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The map label for this gene is pur8 [H]

Identifier: 158317950

GI number: 158317950

Start: 7544422

End: 7545948

Strand: Reverse

Name: pur8 [H]

Synonym: Franean1_6210

Alternate gene names: 158317950

Gene position: 7545948-7544422 (Counterclockwise)

Preceding gene: 158317951

Following gene: 158317949

Centisome position: 84.01

GC content: 67.78

Gene sequence:

>1527_bases
ATGTCTGCTGACAGCGCGACCGCCGCCCCCGTGCGACCGGGCACCTCCGCGGCCCCGCCCACGAGCCATCACCTCGGCCT
GGCTCTGTTCGTGATCGCCGCAGCACAGCTGGTGGTGGTCCTCGACGCCACGATCACCAACATCGCTCTACCCAGCATCC
AGACCGATCTCGACGTGTCCGACGCGAACCTGGCCTGGATCGTCAACTCCTACGCGCTGGCCTTCGGTGGGCTGCTACTG
CTCGGCGGACGCACCGGCGATCTCTTCGGCCGGCGACGGATGTTTCAGACCGGCATCGCGATCTTTACGCTGGCCTCACT
GCTGGGAGGACTGGCCCCCAACGAGGCCCTGTTGATCAGCGCCCGCGTCCTGCAAGGCATCGGCGCCGCGCTCGCTGCCC
CCAACGCGCTGGCCCTGATCACTACGACATTTCCACCCGGCAAGCCCCGCAATACGGCTATGGGTGTGTACGCCGCGATG
GGAGGCGTCGGCGCCACAGTCGGTCTGCTGTTGGGCGGCACACTCACCGACGTCCTGAGCTGGCGATGGGTCTTCTTCAT
CAACGTTCCCATCGGCCTGGTCGTCCTCGTCGGAACCCGGAGCCTCGTCGAGGCCGAACGCGACCCGGGCCGCGTGGACG
TCCCCGGCGCTGTCACGGGAACGGGCGGCCTGATCGCACTGGTCTACGGCATCACCCACGGCGGCGAGCACGGCTGGAGC
GACAGCGCCACCCTGACCGCCTTCGCCGTGGCGGCCGTGCTATTGGTGGCGTTCCTTCTCATCCAGGCCCGGACCGCCCA
CCCGATGATGCCGCTACGGCTGTTCCAGGACCGCAACCGGTCTGGTTCCTACGCCACGATGCTGCTCATCGGTGCCGGCA
TGTTCGCGACCTTCTACTTCCTGACGCTGTACATGCAGCTCATCCTCGGCTACAGCCCCGTCCGGACCGGCTTCGCCTAC
CTGCCCTTCAGCTTCGGCATCGCCATCGCCGCCGGTGTCAGCTCCAAACTGGTCACCCACGTCGCCCCACGCGTGATCGC
CGGACCGGGCCTGCTCGCGGCGGCCGCGGGCATGCTCTGGTTCGCCGCCCTCGATCCGGACTCCCCGTACGCCGGACACC
TGATGCCGGCGATGTTCCTCACCGGCCTCGGCCTCGGCCTGAGCTTCGTCCCCATGACACTCGGCGCAGTCAGCGACGTT
GCACACGAGGACTCCGGGATCGCCTCAGCACTCCTGAACACCGCCCAGCAGATCGGCGGAGCCCTCGGCCTCGCGGTCCT
GTCAACCATCGCCACCTCCACGGCCGACAACAAGCTTCCCGAAGCCGCCGGAGCCCTCTACCAAGGCCTGGCCACGAACG
ACGTCATCCTGATCGCGAGAGCACAGGACGCACTGACCCACGGCTACTCCATGGCTTTCGCCGCCGCCGCCGCGATGTTC
CTCGCCGGTCTGATCGTCACCGCCACCGCCATCAACACTGCCAGGCAACACCACATCGAGGACGCGCCTCCCGCACACAT
CGGCTGA

Upstream 100 bases:

>100_bases
TTCACCTCGCCGGAAGCCGCCGATCTAGAACAAGAAGTCTACCCCTGCACCTGTTTTATGGCCGGGGCAATTTTGCCAGC
GCACCTTCTGGAGTACCTGT

Downstream 100 bases:

>100_bases
CCTGGCCGGCGGAGTGGAGTGGCCACTCGCTGTGATGGTTGGCCATTAGATGGCCAATGGCCGCGATGGTTGGCCAGTCC
TCGGGTGGCGTGGCTCGTAT

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSADSATAAPVRPGTSAAPPTSHHLGLALFVIAAAQLVVVLDATITNIALPSIQTDLDVSDANLAWIVNSYALAFGGLLL
LGGRTGDLFGRRRMFQTGIAIFTLASLLGGLAPNEALLISARVLQGIGAALAAPNALALITTTFPPGKPRNTAMGVYAAM
GGVGATVGLLLGGTLTDVLSWRWVFFINVPIGLVVLVGTRSLVEAERDPGRVDVPGAVTGTGGLIALVYGITHGGEHGWS
DSATLTAFAVAAVLLVAFLLIQARTAHPMMPLRLFQDRNRSGSYATMLLIGAGMFATFYFLTLYMQLILGYSPVRTGFAY
LPFSFGIAIAAGVSSKLVTHVAPRVIAGPGLLAAAAGMLWFAALDPDSPYAGHLMPAMFLTGLGLGLSFVPMTLGAVSDV
AHEDSGIASALLNTAQQIGGALGLAVLSTIATSTADNKLPEAAGALYQGLATNDVILIARAQDALTHGYSMAFAAAAAMF
LAGLIVTATAINTARQHHIEDAPPAHIG

Sequences:

>Translated_508_residues
MSADSATAAPVRPGTSAAPPTSHHLGLALFVIAAAQLVVVLDATITNIALPSIQTDLDVSDANLAWIVNSYALAFGGLLL
LGGRTGDLFGRRRMFQTGIAIFTLASLLGGLAPNEALLISARVLQGIGAALAAPNALALITTTFPPGKPRNTAMGVYAAM
GGVGATVGLLLGGTLTDVLSWRWVFFINVPIGLVVLVGTRSLVEAERDPGRVDVPGAVTGTGGLIALVYGITHGGEHGWS
DSATLTAFAVAAVLLVAFLLIQARTAHPMMPLRLFQDRNRSGSYATMLLIGAGMFATFYFLTLYMQLILGYSPVRTGFAY
LPFSFGIAIAAGVSSKLVTHVAPRVIAGPGLLAAAAGMLWFAALDPDSPYAGHLMPAMFLTGLGLGLSFVPMTLGAVSDV
AHEDSGIASALLNTAQQIGGALGLAVLSTIATSTADNKLPEAAGALYQGLATNDVILIARAQDALTHGYSMAFAAAAAMF
LAGLIVTATAINTARQHHIEDAPPAHIG
>Mature_507_residues
SADSATAAPVRPGTSAAPPTSHHLGLALFVIAAAQLVVVLDATITNIALPSIQTDLDVSDANLAWIVNSYALAFGGLLLL
GGRTGDLFGRRRMFQTGIAIFTLASLLGGLAPNEALLISARVLQGIGAALAAPNALALITTTFPPGKPRNTAMGVYAAMG
GVGATVGLLLGGTLTDVLSWRWVFFINVPIGLVVLVGTRSLVEAERDPGRVDVPGAVTGTGGLIALVYGITHGGEHGWSD
SATLTAFAVAAVLLVAFLLIQARTAHPMMPLRLFQDRNRSGSYATMLLIGAGMFATFYFLTLYMQLILGYSPVRTGFAYL
PFSFGIAIAAGVSSKLVTHVAPRVIAGPGLLAAAAGMLWFAALDPDSPYAGHLMPAMFLTGLGLGLSFVPMTLGAVSDVA
HEDSGIASALLNTAQQIGGALGLAVLSTIATSTADNKLPEAAGALYQGLATNDVILIARAQDALTHGYSMAFAAAAAMFL
AGLIVTATAINTARQHHIEDAPPAHIG

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=31.5270935960591, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=25.7075471698113, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=27.007299270073, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=30.1932367149758, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=28.7469287469287, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324954, Length=389, Percent_Identity=26.2210796915167, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323520, Length=444, Percent_Identity=26.8018018018018, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323935, Length=462, Percent_Identity=24.8917748917749, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322958, Length=499, Percent_Identity=24.0480961923848, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=254, Percent_Identity=25.9842519685039, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=23.3160621761658, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=30.0751879699248, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52028; Mature: 51897

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADSATAAPVRPGTSAAPPTSHHLGLALFVIAAAQLVVVLDATITNIALPSIQTDLDVS
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DANLAWIVNSYALAFGGLLLLGGRTGDLFGRRRMFQTGIAIFTLASLLGGLAPNEALLIS
CCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARVLQGIGAALAAPNALALITTTFPPGKPRNTAMGVYAAMGGVGATVGLLLGGTLTDVLS
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WRWVFFINVPIGLVVLVGTRSLVEAERDPGRVDVPGAVTGTGGLIALVYGITHGGEHGWS
CEEEEEEECCHHEEEEECCHHHHHHCCCCCCEECCCCEECCCHHHHHHHHHCCCCCCCCC
DSATLTAFAVAAVLLVAFLLIQARTAHPMMPLRLFQDRNRSGSYATMLLIGAGMFATFYF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LTLYMQLILGYSPVRTGFAYLPFSFGIAIAAGVSSKLVTHVAPRVIAGPGLLAAAAGMLW
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCHHHCCCCHHHHHHHHHE
FAALDPDSPYAGHLMPAMFLTGLGLGLSFVPMTLGAVSDVAHEDSGIASALLNTAQQIGG
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGLAVLSTIATSTADNKLPEAAGALYQGLATNDVILIARAQDALTHGYSMAFAAAAAMF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LAGLIVTATAINTARQHHIEDAPPAHIG
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SADSATAAPVRPGTSAAPPTSHHLGLALFVIAAAQLVVVLDATITNIALPSIQTDLDVS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DANLAWIVNSYALAFGGLLLLGGRTGDLFGRRRMFQTGIAIFTLASLLGGLAPNEALLIS
CCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARVLQGIGAALAAPNALALITTTFPPGKPRNTAMGVYAAMGGVGATVGLLLGGTLTDVLS
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WRWVFFINVPIGLVVLVGTRSLVEAERDPGRVDVPGAVTGTGGLIALVYGITHGGEHGWS
CEEEEEEECCHHEEEEECCHHHHHHCCCCCCEECCCCEECCCHHHHHHHHHCCCCCCCCC
DSATLTAFAVAAVLLVAFLLIQARTAHPMMPLRLFQDRNRSGSYATMLLIGAGMFATFYF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LTLYMQLILGYSPVRTGFAYLPFSFGIAIAAGVSSKLVTHVAPRVIAGPGLLAAAAGMLW
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCHHHCCCCHHHHHHHHHE
FAALDPDSPYAGHLMPAMFLTGLGLGLSFVPMTLGAVSDVAHEDSGIASALLNTAQQIGG
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGLAVLSTIATSTADNKLPEAAGALYQGLATNDVILIARAQDALTHGYSMAFAAAAAMF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LAGLIVTATAINTARQHHIEDAPPAHIG
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]