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The map label for this gene is uraA [H]

Identifier: 158333245

GI number: 158333245

Start: 11542

End: 12792

Strand: Reverse

Name: uraA [H]

Synonym: AM1_0014

Alternate gene names: 158333245

Gene position: 12792-11542 (Counterclockwise)

Preceding gene: 158333246

Following gene: 158333242

Centisome position: 0.2

GC content: 52.12

Gene sequence:

>1251_bases
ATGAACCTTAGCCCGCCTCATCCGGTTGAATCTCCCAGTTCCTACTCCTTCCGCCTCAAGGACATCATCCTGGGGGCGCA
AATGCTGTTTATCGCCTTTGGGGCGTTGGTCTTAGTTCCTATCCTGGCAGGCTTAGATCCGAATGTGGCTTTATTCACGG
CGGGAGTGGGAACGCTGGTCTTTCAACTGATTACTAAAGGCAAAGTCCCTGTTTTTTTGGCTTCTTCCTTTGCCTTTATT
GCGCCGATTACCGCTGGTATCCAACAATTTGGTTTGGCTGCAACGATGTCCGGGTTGGTCGCGGCTGGATTCTTGTACAT
GCTTTTAAGTATGTTGGTGCTGTGGCGGGGGCCAGACTCAATTTTGAAAGTGTTACCACCGATCGTGACGGGGCCTGTGA
TCATGGTGATTGGCCTATCTTTGGCCCCCATCGCGATTCAAATGGCGTCTGAGGCAGGCTCTAGTTATGGCGTGGGGACG
GCACAGTTCCTAGCTGCTTTGGCCTTAACCACAACGATTGTGGCGGCCCTGTTGGCCCGAGGAACGTTTCAATTAGTCCC
GATTTTGATGGGGATTAGTGTGGGATATGTCGGTGCCTTACTGTTGGGGATTGTGGATTTTTCCCCTGTTGTGGCAGCGC
CTTGGCTAATGCGGCCTCAATTTACTTTACCGACCCTGCATTGGCCTGCGATCGCATTCTTTGTGCCCGTTGCTATTGCC
CCGGCCATTGAGCATATCGGCGATATCCTCGTGATCAGTTCCATCACTGGCAACCCTTTATTGCGTGATCCAGGACTGCA
TCGCACTCTCTTAGGCGATGGTGTGGCCACTTCAATTGCAGCATTGTTGGGAGGCCCTCCCAATACCACCTACTCAGAAG
TTACGGGTGCAGTTGCCCTGACCCGGGCATTTAATCCTGGCATTATGACCTGGGCTGCCCTCATTGCCATTATGCTGTCC
TTCTCCGGTAAATTAGGAGCTTTATTAAGGACCATTCCCCCACCCGTTATGGGAGGGGTGTTGGTGGTGCTGTTCGGCAC
CATTGTCGTAGTGGGAATTAACAGTTTGGTTCAGTCTGGGAGTAACCTGCTGCAGTCCCGCAACATGATTATTGTGGGGG
TGATTCTAGTGTTAGGAGCGGGGAATATGAGCCTAACCATAGGCCAGTTCTCCTTGGAAGGTATTGGGCTGTCCAGCCTA
ATGGGAGTGTTGTTAAATAGCGTTCTCCCGAACCCTAGTCCGGGGGAATAG

Upstream 100 bases:

>100_bases
TTTGAGCCAAGTATGAAGAAAATCCTGGCGCTATCTTGATCCCCAAAAAGGCTGACTGATACTGGAAAAACAGAAATTTC
AAAACCGGTACCGTTTGTTT

Downstream 100 bases:

>100_bases
GGGCTATTATTTAACGGTCGTTTTGGGATCAACTCGGACCGGAATCTCCGCAGTTTTAGAATCTTTGGTACCCAACATCC
GAAGCTCTAGAGACGACCAT

Product: uracil-xanthine permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNLSPPHPVESPSSYSFRLKDIILGAQMLFIAFGALVLVPILAGLDPNVALFTAGVGTLVFQLITKGKVPVFLASSFAFI
APITAGIQQFGLAATMSGLVAAGFLYMLLSMLVLWRGPDSILKVLPPIVTGPVIMVIGLSLAPIAIQMASEAGSSYGVGT
AQFLAALALTTTIVAALLARGTFQLVPILMGISVGYVGALLLGIVDFSPVVAAPWLMRPQFTLPTLHWPAIAFFVPVAIA
PAIEHIGDILVISSITGNPLLRDPGLHRTLLGDGVATSIAALLGGPPNTTYSEVTGAVALTRAFNPGIMTWAALIAIMLS
FSGKLGALLRTIPPPVMGGVLVVLFGTIVVVGINSLVQSGSNLLQSRNMIIVGVILVLGAGNMSLTIGQFSLEGIGLSSL
MGVLLNSVLPNPSPGE

Sequences:

>Translated_416_residues
MNLSPPHPVESPSSYSFRLKDIILGAQMLFIAFGALVLVPILAGLDPNVALFTAGVGTLVFQLITKGKVPVFLASSFAFI
APITAGIQQFGLAATMSGLVAAGFLYMLLSMLVLWRGPDSILKVLPPIVTGPVIMVIGLSLAPIAIQMASEAGSSYGVGT
AQFLAALALTTTIVAALLARGTFQLVPILMGISVGYVGALLLGIVDFSPVVAAPWLMRPQFTLPTLHWPAIAFFVPVAIA
PAIEHIGDILVISSITGNPLLRDPGLHRTLLGDGVATSIAALLGGPPNTTYSEVTGAVALTRAFNPGIMTWAALIAIMLS
FSGKLGALLRTIPPPVMGGVLVVLFGTIVVVGINSLVQSGSNLLQSRNMIIVGVILVLGAGNMSLTIGQFSLEGIGLSSL
MGVLLNSVLPNPSPGE
>Mature_416_residues
MNLSPPHPVESPSSYSFRLKDIILGAQMLFIAFGALVLVPILAGLDPNVALFTAGVGTLVFQLITKGKVPVFLASSFAFI
APITAGIQQFGLAATMSGLVAAGFLYMLLSMLVLWRGPDSILKVLPPIVTGPVIMVIGLSLAPIAIQMASEAGSSYGVGT
AQFLAALALTTTIVAALLARGTFQLVPILMGISVGYVGALLLGIVDFSPVVAAPWLMRPQFTLPTLHWPAIAFFVPVAIA
PAIEHIGDILVISSITGNPLLRDPGLHRTLLGDGVATSIAALLGGPPNTTYSEVTGAVALTRAFNPGIMTWAALIAIMLS
FSGKLGALLRTIPPPVMGGVLVVLFGTIVVVGINSLVQSGSNLLQSRNMIIVGVILVLGAGNMSLTIGQFSLEGIGLSSL
MGVLLNSVLPNPSPGE

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=458, Percent_Identity=24.235807860262, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI44680143, Length=462, Percent_Identity=24.025974025974, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI44680148, Length=438, Percent_Identity=23.0593607305936, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316845, Length=438, Percent_Identity=23.0593607305936, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788843, Length=387, Percent_Identity=37.4677002583979, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87081818, Length=409, Percent_Identity=37.1638141809291, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082181, Length=384, Percent_Identity=28.90625, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082178, Length=269, Percent_Identity=26.3940520446097, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790087, Length=395, Percent_Identity=24.3037974683544, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=217, Percent_Identity=28.110599078341, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17542260, Length=217, Percent_Identity=27.6497695852535, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356175, Length=287, Percent_Identity=26.4808362369338, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 42840; Mature: 42840

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSPPHPVESPSSYSFRLKDIILGAQMLFIAFGALVLVPILAGLDPNVALFTAGVGTLV
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHH
FQLITKGKVPVFLASSFAFIAPITAGIQQFGLAATMSGLVAAGFLYMLLSMLVLWRGPDS
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ILKVLPPIVTGPVIMVIGLSLAPIAIQMASEAGSSYGVGTAQFLAALALTTTIVAALLAR
HHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GTFQLVPILMGISVGYVGALLLGIVDFSPVVAAPWLMRPQFTLPTLHWPAIAFFVPVAIA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
PAIEHIGDILVISSITGNPLLRDPGLHRTLLGDGVATSIAALLGGPPNTTYSEVTGAVAL
HHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
TRAFNPGIMTWAALIAIMLSFSGKLGALLRTIPPPVMGGVLVVLFGTIVVVGINSLVQSG
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLLQSRNMIIVGVILVLGAGNMSLTIGQFSLEGIGLSSLMGVLLNSVLPNPSPGE
HHHHHHCCHHHHHHHHHHCCCCCEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLSPPHPVESPSSYSFRLKDIILGAQMLFIAFGALVLVPILAGLDPNVALFTAGVGTLV
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHH
FQLITKGKVPVFLASSFAFIAPITAGIQQFGLAATMSGLVAAGFLYMLLSMLVLWRGPDS
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ILKVLPPIVTGPVIMVIGLSLAPIAIQMASEAGSSYGVGTAQFLAALALTTTIVAALLAR
HHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GTFQLVPILMGISVGYVGALLLGIVDFSPVVAAPWLMRPQFTLPTLHWPAIAFFVPVAIA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
PAIEHIGDILVISSITGNPLLRDPGLHRTLLGDGVATSIAALLGGPPNTTYSEVTGAVAL
HHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
TRAFNPGIMTWAALIAIMLSFSGKLGALLRTIPPPVMGGVLVVLFGTIVVVGINSLVQSG
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLLQSRNMIIVGVILVLGAGNMSLTIGQFSLEGIGLSSLMGVLLNSVLPNPSPGE
HHHHHHCCHHHHHHHHHHCCCCCEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]