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The map label for this gene is pilB [H]

Identifier: 158333618

GI number: 158333618

Start: 392968

End: 394974

Strand: Reverse

Name: pilB [H]

Synonym: AM1_0419

Alternate gene names: 158333618

Gene position: 394974-392968 (Counterclockwise)

Preceding gene: 158333619

Following gene: 158333617

Centisome position: 6.07

GC content: 44.79

Gene sequence:

>2007_bases
ATGGTTAACTCTTCCTCACAGCGGCGTGCTCTCGTTACTCGGCACAATTTTTCCCCTTTTGGCAATAAGCTGATTTCAAC
AGGTTATGTTGACCAAGACCAAATGCGGGAAGCGACCCTAGAAAGTCGACGCTCAGGACGTTCACTTGTCGAAATTCTTC
AAGGACTCACTGGACAGTCTTTACCACCAGAACTTTTACGTCAATATAAACGACAACAACTTTTTGAACTAAAAATACTG
TATGGCATCGACTCTTTAGATCTTGAGTTAAGCCAAATTCCAACGGATCAAATCGAAGAATTGATCCAGAATTATATTTC
TCTTGACCTCTGTCGACGCTATCAGCTGGTTCCCCTAACCCTTGATAAAGAAACGGATCCAGCAACATTGCTGGTGGCAA
TGGTTGATCCAGATAATTTAGGCGCTCTGGACGATCTAGGGCGTATTCTTAGAGAGAAAAATCTAACCTTGCAACGCATG
GTGATTGCGGTTGAGGACTATCAGCAACTTATTAGTAAGTATCTCGATCAGCAAGCGGCTGAGCAAAAAGCAAATGAAAA
GCCGATCGAGTTCGATTTAGATGATGACTTAGATGCGATCGCAGGTTTAGATGATCTCCCTAGTGACGAACTTGAAACGG
ACTTGGGGGCGGCTCTTCAAGATGCGGAAACAGCTCCCATCATTGGTCTAGTTAACAAAATTCTGGCTAAAGCTTTACAG
AACGGTGTCTCTGATATTCATATTGAACCCCAAGAAGATCACCTTCGTGTTCGATTCCGTAAAGACGGTGTACTCCAACA
GGCCTTTGACCCATTGCCAAGAAAGATCATTCCTGCGGTTATTTCCCGTTTCAAAATCATTTCAGATCTTGATATTGCCG
AGCGCCGCCTTCCGCAAGATGGTCGTATCCGGCGACTTTTTCAAGGGCGCAAAATTGACTTTCGAGTGAATACCTTGCCG
AGCCGATATGGAGAGAAGATTGTACTTCGTATTCTGGATAACTCCTCGACTCAACTGGGATTAGATAAACTTGTTTCAGA
TGAAGATAGTCTAAACATTGTCAGAGAAATGGTCAGTAGACCGTTTGGACTAATTTTGGTGACAGGACCGACTGGTTCTG
GTAAAACAACGACTCTCTATTCTGCCTTAGCCGAATGTAATCACCCAGGCGTCAACATCAGCACTGCTGAGGACCCGATT
GAATATACCCTCCCAGGTTTGACCCAAGTCCAGGTGATTCGAGAAAAAGGAATGGACTTTGCCTCAATTTTGAGAGCGTT
TCTAAGACAAGATCCAGACGTCATTCTAGTGGGTGAGACCCGAGATAAAGAAACGGCTAAAACTGCCATTGAGGCAGCGC
TAACAGGACACCTTGTGCTAACGACTTTGCACACAAATGACGCTGCTAGTGCAGTTGCTCGCCTTGCGGAAATGGAAGTT
GAGCCTTTTATGGTTTCGGCGTCCCTATTGGGTGTTGTTGCCCAGCGTTTGGTTCGGCGAGTATGCTCAGACTGTAAAGT
CCCTTATCAACCTACTCCCACTGAGCTTGGCCGATTTGGGTTATCTGCTTCTCCTGGCACAGAGATTACCTTCTATAAAG
CGAATACTCTCTCAGCTGATGAACTTCAAACCGCTCAAGCTACTGGGCAAACGGTTTGTTCAACTTGTAATGGTATTGGT
TATAAAGGGCGTTGTGGTGTCTATGAAGTCATGCAAATCACTGAAACCCTGCAAACACTAATTACCGAGGGAGCTCCTAC
CGAGCGGATTAAAGAAGTAGCAGTTGAAGAAGGAATGAAAACCCTTCTAGCTTACAGTCTTGAACTAGTTAAGAAAGGGG
ATACTACTCTCGCTGAAGTGGAACGAGTTACCTTTACAGATAGTGGCTTAGAAGCTGAACTTAAGGCTAAGCGCAAAACA
TCTCTAACTTGTCGAGTCTGTGATGCAGATTTAAGACCTGAGTGGTTGGATTGTCCCTATTGCACGACACCAAGATTCCA
AGATTAA

Upstream 100 bases:

>100_bases
TGTGGGCATGCTGAGAACAGCCTCGCCATTGCCTCGACTTGGCAAGGAAGTCTATTTTCAAGCTTACATATCCACGGTTA
GGTCTTTCGCTTTCATCGCT

Downstream 100 bases:

>100_bases
TGCAGGAATCCATGAAATATTTTTTGTATTTGCTGAATCAATCAATTTACGCTAGCTGAGACTATTAAGAGGCAATTTAT
GGACTACATGATTGAAGATC

Product: type IV pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAEQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSADELQTAQATGQTVCSTCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTCRVCDADLRPEWLDCPYCTTPRFQD

Sequences:

>Translated_668_residues
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAEQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSADELQTAQATGQTVCSTCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTCRVCDADLRPEWLDCPYCTTPRFQD
>Mature_668_residues
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAEQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSADELQTAQATGQTVCSTCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTCRVCDADLRPEWLDCPYCTTPRFQD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=490, Percent_Identity=42.2448979591837, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1786296, Length=404, Percent_Identity=40.8415841584158, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI87082188, Length=288, Percent_Identity=35.4166666666667, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 74239; Mature: 74239

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQS
CCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
LPPELLRQYKRQQLFELKILYGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLT
CCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEE
LDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRMVIAVEDYQQLISKYLDQQAA
ECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
HHHCCCCCCEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQD
CCCCEEEECCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC
GRIRRLFQGRKIDFRVNTLPSRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSR
CHHHHHHCCCEEEEEEECCCHHHCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCC
PFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPIEYTLPGLTQVQVIREKGMDF
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCH
ASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHHCC
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSAD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHH
ELQTAQATGQTVCSTCNGIGYKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKTSLTCRVCDADLRPEWLDCPY
HHHHHHHHHHHCCCCHHHHHHHEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCC
CTTPRFQD
CCCCCCCC
>Mature Secondary Structure
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQS
CCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
LPPELLRQYKRQQLFELKILYGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLT
CCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEE
LDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRMVIAVEDYQQLISKYLDQQAA
ECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
HHHCCCCCCEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQD
CCCCEEEECCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC
GRIRRLFQGRKIDFRVNTLPSRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSR
CHHHHHHCCCEEEEEEECCCHHHCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCC
PFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPIEYTLPGLTQVQVIREKGMDF
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCH
ASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHHCC
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSAD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHH
ELQTAQATGQTVCSTCNGIGYKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKTSLTCRVCDADLRPEWLDCPY
HHHHHHHHHHHCCCCHHHHHHHEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCC
CTTPRFQD
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]