Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 158313874

GI number: 158313874

Start: 2451468

End: 2452904

Strand: Direct

Name: braE [H]

Synonym: Franean1_2039

Alternate gene names: 158313874

Gene position: 2451468-2452904 (Clockwise)

Preceding gene: 158313873

Following gene: 158313875

Centisome position: 27.29

GC content: 74.81

Gene sequence:

>1437_bases
ATGGCCCGGCCGTGGGCGGCCCGGTCGGGGGGTGTGCCCTGGCTGGTTGTGGCCAGGTCGCCGGCGGCCCGGCGGCTGGG
TGCCTGCCTGTTCCTGGCCGTGATCGCCGCGGTGGTGACGGGCCCGTCCGGGGACGCCGACCGCCCACTGGCGGGGATCG
AAGGCTCGCTCACGCCCGCACGGGCGGCCGGGTTCGCTGCCGGGGGACTCGCGCTGTGGGCGCTGTCGATCGGTGCCGAC
CGGCTGACGACCCGGCTCCGGTCCGCCGGCCACGGCGCCGCGCTGGGCGGCGGTCGCCTGGCCGCGCTGGGCGGCGGGCT
GTGGGTGGCGCCGGCCGGTGCCGCGTGGGCGTCGTTCCGCCGGGGCCGGTGGGCGGCGCCGGCGGGCAACCTGGCGCTGC
TCGCCGTCGCCGTGGCGGTCCCGCCGCTGCTGTCCACGGCGGCCCAGCAGGCGCTGGTGAGCGAGATCGGGATCTACGCC
CTGCTCGCCCTCGGCCTGAACGTCGTCGTCGGCTACGCCGGACTGCTCGATCTCGGCTACATCGCCTTCTTCGCGATCGG
CGCCTACACCACGGCCTACGTGACGTCCGCGGACGCGATGCCCTGGCACGCGCCCTGGCAGGTCAACCCGTTCTTCGCGC
TACCCGTCGCACTGGTCGTGGCCGCCGTCGCGGGTGTCGTCCTGGGCGGCCCCACGCTGCGCCTGCGCGGGGACTACCTG
GCCATCGTCACCCTCGGGTTCGGCGAGATCGTCCAGTTGCTCGCGAACAACGCCGACGGCGTCACCGGCGGCCCACGCGG
CGTGTTCGGCCTGCCGCCGCTGTCGGTCGACCTGCCGGGTGTGCACTACTCCTGGGGCCTGAGGCCGTTGCCCTACTACT
ACCTACTCGTCGTCCTGATCGTGCTCGTCATGGTGGTCTTCGGCCGGTGGGAGCGCTCCCGGACCGGCCGGGCCTGGACG
GCGATCCGCCAGGACGAGGTGGCCGCCGAGGCCACGGGCGTGGCGACGATGCGGATGAAGCTGCTGGCCTTCGCCGTCGG
CGCGTCCGTGTCGGGCTTCGCCGGCGTGGTCCTGGCGACCAAGCAGTTCTTCAACCCGCAGACGTTCGGCCTGCAGTCCT
CCCTCCTCGTGCTGACCATCGTGATCTTCGGTGGTATGGGCTCGCGGCTCGGCGTGGTGCTCGGGGCGCTGGTCCTGCAG
GGGACGGCCTTCCTCCTGCGCGACACGGTCCCGCCCGCGGACCGCTACATCTACTTCGGCGCAGTGGTGATCCTGATGAT
GATCTTCCGTCCGCAGGGCCTGCTGCCGCCGCGGGGCCCGCGGGCACCCGTCAGCCCCGGCCCGGCCGCCGCCCCGGAGC
ACCCGGCGGTTCTCACCGAGCGCGCCGGAGCCTCGGGACCAGCCGGGCCCGCAGCGTCGGCCGAGGCGGCGCGGTGA

Upstream 100 bases:

>100_bases
CCGGCCCGCGCCGCGGCGGTCGCCCGCGGTCCGGTCGCATTCCGTCCGGGCACGGAAGGCCCAGTCGCGGCGGGCCCAGC
TGCGAGAACTGCGGTCGCGG

Downstream 100 bases:

>100_bases
CCGGCCGGCCCATCCCCGGCCCGCGGCCGTCGACGACCCTCGGGCCGGTCCTGGACGTCCGCGACGTCACCCTGCGCTTC
GGCGGGCTGACCAGCCTGGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MARPWAARSGGVPWLVVARSPAARRLGACLFLAVIAAVVTGPSGDADRPLAGIEGSLTPARAAGFAAGGLALWALSIGAD
RLTTRLRSAGHGAALGGGRLAALGGGLWVAPAGAAWASFRRGRWAAPAGNLALLAVAVAVPPLLSTAAQQALVSEIGIYA
LLALGLNVVVGYAGLLDLGYIAFFAIGAYTTAYVTSADAMPWHAPWQVNPFFALPVALVVAAVAGVVLGGPTLRLRGDYL
AIVTLGFGEIVQLLANNADGVTGGPRGVFGLPPLSVDLPGVHYSWGLRPLPYYYLLVVLIVLVMVVFGRWERSRTGRAWT
AIRQDEVAAEATGVATMRMKLLAFAVGASVSGFAGVVLATKQFFNPQTFGLQSSLLVLTIVIFGGMGSRLGVVLGALVLQ
GTAFLLRDTVPPADRYIYFGAVVILMMIFRPQGLLPPRGPRAPVSPGPAAAPEHPAVLTERAGASGPAGPAASAEAAR

Sequences:

>Translated_478_residues
MARPWAARSGGVPWLVVARSPAARRLGACLFLAVIAAVVTGPSGDADRPLAGIEGSLTPARAAGFAAGGLALWALSIGAD
RLTTRLRSAGHGAALGGGRLAALGGGLWVAPAGAAWASFRRGRWAAPAGNLALLAVAVAVPPLLSTAAQQALVSEIGIYA
LLALGLNVVVGYAGLLDLGYIAFFAIGAYTTAYVTSADAMPWHAPWQVNPFFALPVALVVAAVAGVVLGGPTLRLRGDYL
AIVTLGFGEIVQLLANNADGVTGGPRGVFGLPPLSVDLPGVHYSWGLRPLPYYYLLVVLIVLVMVVFGRWERSRTGRAWT
AIRQDEVAAEATGVATMRMKLLAFAVGASVSGFAGVVLATKQFFNPQTFGLQSSLLVLTIVIFGGMGSRLGVVLGALVLQ
GTAFLLRDTVPPADRYIYFGAVVILMMIFRPQGLLPPRGPRAPVSPGPAAAPEHPAVLTERAGASGPAGPAASAEAAR
>Mature_477_residues
ARPWAARSGGVPWLVVARSPAARRLGACLFLAVIAAVVTGPSGDADRPLAGIEGSLTPARAAGFAAGGLALWALSIGADR
LTTRLRSAGHGAALGGGRLAALGGGLWVAPAGAAWASFRRGRWAAPAGNLALLAVAVAVPPLLSTAAQQALVSEIGIYAL
LALGLNVVVGYAGLLDLGYIAFFAIGAYTTAYVTSADAMPWHAPWQVNPFFALPVALVVAAVAGVVLGGPTLRLRGDYLA
IVTLGFGEIVQLLANNADGVTGGPRGVFGLPPLSVDLPGVHYSWGLRPLPYYYLLVVLIVLVMVVFGRWERSRTGRAWTA
IRQDEVAAEATGVATMRMKLLAFAVGASVSGFAGVVLATKQFFNPQTFGLQSSLLVLTIVIFGGMGSRLGVVLGALVLQG
TAFLLRDTVPPADRYIYFGAVVILMMIFRPQGLLPPRGPRAPVSPGPAAAPEHPAVLTERAGASGPAGPAASAEAAR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=317, Percent_Identity=41.3249211356467, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 49011; Mature: 48880

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPWAARSGGVPWLVVARSPAARRLGACLFLAVIAAVVTGPSGDADRPLAGIEGSLTPA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RAAGFAAGGLALWALSIGADRLTTRLRSAGHGAALGGGRLAALGGGLWVAPAGAAWASFR
HHHCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCHHHHHHH
RGRWAAPAGNLALLAVAVAVPPLLSTAAQQALVSEIGIYALLALGLNVVVGYAGLLDLGY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFFAIGAYTTAYVTSADAMPWHAPWQVNPFFALPVALVVAAVAGVVLGGPTLRLRGDYL
HHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
AIVTLGFGEIVQLLANNADGVTGGPRGVFGLPPLSVDLPGVHYSWGLRPLPYYYLLVVLI
EEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHH
VLVMVVFGRWERSRTGRAWTAIRQDEVAAEATGVATMRMKLLAFAVGASVSGFAGVVLAT
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KQFFNPQTFGLQSSLLVLTIVIFGGMGSRLGVVLGALVLQGTAFLLRDTVPPADRYIYFG
HHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHH
AVVILMMIFRPQGLLPPRGPRAPVSPGPAAAPEHPAVLTERAGASGPAGPAASAEAAR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARPWAARSGGVPWLVVARSPAARRLGACLFLAVIAAVVTGPSGDADRPLAGIEGSLTPA
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RAAGFAAGGLALWALSIGADRLTTRLRSAGHGAALGGGRLAALGGGLWVAPAGAAWASFR
HHHCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCHHHHHHH
RGRWAAPAGNLALLAVAVAVPPLLSTAAQQALVSEIGIYALLALGLNVVVGYAGLLDLGY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFFAIGAYTTAYVTSADAMPWHAPWQVNPFFALPVALVVAAVAGVVLGGPTLRLRGDYL
HHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
AIVTLGFGEIVQLLANNADGVTGGPRGVFGLPPLSVDLPGVHYSWGLRPLPYYYLLVVLI
EEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHH
VLVMVVFGRWERSRTGRAWTAIRQDEVAAEATGVATMRMKLLAFAVGASVSGFAGVVLAT
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KQFFNPQTFGLQSSLLVLTIVIFGGMGSRLGVVLGALVLQGTAFLLRDTVPPADRYIYFG
HHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHH
AVVILMMIFRPQGLLPPRGPRAPVSPGPAAAPEHPAVLTERAGASGPAGPAASAEAAR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]