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The map label for this gene is otsA [H]

Identifier: 158316749

GI number: 158316749

Start: 5982100

End: 5983620

Strand: Reverse

Name: otsA [H]

Synonym: Franean1_4987

Alternate gene names: 158316749

Gene position: 5983620-5982100 (Counterclockwise)

Preceding gene: 158316750

Following gene: 158316748

Centisome position: 66.62

GC content: 69.3

Gene sequence:

>1521_bases
GTGTCGGACATCGACCTGCTCGATCTTCCGCTGGTTCTGCTGTCCAACCGAGGTCCTGTCAGCTTCGATCGTGACGCGTC
CGGCCGGACCGTCAACCGTGGCGCCGGCGGGCTGGTGTCCGCGCTGTCCGGCCTCGCCGAGCACCTCGACGACGCGGTCT
GGGTGTGCGGCGCCGCGACCCCCGAGGACGCCGCCGTCGCCGGTGAGAACGACGGCGGCGCGATCCTGGTGCAGACCCAG
CCGACCCCGCACGTCGTCGAGGGGCAGCCGGACGGCCCGTCGATCAGGGTCCGGTTGGTGGTGACCGATCCGGACGCCTA
CGCCGACTTCTACAGCGTCTGGTCGAACCCGATCCTGTGGTTCATCCAGCACGGCCTGTACGGCCGGTCGCTGGCTCCGG
TGCTCACCCGGGCCGAGCACGACGCGTTCGAGAACGGCTACGCCGCGGTGAATCGGGCCTACGCCGACGCCGTCGCCGAC
GAGGTTCAGTCCCGCGGCGGCAAGGCGCTGGTGCTGCTGCAGGACTACCACTTCTACCTGGTGGCCGAACACGTCCGCGA
ACAGTGCCCCGATGTGGTTCTCACCCACTTCGTGCATATTCCGTGGCCGGGCCCGGACGAATGGCGCGTGCTGCCCGGGA
ACATCCGGGAACGTCTGCTGCGCGGCCTGCTCGGCAGTGACGTCGTCGGCTTTCACACCCGCCGTTACGCCCGGAACTTC
GTGCTGTGCGCCGGCGAGCTGCTCGGCCTGCCGATCAACCTCGACGACCTGACCGTCACGGTGAACAACCGGGTGGTGAG
CGCCCGCTACTATCCGATCTCCGTCGACCCGGCGGCCCTCGACGCCACGCTGGCCAGCGAGGACGTCGCCGCCCACATGG
CGATGCTGCGCTACGTGTTCCTCGACGAGGACCGCCAGCTCGTGCTCCGGGTGGACCGCACCGACCCCAGCAAGAACGTG
GTGCGCGGTTTCCTGGCCTTCGGCACCCTGCTCGACCAGCATCCCGAGCTCGTCGGGCGGGTCAGCTTCCTCGCGCTGCT
GCAGCCGAGCCGGACCGACGTCCCGGAGTACGCCGACTACATCGCGCTGATCGGCGCGGTCGTCGCTGAGATCAACGCCA
AGCACACCAAGGAGGGGCGCCAGCCCATCGATCTCCGCATGGTGGAGGACTTCGCGCTGGCGGTCGCCGCCTACTCGATC
TGTGACGTGCTGATGGTCAACGCCATCGCCGACGGCATGAACCTCGTGGCCAAGGAGGTCGCCGTCGTCAACCGGCGCGG
CGGCGTGCTCGCGCTGTCCGAGATGGCCGGGTCGCACGAGGAGCTGGGTGAGTTCGCCGTCACGCTGCACCCGTTCGACG
TCCAGCAGATGGCCGACGCCCTCTACACCGCGCTCACGATGCCGATGGAGGAGCGGCACCGCCGGCTCGCCGCGGCCGCC
GAGCAGGTCCGCACGCACGACGTCCGGCGATGGCTCAACGACCAACTGGCGGATCTGCGTCACCTGGCTGGATTCTCCTG
A

Upstream 100 bases:

>100_bases
CCGACCCTTCGGCGGGCCGTGGCCGACAGTGCCCACATTGCCGGCCGTGCGCCGACCGACGGTGCCGGGCACCATGACGA
CCAGGAGGGCTTCTTCCGCC

Downstream 100 bases:

>100_bases
GACGTTCCCGGGCGGGTACGCTCGGCGGGAGGTCGGGCATGGTTACCGTGGACGACACGGTTACCGCAGACCACAAGACA
GGAGTGGACGTGTCGGAGAC

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSDIDLLDLPLVLLSNRGPVSFDRDASGRTVNRGAGGLVSALSGLAEHLDDAVWVCGAATPEDAAVAGENDGGAILVQTQ
PTPHVVEGQPDGPSIRVRLVVTDPDAYADFYSVWSNPILWFIQHGLYGRSLAPVLTRAEHDAFENGYAAVNRAYADAVAD
EVQSRGGKALVLLQDYHFYLVAEHVREQCPDVVLTHFVHIPWPGPDEWRVLPGNIRERLLRGLLGSDVVGFHTRRYARNF
VLCAGELLGLPINLDDLTVTVNNRVVSARYYPISVDPAALDATLASEDVAAHMAMLRYVFLDEDRQLVLRVDRTDPSKNV
VRGFLAFGTLLDQHPELVGRVSFLALLQPSRTDVPEYADYIALIGAVVAEINAKHTKEGRQPIDLRMVEDFALAVAAYSI
CDVLMVNAIADGMNLVAKEVAVVNRRGGVLALSEMAGSHEELGEFAVTLHPFDVQQMADALYTALTMPMEERHRRLAAAA
EQVRTHDVRRWLNDQLADLRHLAGFS

Sequences:

>Translated_506_residues
MSDIDLLDLPLVLLSNRGPVSFDRDASGRTVNRGAGGLVSALSGLAEHLDDAVWVCGAATPEDAAVAGENDGGAILVQTQ
PTPHVVEGQPDGPSIRVRLVVTDPDAYADFYSVWSNPILWFIQHGLYGRSLAPVLTRAEHDAFENGYAAVNRAYADAVAD
EVQSRGGKALVLLQDYHFYLVAEHVREQCPDVVLTHFVHIPWPGPDEWRVLPGNIRERLLRGLLGSDVVGFHTRRYARNF
VLCAGELLGLPINLDDLTVTVNNRVVSARYYPISVDPAALDATLASEDVAAHMAMLRYVFLDEDRQLVLRVDRTDPSKNV
VRGFLAFGTLLDQHPELVGRVSFLALLQPSRTDVPEYADYIALIGAVVAEINAKHTKEGRQPIDLRMVEDFALAVAAYSI
CDVLMVNAIADGMNLVAKEVAVVNRRGGVLALSEMAGSHEELGEFAVTLHPFDVQQMADALYTALTMPMEERHRRLAAAA
EQVRTHDVRRWLNDQLADLRHLAGFS
>Mature_505_residues
SDIDLLDLPLVLLSNRGPVSFDRDASGRTVNRGAGGLVSALSGLAEHLDDAVWVCGAATPEDAAVAGENDGGAILVQTQP
TPHVVEGQPDGPSIRVRLVVTDPDAYADFYSVWSNPILWFIQHGLYGRSLAPVLTRAEHDAFENGYAAVNRAYADAVADE
VQSRGGKALVLLQDYHFYLVAEHVREQCPDVVLTHFVHIPWPGPDEWRVLPGNIRERLLRGLLGSDVVGFHTRRYARNFV
LCAGELLGLPINLDDLTVTVNNRVVSARYYPISVDPAALDATLASEDVAAHMAMLRYVFLDEDRQLVLRVDRTDPSKNVV
RGFLAFGTLLDQHPELVGRVSFLALLQPSRTDVPEYADYIALIGAVVAEINAKHTKEGRQPIDLRMVEDFALAVAAYSIC
DVLMVNAIADGMNLVAKEVAVVNRRGGVLALSEMAGSHEELGEFAVTLHPFDVQQMADALYTALTMPMEERHRRLAAAAE
QVRTHDVRRWLNDQLADLRHLAGFS

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=397, Percent_Identity=28.463476070529, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71987012, Length=556, Percent_Identity=23.2014388489209, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115533560, Length=485, Percent_Identity=24.9484536082474, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115533558, Length=485, Percent_Identity=24.9484536082474, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=28.7234042553192, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323917, Length=395, Percent_Identity=28.6075949367089, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323537, Length=403, Percent_Identity=28.287841191067, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320279, Length=416, Percent_Identity=24.0384615384615, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19920676, Length=508, Percent_Identity=30.7086614173228, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 55485; Mature: 55353

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIDLLDLPLVLLSNRGPVSFDRDASGRTVNRGAGGLVSALSGLAEHLDDAVWVCGAAT
CCCCCHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEECCCC
PEDAAVAGENDGGAILVQTQPTPHVVEGQPDGPSIRVRLVVTDPDAYADFYSVWSNPILW
CCCCEECCCCCCCEEEEECCCCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHH
FIQHGLYGRSLAPVLTRAEHDAFENGYAAVNRAYADAVADEVQSRGGKALVLLQDYHFYL
HHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
VAEHVREQCPDVVLTHFVHIPWPGPDEWRVLPGNIRERLLRGLLGSDVVGFHTRRYARNF
HHHHHHHHCCHHHHHHEEECCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLCAGELLGLPINLDDLTVTVNNRVVSARYYPISVDPAALDATLASEDVAAHMAMLRYVF
EEEEHHHHCCCCCCCCEEEEECCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LDEDRQLVLRVDRTDPSKNVVRGFLAFGTLLDQHPELVGRVSFLALLQPSRTDVPEYADY
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
IALIGAVVAEINAKHTKEGRQPIDLRMVEDFALAVAAYSICDVLMVNAIADGMNLVAKEV
HHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVNRRGGVLALSEMAGSHEELGEFAVTLHPFDVQQMADALYTALTMPMEERHRRLAAAA
HHHCCCCCEEEEHHHCCCHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
EQVRTHDVRRWLNDQLADLRHLAGFS
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDIDLLDLPLVLLSNRGPVSFDRDASGRTVNRGAGGLVSALSGLAEHLDDAVWVCGAAT
CCCCHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEECCCC
PEDAAVAGENDGGAILVQTQPTPHVVEGQPDGPSIRVRLVVTDPDAYADFYSVWSNPILW
CCCCEECCCCCCCEEEEECCCCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHH
FIQHGLYGRSLAPVLTRAEHDAFENGYAAVNRAYADAVADEVQSRGGKALVLLQDYHFYL
HHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
VAEHVREQCPDVVLTHFVHIPWPGPDEWRVLPGNIRERLLRGLLGSDVVGFHTRRYARNF
HHHHHHHHCCHHHHHHEEECCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLCAGELLGLPINLDDLTVTVNNRVVSARYYPISVDPAALDATLASEDVAAHMAMLRYVF
EEEEHHHHCCCCCCCCEEEEECCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LDEDRQLVLRVDRTDPSKNVVRGFLAFGTLLDQHPELVGRVSFLALLQPSRTDVPEYADY
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
IALIGAVVAEINAKHTKEGRQPIDLRMVEDFALAVAAYSICDVLMVNAIADGMNLVAKEV
HHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVNRRGGVLALSEMAGSHEELGEFAVTLHPFDVQQMADALYTALTMPMEERHRRLAAAA
HHHCCCCCEEEEHHHCCCHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
EQVRTHDVRRWLNDQLADLRHLAGFS
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA