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The map label for this gene is sppA [C]

Identifier: 158333242

GI number: 158333242

Start: 9275

End: 10096

Strand: Reverse

Name: sppA [C]

Synonym: AM1_0011

Alternate gene names: 158333242

Gene position: 10096-9275 (Counterclockwise)

Preceding gene: 158333245

Following gene: 158333241

Centisome position: 0.16

GC content: 48.3

Gene sequence:

>822_bases
ATGATTTGGCCGTTTAAAGGTGCCCGCAAACAAATAGCCCGTTTGGAGATCAAAGGTGCGATCGCAGGCGCAACCCGAAA
GCATGTCTTGGAAGCCCTCAAAACGATTGAAGAGAAAGGCTATCCAGTGCTTCTCCTCCGTATCGATAGTCCAGGAGGAA
CGGTCGTTGACTCCCATGAAATTTACAGTGCTCTGATCAAATTGCGAGAAAAAATCAAGATTGTTGCCAGCTTTGGCAAT
ATTTCGGCTTCTGGTGGGGTCTATATTGGCATGGGGGCTGAACATATCATGGCTAACCCAGGTACCATCACCGGCAGTAT
TGGCGTGATCCTTCGAGGGAATAATCTGGAACGTCTATTAGATAAGGTGGGGGTTTCCTTTAAGGTCATCAAGTCTGGTC
CCTACAAAGACATTCTCGCCTTTGATCGAGAGTTAACTGAACCGGAGCGTCAAATCCTCCAAGATCTGATTGACAGCAGC
TATTCTCAGTTTGTGGAGATTGTGGCAGAAGGTCGAAACCTAGAAGTAGACGCCGTTAAGCAATTTGCTGATGGGCGTGT
CTTCACGGGACAACAAGCGCTTGAACTTGGCCTAGTGGATCGATTAGGAACAGAAGAAGATGCCCGGTGCTGGGCGGCTG
AACTAGCAGGCCTAGATCCTGAGAAGGCCAAATGCAACACAATAGAAGAACATAAATCCTTTTGGAGTAAGCTGCTACCC
GGTAGCCGCCAATCTTCCTGGTCTGTACCTCCCCAAGTTGCGGATTGGGTGAATTTTGAGATAGCAACGAATGGCTTACC
CTTGTGGCTCTATCGACCTTAA

Upstream 100 bases:

>100_bases
TCACAATGGGCTGGTCCTGGAGTGTTACGCTTGGGTCGATCATTTTTTAATCCATTCCCAGATTGATAGTGTTGAAAACA
GTCAGTAATAAATATTGCCC

Downstream 100 bases:

>100_bases
ATATCATGCTTCTCAAGGGTTTAGAGCTAAATTGACATTGCATCCAATTTAGCTTTAATGAGCCTTACATGATTCAATAA
ACAGTTAGTATGGAAAATCG

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIWPFKGARKQIARLEIKGAIAGATRKHVLEALKTIEEKGYPVLLLRIDSPGGTVVDSHEIYSALIKLREKIKIVASFGN
ISASGGVYIGMGAEHIMANPGTITGSIGVILRGNNLERLLDKVGVSFKVIKSGPYKDILAFDRELTEPERQILQDLIDSS
YSQFVEIVAEGRNLEVDAVKQFADGRVFTGQQALELGLVDRLGTEEDARCWAAELAGLDPEKAKCNTIEEHKSFWSKLLP
GSRQSSWSVPPQVADWVNFEIATNGLPLWLYRP

Sequences:

>Translated_273_residues
MIWPFKGARKQIARLEIKGAIAGATRKHVLEALKTIEEKGYPVLLLRIDSPGGTVVDSHEIYSALIKLREKIKIVASFGN
ISASGGVYIGMGAEHIMANPGTITGSIGVILRGNNLERLLDKVGVSFKVIKSGPYKDILAFDRELTEPERQILQDLIDSS
YSQFVEIVAEGRNLEVDAVKQFADGRVFTGQQALELGLVDRLGTEEDARCWAAELAGLDPEKAKCNTIEEHKSFWSKLLP
GSRQSSWSVPPQVADWVNFEIATNGLPLWLYRP
>Mature_273_residues
MIWPFKGARKQIARLEIKGAIAGATRKHVLEALKTIEEKGYPVLLLRIDSPGGTVVDSHEIYSALIKLREKIKIVASFGN
ISASGGVYIGMGAEHIMANPGTITGSIGVILRGNNLERLLDKVGVSFKVIKSGPYKDILAFDRELTEPERQILQDLIDSS
YSQFVEIVAEGRNLEVDAVKQFADGRVFTGQQALELGLVDRLGTEEDARCWAAELAGLDPEKAKCNTIEEHKSFWSKLLP
GSRQSSWSVPPQVADWVNFEIATNGLPLWLYRP

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:
GO:0006508: Signal peptide peptidase SppA, 36K type
GO:0008233: Signal peptide peptidase SppA, 36K type
GO:0000287: Inorganic pyrophosphatase
GO:0004427: Inorganic pyrophosphatase
GO:0005737: Inorganic pyrophosphatase
GO:0006796: Inorganic pyrophosphatase
GO:0016787: Inorganic pyrophosphatase
GO:0046872: Inorganic pyrophosphatase

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=175, Percent_Identity=40, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787527, Length=166, Percent_Identity=32.5301204819277, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 30100; Mature: 30100

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWPFKGARKQIARLEIKGAIAGATRKHVLEALKTIEEKGYPVLLLRIDSPGGTVVDSHE
CCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHH
IYSALIKLREKIKIVASFGNISASGGVYIGMGAEHIMANPGTITGSIGVILRGNNLERLL
HHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCHHHCCCCCEEEECEEEEEECCCHHHHH
DKVGVSFKVIKSGPYKDILAFDRELTEPERQILQDLIDSSYSQFVEIVAEGRNLEVDAVK
HHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QFADGRVFTGQQALELGLVDRLGTEEDARCWAAELAGLDPEKAKCNTIEEHKSFWSKLLP
HHHCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
GSRQSSWSVPPQVADWVNFEIATNGLPLWLYRP
CCCCCCCCCCCHHHHHEEEEEECCCEEEEEECC
>Mature Secondary Structure
MIWPFKGARKQIARLEIKGAIAGATRKHVLEALKTIEEKGYPVLLLRIDSPGGTVVDSHE
CCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHH
IYSALIKLREKIKIVASFGNISASGGVYIGMGAEHIMANPGTITGSIGVILRGNNLERLL
HHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCHHHCCCCCEEEECEEEEEECCCHHHHH
DKVGVSFKVIKSGPYKDILAFDRELTEPERQILQDLIDSSYSQFVEIVAEGRNLEVDAVK
HHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QFADGRVFTGQQALELGLVDRLGTEEDARCWAAELAGLDPEKAKCNTIEEHKSFWSKLLP
HHHCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
GSRQSSWSVPPQVADWVNFEIATNGLPLWLYRP
CCCCCCCCCCCHHHHHEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]