BacMap

Definition	Frankia sp. EAN1pec chromosome, complete genome.
Accession	NC_009921
Length	8,982,042

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The map label for this gene is caiD [H]

Identifier: 158316344

GI number: 158316344

Start: 5455978

End: 5456835

Strand: Reverse

Name: caiD [H]

Synonym: Franean1_4573

Alternate gene names: 158316344

Gene position: 5456835-5455978 (Counterclockwise)

Preceding gene: 158316346

Following gene: 158316343

Centisome position: 60.75

GC content: 73.19

Gene sequence:

>858_bases
TTGACGGCGTGCGGCGCACCTGGTGATGCTAATGCCAGAACGATGTTCTCTATAGAGAACCTGAGTAAGGAGCCTCCGCT
GCCAGCATCAGCCCCCTCGGACGACGTCCTGGTGCGCCGTGAGGACGGGGTCCTCACGATCACGATCAACCGTCCCGACC
AGCGCAACGCCGTGAACGGCGAGGTCTCCCGCGGCATCGCGGCCGCGCTCGACCTGCTCGACTCGACTCCCGAGCTGCAG
GTCGCGGTGCTGCACGGCGCCGGCCCGAGCTTCTGCGCGGGCATGGACCTCAAGGCGTTCGCCGCCGGGGAGAAGACCTC
GGACCCGGTGCGAGGCTTCGCCGGGCTGGTCGAACGGCCGCCCGCGAAGCCGTTGATCGCGGCGGTCGAGGGGTGGGCGT
TGGGCGGCGGTTTCGAGATCGTCCTCGCGTGCGACCTGGTGGTGGCGGGCCGCGGGGCGCGGTTCGGGCTGCCGGAGGTC
CGTCGCGGCCTGGCCGCGCGCGGCGGCGGTGCCTTCCGCCTGCCGCGCCGGCTGCCGCACATGCTCGCCATGGAACTGCT
CCTCACCGGCGCCCCGCTCGAGGCCGAGCGCGCGGCTCACTTCGGCCTGGTGAACCGGCTGGTCGACGACGGCGGGGCGC
TGGACGCCGCCCTGGCGCTCGCGGGCGTGGTCGCCGAGAGCGCGCCGATGTCGGTGCGGGCGTCCAAGCAGGTCGCGGTG
GACTCCGCCGACTGGCCGGTCAGCGAGGGTTTCGACCAGCAGCGGGCGCTGTTCGAACCGGTGTTCGCCTCCAGCGACGC
GGCCGAGGGCGTGGCCGCCTTCCGGGAGCGGCGCGCTCCGGTCTGGCGCGACGCATGA

Upstream 100 bases:

>100_bases
AAGGCCCGCTCGATCGCCGGCACCCGCGAGGCGGGGGCGTGGAGTTCGTGGCCGGTCCGGCTGACTCGGTCCGGCATTCG
TCCTCCTCGAAGGTGCGGTC

Downstream 100 bases:

>100_bases
CGCAGGCGCTGGGCGACGTCACGGTCGTGTGCCTCAGCGCGCTCGGGCCGGTCCCGTTCGCGACCATGCTGCTCGCCGAC
CTGGGCGCCCGGGTGATCCG

Product: enoyl-CoA hydratase/isomerase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTACGAPGDANARTMFSIENLSKEPPLPASAPSDDVLVRREDGVLTITINRPDQRNAVNGEVSRGIAAALDLLDSTPELQ
VAVLHGAGPSFCAGMDLKAFAAGEKTSDPVRGFAGLVERPPAKPLIAAVEGWALGGGFEIVLACDLVVAGRGARFGLPEV
RRGLAARGGGAFRLPRRLPHMLAMELLLTGAPLEAERAAHFGLVNRLVDDGGALDAALALAGVVAESAPMSVRASKQVAV
DSADWPVSEGFDQQRALFEPVFASSDAAEGVAAFRERRAPVWRDA

Sequences:

>Translated_285_residues
MTACGAPGDANARTMFSIENLSKEPPLPASAPSDDVLVRREDGVLTITINRPDQRNAVNGEVSRGIAAALDLLDSTPELQ
VAVLHGAGPSFCAGMDLKAFAAGEKTSDPVRGFAGLVERPPAKPLIAAVEGWALGGGFEIVLACDLVVAGRGARFGLPEV
RRGLAARGGGAFRLPRRLPHMLAMELLLTGAPLEAERAAHFGLVNRLVDDGGALDAALALAGVVAESAPMSVRASKQVAV
DSADWPVSEGFDQQRALFEPVFASSDAAEGVAAFRERRAPVWRDA
>Mature_284_residues
TACGAPGDANARTMFSIENLSKEPPLPASAPSDDVLVRREDGVLTITINRPDQRNAVNGEVSRGIAAALDLLDSTPELQV
AVLHGAGPSFCAGMDLKAFAAGEKTSDPVRGFAGLVERPPAKPLIAAVEGWALGGGFEIVLACDLVVAGRGARFGLPEVR
RGLAARGGGAFRLPRRLPHMLAMELLLTGAPLEAERAAHFGLVNRLVDDGGALDAALALAGVVAESAPMSVRASKQVAVD
SADWPVSEGFDQQRALFEPVFASSDAAEGVAAFRERRAPVWRDA

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=30.9236947791165, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=31.640625, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=267, Percent_Identity=26.5917602996255, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=29.5454545454545, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI25453481, Length=254, Percent_Identity=23.6220472440945, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51538573, Length=254, Percent_Identity=23.6220472440945, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136756, Length=254, Percent_Identity=24.0157480314961, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI221136753, Length=254, Percent_Identity=24.0157480314961, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI221307494, Length=254, Percent_Identity=24.0157480314961, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757966, Length=247, Percent_Identity=23.8866396761134, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI51538581, Length=247, Percent_Identity=23.8866396761134, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=29.9065420560748, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=29.9065420560748, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=29.9065420560748, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=23.7547892720307, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=23.7547892720307, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=31.25, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=32.2834645669291, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI213417737, Length=253, Percent_Identity=24.901185770751, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=253, Percent_Identity=24.901185770751, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=157, Percent_Identity=31.2101910828025, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116235487, Length=183, Percent_Identity=28.9617486338798, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=38.6100386100386, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=36.1111111111111, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082183, Length=247, Percent_Identity=28.3400809716599, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=33.5907335907336, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=35.4166666666667, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=28.8537549407115, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=32.9545454545455, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=33.1983805668016, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=34.251968503937, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=31.2252964426877, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17534483, Length=229, Percent_Identity=30.1310043668122, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=31.9634703196347, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=287, Percent_Identity=26.1324041811847, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=216, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=205, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=206, Percent_Identity=29.126213592233, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=206, Percent_Identity=29.126213592233, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=24.2718446601942, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357171, Length=281, Percent_Identity=30.9608540925267, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI20129971, Length=236, Percent_Identity=33.4745762711864, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24653477, Length=236, Percent_Identity=33.4745762711864, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24653139, Length=278, Percent_Identity=35.2517985611511, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650670, Length=255, Percent_Identity=29.8039215686275, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=31.7269076305221, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921000, Length=191, Percent_Identity=34.5549738219895, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583077, Length=191, Percent_Identity=34.5549738219895, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583079, Length=191, Percent_Identity=34.5549738219895, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=179, Percent_Identity=27.3743016759777, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 29678; Mature: 29547

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTACGAPGDANARTMFSIENLSKEPPLPASAPSDDVLVRREDGVLTITINRPDQRNAVNG
CCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCC
EVSRGIAAALDLLDSTPELQVAVLHGAGPSFCAGMDLKAFAAGEKTSDPVRGFAGLVERP
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCCCCCCEEECCCCCCCHHHHHHHHHCCC
PAKPLIAAVEGWALGGGFEIVLACDLVVAGRGARFGLPEVRRGLAARGGGAFRLPRRLPH
CCCHHHHHHCCEEECCCEEEEEEEEEHEECCCCCCCCHHHHHHHHHCCCCCEECHHHHHH
MLAMELLLTGAPLEAERAAHFGLVNRLVDDGGALDAALALAGVVAESAPMSVRASKQVAV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCEEEE
DSADWPVSEGFDQQRALFEPVFASSDAAEGVAAFRERRAPVWRDA
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TACGAPGDANARTMFSIENLSKEPPLPASAPSDDVLVRREDGVLTITINRPDQRNAVNG
CCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCC
EVSRGIAAALDLLDSTPELQVAVLHGAGPSFCAGMDLKAFAAGEKTSDPVRGFAGLVERP
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCCCCCCEEECCCCCCCHHHHHHHHHCCC
PAKPLIAAVEGWALGGGFEIVLACDLVVAGRGARFGLPEVRRGLAARGGGAFRLPRRLPH
CCCHHHHHHCCEEECCCEEEEEEEEEHEECCCCCCCCHHHHHHHHHCCCCCEECHHHHHH
MLAMELLLTGAPLEAERAAHFGLVNRLVDDGGALDAALALAGVVAESAPMSVRASKQVAV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCEEEE
DSADWPVSEGFDQQRALFEPVFASSDAAEGVAAFRERRAPVWRDA
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA