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The map label for this gene is stp [H]

Identifier: 158316938

GI number: 158316938

Start: 6219749

End: 6221215

Strand: Direct

Name: stp [H]

Synonym: Franean1_5182

Alternate gene names: 158316938

Gene position: 6219749-6221215 (Clockwise)

Preceding gene: 158316937

Following gene: 158316947

Centisome position: 69.25

GC content: 74.57

Gene sequence:

>1467_bases
GTGACCGACAAGCGATCCGCCACTGTCTGGACGTTCGTCGTGACGTCCATAACGGCGTTCATGGTGGCCCTGGACAACCT
GGTGGTCACCACCGCACTCCCCCAGATCCGCGGCGACCTCGGGGCGGGCCTGGAGGGCCTCGAATGGGTGGTGAACGCCT
ACACGCTGCCGTTCGCGGTGTTGCTGCTGACCGGCGCCGCGCTCGGCGACCGGCTCGGGCGGCGGGCCGTGTTCGCCACC
GGGCTCGGGGTGTTCACCCTGGGCAGCCTCGGCGCGGCGCTCGCGCCGTCCATCGAGGCGCTCATCGCCGCCCGGGCCAT
GCAGGGCGTCGGCGCGGCGCTGGTCCTGCCACTGAGCCTGACGCTGCTGTCGGCGGCGGTCCCACCCGAGCGCCGGGGCG
CCGCGCTCGGCGTGTGGGGGGCGATGTCCGGGCTTGCCGTGGCCGTCGGGCCCCTGGTCGGTGGCGCGATCACCGACGGG
GCGAGCTGGCAGTGGATCTTCTGGCTGAACGTGCCGATCGGCCTGGTGACGGTCCCGCTCGCGCTGCGCAGGCTCACGGA
GTCCCGCGGGCCGGCCGGACGGCTGGACCTGCCGGGTGTCGCGATCATCTCCGCCGGGCTGCTCGGCGTCACACTCGGGC
TGGTCCGCGGTGAGAGCCACGGGTGGACGAGCCCGGCCGTCCTGACGTCACTGCTGGCCGGCGTGGCCGGGGTCGGCGCC
TTCATCGCCTGGGAGCGGCGCGTGGCCCGGCGCGGCGGGAGCCCCATGCTGCCGATGGCCCTGTTCCGCCGCCGCGGGTT
CGCAGCGGTGAACGCCGTGTCGCTGCTGTTCTCGGTCGGCATGTTCGGGTCGATCTTCCTGTTGGCGCAGGCCCTGCAGA
ACGTCTTCGACTACTCGCCCCTCGAGGCCGGCATCCGCACGCTGCCCTGGACGGCGATGCCCCTGCTGATCGCCCCGATC
GCCGGACCGCTCTCGGATCGGATCGGTGGGCGCCCGCTGATCGTGGCCGGTATGGCGATCGAGGCCGTGAGCCTGGCCTG
GCTCGCGACGATCCTGGACACCGGGGCCGACTACGCCGGGCTCGTCCCACCCTTCGTCCTGGCCGGGGTGGGCATGGCGC
TGTTCTTCGTTCCGGTCGCGTCGGTGGCCCTCGGTTCGGTCCCGGCGGAGCAGGAGGGTCTCGCCTCCGGCACGAACAAC
GCGATGCGCGAGCTCGGCGGCGTCCTGGGCGTCGCGGTGCTGGCCTCGGTCTTCGCCGCGAACGGCGGCTACGCGAGCCC
GGGGGCGTTCGTGGACGGCACCCGGCCCGCGCTGTGGGTGGGGGCGGCCGTCCTGCTCACCGCCGCGGCAGTGGCCACGC
TGCTGCCGTCGCCCGCGAGGACGGCAGCGCCCGCGACGCCCCCGGCACCCGCCGCGCCTGCGGCCTCCCAGGCCCTGCCC
GTGGTCGGCAGGCCGTCGTCCGCCTGA

Upstream 100 bases:

>100_bases
CACCCGTTCCACCTGTCCGCATCCTCGTTCGCACCACAAATCTAGTGACCAATCACTATCCGACAAAGGTAGTGAGCACT
CAAGACCTTGGAGGGAACTC

Downstream 100 bases:

>100_bases
AGCGGGTCCGGGCACGGATCACCGAACACGGTGATCCGTGCCCGGACGTCGTTGCACCCGCGGAATGAAAATCGATACTC
ACCGACGCGATCAGCAGATG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTDKRSATVWTFVVTSITAFMVALDNLVVTTALPQIRGDLGAGLEGLEWVVNAYTLPFAVLLLTGAALGDRLGRRAVFAT
GLGVFTLGSLGAALAPSIEALIAARAMQGVGAALVLPLSLTLLSAAVPPERRGAALGVWGAMSGLAVAVGPLVGGAITDG
ASWQWIFWLNVPIGLVTVPLALRRLTESRGPAGRLDLPGVAIISAGLLGVTLGLVRGESHGWTSPAVLTSLLAGVAGVGA
FIAWERRVARRGGSPMLPMALFRRRGFAAVNAVSLLFSVGMFGSIFLLAQALQNVFDYSPLEAGIRTLPWTAMPLLIAPI
AGPLSDRIGGRPLIVAGMAIEAVSLAWLATILDTGADYAGLVPPFVLAGVGMALFFVPVASVALGSVPAEQEGLASGTNN
AMRELGGVLGVAVLASVFAANGGYASPGAFVDGTRPALWVGAAVLLTAAAVATLLPSPARTAAPATPPAPAAPAASQALP
VVGRPSSA

Sequences:

>Translated_488_residues
MTDKRSATVWTFVVTSITAFMVALDNLVVTTALPQIRGDLGAGLEGLEWVVNAYTLPFAVLLLTGAALGDRLGRRAVFAT
GLGVFTLGSLGAALAPSIEALIAARAMQGVGAALVLPLSLTLLSAAVPPERRGAALGVWGAMSGLAVAVGPLVGGAITDG
ASWQWIFWLNVPIGLVTVPLALRRLTESRGPAGRLDLPGVAIISAGLLGVTLGLVRGESHGWTSPAVLTSLLAGVAGVGA
FIAWERRVARRGGSPMLPMALFRRRGFAAVNAVSLLFSVGMFGSIFLLAQALQNVFDYSPLEAGIRTLPWTAMPLLIAPI
AGPLSDRIGGRPLIVAGMAIEAVSLAWLATILDTGADYAGLVPPFVLAGVGMALFFVPVASVALGSVPAEQEGLASGTNN
AMRELGGVLGVAVLASVFAANGGYASPGAFVDGTRPALWVGAAVLLTAAAVATLLPSPARTAAPATPPAPAAPAASQALP
VVGRPSSA
>Mature_487_residues
TDKRSATVWTFVVTSITAFMVALDNLVVTTALPQIRGDLGAGLEGLEWVVNAYTLPFAVLLLTGAALGDRLGRRAVFATG
LGVFTLGSLGAALAPSIEALIAARAMQGVGAALVLPLSLTLLSAAVPPERRGAALGVWGAMSGLAVAVGPLVGGAITDGA
SWQWIFWLNVPIGLVTVPLALRRLTESRGPAGRLDLPGVAIISAGLLGVTLGLVRGESHGWTSPAVLTSLLAGVAGVGAF
IAWERRVARRGGSPMLPMALFRRRGFAAVNAVSLLFSVGMFGSIFLLAQALQNVFDYSPLEAGIRTLPWTAMPLLIAPIA
GPLSDRIGGRPLIVAGMAIEAVSLAWLATILDTGADYAGLVPPFVLAGVGMALFFVPVASVALGSVPAEQEGLASGTNNA
MRELGGVLGVAVLASVFAANGGYASPGAFVDGTRPALWVGAAVLLTAAAVATLLPSPARTAAPATPPAPAAPAASQALPV
VGRPSSA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=414, Percent_Identity=29.7101449275362, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789042, Length=407, Percent_Identity=29.97542997543, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=32.6086956521739, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790195, Length=439, Percent_Identity=28.0182232346241, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788392, Length=429, Percent_Identity=28.9044289044289, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1790146, Length=157, Percent_Identity=27.3885350318471, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=368, Percent_Identity=26.3586956521739, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321663, Length=369, Percent_Identity=23.0352303523035, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=30.2013422818792, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=394, Percent_Identity=24.6192893401015, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=440, Percent_Identity=23.4090909090909, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=295, Percent_Identity=23.728813559322, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49238; Mature: 49107

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKRSATVWTFVVTSITAFMVALDNLVVTTALPQIRGDLGAGLEGLEWVVNAYTLPFAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLLTGAALGDRLGRRAVFATGLGVFTLGSLGAALAPSIEALIAARAMQGVGAALVLPLSL
HHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLLSAAVPPERRGAALGVWGAMSGLAVAVGPLVGGAITDGASWQWIFWLNVPIGLVTVPL
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
ALRRLTESRGPAGRLDLPGVAIISAGLLGVTLGLVRGESHGWTSPAVLTSLLAGVAGVGA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FIAWERRVARRGGSPMLPMALFRRRGFAAVNAVSLLFSVGMFGSIFLLAQALQNVFDYSP
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LEAGIRTLPWTAMPLLIAPIAGPLSDRIGGRPLIVAGMAIEAVSLAWLATILDTGADYAG
HHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
LVPPFVLAGVGMALFFVPVASVALGSVPAEQEGLASGTNNAMRELGGVLGVAVLASVFAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NGGYASPGAFVDGTRPALWVGAAVLLTAAAVATLLPSPARTAAPATPPAPAAPAASQALP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VVGRPSSA
CCCCCCCC
>Mature Secondary Structure 
TDKRSATVWTFVVTSITAFMVALDNLVVTTALPQIRGDLGAGLEGLEWVVNAYTLPFAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLLTGAALGDRLGRRAVFATGLGVFTLGSLGAALAPSIEALIAARAMQGVGAALVLPLSL
HHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLLSAAVPPERRGAALGVWGAMSGLAVAVGPLVGGAITDGASWQWIFWLNVPIGLVTVPL
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
ALRRLTESRGPAGRLDLPGVAIISAGLLGVTLGLVRGESHGWTSPAVLTSLLAGVAGVGA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FIAWERRVARRGGSPMLPMALFRRRGFAAVNAVSLLFSVGMFGSIFLLAQALQNVFDYSP
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LEAGIRTLPWTAMPLLIAPIAGPLSDRIGGRPLIVAGMAIEAVSLAWLATILDTGADYAG
HHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
LVPPFVLAGVGMALFFVPVASVALGSVPAEQEGLASGTNNAMRELGGVLGVAVLASVFAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NGGYASPGAFVDGTRPALWVGAAVLLTAAAVATLLPSPARTAAPATPPAPAAPAASQALP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VVGRPSSA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]