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The map label for this gene is acoA [H]

Identifier: 158316487

GI number: 158316487

Start: 5636531

End: 5637529

Strand: Reverse

Name: acoA [H]

Synonym: Franean1_4722

Alternate gene names: 158316487

Gene position: 5637529-5636531 (Counterclockwise)

Preceding gene: 158316488

Following gene: 158316486

Centisome position: 62.76

GC content: 68.37

Gene sequence:

>999_bases
ATGACGACGGATCGAACGGCGGGCATCTCGGGACAGGTGTGGCGCCGGTTATACGAGCTGATGACGCTCATGAAGGCGGC
CGACGACCGGCTGAGCAGGGGGATCGCGTCGGGTGAGCTGCAGTGCGTGTACTGGCCTCCTCGTGGTCAGGAGGCGATTG
CCGCGGCGATGGGGGTGTGTCTTCGGTCGGACGATCAGTTGGTCACGACCTATCGGGGGTTGCATGACCTGATCGGCAAG
GGTGTGCCGTTGGTGGAGATCTACGGAGAGATGCTGGGCCGGCAGGTGGGATCGGGCCGGGGTAAGGGCGGGACGATGCA
CATTGCCCGCCCCGACTCAGGCGTCATGTTGTCGACGGGGATTGTGGGATCCGGGCCGCCGGTCGCGGTGGGGATGGCCA
TGGCGGCGCGGCGCAAGGGCCTGGATCGGGTGACGGCCGTGAGTTTCGGTGACGGGGCGACGAACACCGGCTCGTTTCAC
GAGGCGGCGAACATGGCGGCGCTGTGGGATCTGCCGTTGGTGCTGGTGTGCCAGAACAACCAGTACGGCGAGATGACGCC
AACTGAGCACACGATGAAGATCGCCCAGGTCGCGGACCGGGCGGGCGGGTACGGGATGCCGGGTGTCCGCGTCGACGGCA
ACGACCCTCTCGCCGTCCTTGCCGTGTTGACGCAGGCCGTGGAGCGGGCGAGGAGCGGAGGGGGCCCGACGCTGGTGGAG
TGCGTGACGTTCCGGTTCCGTGGTCACTACTTCGGGGATCCCATGGCGTACATCCCAGCCGAACGGATGGCCGCCGCTGT
CGAGGCTGATCCGATCCCGCGGTTCCGGTCGCGCCTGCTGGAGACGGGGGTCTGCGATGAGCACGAGCTCGACGAGATCG
AGGCGGCCGCCGTCGCGGCGGTCGAGGAGGCGTTGACCGCGGTCCTGGCGGCGCCGGTCGCGGCACTCGACGAGCTGGAT
CGTGACGTGTACGCCGACCCGCGGAACTGCCCCGCCTAG

Upstream 100 bases:

>100_bases
CGCGGCTCGTCACCCCGACAGACATGCACGAGGCTCCCGCCTCTCAGCCGCTTCGCGTGTCGCCGTCTGGCGGTGGTGCA
CCGGTCGTAGGAGGAGGAAC

Downstream 100 bases:

>100_bases
ACCCATATCCCGCTGGCGTCACGGTAAGAGCGGCTCCGGGCAATGCCGGCCTCGTGATGACTGTTCGCCTTCTCGATCAT
TGGACCTGCATAGAGGTGGA

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTTDRTAGISGQVWRRLYELMTLMKAADDRLSRGIASGELQCVYWPPRGQEAIAAAMGVCLRSDDQLVTTYRGLHDLIGK
GVPLVEIYGEMLGRQVGSGRGKGGTMHIARPDSGVMLSTGIVGSGPPVAVGMAMAARRKGLDRVTAVSFGDGATNTGSFH
EAANMAALWDLPLVLVCQNNQYGEMTPTEHTMKIAQVADRAGGYGMPGVRVDGNDPLAVLAVLTQAVERARSGGGPTLVE
CVTFRFRGHYFGDPMAYIPAERMAAAVEADPIPRFRSRLLETGVCDEHELDEIEAAAVAAVEEALTAVLAAPVAALDELD
RDVYADPRNCPA

Sequences:

>Translated_332_residues
MTTDRTAGISGQVWRRLYELMTLMKAADDRLSRGIASGELQCVYWPPRGQEAIAAAMGVCLRSDDQLVTTYRGLHDLIGK
GVPLVEIYGEMLGRQVGSGRGKGGTMHIARPDSGVMLSTGIVGSGPPVAVGMAMAARRKGLDRVTAVSFGDGATNTGSFH
EAANMAALWDLPLVLVCQNNQYGEMTPTEHTMKIAQVADRAGGYGMPGVRVDGNDPLAVLAVLTQAVERARSGGGPTLVE
CVTFRFRGHYFGDPMAYIPAERMAAAVEADPIPRFRSRLLETGVCDEHELDEIEAAAVAAVEEALTAVLAAPVAALDELD
RDVYADPRNCPA
>Mature_331_residues
TTDRTAGISGQVWRRLYELMTLMKAADDRLSRGIASGELQCVYWPPRGQEAIAAAMGVCLRSDDQLVTTYRGLHDLIGKG
VPLVEIYGEMLGRQVGSGRGKGGTMHIARPDSGVMLSTGIVGSGPPVAVGMAMAARRKGLDRVTAVSFGDGATNTGSFHE
AANMAALWDLPLVLVCQNNQYGEMTPTEHTMKIAQVADRAGGYGMPGVRVDGNDPLAVLAVLTQAVERARSGGGPTLVEC
VTFRFRGHYFGDPMAYIPAERMAAAVEADPIPRFRSRLLETGVCDEHELDEIEAAAVAAVEEALTAVLAAPVAALDELDR
DVYADPRNCPA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=279, Percent_Identity=34.7670250896057, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI291084742, Length=283, Percent_Identity=36.3957597173145, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4505685, Length=283, Percent_Identity=36.3957597173145, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI291084744, Length=288, Percent_Identity=35.7638888888889, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI11386135, Length=314, Percent_Identity=29.6178343949045, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI258645172, Length=314, Percent_Identity=29.6178343949045, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI291084757, Length=283, Percent_Identity=32.1554770318021, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564172, Length=288, Percent_Identity=36.1111111111111, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17536047, Length=288, Percent_Identity=36.1111111111111, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI86563357, Length=313, Percent_Identity=28.1150159744409, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI86563355, Length=313, Percent_Identity=28.1150159744409, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321026, Length=287, Percent_Identity=33.7979094076655, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639748, Length=279, Percent_Identity=36.5591397849462, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24639746, Length=280, Percent_Identity=33.5714285714286, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24639740, Length=280, Percent_Identity=33.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639744, Length=280, Percent_Identity=33.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28571106, Length=280, Percent_Identity=33.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355903, Length=282, Percent_Identity=28.3687943262411, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35288; Mature: 35157

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDRTAGISGQVWRRLYELMTLMKAADDRLSRGIASGELQCVYWPPRGQEAIAAAMGVC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
LRSDDQLVTTYRGLHDLIGKGVPLVEIYGEMLGRQVGSGRGKGGTMHIARPDSGVMLSTG
HCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECC
IVGSGPPVAVGMAMAARRKGLDRVTAVSFGDGATNTGSFHEAANMAALWDLPLVLVCQNN
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCHHHHCCCCEEEEECCC
QYGEMTPTEHTMKIAQVADRAGGYGMPGVRVDGNDPLAVLAVLTQAVERARSGGGPTLVE
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
CVTFRFRGHYFGDPMAYIPAERMAAAVEADPIPRFRSRLLETGVCDEHELDEIEAAAVAA
HHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VEEALTAVLAAPVAALDELDRDVYADPRNCPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTDRTAGISGQVWRRLYELMTLMKAADDRLSRGIASGELQCVYWPPRGQEAIAAAMGVC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
LRSDDQLVTTYRGLHDLIGKGVPLVEIYGEMLGRQVGSGRGKGGTMHIARPDSGVMLSTG
HCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECC
IVGSGPPVAVGMAMAARRKGLDRVTAVSFGDGATNTGSFHEAANMAALWDLPLVLVCQNN
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCHHHHCCCCEEEEECCC
QYGEMTPTEHTMKIAQVADRAGGYGMPGVRVDGNDPLAVLAVLTQAVERARSGGGPTLVE
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
CVTFRFRGHYFGDPMAYIPAERMAAAVEADPIPRFRSRLLETGVCDEHELDEIEAAAVAA
HHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VEEALTAVLAAPVAALDELDRDVYADPRNCPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]