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The map label for this gene is hyuB [H]

Identifier: 158316589

GI number: 158316589

Start: 5762339

End: 5764099

Strand: Reverse

Name: hyuB [H]

Synonym: Franean1_4825

Alternate gene names: 158316589

Gene position: 5764099-5762339 (Counterclockwise)

Preceding gene: 158316590

Following gene: 158316588

Centisome position: 64.17

GC content: 73.14

Gene sequence:

>1761_bases
ATGACCGGCGCGGACCCGGTGCTGGCCGGGATCGTCGACAACCGGCTGCACGCGATCGCCGAGCAGATGGCGCAGGCCAT
GCTGCGTTCCTGCCGGTCGATGGTGTTCCAGAGCCGGGACTTCGTCACCGGGATCTTCACCGCCCGAGGCGAGTGGGTCG
CGACCAAGGACTACATCCCCGTCCTGGCCGGTTCGCTGCCCAGCGCCCTCGCCGGCGTGACCGAGCGCTTCGCCGCCGAG
TTCGCGGCCGGGGACCTGTACGAGGGCGACGTGTTCGTCCTCAACGATCCGTACCACGGCAACAACCACCCGCCGGATAT
CACGATCATGAAGCCGGTGTTCCACCGGGGCGTGCACCGATTCTGGACCGTGACGAAGGGGCATCACGCCGACACCGGCG
GCGCCGTCGTCGGCTACAACCCGTACGCCCGGGACTGCTGGGAGGACGCACTGCGCATCCCGCCCGTCCGGCTGCACCAC
CGCGGGCGGCGCCAGCGGGACGTCTGGGATCTCATCCTGCTGAACCTACGGCTGCCGGACGTCGTCGAGGCCGACCTGGA
CTGCCAGATCGGCGCCGCCACGCTCGGCGAGCGCGAGCTGGTCGCGCTGCTGGACGCCTACGGCGTCGAACCGGTCGAGA
ACGCCGTCGCCTACCAGCTCGACGCGTCCCGCCGGCACATGCGAGCCGAGATCGGACGGCTGCCGGACGGCGTCTACCGG
TCGGTGCGCCACCTCGACGACGGGGGAGCACACCACCGCGAGCCGATCGCGGTGCGCCTGGAGCTGACCATCCGCGGCGA
GTCGGCGCTGTTCGACTTCACCGCCTCCGACCCGCAGGTCGTCGGCTACGCGAACTCCACCCCGGCGAACACCGCCGCCG
CCGTCCTCATCGGCCTGCTCGGGGTCACCGACCCCACCCAACGCCGCACCAGCGGCGCGCTGGCGGCCCTCGACATCCGC
ACCACGCCGGGCACGATCGTGCACGCCGTCGAGCCGGCCGCGACGTCGCTGTGCACGCTGACCGCCTGCGAGACCATCGT
CGAGACGGTCTGGGAGGCCGTGGGGCAGGCCGACCCGACGGTGACGAACGCCGCCTGGTGCCGTGGCTACACGTTCGCGG
CCATGGGGGTCGACGCGCGCACCGGCCGGCCGTTCGCCGTGACCGGCGCGATGAAGGGCGGCTCGGGCGCCACCGCCGGT
TTCGACGGCTGGGACGCCGTGGGGCCGCCGGTCGCGATGGGCGGCGGACGGGAGATCGACGTCGAGCTGCACGAGCTGAC
CGCGCCGACCACCGTGCTCAGCCAGGAGTACGAGACCGACTCCGCCGGCGCCGGCCGGTGGCGCGGCGGCCTCGGCGCGG
TGTCGCGATGGCGTATTGACCAGGACGACCTGTCGGTGCTGTGCATCGGATCAGGTGCCCTCGACCTGACAGCGCCGTTC
GGCGCGGCGGGCGGGGCGCCCGCCCCCGCCAACCGCGGCATCGTCCACCACGCCGACGGGCGGATCAGCCGCCCCCCGAC
GAACTCCCTGCTACGGCTTAACCGCGGGGACGTGGTCGAGATCCACACCTCCGGCGGAGGCGGTTTCGGCGACCCCCTCG
ACCGTCCCGTCGACGCGGTCGCCGACGACGTCCGCGACGGCCTCGTCTCGCCGACGGCCGCCCGCGATGTCTACGGCGTC
ATCCTCGACCCGATCACCGATGAGGTGGACGCCGCCGCGACCGCCGCCCGCCGCGCGGCAGCCCGCTCCCGCTCCCGCTG
A

Upstream 100 bases:

>100_bases
CCGTCCTGGTCTTCGCGGCCACTGACACCGCGGACGCCGTCCTGCACCGGCTCGGGCTCGTCGGCGTCGCCGGCGCCGGC
GACGCCGACGCGGCGGCCCG

Downstream 100 bases:

>100_bases
GGCTGGCCAGTCCGGCCGGCATCCGTTGAGAATTTTTTTTCTGCCGAGTGATGGCAGAGCCGCCAAACCTCGGCCAGCCT
AATGTTCGGCTGCGTGACAT

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTGADPVLAGIVDNRLHAIAEQMAQAMLRSCRSMVFQSRDFVTGIFTARGEWVATKDYIPVLAGSLPSALAGVTERFAAE
FAAGDLYEGDVFVLNDPYHGNNHPPDITIMKPVFHRGVHRFWTVTKGHHADTGGAVVGYNPYARDCWEDALRIPPVRLHH
RGRRQRDVWDLILLNLRLPDVVEADLDCQIGAATLGERELVALLDAYGVEPVENAVAYQLDASRRHMRAEIGRLPDGVYR
SVRHLDDGGAHHREPIAVRLELTIRGESALFDFTASDPQVVGYANSTPANTAAAVLIGLLGVTDPTQRRTSGALAALDIR
TTPGTIVHAVEPAATSLCTLTACETIVETVWEAVGQADPTVTNAAWCRGYTFAAMGVDARTGRPFAVTGAMKGGSGATAG
FDGWDAVGPPVAMGGGREIDVELHELTAPTTVLSQEYETDSAGAGRWRGGLGAVSRWRIDQDDLSVLCIGSGALDLTAPF
GAAGGAPAPANRGIVHHADGRISRPPTNSLLRLNRGDVVEIHTSGGGGFGDPLDRPVDAVADDVRDGLVSPTAARDVYGV
ILDPITDEVDAAATAARRAAARSRSR

Sequences:

>Translated_586_residues
MTGADPVLAGIVDNRLHAIAEQMAQAMLRSCRSMVFQSRDFVTGIFTARGEWVATKDYIPVLAGSLPSALAGVTERFAAE
FAAGDLYEGDVFVLNDPYHGNNHPPDITIMKPVFHRGVHRFWTVTKGHHADTGGAVVGYNPYARDCWEDALRIPPVRLHH
RGRRQRDVWDLILLNLRLPDVVEADLDCQIGAATLGERELVALLDAYGVEPVENAVAYQLDASRRHMRAEIGRLPDGVYR
SVRHLDDGGAHHREPIAVRLELTIRGESALFDFTASDPQVVGYANSTPANTAAAVLIGLLGVTDPTQRRTSGALAALDIR
TTPGTIVHAVEPAATSLCTLTACETIVETVWEAVGQADPTVTNAAWCRGYTFAAMGVDARTGRPFAVTGAMKGGSGATAG
FDGWDAVGPPVAMGGGREIDVELHELTAPTTVLSQEYETDSAGAGRWRGGLGAVSRWRIDQDDLSVLCIGSGALDLTAPF
GAAGGAPAPANRGIVHHADGRISRPPTNSLLRLNRGDVVEIHTSGGGGFGDPLDRPVDAVADDVRDGLVSPTAARDVYGV
ILDPITDEVDAAATAARRAAARSRSR
>Mature_585_residues
TGADPVLAGIVDNRLHAIAEQMAQAMLRSCRSMVFQSRDFVTGIFTARGEWVATKDYIPVLAGSLPSALAGVTERFAAEF
AAGDLYEGDVFVLNDPYHGNNHPPDITIMKPVFHRGVHRFWTVTKGHHADTGGAVVGYNPYARDCWEDALRIPPVRLHHR
GRRQRDVWDLILLNLRLPDVVEADLDCQIGAATLGERELVALLDAYGVEPVENAVAYQLDASRRHMRAEIGRLPDGVYRS
VRHLDDGGAHHREPIAVRLELTIRGESALFDFTASDPQVVGYANSTPANTAAAVLIGLLGVTDPTQRRTSGALAALDIRT
TPGTIVHAVEPAATSLCTLTACETIVETVWEAVGQADPTVTNAAWCRGYTFAAMGVDARTGRPFAVTGAMKGGSGATAGF
DGWDAVGPPVAMGGGREIDVELHELTAPTTVLSQEYETDSAGAGRWRGGLGAVSRWRIDQDDLSVLCIGSGALDLTAPFG
AAGGAPAPANRGIVHHADGRISRPPTNSLLRLNRGDVVEIHTSGGGGFGDPLDRPVDAVADDVRDGLVSPTAARDVYGVI
LDPITDEVDAAATAARRAAARSRSR

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=555, Percent_Identity=27.5675675675676, Blast_Score=131, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI133901900, Length=558, Percent_Identity=25.8064516129032, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133901902, Length=526, Percent_Identity=25.2851711026616, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322634, Length=561, Percent_Identity=25.4901960784314, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI45550492, Length=561, Percent_Identity=26.2032085561497, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 62236; Mature: 62104

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGADPVLAGIVDNRLHAIAEQMAQAMLRSCRSMVFQSRDFVTGIFTARGEWVATKDYIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHH
VLAGSLPSALAGVTERFAAEFAAGDLYEGDVFVLNDPYHGNNHPPDITIMKPVFHRGVHR
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEECHHHHCCHHH
FWTVTKGHHADTGGAVVGYNPYARDCWEDALRIPPVRLHHRGRRQRDVWDLILLNLRLPD
HEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCH
VVEADLDCQIGAATLGERELVALLDAYGVEPVENAVAYQLDASRRHMRAEIGRLPDGVYR
HHCCCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHEEEECHHHHHHHHHHHCCCHHHHH
SVRHLDDGGAHHREPIAVRLELTIRGESALFDFTASDPQVVGYANSTPANTAAAVLIGLL
HHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHH
GVTDPTQRRTSGALAALDIRTTPGTIVHAVEPAATSLCTLTACETIVETVWEAVGQADPT
CCCCCHHHHCCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VTNAAWCRGYTFAAMGVDARTGRPFAVTGAMKGGSGATAGFDGWDAVGPPVAMGGGREID
CCCHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCEEE
VELHELTAPTTVLSQEYETDSAGAGRWRGGLGAVSRWRIDQDDLSVLCIGSGALDLTAPF
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCEEECCCC
GAAGGAPAPANRGIVHHADGRISRPPTNSLLRLNRGDVVEIHTSGGGGFGDPLDRPVDAV
CCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHH
ADDVRDGLVSPTAARDVYGVILDPITDEVDAAATAARRAAARSRSR
HHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGADPVLAGIVDNRLHAIAEQMAQAMLRSCRSMVFQSRDFVTGIFTARGEWVATKDYIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHH
VLAGSLPSALAGVTERFAAEFAAGDLYEGDVFVLNDPYHGNNHPPDITIMKPVFHRGVHR
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEECHHHHCCHHH
FWTVTKGHHADTGGAVVGYNPYARDCWEDALRIPPVRLHHRGRRQRDVWDLILLNLRLPD
HEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCH
VVEADLDCQIGAATLGERELVALLDAYGVEPVENAVAYQLDASRRHMRAEIGRLPDGVYR
HHCCCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHEEEECHHHHHHHHHHHCCCHHHHH
SVRHLDDGGAHHREPIAVRLELTIRGESALFDFTASDPQVVGYANSTPANTAAAVLIGLL
HHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHH
GVTDPTQRRTSGALAALDIRTTPGTIVHAVEPAATSLCTLTACETIVETVWEAVGQADPT
CCCCCHHHHCCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VTNAAWCRGYTFAAMGVDARTGRPFAVTGAMKGGSGATAGFDGWDAVGPPVAMGGGREID
CCCHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCEEE
VELHELTAPTTVLSQEYETDSAGAGRWRGGLGAVSRWRIDQDDLSVLCIGSGALDLTAPF
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCEEECCCC
GAAGGAPAPANRGIVHHADGRISRPPTNSLLRLNRGDVVEIHTSGGGGFGDPLDRPVDAV
CCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHH
ADDVRDGLVSPTAARDVYGVILDPITDEVDAAATAARRAAARSRSR
HHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]