Click here to switch to the map view.

The map label for this gene is nthA

Identifier: 158334373

GI number: 158334373

Start: 1173654

End: 1174277

Strand: Reverse

Name: nthA

Synonym: AM1_1194

Alternate gene names: NA

Gene position: 1174277-1173654 (Counterclockwise)

Preceding gene: 158334374

Following gene: 158334372

Centisome position: 18.06

GC content: 52.24

Gene sequence:

>624_bases
ATGCCTAGCAATTACCCTGGATTTAAATACGGTGCCGATCGGGAAACCTACAGTGCTGCCAAGGTTAAGGCTCTAGAATC
CTTGCTGATCGAAAAAGGGATTATCACAGGCAATACTGTCGATCAAATCCTCGGCTACTTTGAAACGGAAATGGGACCGT
TTAACGGGGCCAAACTGGTGGCCAAAGCCTGGGTGGATTCAGAATTCAAACAGCGGTTATTGGCCGATTGTAATGCCGCC
TGCCAAGAACTAGACTTTCCTGCAGGCATGTCCGGGGCAGAAGGAGAGCATATGCGGATTGTTGAGAATACCCCCGAGGT
CCATAACGTTATTGTCTGTACTTTATGTTCTTGCTATCCCTGGCCCACCCTGGGCTTACCCCCCTACTGGTTTAAAGATC
CGACGTTCCGGGCGCGGGTGGTCAGGGAACCCCGTGTGGTCCTCAAAGAATTTGGCGTCACCCTAGATGATTCTGTGGAG
GTGCGGGTCTGGGATAGCAGTGCTCAGATTCGCTGGTGGGTGTTGCCCATGCGTCCAGCGGGGACTGAAGGTATGAACGA
GACCCAATTGGCTGATTTACTGACACCAGAAGCCATGATGGGTGTTGCCACGGTGCAAGTCTAG

Upstream 100 bases:

>100_bases
GCTGTTCCAGCCCCAAGATATTTGGACTGAATCTCTGACAGAGCCCAATTCCGTTTACTACAACGATATTTTTGAAGTTT
ATTTGGAAGCCGCCTAGATT

Downstream 100 bases:

>100_bases
GGGGAGGACAACAGTCATGTTTACCCAGTTTGAGCATTTTGCCGCCACCAGTCTGATGGGGTCGCCCGAAGAAGCTCCTC
CTCGCCAAGACGGTCACCTG

Product: nitrile hydratase subunit alpha

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPSNYPGFKYGADRETYSAAKVKALESLLIEKGIITGNTVDQILGYFETEMGPFNGAKLVAKAWVDSEFKQRLLADCNAA
CQELDFPAGMSGAEGEHMRIVENTPEVHNVIVCTLCSCYPWPTLGLPPYWFKDPTFRARVVREPRVVLKEFGVTLDDSVE
VRVWDSSAQIRWWVLPMRPAGTEGMNETQLADLLTPEAMMGVATVQV

Sequences:

>Translated_207_residues
MPSNYPGFKYGADRETYSAAKVKALESLLIEKGIITGNTVDQILGYFETEMGPFNGAKLVAKAWVDSEFKQRLLADCNAA
CQELDFPAGMSGAEGEHMRIVENTPEVHNVIVCTLCSCYPWPTLGLPPYWFKDPTFRARVVREPRVVLKEFGVTLDDSVE
VRVWDSSAQIRWWVLPMRPAGTEGMNETQLADLLTPEAMMGVATVQV
>Mature_206_residues
PSNYPGFKYGADRETYSAAKVKALESLLIEKGIITGNTVDQILGYFETEMGPFNGAKLVAKAWVDSEFKQRLLADCNAAC
QELDFPAGMSGAEGEHMRIVENTPEVHNVIVCTLCSCYPWPTLGLPPYWFKDPTFRARVVREPRVVLKEFGVTLDDSVEV
RVWDSSAQIRWWVLPMRPAGTEGMNETQLADLLTPEAMMGVATVQV

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23052; Mature: 22920

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNYPGFKYGADRETYSAAKVKALESLLIEKGIITGNTVDQILGYFETEMGPFNGAKLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
AKAWVDSEFKQRLLADCNAACQELDFPAGMSGAEGEHMRIVENTPEVHNVIVCTLCSCYP
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
WPTLGLPPYWFKDPTFRARVVREPRVVLKEFGVTLDDSVEVRVWDSSAQIRWWVLPMRPA
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCC
GTEGMNETQLADLLTPEAMMGVATVQV
CCCCCCHHHHHHHCCHHHHHCEEEECC
>Mature Secondary Structure 
PSNYPGFKYGADRETYSAAKVKALESLLIEKGIITGNTVDQILGYFETEMGPFNGAKLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
AKAWVDSEFKQRLLADCNAACQELDFPAGMSGAEGEHMRIVENTPEVHNVIVCTLCSCYP
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
WPTLGLPPYWFKDPTFRARVVREPRVVLKEFGVTLDDSVEVRVWDSSAQIRWWVLPMRPA
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCC
GTEGMNETQLADLLTPEAMMGVATVQV
CCCCCCHHHHHHHCCHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]