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The map label for this gene is acoB [H]

Identifier: 158316486

GI number: 158316486

Start: 5635419

End: 5636474

Strand: Reverse

Name: acoB [H]

Synonym: Franean1_4721

Alternate gene names: 158316486

Gene position: 5636474-5635419 (Counterclockwise)

Preceding gene: 158316487

Following gene: 158316485

Centisome position: 62.75

GC content: 65.25

Gene sequence:

>1056_bases
ATGACTGTTCGCCTTCTCGATCATTGGACCTGCATAGAGGTGGACGTGGACGAGCAACGGATGACGATGCGCGAGGCGTT
GAACCTCGCTTTGGACCAGGCGTTGGCGCGTGATGAGCGTGTCTTCCTGCTGGGGGAGGATATTGCCGATCCCGGCTCCA
GTGGGCCGACCAAGGGGCTGTCGACCAAATACGGCGCGGATCGGGTGCTTGACACGCCGATTTCCGAAGCGGCGATCGTG
GGTGCCGCGATCGGTGCCGCGATGGAGGGGTTCCGCCCGGTCGCCGAAATCATGATCATGGATTTTATTGGTATCGCCGC
CGATCAGATCGTCAATCATGCGGCGAAGCTGCGGTTCATGACCGGGGGGCGGACGACCGCGCCGATCACGGTCCGGACGC
AGGTCTATGGCGGGTTGGGGACCGGGGCGACGCACTCGCAGTCGTTGGAGGCGTGGTTCATGCATGTTCCGGGGCTGAAG
GTGATCGTGCCTTCGACGCCGCGGGACGCCAAGGGTCTTCTCGCGTCGGCGATTTTTGATGATGACCCGTGCGTCTTCCT
GGAGACGATTCGTCTGCAGGGCCAGCGCGGGTTGGTGCCGGTGGATCCGGGATTCTCGATTCCGCTGGGTCAGGCGGACG
TGAAACGGCCGGGTACTGATGTCACGTTGATCGGCTATGGCCGGGGGGTGGTCGAGTCGCTCGGCGCGGCGGCCGTGCTC
GAGGCGGAGGGGGTCAGCGCCGAGGTGCTTGATCTGCGCACCCTGGTTCCGCTCGATGTGCCGGCGATGGTTGATTCGGT
GCGTCGAACGAGGCGTGCTGTGGTGGTGCATGACGCGGTGCGGTTCGCCGGGCCCGGGGCGGAGATCGCCGCGATTCTGC
AGCGCGAGCTCTTCGGTGTTCTTGAGGCGCCGGTGGAGCGGGTGGGTGCCCGGTTCGTGCCGAATCCGGCCCCGCCGGCA
CTGGAATCGCAGATATACCCGAGCACCGAACGTATTGTCGCGGCGGTCCAGCAGACGCTGCGGTCGGAAACGACCAGGGA
CGGTGCCTGTGGCTGA

Upstream 100 bases:

>100_bases
TGGATCGTGACGTGTACGCCGACCCGCGGAACTGCCCCGCCTAGACCCATATCCCGCTGGCGTCACGGTAAGAGCGGCTC
CGGGCAATGCCGGCCTCGTG

Downstream 100 bases:

>100_bases
CTTCACGATCCGTATTCCGCGTGTATCGGTCGCGATCTCGGAGGCGGAGCTGATCGAGCTTCTCGTGGATGAGGGAGCGA
ACGTGTCTGAAGGCGACCCG

Product: transketolase central region

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTVRLLDHWTCIEVDVDEQRMTMREALNLALDQALARDERVFLLGEDIADPGSSGPTKGLSTKYGADRVLDTPISEAAIV
GAAIGAAMEGFRPVAEIMIMDFIGIAADQIVNHAAKLRFMTGGRTTAPITVRTQVYGGLGTGATHSQSLEAWFMHVPGLK
VIVPSTPRDAKGLLASAIFDDDPCVFLETIRLQGQRGLVPVDPGFSIPLGQADVKRPGTDVTLIGYGRGVVESLGAAAVL
EAEGVSAEVLDLRTLVPLDVPAMVDSVRRTRRAVVVHDAVRFAGPGAEIAAILQRELFGVLEAPVERVGARFVPNPAPPA
LESQIYPSTERIVAAVQQTLRSETTRDGACG

Sequences:

>Translated_351_residues
MTVRLLDHWTCIEVDVDEQRMTMREALNLALDQALARDERVFLLGEDIADPGSSGPTKGLSTKYGADRVLDTPISEAAIV
GAAIGAAMEGFRPVAEIMIMDFIGIAADQIVNHAAKLRFMTGGRTTAPITVRTQVYGGLGTGATHSQSLEAWFMHVPGLK
VIVPSTPRDAKGLLASAIFDDDPCVFLETIRLQGQRGLVPVDPGFSIPLGQADVKRPGTDVTLIGYGRGVVESLGAAAVL
EAEGVSAEVLDLRTLVPLDVPAMVDSVRRTRRAVVVHDAVRFAGPGAEIAAILQRELFGVLEAPVERVGARFVPNPAPPA
LESQIYPSTERIVAAVQQTLRSETTRDGACG
>Mature_350_residues
TVRLLDHWTCIEVDVDEQRMTMREALNLALDQALARDERVFLLGEDIADPGSSGPTKGLSTKYGADRVLDTPISEAAIVG
AAIGAAMEGFRPVAEIMIMDFIGIAADQIVNHAAKLRFMTGGRTTAPITVRTQVYGGLGTGATHSQSLEAWFMHVPGLKV
IVPSTPRDAKGLLASAIFDDDPCVFLETIRLQGQRGLVPVDPGFSIPLGQADVKRPGTDVTLIGYGRGVVESLGAAAVLE
AEGVSAEVLDLRTLVPLDVPAMVDSVRRTRRAVVVHDAVRFAGPGAEIAAILQRELFGVLEAPVERVGARFVPNPAPPAL
ESQIYPSTERIVAAVQQTLRSETTRDGACG

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=339, Percent_Identity=41.2979351032448, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI291084858, Length=339, Percent_Identity=39.8230088495575, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=41.2844036697248, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=41.2844036697248, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=45.398773006135, Blast_Score=270, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17506935, Length=333, Percent_Identity=39.3393393393393, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=41.0876132930514, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=44.3425076452599, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=44.3425076452599, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI160714828, Length=324, Percent_Identity=38.8888888888889, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI160714832, Length=324, Percent_Identity=38.8888888888889, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24650943, Length=100, Percent_Identity=50, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24650945, Length=100, Percent_Identity=50, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 37322; Mature: 37191

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLLDHWTCIEVDVDEQRMTMREALNLALDQALARDERVFLLGEDIADPGSSGPTKGL
CEEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
STKYGADRVLDTPISEAAIVGAAIGAAMEGFRPVAEIMIMDFIGIAADQIVNHAAKLRFM
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
TGGRTTAPITVRTQVYGGLGTGATHSQSLEAWFMHVPGLKVIVPSTPRDAKGLLASAIFD
ECCCEECCEEEEEEEECCCCCCCCCCHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCC
DDPCVFLETIRLQGQRGLVPVDPGFSIPLGQADVKRPGTDVTLIGYGRGVVESLGAAAVL
CCCEEEEEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEE
EAEGVSAEVLDLRTLVPLDVPAMVDSVRRTRRAVVVHDAVRFAGPGAEIAAILQRELFGV
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEHHHHHCCCCHHHHHHHHHHHHHH
LEAPVERVGARFVPNPAPPALESQIYPSTERIVAAVQQTLRSETTRDGACG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVRLLDHWTCIEVDVDEQRMTMREALNLALDQALARDERVFLLGEDIADPGSSGPTKGL
EEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
STKYGADRVLDTPISEAAIVGAAIGAAMEGFRPVAEIMIMDFIGIAADQIVNHAAKLRFM
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
TGGRTTAPITVRTQVYGGLGTGATHSQSLEAWFMHVPGLKVIVPSTPRDAKGLLASAIFD
ECCCEECCEEEEEEEECCCCCCCCCCHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCC
DDPCVFLETIRLQGQRGLVPVDPGFSIPLGQADVKRPGTDVTLIGYGRGVVESLGAAAVL
CCCEEEEEEHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEE
EAEGVSAEVLDLRTLVPLDVPAMVDSVRRTRRAVVVHDAVRFAGPGAEIAAILQRELFGV
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEHHHHHCCCCHHHHHHHHHHHHHH
LEAPVERVGARFVPNPAPPALESQIYPSTERIVAAVQQTLRSETTRDGACG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]