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The map label for this gene is fadE25 [H]

Identifier: 158314742

GI number: 158314742

Start: 3440032

End: 3441168

Strand: Direct

Name: fadE25 [H]

Synonym: Franean1_2923

Alternate gene names: 158314742

Gene position: 3440032-3441168 (Clockwise)

Preceding gene: 158314741

Following gene: 158314743

Centisome position: 38.3

GC content: 68.07

Gene sequence:

>1137_bases
ATGCTGTTAGAACTGAGCTCCGAGCAAGGAGTCTTCCGCGCCACAACGGCGAAGTACCTTGCGGAACGAGTGTCGGCCGC
GGCCCTTCGTGCTCTTCGTGACGATCCTGACGGATTTGACCTCGACTACTGGCGCGCCGGCGCGGACCTCGGGTGGACGT
CGTTGCTCATCGGCGAGGACCAGGGCGGGGGGAGCATCAGCGGTGCGCCGCTGGTTGACCTGGGCCTGATCGCCTACGAG
TTCGGCAGGGGCGCGGCACCGGGGCCGCTGGTGCCCACGAACATTGTCGCCGAGAGACTGAGCGTGACCGGCGGCGCGCA
TGCCGCCGTTGTCAAGGAGCTGGCGGCCGGCACCTCGATCGCGTCGTGGTGCCTGGCCGAGCCGGCCTCGAACGGCCATC
CCGGGACCGTGAACACGACGGTACGGACCGACGGACCGGACATCGTGGTAGACGGTGTGAAGCGCCCGGTCGAGGCCGCC
GGGTCGGCCACCCACTTTCTGGTCGTCGGACGAACAGGGACCGGGCTGACACAGGTGCTCGTGCCGGCGGACACGGCGGG
AGTGTCCGTCACGCCGATGCGCGCCGTCGATCTGACCCGTCGCTTCTGCCTTGTCACCTTCGACGGGGTCCGACTGTCGG
CCGACGCGGTGGTCGGTGATCTAGGGGACGCGTCCGATGACGTACGGCGGCAGCTCCAGCTTGCCTTGACGATGACCAGC
GCCGAGTCCGTCGGCGCCATGCAGCGGGCGTTCGACGTCACTCTCACCTGGGCCTTCGATCGCTTCTCGTTCGGACGACC
ACTCGCCACCTACCAGGAGATCAAGCACCGGTTCGCGGACATGGCCACCTGGCTGGAGGCGAGCCACGCCATCACGGATG
CCGCGGTCGCCGCCGTCGAGGCGGGGGCCCCCGAGGCCGACGAACTGGTCAGCGCGGCCAAGTCCTACGTCGGCGAGTAC
GGGTCGGAGTTGCTCCAGGACTGCGTACAGATTCACGGTGGCATCGGTCTCACCTTCGAGCACAACCTCCACCTCTACCT
CCGTCGCCACACGGCGAACCGAACGCTGTTCGGCACCCCCTCGGTCCATCGCCAACGCGTGGCCGACATCGTCGCGCATC
AGGATCGCCCTGCGTAG

Upstream 100 bases:

>100_bases
CGCCCCCGCAGTGCTTTCCTCCGAGGTGCACGTCAGGGGAAAGTCGAGCCACTGTCGCGCAGCAGCCGGCAGAGAGTCCC
TGAGAACGAGATGTGGGTAA

Downstream 100 bases:

>100_bases
CATGCCGGTCCATAGATGTCGGAGGAGATGGAGATCCGGATTTCTCCTCAGAAAAATGAACGGTTCCTCTCTCCCTGCCG
CTGGCCGGCCGGTAAACCTG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLLELSSEQGVFRATTAKYLAERVSAAALRALRDDPDGFDLDYWRAGADLGWTSLLIGEDQGGGSISGAPLVDLGLIAYE
FGRGAAPGPLVPTNIVAERLSVTGGAHAAVVKELAAGTSIASWCLAEPASNGHPGTVNTTVRTDGPDIVVDGVKRPVEAA
GSATHFLVVGRTGTGLTQVLVPADTAGVSVTPMRAVDLTRRFCLVTFDGVRLSADAVVGDLGDASDDVRRQLQLALTMTS
AESVGAMQRAFDVTLTWAFDRFSFGRPLATYQEIKHRFADMATWLEASHAITDAAVAAVEAGAPEADELVSAAKSYVGEY
GSELLQDCVQIHGGIGLTFEHNLHLYLRRHTANRTLFGTPSVHRQRVADIVAHQDRPA

Sequences:

>Translated_378_residues
MLLELSSEQGVFRATTAKYLAERVSAAALRALRDDPDGFDLDYWRAGADLGWTSLLIGEDQGGGSISGAPLVDLGLIAYE
FGRGAAPGPLVPTNIVAERLSVTGGAHAAVVKELAAGTSIASWCLAEPASNGHPGTVNTTVRTDGPDIVVDGVKRPVEAA
GSATHFLVVGRTGTGLTQVLVPADTAGVSVTPMRAVDLTRRFCLVTFDGVRLSADAVVGDLGDASDDVRRQLQLALTMTS
AESVGAMQRAFDVTLTWAFDRFSFGRPLATYQEIKHRFADMATWLEASHAITDAAVAAVEAGAPEADELVSAAKSYVGEY
GSELLQDCVQIHGGIGLTFEHNLHLYLRRHTANRTLFGTPSVHRQRVADIVAHQDRPA
>Mature_378_residues
MLLELSSEQGVFRATTAKYLAERVSAAALRALRDDPDGFDLDYWRAGADLGWTSLLIGEDQGGGSISGAPLVDLGLIAYE
FGRGAAPGPLVPTNIVAERLSVTGGAHAAVVKELAAGTSIASWCLAEPASNGHPGTVNTTVRTDGPDIVVDGVKRPVEAA
GSATHFLVVGRTGTGLTQVLVPADTAGVSVTPMRAVDLTRRFCLVTFDGVRLSADAVVGDLGDASDDVRRQLQLALTMTS
AESVGAMQRAFDVTLTWAFDRFSFGRPLATYQEIKHRFADMATWLEASHAITDAAVAAVEAGAPEADELVSAAKSYVGEY
GSELLQDCVQIHGGIGLTFEHNLHLYLRRHTANRTLFGTPSVHRQRVADIVAHQDRPA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=237, Percent_Identity=28.2700421940928, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4557231, Length=382, Percent_Identity=23.5602094240838, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI187960098, Length=382, Percent_Identity=23.5602094240838, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI7656849, Length=379, Percent_Identity=25.065963060686, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI4557233, Length=267, Percent_Identity=25.4681647940075, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4501857, Length=354, Percent_Identity=21.4689265536723, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI21361497, Length=380, Percent_Identity=22.1052631578947, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI76496475, Length=345, Percent_Identity=22.6086956521739, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557235, Length=345, Percent_Identity=22.6086956521739, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786223, Length=389, Percent_Identity=24.4215938303342, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081958, Length=375, Percent_Identity=22.6666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534899, Length=382, Percent_Identity=25.6544502617801, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17570075, Length=387, Percent_Identity=25.0645994832041, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17569725, Length=262, Percent_Identity=25.5725190839695, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17508101, Length=264, Percent_Identity=26.5151515151515, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17506239, Length=269, Percent_Identity=24.1635687732342, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533517, Length=339, Percent_Identity=22.7138643067847, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990804, Length=265, Percent_Identity=24.9056603773585, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71985184, Length=340, Percent_Identity=22.6470588235294, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17505929, Length=244, Percent_Identity=26.6393442622951, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538396, Length=263, Percent_Identity=23.1939163498099, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646207, Length=267, Percent_Identity=28.0898876404494, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666513, Length=378, Percent_Identity=24.3386243386243, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24660351, Length=257, Percent_Identity=24.9027237354086, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356377, Length=336, Percent_Identity=22.9166666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355753, Length=337, Percent_Identity=22.2551928783383, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 39959; Mature: 39959

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLELSSEQGVFRATTAKYLAERVSAAALRALRDDPDGFDLDYWRAGADLGWTSLLIGED
CEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECC
QGGGSISGAPLVDLGLIAYEFGRGAAPGPLVPTNIVAERLSVTGGAHAAVVKELAAGTSI
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHH
ASWCLAEPASNGHPGTVNTTVRTDGPDIVVDGVKRPVEAAGSATHFLVVGRTGTGLTQVL
HHHHHCCCCCCCCCCEEEEEEECCCCCEEECCCCCCHHHCCCCEEEEEEECCCCCCEEEE
VPADTAGVSVTPMRAVDLTRRFCLVTFDGVRLSADAVVGDLGDASDDVRRQLQLALTMTS
EECCCCCCEECCHHHHHHHHEEEEEEECCEEEEHHHHCCCCCCCCHHHHHHHHHHHEECC
AESVGAMQRAFDVTLTWAFDRFSFGRPLATYQEIKHRFADMATWLEASHAITDAAVAAVE
HHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAPEADELVSAAKSYVGEYGSELLQDCVQIHGGIGLTFEHNLHLYLRRHTANRTLFGTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEECCCCEEECCC
SVHRQRVADIVAHQDRPA
CHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLELSSEQGVFRATTAKYLAERVSAAALRALRDDPDGFDLDYWRAGADLGWTSLLIGED
CEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECC
QGGGSISGAPLVDLGLIAYEFGRGAAPGPLVPTNIVAERLSVTGGAHAAVVKELAAGTSI
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHH
ASWCLAEPASNGHPGTVNTTVRTDGPDIVVDGVKRPVEAAGSATHFLVVGRTGTGLTQVL
HHHHHCCCCCCCCCCEEEEEEECCCCCEEECCCCCCHHHCCCCEEEEEEECCCCCCEEEE
VPADTAGVSVTPMRAVDLTRRFCLVTFDGVRLSADAVVGDLGDASDDVRRQLQLALTMTS
EECCCCCCEECCHHHHHHHHEEEEEEECCEEEEHHHHCCCCCCCCHHHHHHHHHHHEECC
AESVGAMQRAFDVTLTWAFDRFSFGRPLATYQEIKHRFADMATWLEASHAITDAAVAAVE
HHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAPEADELVSAAKSYVGEYGSELLQDCVQIHGGIGLTFEHNLHLYLRRHTANRTLFGTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEECCCCEEECCC
SVHRQRVADIVAHQDRPA
CHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]