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The map label for this gene is cca [H]

Identifier: 158334195

GI number: 158334195

Start: 979332

End: 982040

Strand: Direct

Name: cca [H]

Synonym: AM1_1013

Alternate gene names: 158334195

Gene position: 979332-982040 (Clockwise)

Preceding gene: 158334194

Following gene: 158334196

Centisome position: 15.06

GC content: 50.31

Gene sequence:

>2709_bases
ATGGATATCGTTTTATGTCATCGCACAGCAGACTTTGACACCTTAGGAGCCGCTGTCGGTATCACTCATTTATATCCGGG
AACTCGAATTGTCCTGTGTGGGGGAGCCCATCCAACGGTTCGGGAGTTTCTAGGGCTCTACAGGGATCAATTTCCTCTAA
TTGAGCATCGGGCGATTAATGCTGAACAGCTTCGCTCAATTACGATCGTCGATGCACAACGGCGCCAGCTTTTAGGACCC
GCAGCAAATTGGCTTGATCTTCCTCAAGTCCAAGTCAAAATTTACGATCACCATCTATCCACAACCGGGAATATTGAAGC
CGATCAGTTGATACTGGAAGCAGTGGGCGCAGCCACCACCCTAGTCGTGGAAGAGTTGCAGCGAAAACACCTGCAGATGC
CTGTGACCGTGGCAACGGTGATGGCTTTGGGAATTCATGTGGATACCGGATCTCTCACTTACCCAAGTTCGACCGTGCGA
GATGCGGCTGCTTTGACTTGGTTAATGACTCAAGGGGCCAATCAACGGGCGATTGCAGAGTACACCGAACCCGTTTTTTC
CTCAGATTTACAGGATTTGCTGACCCAAGCCTTGGAATTAACACAAACCCAGTCTAGACATGGAAATTCTTTGTCCTGGG
TGATGTTGAAGACAGAACGTTATGTGCCTGGTTTGGCCAGCCTGACCTCACAACTGATGCTTCTGCATGAGAGTGACAGT
TTTTTGCTGGGGCATGTCTATAGCAAGGCTGAAACCCAGAAACTAAGTGTGATAGGTCGTTCTCGGGTTGAAGGAGTTGA
CCTGAATGGACTATTGCAGCCCCTAGGAGGTGGGGGACATGCCCGGGCAGCTGCAGTGACGTTAAAAACAGAAACGCCCC
AAAGGGTAATGGAGGACTTAGTGGCAACGCTGCTGGAGCAGATGCCCTTACCGCCTATGGCGGCAGACTTAATGTCTTCC
CCCGTCCGAACCATTCGCCCCACGACCACCATTGATCAAGCCCGGCGAATTTTGTTGCGCTATGGCCATTCTGGGCTGTC
AGTGGTGAATGACCAAGATCAGTTGGTAGGGATTTTATCTCGGCGTGATCTCGATATTGCCCTCCATCATGGCTTTGGAC
ATGCTCCAGTTAAAGGTTATATGACGGCCCCAGTTCGCACGATTAGTTTGGGGACCTCTCTGCCTGAGATTGAATTGATG
ATGGTGACCTACGATATTGGCCGGTTGCCAGTGGTGGAACAGGGGCAATTGGTGGGGATCGTAACCCGTACGGATATCCT
GCGCCAGCTACATCAACTGAAGCATCCAGTACTCCCGACCCATCAACAAGTAGTGCGCACATCCATTCAGGAACAACTGC
GATCGCAACTGTCTCCCACATTGCAAACCCTGTTATCGACGGCGGCAGATGCGGCAGCAGCTCAAGGCTGGCAGCTGTAT
TTAGTGGGGGGAGCGGTACGGGATTTGCTGCTCTCAGGCCCAAACCAGCCCTTTAGGGTGAAAGAGTTTGATCTCGTTGT
GGATGGCGTTGAAACCACTGCAAAAGCGGCAGGGGTTTCTCTCGCCCAAACCTTACAGTCAGACTATCCTGAGACCCAAT
TGCAGGTTTATGGCCAGTTCCAAACCGCGGCTCTGATTTGGCCCTCTGGATCCGAGTTAGCTGGATTTTCGGTGGATATT
GCGACAGCGCGCAGTGAGTTTTACCCTTATCCTGCCGCCAATCCAGAGGTAGAAGCAAGCTCCATTCGTCAAGACCTCTA
TCGGCGAGACTTTACCATCAATGCTTTGGCCGTTCGATTAACACGTCAGGGGTTTCAATCCCATCCCAGTGGCGAGTTAC
TAGATTTCTTCGGAGGCGTAGTGGATCTGCAGGATCGCCAAATCCGAGTTCTGCATCCCAACAGCTTTATTGAAGATCCG
ACTCGAATTTTTCGAGCCGTACGGTTTGCAACTCGACTGGAGTTTGAGATTGGAGAACAGACCGAGGCCTATATTCGCCA
TGCGATCGCAAGTGGTATCTATACACAAGTCCAGGCAGAAAAGGATAAAGCGCCAGCCTTGCAATCCCGCCTCAAAAACG
AACTTAAGTACATTTTCAAAACATCTCATTGGGCAAGAGCGCTCAATTTACTGGCTGACTTAGAGGCATTGCAGTGTATC
CATCCTCAGCTCCAAATCAATCAACACTGGTGGCAACGGGTGCGACTGGCCATCTGTTGGTACAACACATTTGAACCCGC
TCGCTTAGAGGTGCCTGTTTGGTTGTTGATTTTAGAAGTATTGCTGACTGCTTTACCCCCTACAGAGCGCTGTGTAGTGG
CCGCTAACTTACATTTATCAGACTCCACTCAACAGAGACTTCAAAATCTGGATGGGGTATTGCAAGAACTTGAAAAGCAA
CCGGAGCAAGCCTCCCCTAGCCAACTCACAGATTTTTTAAAGTCCCACAACATTTCCGGATTGATTTTAGTCGGCATCTA
TAGCAATGTCAGGATTCGCAAGTGGGTGTGGCAGTACCTATCGGATTGGTCGAGCCGCAAACCATTACTGACAGGTAAAG
ACTTGATTCAGTTAGGGTATCGACCGGGGCGAGAATTTAAATCGATGTTGGCAGCCCTTGGGGCTGCTACCTTAGACGGT
GAACTCAAGGACCGAGATCAAGCACAAGCCTGGGTAAAAGCACGTTTTCCCATCCCTCCTAAAACGTAG

Upstream 100 bases:

>100_bases
GCATTTGAGAGTGCCTACGCCAGATAATCATACCCTAAAAGAGGAGATTTACTTGACTGAAGTCGATGGAGCCGTAGGCG
TTGGGATGTAAATAGGCTGG

Downstream 100 bases:

>100_bases
GGTGAAAATTATGCCTGCTATCCTCCCTTGTGTCTGGTAGAGTCATCATACGAATTGCTGGAAACCTTCATGCCCAAGCT
GCACCTGGGACCCAAATGGC

Product: polyA polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 902; Mature: 902

Protein sequence:

>902_residues
MDIVLCHRTADFDTLGAAVGITHLYPGTRIVLCGGAHPTVREFLGLYRDQFPLIEHRAINAEQLRSITIVDAQRRQLLGP
AANWLDLPQVQVKIYDHHLSTTGNIEADQLILEAVGAATTLVVEELQRKHLQMPVTVATVMALGIHVDTGSLTYPSSTVR
DAAALTWLMTQGANQRAIAEYTEPVFSSDLQDLLTQALELTQTQSRHGNSLSWVMLKTERYVPGLASLTSQLMLLHESDS
FLLGHVYSKAETQKLSVIGRSRVEGVDLNGLLQPLGGGGHARAAAVTLKTETPQRVMEDLVATLLEQMPLPPMAADLMSS
PVRTIRPTTTIDQARRILLRYGHSGLSVVNDQDQLVGILSRRDLDIALHHGFGHAPVKGYMTAPVRTISLGTSLPEIELM
MVTYDIGRLPVVEQGQLVGIVTRTDILRQLHQLKHPVLPTHQQVVRTSIQEQLRSQLSPTLQTLLSTAADAAAAQGWQLY
LVGGAVRDLLLSGPNQPFRVKEFDLVVDGVETTAKAAGVSLAQTLQSDYPETQLQVYGQFQTAALIWPSGSELAGFSVDI
ATARSEFYPYPAANPEVEASSIRQDLYRRDFTINALAVRLTRQGFQSHPSGELLDFFGGVVDLQDRQIRVLHPNSFIEDP
TRIFRAVRFATRLEFEIGEQTEAYIRHAIASGIYTQVQAEKDKAPALQSRLKNELKYIFKTSHWARALNLLADLEALQCI
HPQLQINQHWWQRVRLAICWYNTFEPARLEVPVWLLILEVLLTALPPTERCVVAANLHLSDSTQQRLQNLDGVLQELEKQ
PEQASPSQLTDFLKSHNISGLILVGIYSNVRIRKWVWQYLSDWSSRKPLLTGKDLIQLGYRPGREFKSMLAALGAATLDG
ELKDRDQAQAWVKARFPIPPKT

Sequences:

>Translated_902_residues
MDIVLCHRTADFDTLGAAVGITHLYPGTRIVLCGGAHPTVREFLGLYRDQFPLIEHRAINAEQLRSITIVDAQRRQLLGP
AANWLDLPQVQVKIYDHHLSTTGNIEADQLILEAVGAATTLVVEELQRKHLQMPVTVATVMALGIHVDTGSLTYPSSTVR
DAAALTWLMTQGANQRAIAEYTEPVFSSDLQDLLTQALELTQTQSRHGNSLSWVMLKTERYVPGLASLTSQLMLLHESDS
FLLGHVYSKAETQKLSVIGRSRVEGVDLNGLLQPLGGGGHARAAAVTLKTETPQRVMEDLVATLLEQMPLPPMAADLMSS
PVRTIRPTTTIDQARRILLRYGHSGLSVVNDQDQLVGILSRRDLDIALHHGFGHAPVKGYMTAPVRTISLGTSLPEIELM
MVTYDIGRLPVVEQGQLVGIVTRTDILRQLHQLKHPVLPTHQQVVRTSIQEQLRSQLSPTLQTLLSTAADAAAAQGWQLY
LVGGAVRDLLLSGPNQPFRVKEFDLVVDGVETTAKAAGVSLAQTLQSDYPETQLQVYGQFQTAALIWPSGSELAGFSVDI
ATARSEFYPYPAANPEVEASSIRQDLYRRDFTINALAVRLTRQGFQSHPSGELLDFFGGVVDLQDRQIRVLHPNSFIEDP
TRIFRAVRFATRLEFEIGEQTEAYIRHAIASGIYTQVQAEKDKAPALQSRLKNELKYIFKTSHWARALNLLADLEALQCI
HPQLQINQHWWQRVRLAICWYNTFEPARLEVPVWLLILEVLLTALPPTERCVVAANLHLSDSTQQRLQNLDGVLQELEKQ
PEQASPSQLTDFLKSHNISGLILVGIYSNVRIRKWVWQYLSDWSSRKPLLTGKDLIQLGYRPGREFKSMLAALGAATLDG
ELKDRDQAQAWVKARFPIPPKT
>Mature_902_residues
MDIVLCHRTADFDTLGAAVGITHLYPGTRIVLCGGAHPTVREFLGLYRDQFPLIEHRAINAEQLRSITIVDAQRRQLLGP
AANWLDLPQVQVKIYDHHLSTTGNIEADQLILEAVGAATTLVVEELQRKHLQMPVTVATVMALGIHVDTGSLTYPSSTVR
DAAALTWLMTQGANQRAIAEYTEPVFSSDLQDLLTQALELTQTQSRHGNSLSWVMLKTERYVPGLASLTSQLMLLHESDS
FLLGHVYSKAETQKLSVIGRSRVEGVDLNGLLQPLGGGGHARAAAVTLKTETPQRVMEDLVATLLEQMPLPPMAADLMSS
PVRTIRPTTTIDQARRILLRYGHSGLSVVNDQDQLVGILSRRDLDIALHHGFGHAPVKGYMTAPVRTISLGTSLPEIELM
MVTYDIGRLPVVEQGQLVGIVTRTDILRQLHQLKHPVLPTHQQVVRTSIQEQLRSQLSPTLQTLLSTAADAAAAQGWQLY
LVGGAVRDLLLSGPNQPFRVKEFDLVVDGVETTAKAAGVSLAQTLQSDYPETQLQVYGQFQTAALIWPSGSELAGFSVDI
ATARSEFYPYPAANPEVEASSIRQDLYRRDFTINALAVRLTRQGFQSHPSGELLDFFGGVVDLQDRQIRVLHPNSFIEDP
TRIFRAVRFATRLEFEIGEQTEAYIRHAIASGIYTQVQAEKDKAPALQSRLKNELKYIFKTSHWARALNLLADLEALQCI
HPQLQINQHWWQRVRLAICWYNTFEPARLEVPVWLLILEVLLTALPPTERCVVAANLHLSDSTQQRLQNLDGVLQELEKQ
PEQASPSQLTDFLKSHNISGLILVGIYSNVRIRKWVWQYLSDWSSRKPLLTGKDLIQLGYRPGREFKSMLAALGAATLDG
ELKDRDQAQAWVKARFPIPPKT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=251, Percent_Identity=31.4741035856574, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 100331; Mature: 100331

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVLCHRTADFDTLGAAVGITHLYPGTRIVLCGGAHPTVREFLGLYRDQFPLIEHRAIN
CCEEEEECCCCCHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHCCCC
AEQLRSITIVDAQRRQLLGPAANWLDLPQVQVKIYDHHLSTTGNIEADQLILEAVGAATT
HHHHCEEEEEEHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LVVEELQRKHLQMPVTVATVMALGIHVDTGSLTYPSSTVRDAAALTWLMTQGANQRAIAE
HHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
YTEPVFSSDLQDLLTQALELTQTQSRHGNSLSWVMLKTERYVPGLASLTSQLMLLHESDS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHEEEECCCC
FLLGHVYSKAETQKLSVIGRSRVEGVDLNGLLQPLGGGGHARAAAVTLKTETPQRVMEDL
EEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHH
VATLLEQMPLPPMAADLMSSPVRTIRPTTTIDQARRILLRYGHSGLSVVNDQDQLVGILS
HHHHHHHCCCCCHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHH
RRDLDIALHHGFGHAPVKGYMTAPVRTISLGTSLPEIELMMVTYDIGRLPVVEQGQLVGI
CCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCCEEEEEEEEECCCCCEECCCCEEEE
VTRTDILRQLHQLKHPVLPTHQQVVRTSIQEQLRSQLSPTLQTLLSTAADAAAAQGWQLY
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LVGGAVRDLLLSGPNQPFRVKEFDLVVDGVETTAKAAGVSLAQTLQSDYPETQLQVYGQF
EECHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECE
QTAALIWPSGSELAGFSVDIATARSEFYPYPAANPEVEASSIRQDLYRRDFTINALAVRL
EEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
TRQGFQSHPSGELLDFFGGVVDLQDRQIRVLHPNSFIEDPTRIFRAVRFATRLEFEIGEQ
HHHHHHCCCCCCHHHHHCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCH
TEAYIRHAIASGIYTQVQAEKDKAPALQSRLKNELKYIFKTSHWARALNLLADLEALQCI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPQLQINQHWWQRVRLAICWYNTFEPARLEVPVWLLILEVLLTALPPTERCVVAANLHLS
CCHHEECHHHHHHHEEEEEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCEEEEEEEECC
DSTQQRLQNLDGVLQELEKQPEQASPSQLTDFLKSHNISGLILVGIYSNVRIRKWVWQYL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
SDWSSRKPLLTGKDLIQLGYRPGREFKSMLAALGAATLDGELKDRDQAQAWVKARFPIPP
HHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCC
KT
CC
>Mature Secondary Structure
MDIVLCHRTADFDTLGAAVGITHLYPGTRIVLCGGAHPTVREFLGLYRDQFPLIEHRAIN
CCEEEEECCCCCHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHCCCC
AEQLRSITIVDAQRRQLLGPAANWLDLPQVQVKIYDHHLSTTGNIEADQLILEAVGAATT
HHHHCEEEEEEHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LVVEELQRKHLQMPVTVATVMALGIHVDTGSLTYPSSTVRDAAALTWLMTQGANQRAIAE
HHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
YTEPVFSSDLQDLLTQALELTQTQSRHGNSLSWVMLKTERYVPGLASLTSQLMLLHESDS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHEEEECCCC
FLLGHVYSKAETQKLSVIGRSRVEGVDLNGLLQPLGGGGHARAAAVTLKTETPQRVMEDL
EEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHH
VATLLEQMPLPPMAADLMSSPVRTIRPTTTIDQARRILLRYGHSGLSVVNDQDQLVGILS
HHHHHHHCCCCCHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHH
RRDLDIALHHGFGHAPVKGYMTAPVRTISLGTSLPEIELMMVTYDIGRLPVVEQGQLVGI
CCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCCEEEEEEEEECCCCCEECCCCEEEE
VTRTDILRQLHQLKHPVLPTHQQVVRTSIQEQLRSQLSPTLQTLLSTAADAAAAQGWQLY
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LVGGAVRDLLLSGPNQPFRVKEFDLVVDGVETTAKAAGVSLAQTLQSDYPETQLQVYGQF
EECHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECE
QTAALIWPSGSELAGFSVDIATARSEFYPYPAANPEVEASSIRQDLYRRDFTINALAVRL
EEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
TRQGFQSHPSGELLDFFGGVVDLQDRQIRVLHPNSFIEDPTRIFRAVRFATRLEFEIGEQ
HHHHHHCCCCCCHHHHHCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCH
TEAYIRHAIASGIYTQVQAEKDKAPALQSRLKNELKYIFKTSHWARALNLLADLEALQCI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPQLQINQHWWQRVRLAICWYNTFEPARLEVPVWLLILEVLLTALPPTERCVVAANLHLS
CCHHEECHHHHHHHEEEEEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCEEEEEEEECC
DSTQQRLQNLDGVLQELEKQPEQASPSQLTDFLKSHNISGLILVGIYSNVRIRKWVWQYL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
SDWSSRKPLLTGKDLIQLGYRPGREFKSMLAALGAATLDGELKDRDQAQAWVKARFPIPP
HHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCC
KT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA