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The map label for this gene is dnaG [H]

Identifier: 158313962

GI number: 158313962

Start: 2555851

End: 2557725

Strand: Direct

Name: dnaG [H]

Synonym: Franean1_2128

Alternate gene names: 158313962

Gene position: 2555851-2557725 (Clockwise)

Preceding gene: 158313961

Following gene: 158313963

Centisome position: 28.46

GC content: 71.31

Gene sequence:

>1875_bases
GTGGCCGGACGCATCCGCGACGCTGACATCGCTCTCGTACGGGAGCGCTCACTCATCGCGGACGTCATCGGTGACCACGT
GCAGCTCCGTCCGGTCGGCGGTGGCAGCCTGGTCGGCCTGTGCCCGTTCCACGACGAGAAGTCACCGTCGTTCAACGTGC
GGCCCTCGGTCGGGTTCTTCCACTGCTTTGGCTGCAACGAGGGCGGGGACGTCTACGCCTTCATCCGCAAGATCGACGGG
TTGACCTTCCCGGAGGCCGTCGAGCGGCTCGCCCGGCGGGCCGGCATCACGCTGACCTACGAGGGCGGGGGCACCACCAG
CCGCGCGGTGACGAGCCAGCGCCAGCGGCTGCTGGACGCCCACCGGCTGGCCGCCGAGTTCTACGTCGAACAGCTCCACT
CCTCCCCGGACGCCCGGGCCGGCTGGGAGTTCCTGGCCTCGCGTGGCTTCGACCGGGCGGCGGCGGAACAGTTCGGTCTC
GGCTACGCGCCGTCGTCCTGGGACGCGCTGACCAGGCACCTGCTCGCCCGCCGGTTCACCGCCGAGGAGCTGGAGCTGGG
CGGGCTGGCGAAGCGGGGCGGCCGCGGCGGCCTCATCGACCGGTTCCACCACCGCCTGCTGTGGCCGATCCGTGACCTCG
GCGGCGAGGTCGTCGGTTTCGGTGGCCGGCGCCTCGCCGAGGGCCCCGATGCCGGTCCCAAGTACCTCAACACCGCCGAG
ACGCCGCTGTTCAAGAAGGCGAAGCTCCTCTACGGCGCCGACCTGGCCCGCCGCGAGATCGCGCGCCGCTACCAGGTTGT
CGTCGTCGAGGGCTACACCGACGTCATGGCGTGCCACCTGGCCGGGGTGCCGACGGCGGTCGCGAGCTGCGGAACCTCCT
TCGGTGCCGACCACGTCGCGATCGTCCGCCGGCTGCTGATGGACTCCGACGCCCGCACCGGCGAGGTCATCTTCACCTTC
GACGGGGACAAGGCGGGCCAGGCCGCCGCCCTGCGGGCCTTCGAGTACGAGGACCGCTTCGTCACCCAGACCTTCGTCGC
GGTGCAGCCGGACGGCCTCGACCCCTGCGACCTGTGGACGCAGCACGGTGACGCGGCCGTGCGAGACCTGGTGGCCAGCC
GCCAGCCGCTGTTCGAGTTCGCCATCCGCGGCGTGATCGACCGCTACGACCTCAACACCACCGAGGGCCGCATCGCCGCG
CTGGACGCGGCAGCCCCGCTGGTGAACCGGCTCAAGGACGCAGGCATGCGGCACCGGTACGCGGTGAACCTCGACCGCTG
GCTCGGCTTCCTCGACGAGCGGTTCGTGGTCGAGCGGGTGGGGCAGCACCGGTCGCAGGCCGGGGCGGCCGAGGGCGTCG
GCGCGCGCCGGCGGCTGGACCCGGCGCCGGACGATGCGACCGCCATCGTCGAACGGGAGACCCTCAAACTCGCCCTGCAA
CATCCGGGGCTGGCCGGACCGATGTTCGACACGCTGTCCACGGAGCTGTTCACTGTTCCCGCGCACCGGGCCGTCCGGGA
GGCGATCGCCGTGGCGGGCGGGGTGTGCGCGGGTCTGTACGCGGCCGGTGACCCGTCCGGCTGGGTTGCGGCGGTGGTGG
ACGCGACGCCGGACGAGGAGCTCCGGCGGTTCGTCCTCGCGCTCGCTGTCGAGGGGGTGCTGTGCGACCACGATGTGGAC
GACCGCTACGTCGACGATCAGATGTCGCGTGTGCAGGAGCTGCATGTGACCCGTCGGATCCGGGAGCTCAAGTCGCGGGT
GCAGCGACTGAACCCGGCATCCGACACCGATGCCTTCAACCGTGCGTTCGGTGAGCTGATTGCTTTGGAACAGCACAAGC
TCCAACTCCGGGAGCGGGGCGTAGGTGCCGCGTAA

Upstream 100 bases:

>100_bases
GGGCGCGCCGTCTATCCAGTGACTCGGATCAGTGACTCGGACCAGTGACCTCGGAGCCCGCCGCTGGTGGGTCCGCCGGA
CGTGAGCAGGGAGGGTATCG

Downstream 100 bases:

>100_bases
CCTGGGTCGATCCCGAGAAGGAAGTGACGAGATTCCGCCCATGAGTCCTACCGTCCTACCCCGCGAGGCCCAGGTGGACG
AGGTCAAGGACCTCATCACC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MAGRIRDADIALVRERSLIADVIGDHVQLRPVGGGSLVGLCPFHDEKSPSFNVRPSVGFFHCFGCNEGGDVYAFIRKIDG
LTFPEAVERLARRAGITLTYEGGGTTSRAVTSQRQRLLDAHRLAAEFYVEQLHSSPDARAGWEFLASRGFDRAAAEQFGL
GYAPSSWDALTRHLLARRFTAEELELGGLAKRGGRGGLIDRFHHRLLWPIRDLGGEVVGFGGRRLAEGPDAGPKYLNTAE
TPLFKKAKLLYGADLARREIARRYQVVVVEGYTDVMACHLAGVPTAVASCGTSFGADHVAIVRRLLMDSDARTGEVIFTF
DGDKAGQAAALRAFEYEDRFVTQTFVAVQPDGLDPCDLWTQHGDAAVRDLVASRQPLFEFAIRGVIDRYDLNTTEGRIAA
LDAAAPLVNRLKDAGMRHRYAVNLDRWLGFLDERFVVERVGQHRSQAGAAEGVGARRRLDPAPDDATAIVERETLKLALQ
HPGLAGPMFDTLSTELFTVPAHRAVREAIAVAGGVCAGLYAAGDPSGWVAAVVDATPDEELRRFVLALAVEGVLCDHDVD
DRYVDDQMSRVQELHVTRRIRELKSRVQRLNPASDTDAFNRAFGELIALEQHKLQLRERGVGAA

Sequences:

>Translated_624_residues
MAGRIRDADIALVRERSLIADVIGDHVQLRPVGGGSLVGLCPFHDEKSPSFNVRPSVGFFHCFGCNEGGDVYAFIRKIDG
LTFPEAVERLARRAGITLTYEGGGTTSRAVTSQRQRLLDAHRLAAEFYVEQLHSSPDARAGWEFLASRGFDRAAAEQFGL
GYAPSSWDALTRHLLARRFTAEELELGGLAKRGGRGGLIDRFHHRLLWPIRDLGGEVVGFGGRRLAEGPDAGPKYLNTAE
TPLFKKAKLLYGADLARREIARRYQVVVVEGYTDVMACHLAGVPTAVASCGTSFGADHVAIVRRLLMDSDARTGEVIFTF
DGDKAGQAAALRAFEYEDRFVTQTFVAVQPDGLDPCDLWTQHGDAAVRDLVASRQPLFEFAIRGVIDRYDLNTTEGRIAA
LDAAAPLVNRLKDAGMRHRYAVNLDRWLGFLDERFVVERVGQHRSQAGAAEGVGARRRLDPAPDDATAIVERETLKLALQ
HPGLAGPMFDTLSTELFTVPAHRAVREAIAVAGGVCAGLYAAGDPSGWVAAVVDATPDEELRRFVLALAVEGVLCDHDVD
DRYVDDQMSRVQELHVTRRIRELKSRVQRLNPASDTDAFNRAFGELIALEQHKLQLRERGVGAA
>Mature_623_residues
AGRIRDADIALVRERSLIADVIGDHVQLRPVGGGSLVGLCPFHDEKSPSFNVRPSVGFFHCFGCNEGGDVYAFIRKIDGL
TFPEAVERLARRAGITLTYEGGGTTSRAVTSQRQRLLDAHRLAAEFYVEQLHSSPDARAGWEFLASRGFDRAAAEQFGLG
YAPSSWDALTRHLLARRFTAEELELGGLAKRGGRGGLIDRFHHRLLWPIRDLGGEVVGFGGRRLAEGPDAGPKYLNTAET
PLFKKAKLLYGADLARREIARRYQVVVVEGYTDVMACHLAGVPTAVASCGTSFGADHVAIVRRLLMDSDARTGEVIFTFD
GDKAGQAAALRAFEYEDRFVTQTFVAVQPDGLDPCDLWTQHGDAAVRDLVASRQPLFEFAIRGVIDRYDLNTTEGRIAAL
DAAAPLVNRLKDAGMRHRYAVNLDRWLGFLDERFVVERVGQHRSQAGAAEGVGARRRLDPAPDDATAIVERETLKLALQH
PGLAGPMFDTLSTELFTVPAHRAVREAIAVAGGVCAGLYAAGDPSGWVAAVVDATPDEELRRFVLALAVEGVLCDHDVDD
RYVDDQMSRVQELHVTRRIRELKSRVQRLNPASDTDAFNRAFGELIALEQHKLQLRERGVGAA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=629, Percent_Identity=30.8426073131955, Blast_Score=242, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68311; Mature: 68180

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIRDADIALVRERSLIADVIGDHVQLRPVGGGSLVGLCPFHDEKSPSFNVRPSVGFF
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEE
HCFGCNEGGDVYAFIRKIDGLTFPEAVERLARRAGITLTYEGGGTTSRAVTSQRQRLLDA
EEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
HRLAAEFYVEQLHSSPDARAGWEFLASRGFDRAAAEQFGLGYAPSSWDALTRHLLARRFT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AEELELGGLAKRGGRGGLIDRFHHRLLWPIRDLGGEVVGFGGRRLAEGPDAGPKYLNTAE
HHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCC
TPLFKKAKLLYGADLARREIARRYQVVVVEGYTDVMACHLAGVPTAVASCGTSFGADHVA
CCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCHHHH
IVRRLLMDSDARTGEVIFTFDGDKAGQAAALRAFEYEDRFVTQTFVAVQPDGLDPCDLWT
HHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCHHEEEEEEEECCCCCCHHHHHH
QHGDAAVRDLVASRQPLFEFAIRGVIDRYDLNTTEGRIAALDAAAPLVNRLKDAGMRHRY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEE
AVNLDRWLGFLDERFVVERVGQHRSQAGAAEGVGARRRLDPAPDDATAIVERETLKLALQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
HPGLAGPMFDTLSTELFTVPAHRAVREAIAVAGGVCAGLYAAGDPSGWVAAVVDATPDEE
CCCCCCCHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
LRRFVLALAVEGVLCDHDVDDRYVDDQMSRVQELHVTRRIRELKSRVQRLNPASDTDAFN
HHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAFGELIALEQHKLQLRERGVGAA
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AGRIRDADIALVRERSLIADVIGDHVQLRPVGGGSLVGLCPFHDEKSPSFNVRPSVGFF
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEE
HCFGCNEGGDVYAFIRKIDGLTFPEAVERLARRAGITLTYEGGGTTSRAVTSQRQRLLDA
EEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
HRLAAEFYVEQLHSSPDARAGWEFLASRGFDRAAAEQFGLGYAPSSWDALTRHLLARRFT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AEELELGGLAKRGGRGGLIDRFHHRLLWPIRDLGGEVVGFGGRRLAEGPDAGPKYLNTAE
HHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCC
TPLFKKAKLLYGADLARREIARRYQVVVVEGYTDVMACHLAGVPTAVASCGTSFGADHVA
CCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCHHHH
IVRRLLMDSDARTGEVIFTFDGDKAGQAAALRAFEYEDRFVTQTFVAVQPDGLDPCDLWT
HHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCHHEEEEEEEECCCCCCHHHHHH
QHGDAAVRDLVASRQPLFEFAIRGVIDRYDLNTTEGRIAALDAAAPLVNRLKDAGMRHRY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEE
AVNLDRWLGFLDERFVVERVGQHRSQAGAAEGVGARRRLDPAPDDATAIVERETLKLALQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
HPGLAGPMFDTLSTELFTVPAHRAVREAIAVAGGVCAGLYAAGDPSGWVAAVVDATPDEE
CCCCCCCHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
LRRFVLALAVEGVLCDHDVDDRYVDDQMSRVQELHVTRRIRELKSRVQRLNPASDTDAFN
HHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAFGELIALEQHKLQLRERGVGAA
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]