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The map label for this gene is mdh [H]

Identifier: 158335293

GI number: 158335293

Start: 2131435

End: 2132421

Strand: Reverse

Name: mdh [H]

Synonym: AM1_2135

Alternate gene names: 158335293

Gene position: 2132421-2131435 (Counterclockwise)

Preceding gene: 158335294

Following gene: 158335284

Centisome position: 32.79

GC content: 51.47

Gene sequence:

>987_bases
TTGTCCGACCACAATAGAGGTATGGACCTCACCTGCTTACCGGGAACCCAAGCCCCCCGCGTCACGATTGTGGGAGCGGG
CAATGTTGGCAGTACCTTAGGACAGCGCATTGTCGAGAAGAATATTGCTGACGTCGTTCTGCTGGATATTCAAGCTGGTC
GCCCTCAAGGATTAGCCCTAGATTTAATGGAAGCACGTGGTGTCGAACACCATGACCGCACCATTATCGGCACAGCAGAT
TATGCCGATACCCAAAACTCGGATATCATCGTCATTACAGCTGGCATCCCCCGCAAACCAGGCATGAGTCGTGACGATCT
GCTCAAGGTGAATGCCCAGATCATTACCGATGTTACTCGCCAAGCAATTGCCCAGTCGCCTAACGCCATTTTGATGGTGG
TTACTAATCCTTTAGACGTGATGACCTATCTGGCTTGGCAGGCCAGTGGATTGTCACCAGAACGCGTCATCGGCATGGCA
GGTGTTTTAGATGCAGCCCGGTTTGAAACCTTTATTGCCCTAGAACTCAAAGTCTCGATTGCCAATGTTCATGCCATGGT
GCTAGGAGGGCATGGTGATCTGATGGTGCCCCTGCCCCGCTATTCCACCGTTAGCGGTATCCCGATCACCGAATTGATGG
ATGAGGAAACTATTCAGCAATTAGTGGATCGCACTCGCAATGGCGGAGCCGAAATTGTTGGCCTCATGCAGGCCGGTAGC
GCCTATTTTGCTCCGGCTTCTTCTGCATCCTTGATGGTGGAATCTATCCTATTTAATCGGTCCCGTATCTTACCGGCTGC
CGCTTACCTGGATGGGCAATATGGTCTCTCCGATATTTTCTTGGGCGTTCCCACCTGTTTAAGTCGCCAAGGTGTAACGA
GGGTATTGGAACTAGACTTATCTGCTGAGGACTATCAGGCATTGCAGACTTCCGCCCAGGCGGTCCGACAGAATATTGCC
ACCACAAAAACTTTACTGACTGCCTAG

Upstream 100 bases:

>100_bases
CAGTTTCGCGTCCCTGTGCCAACGGTTTGGCTCCGGGTTGATCAGCTAGAAGCGGCCTAATCATTTCAACGATTGGTTAC
GGATTAAGTGCGATCGCAAT

Downstream 100 bases:

>100_bases
TTATAGCGACCGTTTAAATAGTTGAGAATTCCCTGGGCAATAGCTTGGGCCATTTGTTTACGCCAGGCTGGATTTCTTAG
CCTAACAATGTCTTGGGCAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSDHNRGMDLTCLPGTQAPRVTIVGAGNVGSTLGQRIVEKNIADVVLLDIQAGRPQGLALDLMEARGVEHHDRTIIGTAD
YADTQNSDIIVITAGIPRKPGMSRDDLLKVNAQIITDVTRQAIAQSPNAILMVVTNPLDVMTYLAWQASGLSPERVIGMA
GVLDAARFETFIALELKVSIANVHAMVLGGHGDLMVPLPRYSTVSGIPITELMDEETIQQLVDRTRNGGAEIVGLMQAGS
AYFAPASSASLMVESILFNRSRILPAAAYLDGQYGLSDIFLGVPTCLSRQGVTRVLELDLSAEDYQALQTSAQAVRQNIA
TTKTLLTA

Sequences:

>Translated_328_residues
MSDHNRGMDLTCLPGTQAPRVTIVGAGNVGSTLGQRIVEKNIADVVLLDIQAGRPQGLALDLMEARGVEHHDRTIIGTAD
YADTQNSDIIVITAGIPRKPGMSRDDLLKVNAQIITDVTRQAIAQSPNAILMVVTNPLDVMTYLAWQASGLSPERVIGMA
GVLDAARFETFIALELKVSIANVHAMVLGGHGDLMVPLPRYSTVSGIPITELMDEETIQQLVDRTRNGGAEIVGLMQAGS
AYFAPASSASLMVESILFNRSRILPAAAYLDGQYGLSDIFLGVPTCLSRQGVTRVLELDLSAEDYQALQTSAQAVRQNIA
TTKTLLTA
>Mature_327_residues
SDHNRGMDLTCLPGTQAPRVTIVGAGNVGSTLGQRIVEKNIADVVLLDIQAGRPQGLALDLMEARGVEHHDRTIIGTADY
ADTQNSDIIVITAGIPRKPGMSRDDLLKVNAQIITDVTRQAIAQSPNAILMVVTNPLDVMTYLAWQASGLSPERVIGMAG
VLDAARFETFIALELKVSIANVHAMVLGGHGDLMVPLPRYSTVSGIPITELMDEETIQQLVDRTRNGGAEIVGLMQAGSA
YFAPASSASLMVESILFNRSRILPAAAYLDGQYGLSDIFLGVPTCLSRQGVTRVLELDLSAEDYQALQTSAQAVRQNIAT
TKTLLTA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:
GO:0003824: Malate dehydrogenase
GO:0005488: Malate dehydrogenase
GO:0005975: Malate dehydrogenase
GO:0006099: Malate dehydrogenase
GO:0006108: Malate dehydrogenase
GO:0016491: Malate dehydrogenase
GO:0016616: Malate dehydrogenase
GO:0030060: Malate dehydrogenase
GO:0044262: Malate dehydrogenase
GO:0055114: Malate dehydrogenase
GO:0005737: Malate dehydrogenase, putative
GO:0008152: Malate dehydrogenase, putative

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=307, Percent_Identity=33.2247557003257, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI221136809, Length=307, Percent_Identity=33.2247557003257, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=32.1192052980132, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=32.1192052980132, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=31.8327974276527, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=31.8327974276527, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=29.1803278688525, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=31.4754098360656, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=31.4754098360656, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI260099725, Length=203, Percent_Identity=34.9753694581281, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI260099727, Length=203, Percent_Identity=34.9753694581281, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI207028494, Length=188, Percent_Identity=31.3829787234043, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI21735621, Length=291, Percent_Identity=29.553264604811, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI103472011, Length=301, Percent_Identity=25.9136212624585, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789632, Length=320, Percent_Identity=28.4375, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=289, Percent_Identity=28.3737024221453, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17554310, Length=325, Percent_Identity=31.6923076923077, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322765, Length=293, Percent_Identity=26.2798634812287, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320125, Length=326, Percent_Identity=27.3006134969325, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=32.013201320132, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=26.2295081967213, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24647881, Length=314, Percent_Identity=30.2547770700637, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24663599, Length=249, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24663595, Length=284, Percent_Identity=28.169014084507, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35021; Mature: 34890

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHNRGMDLTCLPGTQAPRVTIVGAGNVGSTLGQRIVEKNIADVVLLDIQAGRPQGLAL
CCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEE
DLMEARGVEHHDRTIIGTADYADTQNSDIIVITAGIPRKPGMSRDDLLKVNAQIITDVTR
EEHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHEEHHHHHHHHHHH
QAIAQSPNAILMVVTNPLDVMTYLAWQASGLSPERVIGMAGVLDAARFETFIALELKVSI
HHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEE
ANVHAMVLGGHGDLMVPLPRYSTVSGIPITELMDEETIQQLVDRTRNGGAEIVGLMQAGS
EEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCCCEEEEEEECCC
AYFAPASSASLMVESILFNRSRILPAAAYLDGQYGLSDIFLGVPTCLSRQGVTRVLELDL
EEECCCCCHHHHHHHHHHCCHHCCCHHEEECCCCCHHHHHHCCHHHHCCCCCCEEEEECC
SAEDYQALQTSAQAVRQNIATTKTLLTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDHNRGMDLTCLPGTQAPRVTIVGAGNVGSTLGQRIVEKNIADVVLLDIQAGRPQGLAL
CCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEE
DLMEARGVEHHDRTIIGTADYADTQNSDIIVITAGIPRKPGMSRDDLLKVNAQIITDVTR
EEHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHEEHHHHHHHHHHH
QAIAQSPNAILMVVTNPLDVMTYLAWQASGLSPERVIGMAGVLDAARFETFIALELKVSI
HHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEE
ANVHAMVLGGHGDLMVPLPRYSTVSGIPITELMDEETIQQLVDRTRNGGAEIVGLMQAGS
EEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCCCEEEEEEECCC
AYFAPASSASLMVESILFNRSRILPAAAYLDGQYGLSDIFLGVPTCLSRQGVTRVLELDL
EEECCCCCHHHHHHHHHHCCHHCCCHHEEECCCCCHHHHHHCCHHHHCCCCCCEEEEECC
SAEDYQALQTSAQAVRQNIATTKTLLTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA