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The map label for this gene is hrdB [H]

Identifier: 158313963

GI number: 158313963

Start: 2557715

End: 2558944

Strand: Direct

Name: hrdB [H]

Synonym: Franean1_2129

Alternate gene names: 158313963

Gene position: 2557715-2558944 (Clockwise)

Preceding gene: 158313962

Following gene: 158313964

Centisome position: 28.48

GC content: 66.91

Gene sequence:

>1230_bases
GTGCCGCGTAACCTGGGTCGATCCCGAGAAGGAAGTGACGAGATTCCGCCCATGAGTCCTACCGTCCTACCCCGCGAGGC
CCAGGTGGACGAGGTCAAGGACCTCATCACCCGTGGCAAGGAGATCGGTTTCCTCACCACCGAGGACGTCACGGTCGCGA
TTCAGGCGGCCGAGCTGCCCCCCGAGCAGGCCGAGACCGTCCTGCAGGTGCTCAACGACGAGGGCATCGAGGTTCTCGAG
GCCGGGGGGGAGAACGCCGACGAGGCGGATCTGCTGGCCCGTCGCCGCCGCGAGGAGGAGGAGCTCGCGCTCAAGGCGCC
GACCTCCGACCCGGTGCGGATGTACCTCAAGGAGATCGGCAAGGTACCGCTGCTCACCGCGGAGGAGGAGGTCGACCTCG
CCAAGCGGATCGAGGCGGGCCTGTTCGCCTCCGAGAAGCTCGCGGTGGCGACGAAGAAGACCTCCCCGCAGATGCGGCGG
GATCTCGAGGCCATCGAGCGGGACGGTCAGATCGCCAAGCGGAAGCTCGTCGAGGCGAACCTGCGGCTCGTCGTCTCGAT
CGCCAAGCGCTACGTGGGGCGCGGGATGCTCTTCCTGGACCTCATTCAGGAGGGCAACCTCGGCCTCATCCGCGCGGTCG
AGAAGTTCGACTACACCAAGGGCTACAAGTTCTCCACCTACGCCACCTGGTGGATCCGGCAGGCCATCACCCGCGCCATC
GCGGACCAGGCCCGGACGATCCGCATCCCGGTGCACATGGTCGAGACGATCAACAAGCTGATCCGCATCCAGCGCCAGCT
CCTGCAGGACCTCGGCCGGGAGCCGAGCCCGGAGGAGATCGCCAAGGAGATGGACCTCACGCCCGACAAGGTGCGGGAGA
TCCTCAAAGTGTCGCAGGAGCCGGTCTCCCTGGAGACGCCGATCGGTGAGGAGGAGGACTCCCACCTCGGCGACTTCATC
GAGGACTGCGACGCGGTCGTCCCGGTCGACGCCGCCAGCTTCATCCTCCTGCAGGAGCAGCTCGACTCCGTGCTGCACAC
GCTGTCCGACCGTGAGAAGAAGGTGATCCAGCTACGCTTCGGCCTCACCGACGGCCATCCGCGCACGCTGGAGGAGGTCG
GCCGCGAGTTCGGGGTCACCCGGGAGCGCATCCGGCAGATCGAGTCGAAGACGCTGTCGAAGCTGCGCCACCCGTCCCGA
TCCCAGAAGCTGCGCGACTACCTGGAGTAG

Upstream 100 bases:

>100_bases
CGACTGAACCCGGCATCCGACACCGATGCCTTCAACCGTGCGTTCGGTGAGCTGATTGCTTTGGAACAGCACAAGCTCCA
ACTCCGGGAGCGGGGCGTAG

Downstream 100 bases:

>100_bases
TCGAACTCGTAGTAGTCATTCGTTGACTTAGCGTGTTCGGCCTGTCAGGGTCGGACCTTGTGACGCAGCGGTCAGGCAGT
CCGGAAGGATCTGCGGCTCC

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MPRNLGRSREGSDEIPPMSPTVLPREAQVDEVKDLITRGKEIGFLTTEDVTVAIQAAELPPEQAETVLQVLNDEGIEVLE
AGGENADEADLLARRRREEEELALKAPTSDPVRMYLKEIGKVPLLTAEEEVDLAKRIEAGLFASEKLAVATKKTSPQMRR
DLEAIERDGQIAKRKLVEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAI
ADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIAKEMDLTPDKVREILKVSQEPVSLETPIGEEEDSHLGDFI
EDCDAVVPVDAASFILLQEQLDSVLHTLSDREKKVIQLRFGLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSR
SQKLRDYLE

Sequences:

>Translated_409_residues
MPRNLGRSREGSDEIPPMSPTVLPREAQVDEVKDLITRGKEIGFLTTEDVTVAIQAAELPPEQAETVLQVLNDEGIEVLE
AGGENADEADLLARRRREEEELALKAPTSDPVRMYLKEIGKVPLLTAEEEVDLAKRIEAGLFASEKLAVATKKTSPQMRR
DLEAIERDGQIAKRKLVEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAI
ADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIAKEMDLTPDKVREILKVSQEPVSLETPIGEEEDSHLGDFI
EDCDAVVPVDAASFILLQEQLDSVLHTLSDREKKVIQLRFGLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSR
SQKLRDYLE
>Mature_408_residues
PRNLGRSREGSDEIPPMSPTVLPREAQVDEVKDLITRGKEIGFLTTEDVTVAIQAAELPPEQAETVLQVLNDEGIEVLEA
GGENADEADLLARRRREEEELALKAPTSDPVRMYLKEIGKVPLLTAEEEVDLAKRIEAGLFASEKLAVATKKTSPQMRRD
LEAIERDGQIAKRKLVEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAIA
DQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIAKEMDLTPDKVREILKVSQEPVSLETPIGEEEDSHLGDFIE
DCDAVVPVDAASFILLQEQLDSVLHTLSDREKKVIQLRFGLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRS
QKLRDYLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=64.7058823529412, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1789098, Length=309, Percent_Identity=43.042071197411, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789871, Length=314, Percent_Identity=27.0700636942675, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 46310; Mature: 46178

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNLGRSREGSDEIPPMSPTVLPREAQVDEVKDLITRGKEIGFLTTEDVTVAIQAAELP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCEEEEEEECCCC
PEQAETVLQVLNDEGIEVLEAGGENADEADLLARRRREEEELALKAPTSDPVRMYLKEIG
HHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHC
KVPLLTAEEEVDLAKRIEAGLFASEKLAVATKKTSPQMRRDLEAIERDGQIAKRKLVEAN
CCCEEECHHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAI
HHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIAKEMDLTPDKVREILKVSQE
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCC
PVSLETPIGEEEDSHLGDFIEDCDAVVPVDAASFILLQEQLDSVLHTLSDREKKVIQLRF
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
GLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQKLRDYLE
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
PRNLGRSREGSDEIPPMSPTVLPREAQVDEVKDLITRGKEIGFLTTEDVTVAIQAAELP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCEEEEEEECCCC
PEQAETVLQVLNDEGIEVLEAGGENADEADLLARRRREEEELALKAPTSDPVRMYLKEIG
HHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHC
KVPLLTAEEEVDLAKRIEAGLFASEKLAVATKKTSPQMRRDLEAIERDGQIAKRKLVEAN
CCCEEECHHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAI
HHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIAKEMDLTPDKVREILKVSQE
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCC
PVSLETPIGEEEDSHLGDFIEDCDAVVPVDAASFILLQEQLDSVLHTLSDREKKVIQLRF
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
GLTDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQKLRDYLE
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]