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The map label for this gene is pilT [H]

Identifier: 158333617

GI number: 158333617

Start: 391768

End: 392889

Strand: Reverse

Name: pilT [H]

Synonym: AM1_0418

Alternate gene names: 158333617

Gene position: 392889-391768 (Counterclockwise)

Preceding gene: 158333618

Following gene: 158333616

Centisome position: 6.04

GC content: 45.9

Gene sequence:

>1122_bases
ATGGACTACATGATTGAAGATCTGATGGAAGAGGTCGTTGAACGGGGAGGCTCCGATTTACACCTCTCAGTAGGCTTACC
TCCCTACATTCGTATCAACGGAAAATTAACTCCTACTGATTATGATCCGCTCAGTCAAGAGCAATGTCAGCGTCTTATCT
TTAGCATGCTGAATAACACTCAACGTAAGCAGCTAGAGCAAACATGGGAACTAGATTGTTCCTATGGTGTGAGGGGCCTA
GCTCGGTTTAGGGTAAATGTCTATAAAGACCGAGGGACTTATGCTGCGGCATTGCGTGCTCTTAGCTCTAAAATTCCTTC
TATCGAAACATTAGGACTCCCCAATATTGTCCGAGAAATGTCGGAAAAACCACGGGGGTTGGTCCTCGTTACGGGGCCTA
CCGGTTCAGGGAAAACAACCACCCTTGCCTCCATGATTAACAATATCAATCTAACTCGGGCTGAGCATATTCTTACGGTG
GAAGATCCCATTGAATTTGTTTATGAACCCATTAAAAGTTTGATTCATCAACGTCAGGTTGGTGAAGATACCAAAAGCTT
TGCGAATGCGCTACGAGCAGCTCTTCGTGAAGATCCAGATGTTATTCTGGTTGGGGAAATGCGGGATCTCGAAACCATTC
AACTTGCTATCTCTGCAGCAGAAACAGGTCACTTGGTTTTTGGGACACTTCATACCAGTTCAGCTGCTCAAACGGTCGAT
CGAATGGTAGATGTCTTTCCACCTGAGCAACAGACTCAAGTTCGAGTGCAATTGTCTAACTCTTTAGTCGCTGTATTTAG
CCAAACGCTGGTGACACGAAAGAACCCAAAACCAGGCGAGTTTGGGCGAGTCATGGCCCAAGAAATTATGGTATCAACAC
CTGGAATTGCTAACTTAATTCGTGAAGGGAAAACGTCCCAGATCTATTCTGCGATTCAAACTGGTGGAAAGTTGGGGATG
GTAACGTTGGAAAAAGTTTTAGCAGAACTGTATCGTGCAGGTACGATTTCCTTTGAATCTGCTGTATCAAAAACCTCCCG
ACCTGATGAAATGCAACGCCTTATGGGGGCAGCTCCTATGGGGAATCAACGTGGCTCTGGTGCCGCACCCTATCGGCGAT
AA

Upstream 100 bases:

>100_bases
GACACCAAGATTCCAAGATTAATGCAGGAATCCATGAAATATTTTTTGTATTTGCTGAATCAATCAATTTACGCTAGCTG
AGACTATTAAGAGGCAATTT

Downstream 100 bases:

>100_bases
GCATTGAGTATAAACCTAACATTCGTTCTTTCTTCATTTACTGCTGAGAATTGAGTTATGCCAACTTACGTCGTCCGTGC
GCGAGACTCTCAAGGAAAAT

Product: type IV pili twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNTQRKQLEQTWELDCSYGVRGL
ARFRVNVYKDRGTYAAALRALSSKIPSIETLGLPNIVREMSEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTV
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLIREGKTSQIYSAIQTGGKLGM
VTLEKVLAELYRAGTISFESAVSKTSRPDEMQRLMGAAPMGNQRGSGAAPYRR

Sequences:

>Translated_373_residues
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNTQRKQLEQTWELDCSYGVRGL
ARFRVNVYKDRGTYAAALRALSSKIPSIETLGLPNIVREMSEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTV
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLIREGKTSQIYSAIQTGGKLGM
VTLEKVLAELYRAGTISFESAVSKTSRPDEMQRLMGAAPMGNQRGSGAAPYRR
>Mature_373_residues
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNTQRKQLEQTWELDCSYGVRGL
ARFRVNVYKDRGTYAAALRALSSKIPSIETLGLPNIVREMSEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTV
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLIREGKTSQIYSAIQTGGKLGM
VTLEKVLAELYRAGTISFESAVSKTSRPDEMQRLMGAAPMGNQRGSGAAPYRR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=45.4828660436137, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789723, Length=304, Percent_Identity=35.8552631578947, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=35.5871886120996, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41156; Mature: 41156

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNT
CCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCEECCCCCCCCCHHHHHHHHHHHHCCH
QRKQLEQTWELDCSYGVRGLARFRVNVYKDRGTYAAALRALSSKIPSIETLGLPNIVREM
HHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHCCCHHHHHHH
SEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTVEDPIEFVYEPIKSLIHQRQV
HCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHC
GEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
CCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLI
HHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
REGKTSQIYSAIQTGGKLGMVTLEKVLAELYRAGTISFESAVSKTSRPDEMQRLMGAAPM
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCCCC
GNQRGSGAAPYRR
CCCCCCCCCCCCC
>Mature Secondary Structure
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNT
CCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCEECCCCCCCCCHHHHHHHHHHHHCCH
QRKQLEQTWELDCSYGVRGLARFRVNVYKDRGTYAAALRALSSKIPSIETLGLPNIVREM
HHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHCCCHHHHHHH
SEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTVEDPIEFVYEPIKSLIHQRQV
HCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHC
GEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
CCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLI
HHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
REGKTSQIYSAIQTGGKLGMVTLEKVLAELYRAGTISFESAVSKTSRPDEMQRLMGAAPM
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCCCC
GNQRGSGAAPYRR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]