGFYFHKDSA
ANASRKAFLTTRVGDVGLFLGIMTLFVTGGTLSFVDLPNAVETGAPLLWLAAPLIFCGTVGKSAQFPLHVWLPDAMEGPT
PVSALIHAATMVAAGVYLVARSFVLFTSFPMVMPWVMGIGTLTAFMAAYIALTATDIKKVLAFSTISQLGYMVTAMGLGG
MTAGTFHLMTHAFFKALLFLGAGSVIHGAHEQDIRQLGGLFSKMKHTGATFVIASLAIAGVPPLSGFWSKDEILLEAYQE
GHFVVYGVLLLTAFMTAFYMFRLVFMTFFGKARNPEIHAHESPALMTVPLWCLAIGAIFVGIPGSPFMDNWFQTFLTGEH
HAAAIDWMVMGSSIAAGVLGIGLAAVLYLAKPQWPVAIANTFRPLYLLSVNQFWIDDIYSVLILNPFQAFGEFLFGFDRL
VIDGLVNSSATAMQKMSKIQLWIDRNIVDGLVNLTGRGTVIGGDILKLFQTGSTQFYLLITCVSVLAFLSVELTSGFQEL
PLLREELYGISSFILDNIFPARWGGHNPFAP
>Mature_670_residues
TDFAYLIPLFPLIAFAVNLFFGHRLGSFSGWISVLTSVASSAIALPAAWVVAQGGQYSHEWLWLTLGNYDLNFGYLLDPL
SATLLFVVTVIGTLIQVYSIGYMHGEKYYHRFFAYVSLFMAAMLTLVIANNYVQYFMAWEVMGLCSYLLIGFYFHKDSAA
NASRKAFLTTRVGDVGLFLGIMTLFVTGGTLSFVDLPNAVETGAPLLWLAAPLIFCGTVGKSAQFPLHVWLPDAMEGPTP
VSALIHAATMVAAGVYLVARSFVLFTSFPMVMPWVMGIGTLTAFMAAYIALTATDIKKVLAFSTISQLGYMVTAMGLGGM
TAGTFHLMTHAFFKALLFLGAGSVIHGAHEQDIRQLGGLFSKMKHTGATFVIASLAIAGVPPLSGFWSKDEILLEAYQEG
HFVVYGVLLLTAFMTAFYMFRLVFMTFFGKARNPEIHAHESPALMTVPLWCLAIGAIFVGIPGSPFMDNWFQTFLTGEHH
AAAIDWMVMGSSIAAGVLGIGLAAVLYLAKPQWPVAIANTFRPLYLLSVNQFWIDDIYSVLILNPFQAFGEFLFGFDRLV
IDGLVNSSATAMQKMSKIQLWIDRNIVDGLVNLTGRGTVIGGDILKLFQTGSTQFYLLITCVSVLAFLSVELTSGFQELP
LLREELYGISSFILDNIFPARWGGHNPFAP

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:
GO:0008137: Proton-translocating NAD(P)H-quinone oxidoreductase, chain L
GO:0016491: Proton-translocating NAD(P)H-quinone oxidoreductase, chain L
GO:0042773: Proton-translocating NAD(P)H-quinone oxidoreductase, chain L
GO:0048038: Proton-translocating NAD(P)H-quinone oxidoreductase, chain L
GO:0055114: Proton-translocating NAD(P)H-quinone oxidoreductase, chain L

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=419, Percent_Identity=37.9474940334129, Blast_Score=255, Evalue=8e-68,
Organism=Escherichia coli, GI1788614, Length=561, Percent_Identity=42.7807486631016, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788829, Length=330, Percent_Identity=35.7575757575758, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788827, Length=425, Percent_Identity=28, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788831, Length=395, Percent_Identity=29.1139240506329, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI2367154, Length=430, Percent_Identity=26.5116279069767, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788613, Length=443, Percent_Identity=26.1851015801354, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI145693160, Length=366, Percent_Identity=25.6830601092896, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 73420; Mature: 73289

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFAYLIPLFPLIAFAVNLFFGHRLGSFSGWISVLTSVASSAIALPAAWVVAQGGQYSH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EWLWLTLGNYDLNFGYLLDPLSATLLFVVTVIGTLIQVYSIGYMHGEKYYHRFFAYVSLF
CEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAAMLTLVIANNYVQYFMAWEVMGLCSYLLIGFYFHKDSAANASRKAFLTTRVGDVGLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCHHHHHH
GIMTLFVTGGTLSFVDLPNAVETGAPLLWLAAPLIFCGTVGKSAQFPLHVWLPDAMEGPT
HHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
PVSALIHAATMVAAGVYLVARSFVLFTSFPMVMPWVMGIGTLTAFMAAYIALTATDIKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFSTISQLGYMVTAMGLGGMTAGTFHLMTHAFFKALLFLGAGSVIHGAHEQDIRQLGGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
FSKMKHTGATFVIASLAIAGVPPLSGFWSKDEILLEAYQEGHFVVYGVLLLTAFMTAFYM
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FRLVFMTFFGKARNPEIHAHESPALMTVPLWCLAIGAIFVGIPGSPFMDNWFQTFLTGEH
HHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
HAAAIDWMVMGSSIAAGVLGIGLAAVLYLAKPQWPVAIANTFRPLYLLSVNQFWIDDIYS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCEEEEECHHHHHHHHHH
VLILNPFQAFGEFLFGFDRLVIDGLVNSSATAMQKMSKIQLWIDRNIVDGLVNLTGRGTV
HHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
IGGDILKLFQTGSTQFYLLITCVSVLAFLSVELTSGFQELPLLREELYGISSFILDNIFP
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
ARWGGHNPFAP
CCCCCCCCCCC
>Mature Secondary Structure 
TDFAYLIPLFPLIAFAVNLFFGHRLGSFSGWISVLTSVASSAIALPAAWVVAQGGQYSH
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EWLWLTLGNYDLNFGYLLDPLSATLLFVVTVIGTLIQVYSIGYMHGEKYYHRFFAYVSLF
CEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAAMLTLVIANNYVQYFMAWEVMGLCSYLLIGFYFHKDSAANASRKAFLTTRVGDVGLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCHHHHHH
GIMTLFVTGGTLSFVDLPNAVETGAPLLWLAAPLIFCGTVGKSAQFPLHVWLPDAMEGPT
HHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
PVSALIHAATMVAAGVYLVARSFVLFTSFPMVMPWVMGIGTLTAFMAAYIALTATDIKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFSTISQLGYMVTAMGLGGMTAGTFHLMTHAFFKALLFLGAGSVIHGAHEQDIRQLGGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
FSKMKHTGATFVIASLAIAGVPPLSGFWSKDEILLEAYQEGHFVVYGVLLLTAFMTAFYM
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FRLVFMTFFGKARNPEIHAHESPALMTVPLWCLAIGAIFVGIPGSPFMDNWFQTFLTGEH
HHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
HAAAIDWMVMGSSIAAGVLGIGLAAVLYLAKPQWPVAIANTFRPLYLLSVNQFWIDDIYS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCEEEEECHHHHHHHHHH
VLILNPFQAFGEFLFGFDRLVIDGLVNSSATAMQKMSKIQLWIDRNIVDGLVNLTGRGTV
HHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
IGGDILKLFQTGSTQFYLLITCVSVLAFLSVELTSGFQELPLLREELYGISSFILDNIFP
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
ARWGGHNPFAP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]