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The map label for this gene is ogt [H]

Identifier: 158318154

GI number: 158318154

Start: 7795140

End: 7795712

Strand: Reverse

Name: ogt [H]

Synonym: Franean1_6419

Alternate gene names: 158318154

Gene position: 7795712-7795140 (Counterclockwise)

Preceding gene: 158318155

Following gene: 158318150

Centisome position: 86.79

GC content: 73.47

Gene sequence:

>573_bases
GTGACCCGCAGCCAACCCGTCCCGCCGGACGCCGCCGAGCGCACGCACGCCGTCGTGGAGAGCCCGGTCGGGCCGCTGAC
GCTGGTGGCGAGCGGCGGCGCGCTCGTCGGCTGCTACCTGGACGCCCAGCGCCACCTGCCGGACCAGGCCTGGTTCGGCG
AATCGGCCCCGGCCGCCCCCGGGACCACCACCTCCGGAGCAGCCGCTCCCGGAGGCGCCGTCCTCCAGACCGCCGCCGGC
CAGCTCGCCGAGTACTTCGCGGGCCGCCGGACGTCCTTCGACCTGCCGCTGAACCCGATCGGCACCGACTTCCAACGCAA
GGTGTGGAAGGCACTGTGCGACATCCCGTACGCGGAGACGATTTCGTACGGCGAGCTGGCGCGCCGCCTCGGCCAGCCGG
GTGCGTCACGCGCGGTGGGACTGGCCAACGGTCGCAACCCGATCAGCATCATCGTCCCGTGCCACCGGGTGATCGGCGCG
AACGGCAGCCTCACCGGCTATGGCGGCGGCCTGGAGCGCAAGCGCCACCTGCTCGCCTTCGAGCAGCAGGCCGCCGGCCT
GTCACTGCTTTGA

Upstream 100 bases:

>100_bases
ATGCCGTCCAGCACCTGTGGGCGGTGCTCGACCATCCAGTCAACCGGATGCCCGCGCCGGATCATCCCGGCCCGGTCACC
GCCCGCCGAGAGGAACGCCT

Downstream 100 bases:

>100_bases
CGCCGTTCCGCAGCGCTGTTCCGTGATGCTGTTCCGCCGATGAGGTGCTCCCCGTGCCCTCAGCGTGGCCTTCCGCGGCG
GCGTTTCTCCCGCCCCACGA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTRSQPVPPDAAERTHAVVESPVGPLTLVASGGALVGCYLDAQRHLPDQAWFGESAPAAPGTTTSGAAAPGGAVLQTAAG
QLAEYFAGRRTSFDLPLNPIGTDFQRKVWKALCDIPYAETISYGELARRLGQPGASRAVGLANGRNPISIIVPCHRVIGA
NGSLTGYGGGLERKRHLLAFEQQAAGLSLL

Sequences:

>Translated_190_residues
MTRSQPVPPDAAERTHAVVESPVGPLTLVASGGALVGCYLDAQRHLPDQAWFGESAPAAPGTTTSGAAAPGGAVLQTAAG
QLAEYFAGRRTSFDLPLNPIGTDFQRKVWKALCDIPYAETISYGELARRLGQPGASRAVGLANGRNPISIIVPCHRVIGA
NGSLTGYGGGLERKRHLLAFEQQAAGLSLL
>Mature_189_residues
TRSQPVPPDAAERTHAVVESPVGPLTLVASGGALVGCYLDAQRHLPDQAWFGESAPAAPGTTTSGAAAPGGAVLQTAAGQ
LAEYFAGRRTSFDLPLNPIGTDFQRKVWKALCDIPYAETISYGELARRLGQPGASRAVGLANGRNPISIIVPCHRVIGAN
GSLTGYGGGLERKRHLLAFEQQAAGLSLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=179, Percent_Identity=32.9608938547486, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1787596, Length=173, Percent_Identity=43.3526011560694, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1788542, Length=113, Percent_Identity=43.3628318584071, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=45.5555555555556, Blast_Score=95, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137554, Length=108, Percent_Identity=39.8148148148148, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19712; Mature: 19580

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSQPVPPDAAERTHAVVESPVGPLTLVASGGALVGCYLDAQRHLPDQAWFGESAPAAP
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEEEEECHHHCCCCHHHCCCCCCCCC
GTTTSGAAAPGGAVLQTAAGQLAEYFAGRRTSFDLPLNPIGTDFQRKVWKALCDIPYAET
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
ISYGELARRLGQPGASRAVGLANGRNPISIIVPCHRVIGANGSLTGYGGGLERKRHLLAF
CCHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHH
EQQAAGLSLL
HHHCCCCCCC
>Mature Secondary Structure 
TRSQPVPPDAAERTHAVVESPVGPLTLVASGGALVGCYLDAQRHLPDQAWFGESAPAAP
CCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEEEEECHHHCCCCHHHCCCCCCCCC
GTTTSGAAAPGGAVLQTAAGQLAEYFAGRRTSFDLPLNPIGTDFQRKVWKALCDIPYAET
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
ISYGELARRLGQPGASRAVGLANGRNPISIIVPCHRVIGANGSLTGYGGGLERKRHLLAF
CCHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHH
EQQAAGLSLL
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]