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The map label for this gene is nthB

Identifier: 158334374

GI number: 158334374

Start: 1174281

End: 1175027

Strand: Reverse

Name: nthB

Synonym: AM1_1195

Alternate gene names: NA

Gene position: 1175027-1174281 (Counterclockwise)

Preceding gene: 158334375

Following gene: 158334373

Centisome position: 18.07

GC content: 51.67

Gene sequence:

>747_bases
ATGAAACTACAGCATAATCTGGGGGGCCTGGAAGGGCTCGATCCCATCAACGTTGAAAAACAGGTGTTTGTGGAACCCTG
GGAAAAGCGCATCTTTGGTATCCACACGGCCATGATGGCCCTGAGCAATCACCTGGATACCTCACTGCCGAACTATGAGA
TTGCCAAAGTTCCCACCCAATTTAAAAGCTTTTGGACCTGGGGGCATCTCCGCAAAGGAGCTGAAGCCATGCATCCCTTT
GAGTATTTTCGGCTGCGGTATTACGAAAAATGGCTGGGGGGGATTTCGGGCTTCTTTGTGGAAGAGGGCTACTTTACCCA
AGAAGAACTCGATACCCGCACCGCTGAATTTTTAGAAGACAGCACGAAAGCTGCTGCTCCCCTTCCCAAAGGTGGCAAAG
CTGGCATCGATACCCAGGTCGATAAATACCTACGAGAAGGGGACTCACCGATGCGGCCAGCCCCGGCCCCACCCAAATTT
AAGGTGGGGGATCGCGTCAAGGTCAAAAACGTTCATCCTGGTGCCCATAGTCGCTTGCCTGGGCAGTTGCGAGATAGAGA
AGCGGTGGTGGTCTTGGTCTATGAAGGGGCCTTCACCTACTTCTTCCCCACTGAAGATGGGATTGGCACGCCCATGCCAG
TGTATAGCCTGCTGTTCCAGCCCCAAGATATTTGGACTGAATCTCTGACAGAGCCCAATTCCGTTTACTACAACGATATT
TTTGAAGTTTATTTGGAAGCCGCCTAG

Upstream 100 bases:

>100_bases
TCTTTATCGGCAGAAATCTAGATGAAGCCGAACTCCGTAACGAGTTCCTAAGCTGTTTGGCCCAGCCCCTTTCCCCAACC
TTGACCGCAGAGGTATACGC

Downstream 100 bases:

>100_bases
ATTATGCCTAGCAATTACCCTGGATTTAAATACGGTGCCGATCGGGAAACCTACAGTGCTGCCAAGGTTAAGGCTCTAGA
ATCCTTGCTGATCGAAAAAG

Product: nitrile hydratase subunit beta

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKLQHNLGGLEGLDPINVEKQVFVEPWEKRIFGIHTAMMALSNHLDTSLPNYEIAKVPTQFKSFWTWGHLRKGAEAMHPF
EYFRLRYYEKWLGGISGFFVEEGYFTQEELDTRTAEFLEDSTKAAAPLPKGGKAGIDTQVDKYLREGDSPMRPAPAPPKF
KVGDRVKVKNVHPGAHSRLPGQLRDREAVVVLVYEGAFTYFFPTEDGIGTPMPVYSLLFQPQDIWTESLTEPNSVYYNDI
FEVYLEAA

Sequences:

>Translated_248_residues
MKLQHNLGGLEGLDPINVEKQVFVEPWEKRIFGIHTAMMALSNHLDTSLPNYEIAKVPTQFKSFWTWGHLRKGAEAMHPF
EYFRLRYYEKWLGGISGFFVEEGYFTQEELDTRTAEFLEDSTKAAAPLPKGGKAGIDTQVDKYLREGDSPMRPAPAPPKF
KVGDRVKVKNVHPGAHSRLPGQLRDREAVVVLVYEGAFTYFFPTEDGIGTPMPVYSLLFQPQDIWTESLTEPNSVYYNDI
FEVYLEAA
>Mature_248_residues
MKLQHNLGGLEGLDPINVEKQVFVEPWEKRIFGIHTAMMALSNHLDTSLPNYEIAKVPTQFKSFWTWGHLRKGAEAMHPF
EYFRLRYYEKWLGGISGFFVEEGYFTQEELDTRTAEFLEDSTKAAAPLPKGGKAGIDTQVDKYLREGDSPMRPAPAPPKF
KVGDRVKVKNVHPGAHSRLPGQLRDREAVVVLVYEGAFTYFFPTEDGIGTPMPVYSLLFQPQDIWTESLTEPNSVYYNDI
FEVYLEAA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:
GO:0003824: Nitrile hydratase, beta subunit
GO:0006807: Nitrile hydratase, beta subunit
GO:0009536: Nitrile hydratase, beta subunit
GO:0015979: Nitrile hydratase, beta subunit
GO:0018822: Nitrile hydratase, beta subunit
GO:0046914: Nitrile hydratase, beta subunit

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 28256; Mature: 28256

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQHNLGGLEGLDPINVEKQVFVEPWEKRIFGIHTAMMALSNHLDTSLPNYEIAKVPTQ
CCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHH
FKSFWTWGHLRKGAEAMHPFEYFRLRYYEKWLGGISGFFVEEGYFTQEELDTRTAEFLED
HHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHH
STKAAAPLPKGGKAGIDTQVDKYLREGDSPMRPAPAPPKFKVGDRVKVKNVHPGAHSRLP
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GQLRDREAVVVLVYEGAFTYFFPTEDGIGTPMPVYSLLFQPQDIWTESLTEPNSVYYNDI
CCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHH
FEVYLEAA
HHHHHCCC
>Mature Secondary Structure
MKLQHNLGGLEGLDPINVEKQVFVEPWEKRIFGIHTAMMALSNHLDTSLPNYEIAKVPTQ
CCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHH
FKSFWTWGHLRKGAEAMHPFEYFRLRYYEKWLGGISGFFVEEGYFTQEELDTRTAEFLED
HHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHH
STKAAAPLPKGGKAGIDTQVDKYLREGDSPMRPAPAPPKFKVGDRVKVKNVHPGAHSRLP
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GQLRDREAVVVLVYEGAFTYFFPTEDGIGTPMPVYSLLFQPQDIWTESLTEPNSVYYNDI
CCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHH
FEVYLEAA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]